Restriction map for
pF1KE0715
(Circular) (Six-base) MAPSORT of: pF1KE0715.seq Check: 1945 from: 1 to: 4025
pF1KE0715 4025 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 686 686
Size: 4025
AccI GT'mk_AC
Cuts at: 1063 1063
Size: 4025
Acc65I G'GTAC_C
Cuts at: 1041 1041
Size: 4025
AclI AA'CG_TT
Cuts at: 3865 3865
Size: 4025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 853 1850 2282 3029 3297 623
Size: 230 997 432 747 268 1351
Fragments arranged by size:
1351 997 747 432 268 230
AflIII A'CryG_T
Cuts at: 1377 2481 3458 1377
Size: 1104 977 1944
Fragments arranged by size:
1944 1104 977
AgeI A'CCGG_T
Cuts at: 2413 2413
Size: 4025
AleI CACnn'nnGTG
Cuts at: 757 757
Size: 4025
AlwNI CAG_nnn'CTG
Cuts at: 754 1330 2897 754
Size: 576 1567 1882
Fragments arranged by size:
1882 1567 576
ApaLI G'TGCA_C
Cuts at: 2795 2795
Size: 4025
ApoI r'AATT_y
Cuts at: 1029 3378 3634 1029
Size: 2349 256 1420
Fragments arranged by size:
2349 1420 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4025
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 413 1045 413
Size: 632 3393
BamHI G'GATC_C
Cuts at: 1050 1050
Size: 4025
BanI G'GyrC_C
Cuts at: 1041 1455 1703 1738 1041
Size: 414 248 35 3328
Fragments arranged by size:
3328 414 248 35
BanII G_rGCy'C
Cuts at: 128 904 1039 2069 128
Size: 776 135 1030 2084
Fragments arranged by size:
2084 1030 776 135
BbeI G_GCGC'C
Cuts at: 1459 1707 1459
Size: 248 3777
BbsI GAAGACnn'nnnn_
Cuts at: 1004 1004
Size: 4025
BciVI GTATCCnnnnn_n'
Cuts at: 1923 2684 4025 1923
Size: 761 1341 1923
Fragments arranged by size:
1923 1341 761
BclI T'GATC_A
Cuts at: 1545 1545
Size: 4025
BglII A'GATC_T
Cuts at: 1540 3577 1540
Size: 2037 1988
BlpI GC'TnA_GC
Cuts at: 1137 1137
Size: 4025
Bme1580I G_kGCm'C
Cuts at: 1650 1743 2799 1650
Size: 93 1056 2876
Fragments arranged by size:
2876 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1237 1318 1653 3797 1237
Size: 81 335 2144 1465
Fragments arranged by size:
2144 1465 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 4025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 544 427
Size: 117 3908
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1201 2080 2572 2870 1201
Size: 879 492 298 2356
Fragments arranged by size:
2356 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 4025
BsaAI yAC'GTr
Cuts at: 1378 1378
Size: 4025
BsaBI GATnn'nnATC
Cuts at: 6 1539 3576 6
Size: 1533 2037 455
Fragments arranged by size:
2037 1533 455
BsaHI Gr'CG_yC
Cuts at: 683 1456 1704 2406 3902 683
Size: 773 248 702 1496 806
Fragments arranged by size:
1496 806 773 702 248
BsaWI w'CCGG_w
Cuts at: 1214 1735 2413 2687 2834 3245 1214
Size: 521 678 274 147 411 1994
Fragments arranged by size:
1994 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 643 673 643
Size: 30 3995
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 460 469 178
Size: 282 9 3734
Fragments arranged by size:
3734 282 9
BseYI C'CCAG_C
Cuts at: 1450 2208 2785 3281 1450
Size: 758 577 496 2194
Fragments arranged by size:
2194 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 962 1567 1613 2821 104
Size: 858 605 46 1208 1308
Fragments arranged by size:
1308 1208 858 605 46
BsiHKAI G_wGCw'C
Cuts at: 128 469 1039 1817 2007 2799 128
Size: 341 570 778 190 792 1354
Fragments arranged by size:
1354 792 778 570 341 190
Bsp1286I G_dGCh'C
Cuts at: 128 469 904 1039 1650 1743 1817 2007 2069
Size: 341 435 135 611 93 74 190 62
Cuts at: 2069 2799 128
Size: 730 1354
Fragments arranged by size:
1354 730 611 435 341 190 135 93
74 62
BspEI T'CCGG_A
Cuts at: 3245 3245
Size: 4025
BspHI T'CATG_A
Cuts at: 938 3201 938
Size: 2263 1762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1075 1289 1591 1972 1075
Size: 214 302 381 3128
Fragments arranged by size:
3128 381 302 214
BsrBI CCG'CTC
Cuts at: 50 420 966 2317 2371 3855 4020 50
Size: 370 546 1351 54 1484 165 55
Fragments arranged by size:
1484 1351 546 370 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1937 1937
Size: 4025
BsrFI r'CCGG_y
Cuts at: 2023 2204 2413 2023
Size: 181 209 3635
Fragments arranged by size:
3635 209 181
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 1402 1402
Size: 4025
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstYI r'GATC_y
Cuts at: 1 1050 1517 1540 1875 2121 3133 3219 3231
Size: 1049 467 23 335 246 1012 86 12
Cuts at: 3231 3577 1
Size: 346 449
Fragments arranged by size:
1049 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 869 107
Size: 762 3263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1975 2156 2319 1975
Size: 181 163 3681
Fragments arranged by size:
3681 181 163
BtsI GCAGTG_nn'
Cuts at: 119 752 1268 119
Size: 633 516 2876
Fragments arranged by size:
2876 633 516
DraI TTT'AAA
Cuts at: 1024 1024
Size: 4025
DrdI GACnn_nn'nnGTC
Cuts at: 1731 2589 1731
Size: 858 3167
EaeI y'GGCC_r
Cuts at: 959 1610 1784 2175 2202 3547 959
Size: 651 174 391 27 1345 1437
Fragments arranged by size:
1437 1345 651 391 174 27
EagI C'GGCC_G
Cuts at: 959 1610 959
Size: 651 3374
EarI CTCTTCn'nnn_
Cuts at: 268 446 500 2048 2258 268
Size: 178 54 1548 210 2035
Fragments arranged by size:
2035 1548 210 178 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2233 2543 2689 3835 2233
Size: 310 146 1146 2423
Fragments arranged by size:
2423 1146 310 146
EcoICRI GAG'CTC
Cuts at: 126 1037 126
Size: 911 3114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 544 623 853 1850 2282 3029 3297 427
Size: 117 79 230 997 432 747 268 1155
Fragments arranged by size:
1155 997 747 432 268 230 117 79
EcoO109I rG'GnC_Cy
Cuts at: 210 362 1164 210
Size: 152 802 3071
Fragments arranged by size:
3071 802 152
EcoRI G'AATT_C
Cuts at: 1029 1029
Size: 4025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 144 415 2044 144
Size: 271 1629 2125
Fragments arranged by size:
2125 1629 271
FspI TGC'GCA
Cuts at: 946 1806 946
Size: 860 3165
FspAI rTGC'GCAy
Cuts at: 946 946
Size: 4025
HaeII r_GCGC'y
Cuts at: 1459 1707 2729 3708 1459
Size: 248 1022 979 1776
Fragments arranged by size:
1776 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 643 675 1867 1897 1899 1929 643
Size: 32 1192 30 2 30 2739
Fragments arranged by size:
2739 1192 32 30 30 2
HincII GTy'rAC
Cuts at: 1064 1064
Size: 4025
HindIII A'AGCT_T
Cuts at: 397 397
Size: 4025
KasI G'GCGC_C
Cuts at: 1455 1703 1455
Size: 248 3777
KpnI G_GTAC'C
Cuts at: 1045 1045
Size: 4025
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 827 2696 2880 827
Size: 1869 184 1972
Fragments arranged by size:
1972 1869 184
MscI TGG'CCA
Cuts at: 1786 1786
Size: 4025
MslI CAynn'nnrTG
Cuts at: 112 757 2014 2141 112
Size: 645 1257 127 1996
Fragments arranged by size:
1996 1257 645 127
MspA1I CmG'CkG
Cuts at: 175 256 522 787 975 1136 1450 1810 2823
Size: 81 266 265 188 161 314 360 1013
Cuts at: 2823 3068 175
Size: 245 1132
Fragments arranged by size:
1132 1013 360 314 266 265 245 188
161 81
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 4025
NarI GG'CG_CC
Cuts at: 1456 1704 1456
Size: 248 3777
NcoI C'CATG_G
Cuts at: 107 107
Size: 4025
NdeI CA'TA_TG
Cuts at: 321 321
Size: 4025
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 4025
NspI r_CATG'y
Cuts at: 951 1078 1578 2485 951
Size: 127 500 907 2491
Fragments arranged by size:
2491 907 500 127
PciI A'CATG_T
Cuts at: 2481 2481
Size: 4025
PfoI T'CCnGG_A
Cuts at: 545 545
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3993
PpuMI rG'GwC_Cy
Cuts at: 362 362
Size: 4025
PstI C_TGCA'G
Cuts at: 309 1072 1757 309
Size: 763 685 2577
Fragments arranged by size:
2577 763 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 256 522 975 1450 1810 256
Size: 266 453 475 360 2471
Fragments arranged by size:
2471 475 453 360 266
RsrII CG'GwC_CG
Cuts at: 2220 2220
Size: 4025
SacI G_AGCT'C
Cuts at: 128 1039 128
Size: 911 3114
SalI G'TCGA_C
Cuts at: 1062 1062
Size: 4025
SapI GCTCTTCn'nnn_
Cuts at: 2048 2258 2048
Size: 210 3815
SbfI CC_TGCA'GG
Cuts at: 1072 1072
Size: 4025
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SexAI A'CCwGG_T
Cuts at: 725 725
Size: 4025
SfcI C'TryA_G
Cuts at: 33 305 1068 1753 2746 2937 33
Size: 272 763 685 993 191 1121
Fragments arranged by size:
1121 993 763 685 272 191
SfoI GGC'GCC
Cuts at: 1457 1705 1457
Size: 248 3777
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SmaI CCC'GGG
Cuts at: 415 1047 415
Size: 632 3393
SmlI C'TyrA_G
Cuts at: 1180 2095 2587 2849 1180
Size: 915 492 262 2356
Fragments arranged by size:
2356 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 951 1078 1578 951
Size: 127 500 3398
Fragments arranged by size:
3398 500 127
StuI AGG'CCT
Cuts at: 283 283
Size: 4025
StyI C'CwwG_G
Cuts at: 107 119 284 609 1159 1513 107
Size: 12 165 325 550 354 2619
Fragments arranged by size:
2619 550 354 325 165 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 239 313 1608 1741 2200 2409 239
Size: 74 1295 133 459 209 1855
Fragments arranged by size:
1855 1295 459 209 133 74
TatI w'GTAC_w
Cuts at: 3540 3540
Size: 4025
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 3993
Tth111I GACn'n_nGTC
Cuts at: 328 1822 328
Size: 1494 2531
XbaI T'CTAG_A
Cuts at: 1056 1056
Size: 4025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 808 1520 808
Size: 712 3313
XmaI C'CCGG_G
Cuts at: 413 1045 413
Size: 632 3393
ZraI GAC'GTC
Cuts at: 684 684
Size: 4025
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI