Restriction map for
pF1KE0709
(Circular) (Six-base) MAPSORT of: pF1KE0709.seq Check: 6501 from: 1 to: 3998
pF1KE0709 3998 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 323 1036 323
Size: 713 3285
Acc65I G'GTAC_C
Cuts at: 1014 1014
Size: 3998
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 623 1823 2255 3002 3270 430
Size: 193 1200 432 747 268 1158
Fragments arranged by size:
1200 1158 747 432 268 193
AflIII A'CryG_T
Cuts at: 488 1350 2454 3431 488
Size: 862 1104 977 1055
Fragments arranged by size:
1104 1055 977 862
AgeI A'CCGG_T
Cuts at: 2386 2386
Size: 3998
AlwNI CAG_nnn'CTG
Cuts at: 1303 2870 1303
Size: 1567 2431
ApaLI G'TGCA_C
Cuts at: 2768 2768
Size: 3998
ApoI r'AATT_y
Cuts at: 348 373 894 1002 3351 3607 348
Size: 25 521 108 2349 256 739
Fragments arranged by size:
2349 739 521 256 108 25
AseI AT'TA_AT
Cuts at: 20 205 20
Size: 185 3813
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 887 1018 887
Size: 131 3867
BamHI G'GATC_C
Cuts at: 1023 1023
Size: 3998
BanI G'GyrC_C
Cuts at: 1014 1428 1676 1711 1014
Size: 414 248 35 3301
Fragments arranged by size:
3301 414 248 35
BanII G_rGCy'C
Cuts at: 1012 2042 1012
Size: 1030 2968
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BbsI GAAGACnn'nnnn_
Cuts at: 418 418
Size: 3998
BciVI GTATCCnnnnn_n'
Cuts at: 214 1896 2657 3998 214
Size: 1682 761 1341 214
Fragments arranged by size:
1682 1341 761 214
BclI T'GATC_A
Cuts at: 1518 1518
Size: 3998
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3998
BglII A'GATC_T
Cuts at: 1513 3550 1513
Size: 2037 1961
BlpI GC'TnA_GC
Cuts at: 1110 1110
Size: 3998
Bme1580I G_kGCm'C
Cuts at: 1623 1716 2772 1623
Size: 93 1056 2849
Fragments arranged by size:
2849 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 938 1210 1291 1626 3770 938
Size: 272 81 335 2144 1166
Fragments arranged by size:
2144 1166 335 272 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 3998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 664
Size: 3998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 2053 2545 2843 1174
Size: 879 492 298 2329
Fragments arranged by size:
2329 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3702 3702
Size: 3998
BsaAI yAC'GTr
Cuts at: 1351 1351
Size: 3998
BsaBI GATnn'nnATC
Cuts at: 6 1512 3549 6
Size: 1506 2037 455
Fragments arranged by size:
2037 1506 455
BsaHI Gr'CG_yC
Cuts at: 1429 1677 2379 3875 1429
Size: 248 702 1496 1552
Fragments arranged by size:
1552 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1187 1708 2386 2660 2807 3218 1187
Size: 521 678 274 147 411 1967
Fragments arranged by size:
1967 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1423 2181 2758 3254 1423
Size: 758 577 496 2167
Fragments arranged by size:
2167 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1540 1586 2794 104
Size: 1436 46 1208 1308
Fragments arranged by size:
1436 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1012 1790 1980 2772 1012
Size: 778 190 792 2238
Fragments arranged by size:
2238 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1012 1623 1716 1790 1980 2042 2772 1012
Size: 611 93 74 190 62 730 2238
Fragments arranged by size:
2238 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3218 3218
Size: 3998
BspHI T'CATG_A
Cuts at: 3174 3174
Size: 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1048 1262 1564 1945 1048
Size: 214 302 381 3101
Fragments arranged by size:
3101 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2290 2344 3828 3993 50
Size: 2240 54 1484 165 55
Fragments arranged by size:
2240 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 986 1910 986
Size: 924 3074
BsrFI r'CCGG_y
Cuts at: 1996 2177 2386 1996
Size: 181 209 3608
Fragments arranged by size:
3608 209 181
BssSI C'ACGA_G
Cuts at: 2269 2627 2269
Size: 358 3640
BstAPI GCAn_nnn'nTGC
Cuts at: 1375 1375
Size: 3998
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstYI r'GATC_y
Cuts at: 1 146 1023 1490 1513 1848 2094 3106 3192
Size: 145 877 467 23 335 246 1012 86
Cuts at: 3192 3204 3550 1
Size: 12 346 449
Fragments arranged by size:
1012 877 467 449 346 335 246 145
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 712 712
Size: 3998
BtgI C'CryG_G
Cuts at: 578 578
Size: 3998
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 1241 1241
Size: 3998
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 2562 1704
Size: 858 3140
EaeI y'GGCC_r
Cuts at: 1583 1757 2148 2175 3520 1583
Size: 174 391 27 1345 2061
Fragments arranged by size:
2061 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1583 1583
Size: 3998
EarI CTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2206 2516 2662 3808 2206
Size: 310 146 1146 2396
Fragments arranged by size:
2396 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1010 1010
Size: 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 623 664 1823 2255 3002 3270 430
Size: 193 41 1159 432 747 268 1158
Fragments arranged by size:
1159 1158 747 432 268 193 41
EcoO109I rG'GnC_Cy
Cuts at: 1137 1137
Size: 3998
EcoRI G'AATT_C
Cuts at: 348 1002 348
Size: 654 3344
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3998
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 1432 1680 2702 3681 1432
Size: 248 1022 979 1749
Fragments arranged by size:
1749 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 159 191 1840 1870 1872 1902 159
Size: 32 1649 30 2 30 2255
Fragments arranged by size:
2255 1649 32 30 30 2
HincII GTy'rAC
Cuts at: 306 1037 306
Size: 731 3267
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 1018 1018
Size: 3998
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2669 2853 2669
Size: 184 3814
MscI TGG'CCA
Cuts at: 1759 1759
Size: 3998
MslI CAynn'nnrTG
Cuts at: 909 1987 2114 909
Size: 1078 127 2793
Fragments arranged by size:
2793 1078 127
MspA1I CmG'CkG
Cuts at: 1109 1423 1783 2796 3041 1109
Size: 314 360 1013 245 2066
Fragments arranged by size:
2066 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2179 2179
Size: 3998
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NgoMIV G'CCGG_C
Cuts at: 2177 2177
Size: 3998
NspI r_CATG'y
Cuts at: 335 641 1051 1551 2458 335
Size: 306 410 500 907 1875
Fragments arranged by size:
1875 907 500 410 306
PciI A'CATG_T
Cuts at: 2454 2454
Size: 3998
PfoI T'CCnGG_A
Cuts at: 152 655 152
Size: 503 3495
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3966
PspXI vC'TCGA_Gb
Cuts at: 887 887
Size: 3998
PstI C_TGCA'G
Cuts at: 1045 1730 1045
Size: 685 3313
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 1423 1783 1423
Size: 360 3638
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 1012 1012
Size: 3998
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 3515 3515
Size: 3998
SfcI C'TryA_G
Cuts at: 33 1041 1726 2719 2910 33
Size: 1008 685 993 191 1121
Fragments arranged by size:
1121 1008 993 685 191
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 1020 1020
Size: 3998
SmlI C'TyrA_G
Cuts at: 887 1153 2068 2560 2822 887
Size: 266 915 492 262 2063
Fragments arranged by size:
2063 915 492 266 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 1051 1551 1051
Size: 500 3498
StuI AGG'CCT
Cuts at: 717 717
Size: 3998
StyI C'CwwG_G
Cuts at: 1132 1486 1132
Size: 354 3644
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1581 1714 2173 2382 1581
Size: 133 459 209 3197
Fragments arranged by size:
3197 459 209 133
TatI w'GTAC_w
Cuts at: 185 402 618 3513 185
Size: 217 216 2895 670
Fragments arranged by size:
2895 670 217 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 3966
Tth111I GACn'n_nGTC
Cuts at: 1795 1795
Size: 3998
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1493 1493
Size: 3998
XhoI C'TCGA_G
Cuts at: 887 887
Size: 3998
XmaI C'CCGG_G
Cuts at: 1018 1018
Size: 3998
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI