Restriction map for
pF1KE0740
(Circular) (Six-base) MAPSORT of: pF1KE0740.seq Check: 7985 from: 1 to: 4385
pF1KE0740 4385 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 4225 4225
Size: 4385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 585 1005 2210 2642 3389 3657 431
Size: 154 420 1205 432 747 268 1159
Fragments arranged by size:
1205 1159 747 432 420 268 154
AflIII A'CryG_T
Cuts at: 829 1737 2841 3818 829
Size: 908 1104 977 1396
Fragments arranged by size:
1396 1104 977 908
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AlwNI CAG_nnn'CTG
Cuts at: 1690 3257 1690
Size: 1567 2818
ApaLI G'TGCA_C
Cuts at: 3155 3155
Size: 4385
ApoI r'AATT_y
Cuts at: 536 635 1117 1332 1389 3738 3994 536
Size: 99 482 215 57 2349 256 927
Fragments arranged by size:
2349 927 482 256 215 99 57
AseI AT'TA_AT
Cuts at: 20 290 20
Size: 270 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 130 1405 130
Size: 1275 3110
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 962 995 962
Size: 33 4352
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 1401 1815 2063 2098 1401
Size: 414 248 35 3688
Fragments arranged by size:
3688 414 248 35
BanII G_rGCy'C
Cuts at: 1399 2429 1399
Size: 1030 3355
BbeI G_GCGC'C
Cuts at: 1819 2067 1819
Size: 248 4137
BbvCI CC'TCA_GC
Cuts at: 220 220
Size: 4385
BciVI GTATCCnnnnn_n'
Cuts at: 2283 3044 4385 2283
Size: 761 1341 2283
Fragments arranged by size:
2283 1341 761
BclI T'GATC_A
Cuts at: 1905 1905
Size: 4385
BglII A'GATC_T
Cuts at: 680 1900 3937 680
Size: 1220 2037 1128
Fragments arranged by size:
2037 1220 1128
BlpI GC'TnA_GC
Cuts at: 1497 1497
Size: 4385
Bme1580I G_kGCm'C
Cuts at: 2010 2103 3159 2010
Size: 93 1056 3236
Fragments arranged by size:
3236 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 984 1597 1678 2013 4157 984
Size: 613 81 335 2144 1212
Fragments arranged by size:
2144 1212 613 335 81
BmtI G_CTAG'C
Cuts at: 1288 1288
Size: 4385
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4385
Bpu10I CC'TnA_GC
Cuts at: 220 220
Size: 4385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 1561 2440 2932 3230 164
Size: 1397 879 492 298 1319
Fragments arranged by size:
1397 1319 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4089 4089
Size: 4385
BsaAI yAC'GTr
Cuts at: 810 1738 810
Size: 928 3457
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 112 127 1816 2064 2766 4262 112
Size: 15 1689 248 702 1496 235
Fragments arranged by size:
1689 1496 702 248 235 15
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3047 3194 3605 1574
Size: 521 678 274 147 411 2354
Fragments arranged by size:
2354 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 199
Size: 4385
BseYI C'CCAG_C
Cuts at: 1810 2568 3145 3641 1810
Size: 758 577 496 2554
Fragments arranged by size:
2554 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 341 777 184
Size: 157 436 3792
Fragments arranged by size:
3792 436 157
BsiEI CG_ry'CG
Cuts at: 104 1927 1973 3181 104
Size: 1823 46 1208 1308
Fragments arranged by size:
1823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1399 2177 2367 3159 1399
Size: 778 190 792 2625
Fragments arranged by size:
2625 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1399 2010 2103 2177 2367 2429 3159 1399
Size: 611 93 74 190 62 730 2625
Fragments arranged by size:
2625 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 3561 3561
Size: 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1435 1649 1951 2332 1435
Size: 214 302 381 3488
Fragments arranged by size:
3488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2677 2731 4215 4380 50
Size: 2627 54 1484 165 55
Fragments arranged by size:
2627 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 350 2297 350
Size: 1947 2438
BsrFI r'CCGG_y
Cuts at: 2383 2564 2773 2383
Size: 181 209 3995
Fragments arranged by size:
3995 209 181
BsrGI T'GTAC_A
Cuts at: 572 572
Size: 4385
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 1226 1762 1226
Size: 536 3849
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstYI r'GATC_y
Cuts at: 1 159 680 1410 1877 1900 2235 2481 3493
Size: 158 521 730 467 23 335 246 1012
Cuts at: 3493 3579 3591 3937 1
Size: 86 12 346 449
Fragments arranged by size:
1012 730 521 467 449 346 335 246
158 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 703 965 703
Size: 262 4123
BtgI C'CryG_G
Cuts at: 107 1376 107
Size: 1269 3116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1100 2335 2516 2679 1100
Size: 1235 181 163 2806
Fragments arranged by size:
2806 1235 181 163
BtsI GCAGTG_nn'
Cuts at: 765 1628 765
Size: 863 3522
DraI TTT'AAA
Cuts at: 1299 1384 1299
Size: 85 4300
DraIII CAC_nnn'GTG
Cuts at: 1007 1007
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 2091 2949 2091
Size: 858 3527
EaeI y'GGCC_r
Cuts at: 1970 2144 2535 2562 3907 1970
Size: 174 391 27 1345 2448
Fragments arranged by size:
2448 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 1009 1048 1267 2408 2618 1009
Size: 39 219 1141 210 2776
Fragments arranged by size:
2776 1141 219 210 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 2903 3049 4195 2593
Size: 310 146 1146 2783
Fragments arranged by size:
2783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 585 1005 2210 2642 3389 3657 431
Size: 154 420 1205 432 747 268 1159
Fragments arranged by size:
1205 1159 747 432 420 268 154
EcoO109I rG'GnC_Cy
Cuts at: 215 985 1208 1524 215
Size: 770 223 316 3076
Fragments arranged by size:
3076 770 316 223
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1259 2404 1259
Size: 1145 3240
FspI TGC'GCA
Cuts at: 2166 2166
Size: 4385
HaeII r_GCGC'y
Cuts at: 1819 2067 3089 4068 1819
Size: 248 1022 979 2136
Fragments arranged by size:
2136 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 672 704 1054 1086 2227 2257 2259 2289 672
Size: 32 350 32 1141 30 2 30 2768
Fragments arranged by size:
2768 1141 350 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
KasI G'GCGC_C
Cuts at: 1815 2063 1815
Size: 248 4137
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 381 3056 3240 381
Size: 2675 184 1526
Fragments arranged by size:
2675 1526 184
MscI TGG'CCA
Cuts at: 2146 2146
Size: 4385
MslI CAynn'nnrTG
Cuts at: 662 728 938 2374 2501 662
Size: 66 210 1436 127 2546
Fragments arranged by size:
2546 1436 210 127 66
MspA1I CmG'CkG
Cuts at: 1496 1810 2170 3183 3428 1496
Size: 314 360 1013 245 2453
Fragments arranged by size:
2453 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 1816 2064 1816
Size: 248 4137
NcoI C'CATG_G
Cuts at: 107 107
Size: 4385
NdeI CA'TA_TG
Cuts at: 838 838
Size: 4385
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NheI G'CTAG_C
Cuts at: 1284 1284
Size: 4385
NspI r_CATG'y
Cuts at: 833 1229 1265 1284 1438 1938 2845 833
Size: 396 36 19 154 500 907 2373
Fragments arranged by size:
2373 907 500 396 154 36 19
PasI CC'CwG_GG
Cuts at: 138 551 138
Size: 413 3972
PciI A'CATG_T
Cuts at: 829 2841 829
Size: 2012 2373
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PpuMI rG'GwC_Cy
Cuts at: 215 215
Size: 4385
PsiI TTA'TAA
Cuts at: 743 743
Size: 4385
PstI C_TGCA'G
Cuts at: 1084 1432 2117 1084
Size: 348 685 3352
Fragments arranged by size:
3352 685 348
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 1810 2170 1810
Size: 360 4025
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 1009 1048 1267 2408 2618 1009
Size: 39 219 1141 210 2776
Fragments arranged by size:
2776 1141 219 210 39
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 1080 1428 2113 3106 3297 33
Size: 1047 348 685 993 191 1121
Fragments arranged by size:
1121 1047 993 685 348 191
SfoI GGC'GCC
Cuts at: 1817 2065 1817
Size: 248 4137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 179 1540 2455 2947 3209 179
Size: 1361 915 492 262 1355
Fragments arranged by size:
1361 1355 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 1265 1438 1938 1265
Size: 173 500 3712
Fragments arranged by size:
3712 500 173
SspI AAT'ATT
Cuts at: 422 511 422
Size: 89 4296
StyI C'CwwG_G
Cuts at: 107 1519 1873 107
Size: 1412 354 2619
Fragments arranged by size:
2619 1412 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1968 2101 2560 2769 1968
Size: 133 459 209 3584
Fragments arranged by size:
3584 459 209 133
TatI w'GTAC_w
Cuts at: 572 3900 572
Size: 3328 1057
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 350 382 3902 3934 350
Size: 32 3520 32 801
Fragments arranged by size:
3520 801 32 32
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1880 1880
Size: 4385
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
XmnI GAAnn'nnTTC
Cuts at: 1018 1321 1018
Size: 303 4082
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NspI PasI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BcgI BfrBI BglI BmgBI
BpmI BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI