Restriction map for
pF1KE0734
(Circular) (Six-base) MAPSORT of: pF1KE0734.seq Check: 7217 from: 1 to: 4379
pF1KE0734 4379 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 388 388
Size: 4379
AccI GT'mk_AC
Cuts at: 627 1417 627
Size: 790 3589
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4379
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 2204 2636 3383 3651 222
Size: 1982 432 747 268 950
Fragments arranged by size:
1982 950 747 432 268
AflIII A'CryG_T
Cuts at: 1731 2835 3812 1731
Size: 1104 977 2298
Fragments arranged by size:
2298 1104 977
AgeI A'CCGG_T
Cuts at: 2767 2767
Size: 4379
AlwNI CAG_nnn'CTG
Cuts at: 1684 3251 1684
Size: 1567 2812
ApaLI G'TGCA_C
Cuts at: 173 571 3149 173
Size: 398 2578 1403
Fragments arranged by size:
2578 1403 398
ApoI r'AATT_y
Cuts at: 1383 3732 3988 1383
Size: 2349 256 1774
Fragments arranged by size:
2349 1774 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4379
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 1157 1399 1157
Size: 242 4137
AvrII C'CTAG_G
Cuts at: 816 816
Size: 4379
BamHI G'GATC_C
Cuts at: 1404 1404
Size: 4379
BanI G'GyrC_C
Cuts at: 1395 1809 2057 2092 1395
Size: 414 248 35 3682
Fragments arranged by size:
3682 414 248 35
BanII G_rGCy'C
Cuts at: 281 467 1266 1393 2423 281
Size: 186 799 127 1030 2237
Fragments arranged by size:
2237 1030 799 186 127
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4131
BbsI GAAGACnn'nnnn_
Cuts at: 529 1360 529
Size: 831 3548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1186 1220 1186
Size: 34 4345
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3038 4379 2277
Size: 761 1341 2277
Fragments arranged by size:
2277 1341 761
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BglII A'GATC_T
Cuts at: 1894 3931 1894
Size: 2037 2342
BlpI GC'TnA_GC
Cuts at: 1361 1491 1361
Size: 130 4249
Bme1580I G_kGCm'C
Cuts at: 177 575 2004 2097 3153 177
Size: 398 1429 93 1056 1403
Fragments arranged by size:
1429 1403 1056 398 93
BmrI ACTGGGnnnn_n'
Cuts at: 1591 1672 2007 4151 1591
Size: 81 335 2144 1819
Fragments arranged by size:
2144 1819 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 868 2746 868
Size: 1878 2501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1097 481
Size: 616 3763
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1555 2434 2926 3224 853
Size: 702 879 492 298 2008
Fragments arranged by size:
2008 879 702 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 412 1092 4083 412
Size: 680 2991 708
Fragments arranged by size:
2991 708 680
BsaAI yAC'GTr
Cuts at: 346 570 1131 1732 346
Size: 224 561 601 2993
Fragments arranged by size:
2993 601 561 224
BsaBI GATnn'nnATC
Cuts at: 6 541 1893 3930 6
Size: 535 1352 2037 455
Fragments arranged by size:
2037 1352 535 455
BsaHI Gr'CG_yC
Cuts at: 1810 2058 2760 4256 1810
Size: 248 702 1496 1933
Fragments arranged by size:
1933 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1568 2089 2767 3041 3188 3599 1568
Size: 521 678 274 147 411 2348
Fragments arranged by size:
2348 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 475 505 475
Size: 30 4349
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 189 973 189
Size: 784 3595
BseYI C'CCAG_C
Cuts at: 1804 2562 3139 3635 1804
Size: 758 577 496 2548
Fragments arranged by size:
2548 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 416
Size: 4379
BsiEI CG_ry'CG
Cuts at: 104 1921 1967 3175 104
Size: 1817 46 1208 1308
Fragments arranged by size:
1817 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 177 281 351 467 575 1393 2171 2361 3153
Size: 104 70 116 108 818 778 190 792
Cuts at: 3153 177
Size: 1403
Fragments arranged by size:
1403 818 792 778 190 116 108 104
70
Bsp1286I G_dGCh'C
Cuts at: 177 281 351 467 575 1266 1393 2004 2097
Size: 104 70 116 108 691 127 611 93
Cuts at: 2097 2171 2361 2423 3153 177
Size: 74 190 62 730 1403
Fragments arranged by size:
1403 730 691 611 190 127 116 108
104 93 74 70 62
BspEI T'CCGG_A
Cuts at: 3599 3599
Size: 4379
BspHI T'CATG_A
Cuts at: 3555 3555
Size: 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 388 1429 1643 1945 2326 388
Size: 1041 214 302 381 2441
Fragments arranged by size:
2441 1041 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4209 4374 50
Size: 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2291 2291
Size: 4379
BsrFI r'CCGG_y
Cuts at: 2377 2558 2767 2377
Size: 181 209 3989
Fragments arranged by size:
3989 209 181
BssSI C'ACGA_G
Cuts at: 2650 3008 2650
Size: 358 4021
BstAPI GCAn_nnn'nTGC
Cuts at: 1756 1756
Size: 4379
BstBI TT'CG_AA
Cuts at: 1206 2740 1206
Size: 1534 2845
BstXI CCAn_nnnn'nTGG
Cuts at: 459 459
Size: 4379
BstYI r'GATC_y
Cuts at: 1 1404 1871 1894 2229 2475 3487 3573 3585
Size: 1403 467 23 335 246 1012 86 12
Cuts at: 3585 3931 1
Size: 346 449
Fragments arranged by size:
1403 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 158 452 1256 107
Size: 51 294 804 3230
Fragments arranged by size:
3230 804 294 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2329 2510 2673 2329
Size: 181 163 4035
Fragments arranged by size:
4035 181 163
BtsI GCAGTG_nn'
Cuts at: 177 399 1622 177
Size: 222 1223 2934
Fragments arranged by size:
2934 1223 222
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4379
DrdI GACnn_nn'nnGTC
Cuts at: 2085 2943 2085
Size: 858 3521
EaeI y'GGCC_r
Cuts at: 677 1183 1964 2138 2529 2556 3901 677
Size: 506 781 174 391 27 1345 1155
Fragments arranged by size:
1345 1155 781 506 391 174 27
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4379
EarI CTCTTCn'nnn_
Cuts at: 1042 1208 2402 2612 1042
Size: 166 1194 210 2809
Fragments arranged by size:
2809 1194 210 166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 2897 3043 4189 2587
Size: 310 146 1146 2777
Fragments arranged by size:
2777 1146 310 146
EcoICRI GAG'CTC
Cuts at: 279 465 1391 279
Size: 186 926 3267
Fragments arranged by size:
3267 926 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 481 1097 2204 2636 3383 3651 222
Size: 259 616 1107 432 747 268 950
Fragments arranged by size:
1107 950 747 616 432 268 259
EcoO109I rG'GnC_Cy
Cuts at: 229 1518 229
Size: 1289 3090
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4379
EcoRV GAT'ATC
Cuts at: 895 895
Size: 4379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 1368 1813 2061 3083 4062 1368
Size: 445 248 1022 979 1685
Fragments arranged by size:
1685 1022 979 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1171 1203 2221 2251 2253 2283 1171
Size: 32 1018 30 2 30 3267
Fragments arranged by size:
3267 1018 32 30 30 2
HincII GTy'rAC
Cuts at: 628 1418 628
Size: 790 3589
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4131
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4379
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3050 3234 3050
Size: 184 4195
MscI TGG'CCA
Cuts at: 679 2140 679
Size: 1461 2918
MslI CAynn'nnrTG
Cuts at: 1271 2368 2495 1271
Size: 1097 127 3155
Fragments arranged by size:
3155 1097 127
MspA1I CmG'CkG
Cuts at: 549 1490 1804 2164 3177 3422 549
Size: 941 314 360 1013 245 1506
Fragments arranged by size:
1506 1013 941 360 314 245
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4379
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4131
NcoI C'CATG_G
Cuts at: 107 158 452 107
Size: 51 294 4034
Fragments arranged by size:
4034 294 51
NdeI CA'TA_TG
Cuts at: 824 824
Size: 4379
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4379
NspI r_CATG'y
Cuts at: 652 1432 1932 2839 652
Size: 780 500 907 2192
Fragments arranged by size:
2192 907 780 500
PciI A'CATG_T
Cuts at: 2835 2835
Size: 4379
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PmlI CAC'GTG
Cuts at: 346 346
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 4347
PpuMI rG'GwC_Cy
Cuts at: 229 229
Size: 4379
PsiI TTA'TAA
Cuts at: 380 380
Size: 4379
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 574 606 1109 1141 574
Size: 32 503 32 3812
Fragments arranged by size:
3812 503 32 32
PstI C_TGCA'G
Cuts at: 1426 2111 1426
Size: 685 3694
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 549 1804 2164 549
Size: 1255 360 2764
Fragments arranged by size:
2764 1255 360
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 281 467 1393 281
Size: 186 926 3267
Fragments arranged by size:
3267 926 186
SalI G'TCGA_C
Cuts at: 626 1416 626
Size: 790 3589
SanDI GG'GwC_CC
Cuts at: 229 229
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 3896 3896
Size: 4379
SfcI C'TryA_G
Cuts at: 33 1422 2107 3100 3291 33
Size: 1389 685 993 191 1121
Fragments arranged by size:
1389 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4131
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 868 1157 1534 2449 2941 3203 868
Size: 289 377 915 492 262 2044
Fragments arranged by size:
2044 915 492 377 289 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3879
StyI C'CwwG_G
Cuts at: 107 158 452 468 680 816 1513 1867 107
Size: 51 294 16 212 136 697 354 2619
Fragments arranged by size:
2619 697 354 294 212 136 51 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 521 1962 2095 2554 2763 521
Size: 1441 133 459 209 2137
Fragments arranged by size:
2137 1441 459 209 133
TatI w'GTAC_w
Cuts at: 368 3894 368
Size: 3526 853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1306 1338 3896 3928 1306
Size: 32 2558 32 1757
Fragments arranged by size:
2558 1757 32 32
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4379
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1874 1874
Size: 4379
XhoI C'TCGA_G
Cuts at: 1157 1157
Size: 4379
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PmlI PpiI
PpuMI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PshAI PspOMI PspXI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI