Restriction map for
pF1KE0711
(Circular) (Six-base) MAPSORT of: pF1KE0711.seq Check: 8812 from: 1 to: 4382
pF1KE0711 4382 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 794 794
Size: 4382
AccI GT'mk_AC
Cuts at: 1016 1420 1016
Size: 404 3978
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 2207 2639 3386 3654 703
Size: 1504 432 747 268 1431
Fragments arranged by size:
1504 1431 747 432 268
AflIII A'CryG_T
Cuts at: 1734 2838 3815 1734
Size: 1104 977 2301
Fragments arranged by size:
2301 1104 977
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AleI CACnn'nnGTG
Cuts at: 122 122
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 873 1687 3254 873
Size: 814 1567 2001
Fragments arranged by size:
2001 1567 814
ApaI G_GGCC'C
Cuts at: 353 1243 353
Size: 890 3492
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 1386 3735 3991 1386
Size: 2349 256 1777
Fragments arranged by size:
2349 1777 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 737 1402 737
Size: 665 3717
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 393 782 863 1398 1812 2060 2095 393
Size: 389 81 535 414 248 35 2680
Fragments arranged by size:
2680 535 414 389 248 81 35
BanII G_rGCy'C
Cuts at: 247 353 938 1243 1261 1396 2426 247
Size: 106 585 305 18 135 1030 2203
Fragments arranged by size:
2203 1030 585 305 135 106 18
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 189 642 189
Size: 453 3929
BbvCI CC'TCA_GC
Cuts at: 820 820
Size: 4382
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 530 1902 530
Size: 1372 3010
BglI GCCn_nnn'nGGC
Cuts at: 391 742 1238 391
Size: 351 496 3535
Fragments arranged by size:
3535 496 351
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 709 1494 709
Size: 785 3597
Bme1580I G_kGCm'C
Cuts at: 353 785 838 1243 2007 2100 3156 353
Size: 432 53 405 764 93 1056 1579
Fragments arranged by size:
1579 1056 764 432 405 93 53
BmrI ACTGGGnnnn_n'
Cuts at: 1237 1594 1675 2010 4154 1237
Size: 357 81 335 2144 1465
Fragments arranged by size:
2144 1465 357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 365 430 904 1257 172
Size: 193 65 474 353 3297
Fragments arranged by size:
3297 474 353 193 65
Bpu10I CC'TnA_GC
Cuts at: 161 241 793 820 161
Size: 80 552 27 3723
Fragments arranged by size:
3723 552 80 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1558 2437 2929 3227 1558
Size: 879 492 298 2713
Fragments arranged by size:
2713 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 568 1350 4086 568
Size: 782 2736 864
Fragments arranged by size:
2736 864 782
BsaAI yAC'GTr
Cuts at: 705 1735 705
Size: 1030 3352
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 1813 2061 2763 4259 1813
Size: 248 702 1496 1936
Fragments arranged by size:
1936 1496 702 248
BsaWI w'CCGG_w
Cuts at: 944 1571 2092 2770 3044 3191 3602 944
Size: 627 521 678 274 147 411 1724
Fragments arranged by size:
1724 678 627 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1243 1255 1273 1285 1243
Size: 12 18 12 4340
Fragments arranged by size:
4340 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 255 258 294 556 763 1356 255
Size: 3 36 262 207 593 3281
Fragments arranged by size:
3281 593 262 207 36 3
BseYI C'CCAG_C
Cuts at: 1036 1215 1807 2565 3142 3638 1036
Size: 179 592 758 577 496 1780
Fragments arranged by size:
1780 758 592 577 496 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1089 1354 712
Size: 377 265 3740
Fragments arranged by size:
3740 377 265
BsiEI CG_ry'CG
Cuts at: 104 1924 1970 3178 104
Size: 1820 46 1208 1308
Fragments arranged by size:
1820 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 167 247 799 1169 1261 1396 2174 2364 3156
Size: 80 552 370 92 135 778 190 792
Cuts at: 3156 167
Size: 1393
Fragments arranged by size:
1393 792 778 552 370 190 135 92
80
Bsp1286I G_dGCh'C
Cuts at: 167 247 353 785 799 838 938 1169 1243
Size: 80 106 432 14 39 100 231 74
Cuts at: 1243 1261 1396 2007 2100 2174 2364 2426 3156
Size: 18 135 611 93 74 190 62 730
Cuts at: 3156 167
Size: 1393
Fragments arranged by size:
1393 730 611 432 231 190 135 106
100 93 80 74 74 62 39 18
14
BspEI T'CCGG_A
Cuts at: 944 3602 944
Size: 2658 1724
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 548 794 887 1432 1646 1948 2329 548
Size: 246 93 545 214 302 381 2601
Fragments arranged by size:
2601 545 381 302 246 214 93
BsrBI CCG'CTC
Cuts at: 50 1091 2674 2728 4212 4377 50
Size: 1041 1583 54 1484 165 55
Fragments arranged by size:
1583 1484 1041 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2294 2294
Size: 4382
BsrFI r'CCGG_y
Cuts at: 1232 2380 2561 2770 1232
Size: 1148 181 209 2844
Fragments arranged by size:
2844 1148 209 181
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 1008 1008
Size: 4382
BstYI r'GATC_y
Cuts at: 1 1407 1874 1897 2232 2478 3490 3576 3588
Size: 1406 467 23 335 246 1012 86 12
Cuts at: 3588 3934 1
Size: 346 449
Fragments arranged by size:
1406 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 120 208 224 722 951 1001 1061 1119 120
Size: 88 16 498 229 50 60 58 3383
Fragments arranged by size:
3383 498 229 88 60 58 50 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 648 2332 2513 2676 648
Size: 1684 181 163 2354
Fragments arranged by size:
2354 1684 181 163
BtsI GCAGTG_nn'
Cuts at: 1285 1625 1285
Size: 340 4042
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DraIII CAC_nnn'GTG
Cuts at: 705 705
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 126 512 638 743 998 1010 1967 2141 2532
Size: 386 126 105 255 12 957 174 391
Cuts at: 2532 2559 3904 126
Size: 27 1345 604
Fragments arranged by size:
1345 957 604 391 386 255 174 126
105 27 12
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 372 454 619 1120 2405 2615 372
Size: 82 165 501 1285 210 2139
Fragments arranged by size:
2139 1285 501 210 165 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1071 1324 2590 2900 3046 4192 1071
Size: 253 1266 310 146 1146 1261
Fragments arranged by size:
1266 1261 1146 310 253 146
EcoICRI GAG'CTC
Cuts at: 245 1259 1394 245
Size: 1014 135 3233
Fragments arranged by size:
3233 1014 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 365 430 703 904 1257 2207 2639 3386
Size: 193 65 273 201 353 950 432 747
Cuts at: 3386 3654 172
Size: 268 900
Fragments arranged by size:
950 900 747 432 353 273 268 201
193 65
EcoO109I rG'GnC_Cy
Cuts at: 338 349 350 1239 1521 338
Size: 11 1 889 282 3199
Fragments arranged by size:
3199 889 282 11 1
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 585 907 1287 1816 2064 3086 4065 585
Size: 322 380 529 248 1022 979 902
Fragments arranged by size:
1022 979 902 529 380 322 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1243 1275 2224 2254 2256 2286 1243
Size: 32 949 30 2 30 3339
Fragments arranged by size:
3339 949 32 30 30 2
HincII GTy'rAC
Cuts at: 609 1421 609
Size: 812 3570
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 509 3053 3237 509
Size: 2544 184 1654
Fragments arranged by size:
2544 1654 184
MscI TGG'CCA
Cuts at: 128 1000 2143 128
Size: 872 1143 2367
Fragments arranged by size:
2367 1143 872
MslI CAynn'nnrTG
Cuts at: 122 991 1006 2371 2498 122
Size: 869 15 1365 127 2006
Fragments arranged by size:
2006 1365 869 127 15
MspA1I CmG'CkG
Cuts at: 132 517 733 860 1493 1807 2167 3180 3425
Size: 385 216 127 633 314 360 1013 245
Cuts at: 3425 132
Size: 1089
Fragments arranged by size:
1089 1013 633 385 360 314 245 216
127
NaeI GCC'GGC
Cuts at: 1234 2563 1234
Size: 1329 3053
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 208 224 1001 1061 1119 208
Size: 16 777 60 58 3471
Fragments arranged by size:
3471 777 60 58 16
NgoMIV G'CCGG_C
Cuts at: 1232 2561 1232
Size: 1329 3053
NspI r_CATG'y
Cuts at: 1435 1935 2842 1435
Size: 500 907 2975
Fragments arranged by size:
2975 907 500
PasI CC'CwG_GG
Cuts at: 1371 1371
Size: 4382
PciI A'CATG_T
Cuts at: 2838 2838
Size: 4382
PflMI CCAn_nnn'nTGG
Cuts at: 1280 1280
Size: 4382
PfoI T'CCnGG_A
Cuts at: 1154 1154
Size: 4382
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PmlI CAC'GTG
Cuts at: 705 705
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 184 216 3521 3553 184
Size: 32 3305 32 1013
Fragments arranged by size:
3305 1013 32 32
PpuMI rG'GwC_Cy
Cuts at: 338 338
Size: 4382
PspOMI G'GGCC_C
Cuts at: 349 1239 349
Size: 890 3492
PstI C_TGCA'G
Cuts at: 175 1429 2114 175
Size: 1254 685 2443
Fragments arranged by size:
2443 1254 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 132 733 1807 2167 132
Size: 601 1074 360 2347
Fragments arranged by size:
2347 1074 601 360
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 247 1261 1396 247
Size: 1014 135 3233
Fragments arranged by size:
3233 1014 135
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 1120 2405 2615 1120
Size: 1285 210 2887
Fragments arranged by size:
2887 1285 210
SbfI CC_TGCA'GG
Cuts at: 175 1429 175
Size: 1254 3128
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SfcI C'TryA_G
Cuts at: 33 171 825 1425 2110 3103 3294 33
Size: 138 654 600 685 993 191 1121
Fragments arranged by size:
1121 993 685 654 600 191 138
SfiI GGCCn_nnn'nGGCC
Cuts at: 1238 1238
Size: 4382
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 739 1404 739
Size: 665 3717
SmlI C'TyrA_G
Cuts at: 1537 2452 2944 3206 1537
Size: 915 492 262 2713
Fragments arranged by size:
2713 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StuI AGG'CCT
Cuts at: 1297 1297
Size: 4382
StyI C'CwwG_G
Cuts at: 208 224 1001 1061 1119 1516 1870 208
Size: 16 777 60 58 397 354 2720
Fragments arranged by size:
2720 777 397 354 60 58 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 747 1965 2098 2557 2766 747
Size: 1218 133 459 209 2363
Fragments arranged by size:
2363 1218 459 209 133
TatI w'GTAC_w
Cuts at: 3897 3897
Size: 4382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 743 775 1100 1132 3899 3931 743
Size: 32 325 32 2767 32 1194
Fragments arranged by size:
2767 1194 325 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 222 993 1877 222
Size: 771 884 2727
Fragments arranged by size:
2727 884 771
XmaI C'CCGG_G
Cuts at: 737 1402 737
Size: 665 3717
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI