Restriction map for
pF1KE0724
(Circular) (Six-base) MAPSORT of: pF1KE0724.seq Check: 6944 from: 1 to: 4358
pF1KE0724 4358 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 422 1396 422
Size: 974 3384
Acc65I G'GTAC_C
Cuts at: 896 1374 896
Size: 478 3880
AclI AA'CG_TT
Cuts at: 4198 4198
Size: 4358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2615 3362 3630 2183
Size: 432 747 268 2911
Fragments arranged by size:
2911 747 432 268
AflIII A'CryG_T
Cuts at: 827 1710 2814 3791 827
Size: 883 1104 977 1394
Fragments arranged by size:
1394 1104 977 883
AgeI A'CCGG_T
Cuts at: 377 2746 377
Size: 2369 1989
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 364 396 478 510 364
Size: 32 82 32 4212
Fragments arranged by size:
4212 82 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1270 1663 3230 1270
Size: 393 1567 2398
Fragments arranged by size:
2398 1567 393
ApaI G_GGCC'C
Cuts at: 238 357 479 238
Size: 119 122 4117
Fragments arranged by size:
4117 122 119
ApaLI G'TGCA_C
Cuts at: 574 3128 574
Size: 2554 1804
ApoI r'AATT_y
Cuts at: 1362 3711 3967 1362
Size: 2349 256 1753
Fragments arranged by size:
2349 1753 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
AvaI C'yCGr_G
Cuts at: 204 216 350 610 633 700 1378 204
Size: 12 134 260 23 67 678 3184
Fragments arranged by size:
3184 678 260 134 67 23 12
AvrII C'CTAG_G
Cuts at: 291 291
Size: 4358
BamHI G'GATC_C
Cuts at: 1383 1383
Size: 4358
BanI G'GyrC_C
Cuts at: 896 992 1123 1374 1788 2036 2071 896
Size: 96 131 251 414 248 35 3183
Fragments arranged by size:
3183 414 251 248 131 96 35
BanII G_rGCy'C
Cuts at: 205 238 287 357 479 872 1372 2402 205
Size: 33 49 70 122 393 500 1030 2161
Fragments arranged by size:
2161 1030 500 393 122 70 49 33
BbeI G_GCGC'C
Cuts at: 996 1792 2040 996
Size: 796 248 3314
Fragments arranged by size:
3314 796 248
BbvCI CC'TCA_GC
Cuts at: 1154 1154
Size: 4358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 243 277 1050 1084 243
Size: 34 773 34 3517
Fragments arranged by size:
3517 773 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2256 3017 4358 2256
Size: 761 1341 2256
Fragments arranged by size:
2256 1341 761
BclI T'GATC_A
Cuts at: 554 1878 554
Size: 1324 3034
BglI GCCn_nnn'nGGC
Cuts at: 746 746
Size: 4358
BglII A'GATC_T
Cuts at: 1873 3910 1873
Size: 2037 2321
BlpI GC'TnA_GC
Cuts at: 394 1470 394
Size: 1076 3282
Bme1580I G_kGCm'C
Cuts at: 238 357 479 578 751 1983 2076 3132 238
Size: 119 122 99 173 1232 93 1056 1464
Fragments arranged by size:
1464 1232 1056 173 122 119 99 93
BmgBI CAC'GTC
Cuts at: 603 603
Size: 4358
BmrI ACTGGGnnnn_n'
Cuts at: 1101 1570 1651 1986 4130 1101
Size: 469 81 335 2144 1329
Fragments arranged by size:
2144 1329 469 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 4358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 484 505 832 391
Size: 93 21 327 3917
Fragments arranged by size:
3917 327 93 21
Bpu10I CC'TnA_GC
Cuts at: 1154 1154
Size: 4358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 856 871 1534 2413 2905 3203 508
Size: 348 15 663 879 492 298 1663
Fragments arranged by size:
1663 879 663 492 348 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 697 4062 697
Size: 3365 993
BsaAI yAC'GTr
Cuts at: 321 974 1711 321
Size: 653 737 2968
Fragments arranged by size:
2968 737 653
BsaBI GATnn'nnATC
Cuts at: 6 544 1872 3909 6
Size: 538 1328 2037 455
Fragments arranged by size:
2037 1328 538 455
BsaHI Gr'CG_yC
Cuts at: 410 993 1789 2037 2739 4235 410
Size: 583 796 248 702 1496 533
Fragments arranged by size:
1496 796 702 583 533 248
BsaWI w'CCGG_w
Cuts at: 197 377 509 1547 2068 2746 3020 3167 3578
Size: 180 132 1038 521 678 274 147 411
Cuts at: 3578 197
Size: 977
Fragments arranged by size:
1038 977 678 521 411 274 180 147
132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 364 394 478 508 1129 1159 364
Size: 30 84 30 621 30 3563
Fragments arranged by size:
3563 621 84 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 976 1335 160
Size: 816 359 3183
Fragments arranged by size:
3183 816 359
BseYI C'CCAG_C
Cuts at: 471 478 819 1148 1172 1783 2541 3118 3614
Size: 7 341 329 24 611 758 577 496
Cuts at: 3614 471
Size: 1215
Fragments arranged by size:
1215 758 611 577 496 341 329 24
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 4358
BsiEI CG_ry'CG
Cuts at: 104 510 1059 1241 1900 1946 3154 104
Size: 406 549 182 659 46 1208 1308
Fragments arranged by size:
1308 1208 659 549 406 182 46
BsiHKAI G_wGCw'C
Cuts at: 205 287 578 734 944 1372 2150 2340 3132
Size: 82 291 156 210 428 778 190 792
Cuts at: 3132 205
Size: 1431
Fragments arranged by size:
1431 792 778 428 291 210 190 156
82
BsmI GAATG_Cn'
Cuts at: 1227 1227
Size: 4358
BsmBI CGTCTCn'nnnn_
Cuts at: 133 133
Size: 4358
Bsp1286I G_dGCh'C
Cuts at: 205 238 287 357 479 578 734 751 872
Size: 33 49 70 122 99 156 17 121
Cuts at: 872 944 1372 1983 2076 2150 2340 2402 3132
Size: 72 428 611 93 74 190 62 730
Cuts at: 3132 205
Size: 1431
Fragments arranged by size:
1431 730 611 428 190 156 122 121
99 93 74 72 70 62 49 33
17
BspEI T'CCGG_A
Cuts at: 197 3578 197
Size: 3381 977
BspHI T'CATG_A
Cuts at: 3534 3534
Size: 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 986 1037 1148 1408 1622 1924 2305 986
Size: 51 111 260 214 302 381 3039
Fragments arranged by size:
3039 381 302 260 214 111 51
BsrBI CCG'CTC
Cuts at: 50 894 2650 2704 4188 4353 50
Size: 844 1756 54 1484 165 55
Fragments arranged by size:
1756 1484 844 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2270 2270
Size: 4358
BsrFI r'CCGG_y
Cuts at: 169 377 1240 2356 2537 2746 169
Size: 208 863 1116 181 209 1781
Fragments arranged by size:
1781 1116 863 209 208 181
BssHII G'CGCG_C
Cuts at: 754 754
Size: 4358
BssSI C'ACGA_G
Cuts at: 2629 2987 2629
Size: 358 4000
BstAPI GCAn_nnn'nTGC
Cuts at: 1735 1735
Size: 4358
BstBI TT'CG_AA
Cuts at: 2719 2719
Size: 4358
BstXI CCAn_nnnn'nTGG
Cuts at: 462 462
Size: 4358
BstYI r'GATC_y
Cuts at: 1 1383 1850 1873 2208 2454 3466 3552 3564
Size: 1382 467 23 335 246 1012 86 12
Cuts at: 3564 3910 1
Size: 346 449
Fragments arranged by size:
1382 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 416 416
Size: 4358
BtgI C'CryG_G
Cuts at: 107 189 455 1235 107
Size: 82 266 780 3230
Fragments arranged by size:
3230 780 266 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2308 2489 2652 2308
Size: 181 163 4014
Fragments arranged by size:
4014 181 163
BtsI GCAGTG_nn'
Cuts at: 1068 1601 1068
Size: 533 3825
DraI TTT'AAA
Cuts at: 1357 1357
Size: 4358
DrdI GACnn_nn'nnGTC
Cuts at: 2064 2922 2064
Size: 858 3500
EaeI y'GGCC_r
Cuts at: 680 968 1238 1242 1286 1943 2117 2508 2535
Size: 288 270 4 44 657 174 391 27
Cuts at: 2535 3880 680
Size: 1345 1158
Fragments arranged by size:
1345 1158 657 391 288 270 174 44
27 4
EagI C'GGCC_G
Cuts at: 1238 1943 1238
Size: 705 3653
EarI CTCTTCn'nnn_
Cuts at: 1045 1057 2381 2591 1045
Size: 12 1324 210 2812
Fragments arranged by size:
2812 1324 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 277 2566 2876 3022 4168 277
Size: 2289 310 146 1146 467
Fragments arranged by size:
2289 1146 467 310 146
EcoICRI GAG'CTC
Cuts at: 203 285 1370 203
Size: 82 1085 3191
Fragments arranged by size:
3191 1085 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 484 505 832 2183 2615 3362 3630 391
Size: 93 21 327 1351 432 747 268 1119
Fragments arranged by size:
1351 1119 747 432 327 268 93 21
EcoNI CCTnn'n_nnAGG
Cuts at: 784 784
Size: 4358
EcoO109I rG'GnC_Cy
Cuts at: 222 235 354 475 489 1497 222
Size: 13 119 121 14 1008 3083
Fragments arranged by size:
3083 1008 121 119 14 13
EcoRI G'AATT_C
Cuts at: 1362 1362
Size: 4358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4358
FseI GG_CCGG'CC
Cuts at: 1244 1244
Size: 4358
FspI TGC'GCA
Cuts at: 2139 2139
Size: 4358
HaeII r_GCGC'y
Cuts at: 401 820 996 1270 1792 2040 3062 4041 401
Size: 419 176 274 522 248 1022 979 718
Fragments arranged by size:
1022 979 718 522 419 274 248 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1129 1161 2200 2230 2232 2262 1129
Size: 32 1039 30 2 30 3225
Fragments arranged by size:
3225 1039 32 30 30 2
HincII GTy'rAC
Cuts at: 1397 1397
Size: 4358
KasI G'GCGC_C
Cuts at: 992 1788 2036 992
Size: 796 248 3314
Fragments arranged by size:
3314 796 248
KpnI G_GTAC'C
Cuts at: 900 1378 900
Size: 478 3880
MluI A'CGCG_T
Cuts at: 3791 3791
Size: 4358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1274 3029 3213 1274
Size: 1755 184 2419
Fragments arranged by size:
2419 1755 184
MscI TGG'CCA
Cuts at: 682 970 2119 682
Size: 288 1149 2921
Fragments arranged by size:
2921 1149 288
MslI CAynn'nnrTG
Cuts at: 562 832 937 2347 2474 562
Size: 270 105 1410 127 2446
Fragments arranged by size:
2446 1410 270 127 105
MspA1I CmG'CkG
Cuts at: 363 552 1469 1783 2143 3156 3401 363
Size: 189 917 314 360 1013 245 1320
Fragments arranged by size:
1320 1013 917 360 314 245 189
NaeI GCC'GGC
Cuts at: 171 1242 2539 171
Size: 1071 1297 1990
Fragments arranged by size:
1990 1297 1071
NarI GG'CG_CC
Cuts at: 993 1789 2037 993
Size: 796 248 3314
Fragments arranged by size:
3314 796 248
NcoI C'CATG_G
Cuts at: 107 455 107
Size: 348 4010
NgoMIV G'CCGG_C
Cuts at: 169 1240 2537 169
Size: 1071 1297 1990
Fragments arranged by size:
1990 1297 1071
NspI r_CATG'y
Cuts at: 655 831 1411 1911 2818 655
Size: 176 580 500 907 2195
Fragments arranged by size:
2195 907 580 500 176
PciI A'CATG_T
Cuts at: 827 2814 827
Size: 1987 2371
PfoI T'CCnGG_A
Cuts at: 1160 1160
Size: 4358
PmeI GTTT'AAAC
Cuts at: 1357 1357
Size: 4358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 364 396 478 510 3497 3529 364
Size: 32 82 32 2987 32 1193
Fragments arranged by size:
2987 1193 82 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 222 489 222
Size: 267 4091
PspOMI G'GGCC_C
Cuts at: 234 353 475 234
Size: 119 122 4117
Fragments arranged by size:
4117 122 119
PstI C_TGCA'G
Cuts at: 1275 1343 1405 2090 1275
Size: 68 62 685 3543
Fragments arranged by size:
3543 685 68 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4358
PvuII CAG'CTG
Cuts at: 363 552 1783 2143 363
Size: 189 1231 360 2578
Fragments arranged by size:
2578 1231 360 189
RsrII CG'GwC_CG
Cuts at: 433 2553 433
Size: 2120 2238
SacI G_AGCT'C
Cuts at: 205 287 1372 205
Size: 82 1085 3191
Fragments arranged by size:
3191 1085 82
SalI G'TCGA_C
Cuts at: 1395 1395
Size: 4358
SapI GCTCTTCn'nnn_
Cuts at: 1045 2381 2591 1045
Size: 1336 210 2812
Fragments arranged by size:
2812 1336 210
SbfI CC_TGCA'GG
Cuts at: 1405 1405
Size: 4358
ScaI AGT'ACT
Cuts at: 3875 3875
Size: 4358
SfcI C'TryA_G
Cuts at: 33 1271 1339 1401 2086 3079 3270 33
Size: 1238 68 62 685 993 191 1121
Fragments arranged by size:
1238 1121 993 685 191 68 62
SfoI GGC'GCC
Cuts at: 994 1790 2038 994
Size: 796 248 3314
Fragments arranged by size:
3314 796 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
SmaI CCC'GGG
Cuts at: 352 635 1380 352
Size: 283 745 3330
Fragments arranged by size:
3330 745 283
SmlI C'TyrA_G
Cuts at: 523 871 886 1513 2428 2920 3182 523
Size: 348 15 627 915 492 262 1699
Fragments arranged by size:
1699 915 627 492 348 262 15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4358
SphI G_CATG'C
Cuts at: 655 1411 1911 655
Size: 756 500 3102
Fragments arranged by size:
3102 756 500
StyI C'CwwG_G
Cuts at: 107 164 291 455 683 1492 1846 107
Size: 57 127 164 228 809 354 2619
Fragments arranged by size:
2619 809 354 228 164 127 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 1941 2074 2533 2742 421
Size: 1520 133 459 209 2037
Fragments arranged by size:
2037 1520 459 209 133
TatI w'GTAC_w
Cuts at: 446 689 836 3873 446
Size: 243 147 3037 931
Fragments arranged by size:
3037 931 243 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 4326
Tth111I GACn'n_nGTC
Cuts at: 2155 2155
Size: 4358
XbaI T'CTAG_A
Cuts at: 1389 1389
Size: 4358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1853 1853
Size: 4358
XmaI C'CCGG_G
Cuts at: 350 633 1378 350
Size: 283 745 3330
Fragments arranged by size:
3330 745 283
XmnI GAAnn'nnTTC
Cuts at: 1039 1039
Size: 4358
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
BaeI BbsI BfrBI BmtI BsiWI BsrGI BstEII BstZ17I
ClaI CspCI DraIII EcoRV FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI