Restriction map for
pF1KE0733
(Circular) (Six-base) MAPSORT of: pF1KE0733.seq Check: 576 from: 1 to: 4046
pF1KE0733 4046 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 373 373
Size: 4046
AccI GT'mk_AC
Cuts at: 1084 1084
Size: 4046
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1871 2303 3050 3318 1871
Size: 432 747 268 2599
Fragments arranged by size:
2599 747 432 268
AflIII A'CryG_T
Cuts at: 1398 2502 3479 1398
Size: 1104 977 1965
Fragments arranged by size:
1965 1104 977
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AlwNI CAG_nnn'CTG
Cuts at: 1351 2918 1351
Size: 1567 2479
ApaI G_GGCC'C
Cuts at: 508 508
Size: 4046
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 1050 3399 3655 1050
Size: 2349 256 1441
Fragments arranged by size:
2349 1441 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 145 454 1066 145
Size: 309 612 3125
Fragments arranged by size:
3125 612 309
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 730 763 730
Size: 33 4013
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 238 392 664 902 1062 1476 1724 1759 238
Size: 154 272 238 160 414 248 35 2525
Fragments arranged by size:
2525 414 272 248 238 160 154 35
BanII G_rGCy'C
Cuts at: 508 854 1060 2090 508
Size: 346 206 1030 2464
Fragments arranged by size:
2464 1030 346 206
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BbsI GAAGACnn'nnnn_
Cuts at: 237 237
Size: 4046
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 228 262 228
Size: 34 4012
BciVI GTATCCnnnnn_n'
Cuts at: 1944 2705 4046 1944
Size: 761 1341 1944
Fragments arranged by size:
1944 1341 761
BclI T'GATC_A
Cuts at: 1566 1566
Size: 4046
BglI GCCn_nnn'nGGC
Cuts at: 1021 1021
Size: 4046
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 848 1158 848
Size: 310 3736
Bme1580I G_kGCm'C
Cuts at: 395 508 669 891 1671 1764 2820 395
Size: 113 161 222 780 93 1056 1621
Fragments arranged by size:
1621 1056 780 222 161 113 93
BmrI ACTGGGnnnn_n'
Cuts at: 520 787 1258 1339 1674 3818 520
Size: 267 471 81 335 2144 748
Fragments arranged by size:
2144 748 471 335 267 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 750 2413 750
Size: 1663 2383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 259
Size: 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2101 2593 2891 1222
Size: 879 492 298 2377
Fragments arranged by size:
2377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3750 3750
Size: 4046
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 1477 1725 2427 3923 1477
Size: 248 702 1496 1600
Fragments arranged by size:
1600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 778 778
Size: 4046
BseYI C'CCAG_C
Cuts at: 312 861 1471 2229 2806 3302 312
Size: 549 610 758 577 496 1056
Fragments arranged by size:
1056 758 610 577 549 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 401
Size: 4046
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 854 1060 1838 2028 2820 854
Size: 206 778 190 792 2080
Fragments arranged by size:
2080 792 778 206 190
Bsp1286I G_dGCh'C
Cuts at: 395 508 669 854 891 1060 1671 1764 1838
Size: 113 161 185 37 169 611 93 74
Cuts at: 1838 2028 2090 2820 395
Size: 190 62 730 1621
Fragments arranged by size:
1621 730 611 190 185 169 161 113
93 74 62 37
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 675 3222 675
Size: 2547 1499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 373 1096 1310 1612 1993 373
Size: 723 214 302 381 2426
Fragments arranged by size:
2426 723 381 302 214
BsrBI CCG'CTC
Cuts at: 50 459 2338 2392 3876 4041 50
Size: 409 1879 54 1484 165 55
Fragments arranged by size:
1879 1484 409 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1958 1958
Size: 4046
BsrFI r'CCGG_y
Cuts at: 899 2044 2225 2434 899
Size: 1145 181 209 2511
Fragments arranged by size:
2511 1145 209 181
BsrGI T'GTAC_A
Cuts at: 713 713
Size: 4046
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstXI CCAn_nnnn'nTGG
Cuts at: 577 577
Size: 4046
BstYI r'GATC_y
Cuts at: 1 477 731 944 1071 1538 1561 1896 2142
Size: 476 254 213 127 467 23 335 246
Cuts at: 2142 3154 3240 3252 3598 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 476 467 449 346 335 254 246
213 127 86 23 12
BtgI C'CryG_G
Cuts at: 576 630 576
Size: 54 3992
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 361 564 1289 361
Size: 203 725 3118
Fragments arranged by size:
3118 725 203
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DraIII CAC_nnn'GTG
Cuts at: 514 514
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 281 540 761 1631 1805 2196 2223 3568 281
Size: 259 221 870 174 391 27 1345 759
Fragments arranged by size:
1345 870 759 391 259 221 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 935 2069 2279 935
Size: 1134 210 2702
Fragments arranged by size:
2702 1134 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 397 886 2254 2564 2710 3856 397
Size: 489 1368 310 146 1146 587
Fragments arranged by size:
1368 1146 587 489 310 146
EcoICRI GAG'CTC
Cuts at: 852 1058 852
Size: 206 3840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1871 2303 3050 3318 259
Size: 1612 432 747 268 987
Fragments arranged by size:
1612 987 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 223 822 223
Size: 599 3447
EcoO109I rG'GnC_Cy
Cuts at: 130 149 504 1009 1185 130
Size: 19 355 505 176 2991
Fragments arranged by size:
2991 505 355 176 19
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 249 2065 249
Size: 1816 2230
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 1480 1728 2750 3729 1480
Size: 248 1022 979 1797
Fragments arranged by size:
1797 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 245 277 1888 1918 1920 1950 105
Size: 32 108 32 1611 30 2 30 2201
Fragments arranged by size:
2201 1611 108 32 32 30 30 2
HincII GTy'rAC
Cuts at: 113 1085 113
Size: 972 3074
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2717 2901 2717
Size: 184 3862
MscI TGG'CCA
Cuts at: 283 542 1807 283
Size: 259 1265 2522
Fragments arranged by size:
2522 1265 259
MslI CAynn'nnrTG
Cuts at: 289 366 575 2035 2162 289
Size: 77 209 1460 127 2173
Fragments arranged by size:
2173 1460 209 127 77
MspA1I CmG'CkG
Cuts at: 530 1157 1471 1831 2844 3089 530
Size: 627 314 360 1013 245 1487
Fragments arranged by size:
1487 1013 627 360 314 245
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NspI r_CATG'y
Cuts at: 347 1099 1599 2506 347
Size: 752 500 907 1887
Fragments arranged by size:
1887 907 752 500
PciI A'CATG_T
Cuts at: 2502 2502
Size: 4046
PflMI CCAn_nnn'nTGG
Cuts at: 549 770 549
Size: 221 3825
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PpuMI rG'GwC_Cy
Cuts at: 149 149
Size: 4046
PspOMI G'GGCC_C
Cuts at: 504 504
Size: 4046
PspXI vC'TCGA_Gb
Cuts at: 454 454
Size: 4046
PstI C_TGCA'G
Cuts at: 1093 1778 1093
Size: 685 3361
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 1471 1831 1471
Size: 360 3686
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 854 1060 854
Size: 206 3840
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SanDI GG'GwC_CC
Cuts at: 149 149
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 124 1089 1774 2767 2958 33
Size: 91 965 685 993 191 1121
Fragments arranged by size:
1121 993 965 685 191 91
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SgrAI Cr'CCGG_yG
Cuts at: 899 899
Size: 4046
SmaI CCC'GGG
Cuts at: 147 1068 147
Size: 921 3125
SmlI C'TyrA_G
Cuts at: 454 1201 2116 2608 2870 454
Size: 747 915 492 262 1630
Fragments arranged by size:
1630 915 747 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
StyI C'CwwG_G
Cuts at: 1180 1534 1180
Size: 354 3692
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 367 502 1629 1762 2221 2430 367
Size: 135 1127 133 459 209 1983
Fragments arranged by size:
1983 1127 459 209 135 133
TatI w'GTAC_w
Cuts at: 713 3561 713
Size: 2848 1198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 363 395 556 588 767 799 3563 3595 363
Size: 32 161 32 179 32 2764 32 814
Fragments arranged by size:
2764 814 179 161 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 337 1843 337
Size: 1506 2540
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1541 1541
Size: 4046
XhoI C'TCGA_G
Cuts at: 454 454
Size: 4046
XmaI C'CCGG_G
Cuts at: 145 1066 145
Size: 921 3125
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PsrI SacII SexAI SfiI SnaBI SrfI
SspI StuI SwaI XmnI ZraI