Restriction map for
pF1KE0716
(Circular) (Six-base) MAPSORT of: pF1KE0716.seq Check: 4638 from: 1 to: 4349
pF1KE0716 4349 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 611 611
Size: 4349
AccI GT'mk_AC
Cuts at: 1387 1387
Size: 4349
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 1032 2174 2606 3353 3621 130
Size: 902 1142 432 747 268 858
Fragments arranged by size:
1142 902 858 747 432 268
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 963 2737 963
Size: 1774 2575
AleI CACnn'nnGTG
Cuts at: 964 964
Size: 4349
AlwNI CAG_nnn'CTG
Cuts at: 299 1080 1306 1654 3221 299
Size: 781 226 348 1567 1427
Fragments arranged by size:
1567 1427 781 348 226
ApaI G_GGCC'C
Cuts at: 247 662 247
Size: 415 3934
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 556 1353 3702 3958 556
Size: 797 2349 256 947
Fragments arranged by size:
2349 947 797 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 341 451 471 661 983 1150 1196 1369 341
Size: 110 20 190 322 167 46 173 3321
Fragments arranged by size:
3321 322 190 173 167 110 46 20
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 176 187 596 966 1365 1779 2027 2062 176
Size: 11 409 370 399 414 248 35 2463
Fragments arranged by size:
2463 414 409 399 370 248 35 11
BanII G_rGCy'C
Cuts at: 247 470 662 1151 1196 1363 2393 247
Size: 223 192 489 45 167 1030 2203
Fragments arranged by size:
2203 1030 489 223 192 167 45
BbeI G_GCGC'C
Cuts at: 180 191 1783 2031 180
Size: 11 1592 248 2498
Fragments arranged by size:
2498 1592 248 11
BbvCI CC'TCA_GC
Cuts at: 1073 1073
Size: 4349
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglI GCCn_nnn'nGGC
Cuts at: 185 1269 185
Size: 1084 3265
BglII A'GATC_T
Cuts at: 806 1864 3901 806
Size: 1058 2037 1254
Fragments arranged by size:
2037 1254 1058
BlpI GC'TnA_GC
Cuts at: 1461 1461
Size: 4349
Bme1580I G_kGCm'C
Cuts at: 247 642 662 1013 1974 2067 3123 247
Size: 395 20 351 961 93 1056 1473
Fragments arranged by size:
1473 1056 961 395 351 93 20
BmrI ACTGGGnnnn_n'
Cuts at: 429 1561 1642 1977 4121 429
Size: 1132 81 335 2144 657
Fragments arranged by size:
2144 1132 657 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 849 2716 849
Size: 1867 2482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 385 664 785 1234 250
Size: 135 279 121 449 3365
Fragments arranged by size:
3365 449 279 135 121
Bpu10I CC'TnA_GC
Cuts at: 1073 1073
Size: 4349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1525 2404 2896 3194 1525
Size: 879 492 298 2680
Fragments arranged by size:
2680 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 361 567 1105 4053 361
Size: 206 538 2948 657
Fragments arranged by size:
2948 657 538 206
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 177 188 1780 2028 2730 4226 177
Size: 11 1592 248 702 1496 300
Fragments arranged by size:
1592 1496 702 300 248 11
BsaWI w'CCGG_w
Cuts at: 963 1538 2059 2737 3011 3158 3569 963
Size: 575 521 678 274 147 411 1743
Fragments arranged by size:
1743 678 575 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 367 397 985 1015 367
Size: 30 588 30 3701
Fragments arranged by size:
3701 588 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1061 1123 1227 1061
Size: 62 104 4183
Fragments arranged by size:
4183 104 62
BseYI C'CCAG_C
Cuts at: 628 674 1774 2532 3109 3605 628
Size: 46 1100 758 577 496 1372
Fragments arranged by size:
1372 1100 758 577 496 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 836 445
Size: 391 3958
BsiEI CG_ry'CG
Cuts at: 104 928 1891 1937 3145 104
Size: 824 963 46 1208 1308
Fragments arranged by size:
1308 1208 963 824 46
BsiHKAI G_wGCw'C
Cuts at: 470 1363 2141 2331 3123 470
Size: 893 778 190 792 1696
Fragments arranged by size:
1696 893 792 778 190
BsmI GAATG_Cn'
Cuts at: 852 852
Size: 4349
BsmBI CGTCTCn'nnnn_
Cuts at: 107 765 107
Size: 658 3691
Bsp1286I G_dGCh'C
Cuts at: 247 470 642 662 1013 1151 1196 1363 1974
Size: 223 172 20 351 138 45 167 611
Cuts at: 1974 2067 2141 2331 2393 3123 247
Size: 93 74 190 62 730 1473
Fragments arranged by size:
1473 730 611 351 223 190 172 167
138 93 74 62 45 20
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 611 1399 1613 1915 2296 611
Size: 788 214 302 381 2664
Fragments arranged by size:
2664 788 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2641 2695 4179 4344 50
Size: 2591 54 1484 165 55
Fragments arranged by size:
2591 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 807 2261 807
Size: 1454 2895
BsrFI r'CCGG_y
Cuts at: 319 494 963 2347 2528 2737 319
Size: 175 469 1384 181 209 1931
Fragments arranged by size:
1931 1384 469 209 181 175
BsrGI T'GTAC_A
Cuts at: 521 521
Size: 4349
BssSI C'ACGA_G
Cuts at: 2620 2978 2620
Size: 358 3991
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 785 2710 785
Size: 1925 2424
BstEII G'GTnAC_C
Cuts at: 726 726
Size: 4349
BstYI r'GATC_y
Cuts at: 1 806 1374 1841 1864 2199 2445 3457 3543
Size: 805 568 467 23 335 246 1012 86
Cuts at: 3543 3555 3901 1
Size: 12 346 449
Fragments arranged by size:
1012 805 568 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 976 107
Size: 869 3480
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2299 2480 2643 2299
Size: 181 163 4005
Fragments arranged by size:
4005 181 163
BtsI GCAGTG_nn'
Cuts at: 128 937 1592 128
Size: 809 655 2885
Fragments arranged by size:
2885 809 655
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 311 925 1082 1934 2108 2499 2526 3871 311
Size: 614 157 852 174 391 27 1345 789
Fragments arranged by size:
1345 852 789 614 391 174 157 27
EagI C'GGCC_G
Cuts at: 925 1934 925
Size: 1009 3340
EarI CTCTTCn'nnn_
Cuts at: 818 2372 2582 818
Size: 1554 210 2585
Fragments arranged by size:
2585 1554 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 277 338 2557 2867 3013 4159 277
Size: 61 2219 310 146 1146 467
Fragments arranged by size:
2219 1146 467 310 146 61
EcoICRI GAG'CTC
Cuts at: 468 1361 468
Size: 893 3456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 250 385 664 785 1032 1234 2174 2606
Size: 120 135 279 121 247 202 940 432
Cuts at: 2606 3353 3621 130
Size: 747 268 858
Fragments arranged by size:
940 858 747 432 279 268 247 202
135 121 120
EcoNI CCTnn'n_nnAGG
Cuts at: 823 823
Size: 4349
EcoO109I rG'GnC_Cy
Cuts at: 243 611 671 1236 1488 243
Size: 368 60 565 252 3104
Fragments arranged by size:
3104 565 368 252 60
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 747 2368 747
Size: 1621 2728
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 180 191 381 711 1783 2031 3053 4032 180
Size: 11 190 330 1072 248 1022 979 497
Fragments arranged by size:
1072 1022 979 497 330 248 190 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2191 2221 2223 2253 2191
Size: 30 2 30 4287
Fragments arranged by size:
4287 30 30 2
HincII GTy'rAC
Cuts at: 1388 1388
Size: 4349
KasI G'GCGC_C
Cuts at: 176 187 1779 2027 176
Size: 11 1592 248 2498
Fragments arranged by size:
2498 1592 248 11
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 3020 3204 188
Size: 2832 184 1333
Fragments arranged by size:
2832 1333 184
MscI TGG'CCA
Cuts at: 2110 2110
Size: 4349
MslI CAynn'nnrTG
Cuts at: 964 2338 2465 964
Size: 1374 127 2848
Fragments arranged by size:
2848 1374 127
MspA1I CmG'CkG
Cuts at: 406 678 1228 1460 1774 2134 3147 3392 406
Size: 272 550 232 314 360 1013 245 1363
Fragments arranged by size:
1363 1013 550 360 314 272 245 232
NaeI GCC'GGC
Cuts at: 321 496 2530 321
Size: 175 2034 2140
Fragments arranged by size:
2140 2034 175
NarI GG'CG_CC
Cuts at: 177 188 1780 2028 177
Size: 11 1592 248 2498
Fragments arranged by size:
2498 1592 248 11
NcoI C'CATG_G
Cuts at: 107 107
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 319 494 2528 319
Size: 175 2034 2140
Fragments arranged by size:
2140 2034 175
NspI r_CATG'y
Cuts at: 1258 1402 1902 2809 1258
Size: 144 500 907 2798
Fragments arranged by size:
2798 907 500 144
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PfoI T'CCnGG_A
Cuts at: 362 362
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PpuMI rG'GwC_Cy
Cuts at: 611 671 1236 611
Size: 60 565 3724
Fragments arranged by size:
3724 565 60
PspOMI G'GGCC_C
Cuts at: 243 658 243
Size: 415 3934
PstI C_TGCA'G
Cuts at: 239 405 1396 2081 239
Size: 166 991 685 2507
Fragments arranged by size:
2507 991 685 166
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 678 1228 1774 2134 678
Size: 550 546 360 2893
Fragments arranged by size:
2893 550 546 360
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 470 1363 470
Size: 893 3456
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SanDI GG'GwC_CC
Cuts at: 671 1236 671
Size: 565 3784
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SfcI C'TryA_G
Cuts at: 33 235 401 578 693 1392 2077 3070 3261
Size: 202 166 177 115 699 685 993 191
Cuts at: 3261 33
Size: 1121
Fragments arranged by size:
1121 993 699 685 202 191 177 166
115
SfoI GGC'GCC
Cuts at: 178 189 1781 2029 178
Size: 11 1592 248 2498
Fragments arranged by size:
2498 1592 248 11
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SgrAI Cr'CCGG_yG
Cuts at: 963 963
Size: 4349
SmaI CCC'GGG
Cuts at: 343 453 1198 1371 343
Size: 110 745 173 3321
Fragments arranged by size:
3321 745 173 110
SmlI C'TyrA_G
Cuts at: 1504 2419 2911 3173 1504
Size: 915 492 262 2680
Fragments arranged by size:
2680 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StyI C'CwwG_G
Cuts at: 107 218 371 1034 1483 1837 107
Size: 111 153 663 449 354 2619
Fragments arranged by size:
2619 663 449 354 153 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 411 969 1932 2065 2524 2733 411
Size: 558 963 133 459 209 2027
Fragments arranged by size:
2027 963 558 459 209 133
TatI w'GTAC_w
Cuts at: 521 1323 3864 521
Size: 802 2541 1006
Fragments arranged by size:
2541 1006 802
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4317
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 438 1844 438
Size: 1406 2943
XmaI C'CCGG_G
Cuts at: 341 451 1196 1369 341
Size: 110 745 173 3321
Fragments arranged by size:
3321 745 173 110
XmnI GAAnn'nnTTC
Cuts at: 1045 1045
Size: 4349
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI ZraI