Restriction map for
pF1KE0720
(Circular) (Six-base) MAPSORT of: pF1KE0720.seq Check: 1725 from: 1 to: 4355
pF1KE0720 4355 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1166 1336 1166
Size: 170 4185
AccI GT'mk_AC
Cuts at: 725 1393 725
Size: 668 3687
Acc65I G'GTAC_C
Cuts at: 524 1371 524
Size: 847 3508
AclI AA'CG_TT
Cuts at: 4195 4195
Size: 4355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 2180 2612 3359 3627 502
Size: 1678 432 747 268 1230
Fragments arranged by size:
1678 1230 747 432 268
AflIII A'CryG_T
Cuts at: 392 582 821 1707 2811 3788 392
Size: 190 239 886 1104 977 959
Fragments arranged by size:
1104 977 959 886 239 190
AgeI A'CCGG_T
Cuts at: 2743 2743
Size: 4355
AhdI GACnn_n'nnGTC
Cuts at: 732 732
Size: 4355
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 379 411 964 996 379
Size: 32 553 32 3738
Fragments arranged by size:
3738 553 32 32
AlwNI CAG_nnn'CTG
Cuts at: 183 1660 3227 183
Size: 1477 1567 1311
Fragments arranged by size:
1567 1477 1311
ApaLI G'TGCA_C
Cuts at: 3125 3125
Size: 4355
ApoI r'AATT_y
Cuts at: 1189 1359 3708 3964 1189
Size: 170 2349 256 1580
Fragments arranged by size:
2349 1580 256 170
AseI AT'TA_AT
Cuts at: 20 20
Size: 4355
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 1375 1375
Size: 4355
BamHI G'GATC_C
Cuts at: 903 1380 903
Size: 477 3878
BanI G'GyrC_C
Cuts at: 524 652 1371 1785 2033 2068 524
Size: 128 719 414 248 35 2811
Fragments arranged by size:
2811 719 414 248 128 35
BanII G_rGCy'C
Cuts at: 1369 2399 1369
Size: 1030 3325
BbeI G_GCGC'C
Cuts at: 1789 2037 1789
Size: 248 4107
BbvCI CC'TCA_GC
Cuts at: 883 883
Size: 4355
BciVI GTATCCnnnnn_n'
Cuts at: 703 2253 3014 4355 703
Size: 1550 761 1341 703
Fragments arranged by size:
1550 1341 761 703
BclI T'GATC_A
Cuts at: 1875 1875
Size: 4355
BfrBI ATG'CAT
Cuts at: 221 221
Size: 4355
BglII A'GATC_T
Cuts at: 1870 3907 1870
Size: 2037 2318
BlpI GC'TnA_GC
Cuts at: 1467 1467
Size: 4355
Bme1580I G_kGCm'C
Cuts at: 950 1013 1980 2073 3129 950
Size: 63 967 93 1056 2176
Fragments arranged by size:
2176 1056 967 93 63
BmrI ACTGGGnnnn_n'
Cuts at: 978 1567 1648 1983 4127 978
Size: 589 81 335 2144 1206
Fragments arranged by size:
2144 1206 589 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1231 2722 1231
Size: 1491 2864
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 529 656 792 1276 361
Size: 168 127 136 484 3440
Fragments arranged by size:
3440 484 168 136 127
Bpu10I CC'TnA_GC
Cuts at: 883 1216 883
Size: 333 4022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1531 2410 2902 3200 1531
Size: 879 492 298 2686
Fragments arranged by size:
2686 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4059 4059
Size: 4355
BsaAI yAC'GTr
Cuts at: 857 1708 857
Size: 851 3504
BsaBI GATnn'nnATC
Cuts at: 6 1869 3906 6
Size: 1863 2037 455
Fragments arranged by size:
2037 1863 455
BsaHI Gr'CG_yC
Cuts at: 1786 2034 2736 4232 1786
Size: 248 702 1496 1909
Fragments arranged by size:
1909 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1544 2065 2743 3017 3164 3575 1544
Size: 521 678 274 147 411 2324
Fragments arranged by size:
2324 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 379 409 835 865 379
Size: 30 426 30 3869
Fragments arranged by size:
3869 426 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 840 1306 400
Size: 440 466 3449
Fragments arranged by size:
3449 466 440
BseYI C'CCAG_C
Cuts at: 1780 2538 3115 3611 1780
Size: 758 577 496 2524
Fragments arranged by size:
2524 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1314 247
Size: 1067 3288
BsiEI CG_ry'CG
Cuts at: 104 1897 1943 3151 104
Size: 1793 46 1208 1308
Fragments arranged by size:
1793 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 347 1369 2147 2337 3129 347
Size: 1022 778 190 792 1573
Fragments arranged by size:
1573 1022 792 778 190
BsiWI C'GTAC_G
Cuts at: 779 779
Size: 4355
Bsp1286I G_dGCh'C
Cuts at: 347 950 1013 1369 1980 2073 2147 2337 2399
Size: 603 63 356 611 93 74 190 62
Cuts at: 2399 3129 347
Size: 730 1573
Fragments arranged by size:
1573 730 611 603 356 190 93 74
63 62
BspEI T'CCGG_A
Cuts at: 3575 3575
Size: 4355
BspHI T'CATG_A
Cuts at: 930 3531 930
Size: 2601 1754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 419 437 1166 1336 1405 1619 1921 2302 419
Size: 18 729 170 69 214 302 381 2472
Fragments arranged by size:
2472 729 381 302 214 170 69 18
BsrBI CCG'CTC
Cuts at: 50 2647 2701 4185 4350 50
Size: 2597 54 1484 165 55
Fragments arranged by size:
2597 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 263 2267 263
Size: 2004 2351
BsrFI r'CCGG_y
Cuts at: 2353 2534 2743 2353
Size: 181 209 3965
Fragments arranged by size:
3965 209 181
BsrGI T'GTAC_A
Cuts at: 689 689
Size: 4355
BssSI C'ACGA_G
Cuts at: 2626 2984 2626
Size: 358 3997
BstAPI GCAn_nnn'nTGC
Cuts at: 330 453 1732 330
Size: 123 1279 2953
Fragments arranged by size:
2953 1279 123
BstBI TT'CG_AA
Cuts at: 2716 2716
Size: 4355
BstYI r'GATC_y
Cuts at: 1 903 1114 1380 1847 1870 2205 2451 3463
Size: 902 211 266 467 23 335 246 1012
Cuts at: 3463 3549 3561 3907 1
Size: 86 12 346 449
Fragments arranged by size:
1012 902 467 449 346 335 266 246
211 86 23 12
BtgI C'CryG_G
Cuts at: 107 644 1335 107
Size: 537 691 3127
Fragments arranged by size:
3127 691 537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2305 2486 2649 2305
Size: 181 163 4011
Fragments arranged by size:
4011 181 163
BtsI GCAGTG_nn'
Cuts at: 1598 1598
Size: 4355
ClaI AT'CG_AT
Cuts at: 806 806
Size: 4355
DraI TTT'AAA
Cuts at: 1354 1354
Size: 4355
DraIII CAC_nnn'GTG
Cuts at: 860 860
Size: 4355
DrdI GACnn_nn'nnGTC
Cuts at: 2061 2919 2061
Size: 858 3497
EaeI y'GGCC_r
Cuts at: 956 1940 2114 2505 2532 3877 956
Size: 984 174 391 27 1345 1434
Fragments arranged by size:
1434 1345 984 391 174 27
EagI C'GGCC_G
Cuts at: 1940 1940
Size: 4355
EarI CTCTTCn'nnn_
Cuts at: 1049 2378 2588 1049
Size: 1329 210 2816
Fragments arranged by size:
2816 1329 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2563 2873 3019 4165 2563
Size: 310 146 1146 2753
Fragments arranged by size:
2753 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1367 1367
Size: 4355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 502 529 656 792 1276 2180 2612 3359
Size: 141 27 127 136 484 904 432 747
Cuts at: 3359 3627 361
Size: 268 1089
Fragments arranged by size:
1089 904 747 484 432 268 141 136
127 27
EcoNI CCTnn'n_nnAGG
Cuts at: 898 898
Size: 4355
EcoO109I rG'GnC_Cy
Cuts at: 703 1494 703
Size: 791 3564
EcoRI G'AATT_C
Cuts at: 1189 1359 1189
Size: 170 4185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4355
FspI TGC'GCA
Cuts at: 2136 2136
Size: 4355
HaeII r_GCGC'y
Cuts at: 1789 2037 3059 4038 1789
Size: 248 1022 979 2106
Fragments arranged by size:
2106 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 718 750 888 920 2197 2227 2229 2259 718
Size: 32 138 32 1277 30 2 30 2814
Fragments arranged by size:
2814 1277 138 32 32 30 30 2
HincII GTy'rAC
Cuts at: 162 726 1394 162
Size: 564 668 3123
Fragments arranged by size:
3123 668 564
KasI G'GCGC_C
Cuts at: 1785 2033 1785
Size: 248 4107
KpnI G_GTAC'C
Cuts at: 528 1375 528
Size: 847 3508
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3026 3210 3026
Size: 184 4171
MscI TGG'CCA
Cuts at: 2116 2116
Size: 4355
MslI CAynn'nnrTG
Cuts at: 397 403 581 2344 2471 397
Size: 6 178 1763 127 2281
Fragments arranged by size:
2281 1763 178 127 6
MspA1I CmG'CkG
Cuts at: 1139 1146 1466 1780 2140 3153 3398 1139
Size: 7 320 314 360 1013 245 2096
Fragments arranged by size:
2096 1013 360 320 314 245 7
NaeI GCC'GGC
Cuts at: 2536 2536
Size: 4355
NarI GG'CG_CC
Cuts at: 1786 2034 1786
Size: 248 4107
NcoI C'CATG_G
Cuts at: 107 644 107
Size: 537 3818
NgoMIV G'CCGG_C
Cuts at: 2534 2534
Size: 4355
NsiI A_TGCA'T
Cuts at: 223 223
Size: 4355
NspI r_CATG'y
Cuts at: 396 586 825 1408 1908 2815 396
Size: 190 239 583 500 907 1936
Fragments arranged by size:
1936 907 583 500 239 190
PasI CC'CwG_GG
Cuts at: 738 738
Size: 4355
PciI A'CATG_T
Cuts at: 392 582 821 2811 392
Size: 190 239 1990 1936
Fragments arranged by size:
1990 1936 239 190
PflMI CCAn_nnn'nTGG
Cuts at: 1248 1248
Size: 4355
PfoI T'CCnGG_A
Cuts at: 314 377 1181 1292 314
Size: 63 804 111 3377
Fragments arranged by size:
3377 804 111 63
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PmlI CAC'GTG
Cuts at: 857 857
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 379 411 3494 3526 379
Size: 32 3083 32 1208
Fragments arranged by size:
3083 1208 32 32
PstI C_TGCA'G
Cuts at: 326 446 1163 1345 1402 2087 326
Size: 120 717 182 57 685 2594
Fragments arranged by size:
2594 717 685 182 120 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 1139 1780 2140 1139
Size: 641 360 3354
Fragments arranged by size:
3354 641 360
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 4355
SacI G_AGCT'C
Cuts at: 1369 1369
Size: 4355
SalI G'TCGA_C
Cuts at: 724 1392 724
Size: 668 3687
SapI GCTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4355
ScaI AGT'ACT
Cuts at: 3872 3872
Size: 4355
SfcI C'TryA_G
Cuts at: 33 322 442 1159 1341 1398 2083 3076 3267
Size: 289 120 717 182 57 685 993 191
Cuts at: 3267 33
Size: 1121
Fragments arranged by size:
1121 993 717 685 289 191 182 120
57
SfoI GGC'GCC
Cuts at: 1787 2035 1787
Size: 248 4107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SmaI CCC'GGG
Cuts at: 1377 1377
Size: 4355
SmlI C'TyrA_G
Cuts at: 1510 2425 2917 3179 1510
Size: 915 492 262 2686
Fragments arranged by size:
2686 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 1408 1908 1408
Size: 500 3855
StyI C'CwwG_G
Cuts at: 107 644 899 1489 1843 107
Size: 537 255 590 354 2619
Fragments arranged by size:
2619 590 537 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 783 1336 1938 2071 2530 2739 783
Size: 553 602 133 459 209 2399
Fragments arranged by size:
2399 602 553 459 209 133
TatI w'GTAC_w
Cuts at: 263 689 3870 263
Size: 426 3181 748
Fragments arranged by size:
3181 748 426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 835 867 3872 3904 835
Size: 32 3005 32 1286
Fragments arranged by size:
3005 1286 32 32
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4355
XbaI T'CTAG_A
Cuts at: 1065 1386 1065
Size: 321 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 663 1850 663
Size: 1187 3168
XmaI C'CCGG_G
Cuts at: 1375 1375
Size: 4355
XmnI GAAnn'nnTTC
Cuts at: 310 310
Size: 4355
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI ApaI AscI AvrII
BaeI BbsI BcgI BglI BmgBI BmtI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI