Restriction map for
pF1KE0726
(Circular) (Six-base) MAPSORT of: pF1KE0726.seq Check: 3012 from: 1 to: 4364
pF1KE0726 4364 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 441 441
Size: 4364
AccI GT'mk_AC
Cuts at: 916 1402 916
Size: 486 3878
Acc65I G'GTAC_C
Cuts at: 1380 1380
Size: 4364
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 2189 2621 3368 3636 335
Size: 1854 432 747 268 1063
Fragments arranged by size:
1854 1063 747 432 268
AflII C'TTAA_G
Cuts at: 1327 1327
Size: 4364
AflIII A'CryG_T
Cuts at: 562 1282 1716 2820 3797 562
Size: 720 434 1104 977 1129
Fragments arranged by size:
1129 1104 977 720 434
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AhdI GACnn_n'nnGTC
Cuts at: 713 713
Size: 4364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 538 538
Size: 4364
AlwNI CAG_nnn'CTG
Cuts at: 1669 3236 1669
Size: 1567 2797
ApaLI G'TGCA_C
Cuts at: 3134 3134
Size: 4364
ApoI r'AATT_y
Cuts at: 219 975 1079 1368 3717 3973 219
Size: 756 104 289 2349 256 610
Fragments arranged by size:
2349 756 610 289 256 104
AseI AT'TA_AT
Cuts at: 20 20
Size: 4364
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 1384 1384
Size: 4364
BamHI G'GATC_C
Cuts at: 808 1389 808
Size: 581 3783
BanI G'GyrC_C
Cuts at: 767 1380 1794 2042 2077 767
Size: 613 414 248 35 3054
Fragments arranged by size:
3054 613 414 248 35
BanII G_rGCy'C
Cuts at: 1378 2408 1378
Size: 1030 3334
BbeI G_GCGC'C
Cuts at: 1798 2046 1798
Size: 248 4116
BbsI GAAGACnn'nnnn_
Cuts at: 377 638 809 377
Size: 261 171 3932
Fragments arranged by size:
3932 261 171
BbvCI CC'TCA_GC
Cuts at: 281 281
Size: 4364
BciVI GTATCCnnnnn_n'
Cuts at: 565 2262 3023 4364 565
Size: 1697 761 1341 565
Fragments arranged by size:
1697 1341 761 565
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4364
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4364
Bme1580I G_kGCm'C
Cuts at: 822 1989 2082 3138 822
Size: 1167 93 1056 2048
Fragments arranged by size:
2048 1167 1056 93
BmgBI CAC'GTC
Cuts at: 138 207 138
Size: 69 4295
BmrI ACTGGGnnnn_n'
Cuts at: 959 1576 1657 1992 4136 959
Size: 617 81 335 2144 1187
Fragments arranged by size:
2144 1187 617 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 349 891 2731 349
Size: 542 1840 1982
Fragments arranged by size:
1982 1840 542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 861 1025 1109 1277 396
Size: 465 164 84 168 3483
Fragments arranged by size:
3483 465 168 164 84
Bpu10I CC'TnA_GC
Cuts at: 281 281
Size: 4364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 1540 2419 2911 3209 555
Size: 985 879 492 298 1710
Fragments arranged by size:
1710 985 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4068 4068
Size: 4364
BsaAI yAC'GTr
Cuts at: 565 739 1717 565
Size: 174 978 3212
Fragments arranged by size:
3212 978 174
BsaBI GATnn'nnATC
Cuts at: 6 1878 3915 6
Size: 1872 2037 455
Fragments arranged by size:
2037 1872 455
BsaHI Gr'CG_yC
Cuts at: 1795 2043 2745 4241 1795
Size: 248 702 1496 1918
Fragments arranged by size:
1918 1496 702 248
BsaWI w'CCGG_w
Cuts at: 979 1553 2074 2752 3026 3173 3584 979
Size: 574 521 678 274 147 411 1759
Fragments arranged by size:
1759 678 574 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 4364
BseYI C'CCAG_C
Cuts at: 180 350 1789 2547 3124 3620 180
Size: 170 1439 758 577 496 924
Fragments arranged by size:
1439 924 758 577 496 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 1195
Size: 4364
BsiEI CG_ry'CG
Cuts at: 104 1906 1952 3160 104
Size: 1802 46 1208 1308
Fragments arranged by size:
1802 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1378 2156 2346 3138 1378
Size: 778 190 792 2604
Fragments arranged by size:
2604 792 778 190
BsmI GAATG_Cn'
Cuts at: 235 235
Size: 4364
BsmBI CGTCTCn'nnnn_
Cuts at: 197 197
Size: 4364
Bsp1286I G_dGCh'C
Cuts at: 822 1378 1989 2082 2156 2346 2408 3138 822
Size: 556 611 93 74 190 62 730 2048
Fragments arranged by size:
2048 730 611 556 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3584 3584
Size: 4364
BspHI T'CATG_A
Cuts at: 483 3540 483
Size: 3057 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 441 520 1414 1628 1930 2311 441
Size: 79 894 214 302 381 2494
Fragments arranged by size:
2494 894 381 302 214 79
BsrBI CCG'CTC
Cuts at: 50 2656 2710 4194 4359 50
Size: 2606 54 1484 165 55
Fragments arranged by size:
2606 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 996 2276 996
Size: 1280 3084
BsrFI r'CCGG_y
Cuts at: 2362 2543 2752 2362
Size: 181 209 3974
Fragments arranged by size:
3974 209 181
BssSI C'ACGA_G
Cuts at: 336 2635 2993 336
Size: 2299 358 1707
Fragments arranged by size:
2299 1707 358
BstAPI GCAn_nnn'nTGC
Cuts at: 961 1173 1741 961
Size: 212 568 3584
Fragments arranged by size:
3584 568 212
BstBI TT'CG_AA
Cuts at: 2725 2725
Size: 4364
BstEII G'GTnAC_C
Cuts at: 123 123
Size: 4364
BstYI r'GATC_y
Cuts at: 1 808 1389 1856 1879 2214 2460 3472 3558
Size: 807 581 467 23 335 246 1012 86
Cuts at: 3558 3570 3916 1
Size: 12 346 449
Fragments arranged by size:
1012 807 581 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 917 917
Size: 4364
Bsu36I CC'TnA_GG
Cuts at: 814 814
Size: 4364
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2314 2495 2658 2314
Size: 181 163 4020
Fragments arranged by size:
4020 181 163
BtsI GCAGTG_nn'
Cuts at: 1607 1607
Size: 4364
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4364
DrdI GACnn_nn'nnGTC
Cuts at: 640 2070 2928 640
Size: 1430 858 2076
Fragments arranged by size:
2076 1430 858
EaeI y'GGCC_r
Cuts at: 1949 2123 2514 2541 3886 1949
Size: 174 391 27 1345 2427
Fragments arranged by size:
2427 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4364
EarI CTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2572 2882 3028 4174 2572
Size: 310 146 1146 2762
Fragments arranged by size:
2762 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1376 1376
Size: 4364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 396 861 1025 1109 1277 2189 2621 3368
Size: 61 465 164 84 168 912 432 747
Cuts at: 3368 3636 335
Size: 268 1063
Fragments arranged by size:
1063 912 747 465 432 268 168 164
84 61
EcoO109I rG'GnC_Cy
Cuts at: 1503 1503
Size: 4364
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4364
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 1798 2046 3068 4047 1798
Size: 248 1022 979 2115
Fragments arranged by size:
2115 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 539 571 679 699 711 731 2206 2236 2238
Size: 32 108 20 12 20 1475 30 2
Cuts at: 2238 2268 539
Size: 30 2635
Fragments arranged by size:
2635 1475 108 32 30 30 20 20
12 2
HincII GTy'rAC
Cuts at: 1403 1403
Size: 4364
HindIII A'AGCT_T
Cuts at: 791 791
Size: 4364
KasI G'GCGC_C
Cuts at: 1794 2042 1794
Size: 248 4116
KpnI G_GTAC'C
Cuts at: 1384 1384
Size: 4364
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3035 3219 3035
Size: 184 4180
MscI TGG'CCA
Cuts at: 2125 2125
Size: 4364
MslI CAynn'nnrTG
Cuts at: 737 904 1340 2353 2480 737
Size: 167 436 1013 127 2621
Fragments arranged by size:
2621 1013 436 167 127
MspA1I CmG'CkG
Cuts at: 774 1475 1789 2149 3162 3407 774
Size: 701 314 360 1013 245 1731
Fragments arranged by size:
1731 1013 701 360 314 245
NaeI GCC'GGC
Cuts at: 2545 2545
Size: 4364
NarI GG'CG_CC
Cuts at: 1795 2043 1795
Size: 248 4116
NdeI CA'TA_TG
Cuts at: 1073 1073
Size: 4364
NgoMIV G'CCGG_C
Cuts at: 2543 2543
Size: 4364
NspI r_CATG'y
Cuts at: 1171 1286 1417 1917 2824 1171
Size: 115 131 500 907 2711
Fragments arranged by size:
2711 907 500 131 115
PciI A'CATG_T
Cuts at: 1282 2820 1282
Size: 1538 2826
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PmlI CAC'GTG
Cuts at: 565 565
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 330 362 3503 3535 330
Size: 32 3141 32 1159
Fragments arranged by size:
3141 1159 32 32
PstI C_TGCA'G
Cuts at: 438 517 1411 2096 438
Size: 79 894 685 2706
Fragments arranged by size:
2706 894 685 79
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 1789 2149 1789
Size: 360 4004
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 1378 1378
Size: 4364
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
SbfI CC_TGCA'GG
Cuts at: 517 1411 517
Size: 894 3470
ScaI AGT'ACT
Cuts at: 3881 3881
Size: 4364
SfcI C'TryA_G
Cuts at: 33 198 434 513 1407 2092 3085 3276 33
Size: 165 236 79 894 685 993 191 1121
Fragments arranged by size:
1121 993 894 685 236 191 165 79
SfoI GGC'GCC
Cuts at: 1796 2044 1796
Size: 248 4116
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4364
SmlI C'TyrA_G
Cuts at: 570 1327 1519 2434 2926 3188 570
Size: 757 192 915 492 262 1746
Fragments arranged by size:
1746 915 757 492 262 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
StyI C'CwwG_G
Cuts at: 1498 1852 1498
Size: 354 4010
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1947 2080 2539 2748 1947
Size: 133 459 209 3563
Fragments arranged by size:
3563 459 209 133
TatI w'GTAC_w
Cuts at: 3879 3879
Size: 4364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4332
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4364
XbaI T'CTAG_A
Cuts at: 1395 1395
Size: 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1859 1859
Size: 4364
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4364
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AleI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsaXI BsiWI BsrGI BssHII
BstXI BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI