Restriction map for
pF1KE0712
(Circular) (Six-base) MAPSORT of: pF1KE0712.seq Check: 1398 from: 1 to: 4346
pF1KE0712 4346 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 604 604
Size: 4346
AccI GT'mk_AC
Cuts at: 719 1322 1384 719
Size: 603 62 3681
Fragments arranged by size:
3681 603 62
Acc65I G'GTAC_C
Cuts at: 1362 1362
Size: 4346
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 2171 2603 3350 3618 609
Size: 1562 432 747 268 1337
Fragments arranged by size:
1562 1337 747 432 268
AfeI AGC'GCT
Cuts at: 214 419 214
Size: 205 4141
AflIII A'CryG_T
Cuts at: 453 1698 2802 3779 453
Size: 1245 1104 977 1020
Fragments arranged by size:
1245 1104 1020 977
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AleI CACnn'nnGTG
Cuts at: 301 301
Size: 4346
AlwNI CAG_nnn'CTG
Cuts at: 1651 3218 1651
Size: 1567 2779
ApaI G_GGCC'C
Cuts at: 986 986
Size: 4346
ApaLI G'TGCA_C
Cuts at: 3116 3116
Size: 4346
ApoI r'AATT_y
Cuts at: 1350 3699 3955 1350
Size: 2349 256 1741
Fragments arranged by size:
2349 1741 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 273 1366 273
Size: 1093 3253
BamHI G'GATC_C
Cuts at: 1371 1371
Size: 4346
BanI G'GyrC_C
Cuts at: 739 1314 1362 1776 2024 2059 739
Size: 575 48 414 248 35 3026
Fragments arranged by size:
3026 575 414 248 48 35
BanII G_rGCy'C
Cuts at: 280 287 986 1360 2390 280
Size: 7 699 374 1030 2236
Fragments arranged by size:
2236 1030 699 374 7
BbeI G_GCGC'C
Cuts at: 1780 2028 1780
Size: 248 4098
BbsI GAAGACnn'nnnn_
Cuts at: 450 450
Size: 4346
BciVI GTATCCnnnnn_n'
Cuts at: 2244 3005 4346 2244
Size: 761 1341 2244
Fragments arranged by size:
2244 1341 761
BclI T'GATC_A
Cuts at: 1866 1866
Size: 4346
BglII A'GATC_T
Cuts at: 572 1238 1861 3898 572
Size: 666 623 2037 1020
Fragments arranged by size:
2037 1020 666 623
BlpI GC'TnA_GC
Cuts at: 1458 1458
Size: 4346
Bme1580I G_kGCm'C
Cuts at: 295 986 1971 2064 3120 295
Size: 691 985 93 1056 1521
Fragments arranged by size:
1521 1056 985 691 93
BmrI ACTGGGnnnn_n'
Cuts at: 391 701 1558 1639 1974 4118 391
Size: 310 857 81 335 2144 619
Fragments arranged by size:
2144 857 619 335 310 81
BmtI G_CTAG'C
Cuts at: 473 473
Size: 4346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 591 2713 591
Size: 2122 2224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 184 750 869 1073 169
Size: 15 566 119 204 3442
Fragments arranged by size:
3442 566 204 119 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 271 1210 1522 2401 2893 3191 211
Size: 60 939 312 879 492 298 1366
Fragments arranged by size:
1366 939 879 492 312 298 60
BsaI GGTCTCn'nnnn_
Cuts at: 718 4050 718
Size: 3332 1014
BsaAI yAC'GTr
Cuts at: 1699 1699
Size: 4346
BsaBI GATnn'nnATC
Cuts at: 6 1860 3897 6
Size: 1854 2037 455
Fragments arranged by size:
2037 1854 455
BsaHI Gr'CG_yC
Cuts at: 313 601 1325 1777 2025 2727 4223 313
Size: 288 724 452 248 702 1496 436
Fragments arranged by size:
1496 724 702 452 436 288 248
BsaWI w'CCGG_w
Cuts at: 138 1535 2056 2734 3008 3155 3566 138
Size: 1397 521 678 274 147 411 918
Fragments arranged by size:
1397 918 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 792 822 935 965 792
Size: 30 113 30 4173
Fragments arranged by size:
4173 113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 874 943 583
Size: 291 69 3986
Fragments arranged by size:
3986 291 69
BseYI C'CCAG_C
Cuts at: 900 1131 1771 2529 3106 3602 900
Size: 231 640 758 577 496 1644
Fragments arranged by size:
1644 758 640 577 496 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 421
Size: 4346
BsiEI CG_ry'CG
Cuts at: 104 1116 1888 1934 3142 104
Size: 1012 772 46 1208 1308
Fragments arranged by size:
1308 1208 1012 772 46
BsiHKAI G_wGCw'C
Cuts at: 287 565 1360 2138 2328 3120 287
Size: 278 795 778 190 792 1513
Fragments arranged by size:
1513 795 792 778 278 190
BsmBI CGTCTCn'nnnn_
Cuts at: 180 180
Size: 4346
Bsp1286I G_dGCh'C
Cuts at: 280 287 295 565 986 1360 1971 2064 2138
Size: 7 8 270 421 374 611 93 74
Cuts at: 2138 2328 2390 3120 280
Size: 190 62 730 1506
Fragments arranged by size:
1506 730 611 421 374 270 190 93
74 62 8 7
BspEI T'CCGG_A
Cuts at: 3566 3566
Size: 4346
BspHI T'CATG_A
Cuts at: 3522 3522
Size: 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1396 1610 1912 2293 1396
Size: 214 302 381 3449
Fragments arranged by size:
3449 381 302 214
BsrBI CCG'CTC
Cuts at: 50 764 813 2638 2692 4176 4341 50
Size: 714 49 1825 54 1484 165 55
Fragments arranged by size:
1825 1484 714 165 55 54 49
BsrDI GCAATG_nn'
Cuts at: 592 2258 592
Size: 1666 2680
BsrFI r'CCGG_y
Cuts at: 2344 2525 2734 2344
Size: 181 209 3956
Fragments arranged by size:
3956 209 181
BssSI C'ACGA_G
Cuts at: 2617 2975 2617
Size: 358 3988
BstAPI GCAn_nnn'nTGC
Cuts at: 1723 1723
Size: 4346
BstBI TT'CG_AA
Cuts at: 2707 2707
Size: 4346
BstYI r'GATC_y
Cuts at: 1 572 1238 1371 1838 1861 2196 2442 3454
Size: 571 666 133 467 23 335 246 1012
Cuts at: 3454 3540 3552 3898 1
Size: 86 12 346 449
Fragments arranged by size:
1012 666 571 467 449 346 335 246
133 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 540 2296 2477 2640 540
Size: 1756 181 163 2246
Fragments arranged by size:
2246 1756 181 163
BtsI GCAGTG_nn'
Cuts at: 325 1299 1589 325
Size: 974 290 3082
Fragments arranged by size:
3082 974 290
DraI TTT'AAA
Cuts at: 1345 1345
Size: 4346
DrdI GACnn_nn'nnGTC
Cuts at: 2052 2910 2052
Size: 858 3488
EaeI y'GGCC_r
Cuts at: 149 1931 2105 2496 2523 3868 149
Size: 1782 174 391 27 1345 627
Fragments arranged by size:
1782 1345 627 391 174 27
EagI C'GGCC_G
Cuts at: 1931 1931
Size: 4346
EarI CTCTTCn'nnn_
Cuts at: 928 2369 2579 928
Size: 1441 210 2695
Fragments arranged by size:
2695 1441 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 168 604 2554 2864 3010 4156 168
Size: 436 1950 310 146 1146 358
Fragments arranged by size:
1950 1146 436 358 310 146
EcoICRI GAG'CTC
Cuts at: 285 1358 285
Size: 1073 3273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 184 609 750 869 1073 2171 2603 3350
Size: 15 425 141 119 204 1098 432 747
Cuts at: 3350 3618 169
Size: 268 897
Fragments arranged by size:
1098 897 747 432 425 268 204 141
119 15
EcoNI CCTnn'n_nnAGG
Cuts at: 360 970 360
Size: 610 3736
EcoO109I rG'GnC_Cy
Cuts at: 366 865 982 1308 1485 366
Size: 499 117 326 177 3227
Fragments arranged by size:
3227 499 326 177 117
EcoRI G'AATT_C
Cuts at: 1350 1350
Size: 4346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 4346
FspI TGC'GCA
Cuts at: 2127 2127
Size: 4346
HaeII r_GCGC'y
Cuts at: 216 421 1179 1780 2028 3050 4029 216
Size: 205 758 601 248 1022 979 533
Fragments arranged by size:
1022 979 758 601 533 248 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 865 897 935 967 1046 1078 2188 2218 2220
Size: 32 38 32 79 32 1110 30 2
Cuts at: 2220 2250 865
Size: 30 2961
Fragments arranged by size:
2961 1110 79 38 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1323 1385 1323
Size: 62 4284
KasI G'GCGC_C
Cuts at: 1776 2024 1776
Size: 248 4098
KpnI G_GTAC'C
Cuts at: 1366 1366
Size: 4346
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3017 3201 3017
Size: 184 4162
MscI TGG'CCA
Cuts at: 2107 2107
Size: 4346
MslI CAynn'nnrTG
Cuts at: 301 1069 2335 2462 301
Size: 768 1266 127 2185
Fragments arranged by size:
2185 1266 768 127
MspA1I CmG'CkG
Cuts at: 1457 1771 2131 3144 3389 1457
Size: 314 360 1013 245 2414
Fragments arranged by size:
2414 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2527 2527
Size: 4346
NarI GG'CG_CC
Cuts at: 1777 2025 1777
Size: 248 4098
NgoMIV G'CCGG_C
Cuts at: 2525 2525
Size: 4346
NheI G'CTAG_C
Cuts at: 469 469
Size: 4346
NspI r_CATG'y
Cuts at: 457 1399 1899 2806 457
Size: 942 500 907 1997
Fragments arranged by size:
1997 942 907 500
PasI CC'CwG_GG
Cuts at: 166 166
Size: 4346
PciI A'CATG_T
Cuts at: 453 2802 453
Size: 2349 1997
PflMI CCAn_nnn'nTGG
Cuts at: 691 691
Size: 4346
PfoI T'CCnGG_A
Cuts at: 185 446 939 1195 185
Size: 261 493 256 3336
Fragments arranged by size:
3336 493 261 256
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 4314
PspOMI G'GGCC_C
Cuts at: 982 982
Size: 4346
PstI C_TGCA'G
Cuts at: 1049 1393 2078 1049
Size: 344 685 3317
Fragments arranged by size:
3317 685 344
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 1771 2131 1771
Size: 360 3986
RsrII CG'GwC_CG
Cuts at: 2541 2541
Size: 4346
SacI G_AGCT'C
Cuts at: 287 1360 287
Size: 1073 3273
SalI G'TCGA_C
Cuts at: 1321 1383 1321
Size: 62 4284
SapI GCTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
SbfI CC_TGCA'GG
Cuts at: 1393 1393
Size: 4346
ScaI AGT'ACT
Cuts at: 3863 3863
Size: 4346
SfcI C'TryA_G
Cuts at: 33 1045 1389 2074 3067 3258 33
Size: 1012 344 685 993 191 1121
Fragments arranged by size:
1121 1012 993 685 344 191
SfoI GGC'GCC
Cuts at: 1778 2026 1778
Size: 248 4098
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 1368 1368
Size: 4346
SmlI C'TyrA_G
Cuts at: 226 286 1225 1501 2416 2908 3170 226
Size: 60 939 276 915 492 262 1402
Fragments arranged by size:
1402 939 915 492 276 262 60
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 1399 1899 1399
Size: 500 3846
StyI C'CwwG_G
Cuts at: 1480 1834 1480
Size: 354 3992
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 169 363 702 841 1929 2062 2521 2730
Size: 42 194 339 139 1088 133 459 209
Cuts at: 2730 127
Size: 1743
Fragments arranged by size:
1743 1088 459 339 209 194 139 133
42
TatI w'GTAC_w
Cuts at: 989 3861 989
Size: 2872 1474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 792 824 3863 3895 792
Size: 32 3039 32 1243
Fragments arranged by size:
3039 1243 32 32
Tth111I GACn'n_nGTC
Cuts at: 715 2143 715
Size: 1428 2918
XbaI T'CTAG_A
Cuts at: 1377 1377
Size: 4346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1841 1841
Size: 4346
XmaI C'CCGG_G
Cuts at: 1366 1366
Size: 4346
XmnI GAAnn'nnTTC
Cuts at: 262 262
Size: 4346
ZraI GAC'GTC
Cuts at: 602 602
Size: 4346
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI Bpu10I BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI