Restriction map for
pF1KE0729
(Circular) (Six-base) MAPSORT of: pF1KE0729.seq Check: 8144 from: 1 to: 4040
pF1KE0729 4040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 991 1865 2297 3044 3312 340
Size: 651 874 432 747 268 1068
Fragments arranged by size:
1068 874 747 651 432 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 802 802
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 969 1345 2912 969
Size: 376 1567 2097
Fragments arranged by size:
2097 1567 376
ApaI G_GGCC'C
Cuts at: 906 906
Size: 4040
ApaLI G'TGCA_C
Cuts at: 673 2810 673
Size: 2137 1903
ApoI r'AATT_y
Cuts at: 265 1044 3393 3649 265
Size: 779 2349 256 656
Fragments arranged by size:
2349 779 656 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 905 1060 905
Size: 155 3885
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 215 1056 1470 1718 1753 215
Size: 841 414 248 35 2502
Fragments arranged by size:
2502 841 414 248 35
BanII G_rGCy'C
Cuts at: 115 491 906 1054 2084 115
Size: 376 415 148 1030 2071
Fragments arranged by size:
2071 1030 415 376 148
BbeI G_GCGC'C
Cuts at: 219 1474 1722 219
Size: 1255 248 2537
Fragments arranged by size:
2537 1255 248
BbsI GAAGACnn'nnnn_
Cuts at: 703 703
Size: 4040
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 677 906 1665 1758 2814 677
Size: 229 759 93 1056 1903
Fragments arranged by size:
1903 1056 759 229 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 849 2407 849
Size: 1558 2482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 379
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 847 1216 2095 2587 2885 277
Size: 570 369 879 492 298 1432
Fragments arranged by size:
1432 879 570 492 369 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 216 246 1471 1719 2421 3917 216
Size: 30 1225 248 702 1496 339
Fragments arranged by size:
1496 1225 702 339 248 30
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 667 697 667
Size: 30 4010
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 565 705 565
Size: 140 3900
BseYI C'CCAG_C
Cuts at: 135 188 196 204 627 1465 2223 2800 3296
Size: 53 8 8 423 838 758 577 496
Cuts at: 3296 135
Size: 879
Fragments arranged by size:
879 838 758 577 496 423 53 8
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 4040
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 365 491 677 791 1023 1054 1832 2022
Size: 250 126 186 114 232 31 778 190
Cuts at: 2022 2814 115
Size: 792 1341
Fragments arranged by size:
1341 792 778 250 232 190 186 126
114 31
Bsp1286I G_dGCh'C
Cuts at: 115 365 491 677 791 906 1023 1054 1665
Size: 250 126 186 114 115 117 31 611
Cuts at: 1665 1758 1832 2022 2084 2814 115
Size: 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 250 190 186 126 117
115 114 93 74 62 31
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 159 629 1090 1304 1606 1987 159
Size: 470 461 214 302 381 2212
Fragments arranged by size:
2212 470 461 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 369 1417 369
Size: 1048 2992
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstXI CCAn_nnnn'nTGG
Cuts at: 126 126
Size: 4040
BstYI r'GATC_y
Cuts at: 1 386 965 1065 1532 1555 1890 2136 3148
Size: 385 579 100 467 23 335 246 1012
Cuts at: 3148 3234 3246 3592 1
Size: 86 12 346 449
Fragments arranged by size:
1012 579 467 449 385 346 335 246
100 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 688 688
Size: 4040
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1283
Size: 4040
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 1625 1799 2190 2217 3562 1625
Size: 174 391 27 1345 2103
Fragments arranged by size:
2103 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 155 334 2063 2273 155
Size: 179 1729 210 1922
Fragments arranged by size:
1922 1729 210 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 489 1052 113
Size: 376 563 3101
Fragments arranged by size:
3101 563 376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 379 991 1865 2297 3044 3312 340
Size: 39 612 874 432 747 268 1068
Fragments arranged by size:
1068 874 747 612 432 268 39
EcoNI CCTnn'n_nnAGG
Cuts at: 256 687 256
Size: 431 3609
EcoO109I rG'GnC_Cy
Cuts at: 902 1179 902
Size: 277 3763
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
EcoRV GAT'ATC
Cuts at: 814 814
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 345 2059 345
Size: 1714 2326
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 219 833 1474 1722 2744 3723 219
Size: 614 641 248 1022 979 536
Fragments arranged by size:
1022 979 641 614 536 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 378 410 845 877 1882 1912 1914 1944 378
Size: 32 435 32 1005 30 2 30 2474
Fragments arranged by size:
2474 1005 435 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 215 1470 1718 215
Size: 1255 248 2537
Fragments arranged by size:
2537 1255 248
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 784 2029 2156 784
Size: 1245 127 2668
Fragments arranged by size:
2668 1245 127
MspA1I CmG'CkG
Cuts at: 135 369 1151 1465 1825 2838 3083 135
Size: 234 782 314 360 1013 245 1092
Fragments arranged by size:
1092 1013 782 360 314 245 234
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 216 1471 1719 216
Size: 1255 248 2537
Fragments arranged by size:
2537 1255 248
NdeI CA'TA_TG
Cuts at: 753 753
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 1093 1593 2500 1093
Size: 500 907 2633
Fragments arranged by size:
2633 907 500
PasI CC'CwG_GG
Cuts at: 252 980 252
Size: 728 3312
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 942 942
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PspOMI G'GGCC_C
Cuts at: 902 902
Size: 4040
PstI C_TGCA'G
Cuts at: 156 506 1087 1772 156
Size: 350 581 685 2424
Fragments arranged by size:
2424 685 581 350
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 135 369 1465 1825 135
Size: 234 1096 360 2350
Fragments arranged by size:
2350 1096 360 234
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 115 491 1054 115
Size: 376 563 3101
Fragments arranged by size:
3101 563 376
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 156 1087 156
Size: 931 3109
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 152 170 502 1083 1768 2761 2952 33
Size: 119 18 332 581 685 993 191 1121
Fragments arranged by size:
1121 993 685 581 332 191 119 18
SfoI GGC'GCC
Cuts at: 217 1472 1720 217
Size: 1255 248 2537
Fragments arranged by size:
2537 1255 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 907 1062 907
Size: 155 3885
SmlI C'TyrA_G
Cuts at: 292 862 1195 2110 2602 2864 292
Size: 570 333 915 492 262 1468
Fragments arranged by size:
1468 915 570 492 333 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 478 941 1174 1528 478
Size: 463 233 354 2990
Fragments arranged by size:
2990 463 354 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 667 699 760 792 3557 3589 667
Size: 32 61 32 2765 32 1118
Fragments arranged by size:
2765 1118 61 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XmaI C'CCGG_G
Cuts at: 905 1060 905
Size: 155 3885
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
BtgI ClaI CspCI DraIII FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI