Restriction map for
pF1KE0708
(Circular) (Six-base) MAPSORT of: pF1KE0708.seq Check: 2215 from: 1 to: 4334
pF1KE0708 4334 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 346 346
Size: 4334
AccI GT'mk_AC
Cuts at: 1372 1372
Size: 4334
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 855 2159 2591 3338 3606 603
Size: 252 1304 432 747 268 1331
Fragments arranged by size:
1331 1304 747 432 268 252
AflIII A'CryG_T
Cuts at: 913 1686 2790 3767 913
Size: 773 1104 977 1480
Fragments arranged by size:
1480 1104 977 773
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AleI CACnn'nnGTG
Cuts at: 383 467 383
Size: 84 4250
AlwNI CAG_nnn'CTG
Cuts at: 459 1639 3206 459
Size: 1180 1567 1587
Fragments arranged by size:
1587 1567 1180
ApaLI G'TGCA_C
Cuts at: 3104 3104
Size: 4334
ApoI r'AATT_y
Cuts at: 172 971 1015 1270 1338 3687 3943 172
Size: 799 44 255 68 2349 256 563
Fragments arranged by size:
2349 799 563 256 255 68 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 4334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 1354 1354
Size: 4334
BamHI G'GATC_C
Cuts at: 1359 1359
Size: 4334
BanI G'GyrC_C
Cuts at: 297 1350 1764 2012 2047 297
Size: 1053 414 248 35 2584
Fragments arranged by size:
2584 1053 414 248 35
BanII G_rGCy'C
Cuts at: 1348 2378 1348
Size: 1030 3304
BbeI G_GCGC'C
Cuts at: 1768 2016 1768
Size: 248 4086
BbsI GAAGACnn'nnnn_
Cuts at: 164 609 164
Size: 445 3889
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 1854 1854
Size: 4334
BfrBI ATG'CAT
Cuts at: 758 758
Size: 4334
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4334
BglII A'GATC_T
Cuts at: 1849 3886 1849
Size: 2037 2297
BlpI GC'TnA_GC
Cuts at: 1446 1446
Size: 4334
Bme1580I G_kGCm'C
Cuts at: 145 1959 2052 3108 145
Size: 1814 93 1056 1371
Fragments arranged by size:
1814 1371 1056 93
BmgBI CAC'GTC
Cuts at: 162 162
Size: 4334
BmrI ACTGGGnnnn_n'
Cuts at: 692 1546 1627 1962 4106 692
Size: 854 81 335 2144 920
Fragments arranged by size:
2144 920 854 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 646 1001 1085 1185 1253 259
Size: 387 355 84 100 68 3340
Fragments arranged by size:
3340 387 355 100 84 68
Bpu10I CC'TnA_GC
Cuts at: 124 646 124
Size: 522 3812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 2389 2881 3179 1510
Size: 879 492 298 2665
Fragments arranged by size:
2665 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4038 4038
Size: 4334
BsaAI yAC'GTr
Cuts at: 914 1687 914
Size: 773 3561
BsaBI GATnn'nnATC
Cuts at: 6 1848 3885 6
Size: 1842 2037 455
Fragments arranged by size:
2037 1842 455
BsaHI Gr'CG_yC
Cuts at: 1765 2013 2715 4211 1765
Size: 248 702 1496 1888
Fragments arranged by size:
1888 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1523 2044 2722 2996 3143 3554 1523
Size: 521 678 274 147 411 2303
Fragments arranged by size:
2303 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 556 586 556
Size: 30 4304
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 577 577
Size: 4334
BseYI C'CCAG_C
Cuts at: 1759 2517 3094 3590 1759
Size: 758 577 496 2503
Fragments arranged by size:
2503 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1188 1272 445
Size: 743 84 3507
Fragments arranged by size:
3507 743 84
BsiEI CG_ry'CG
Cuts at: 104 119 1876 1922 3130 104
Size: 15 1757 46 1208 1308
Fragments arranged by size:
1757 1308 1208 46 15
BsiHKAI G_wGCw'C
Cuts at: 530 1348 2126 2316 3108 530
Size: 818 778 190 792 1756
Fragments arranged by size:
1756 818 792 778 190
BsmI GAATG_Cn'
Cuts at: 1087 1087
Size: 4334
Bsp1286I G_dGCh'C
Cuts at: 145 530 1348 1959 2052 2126 2316 2378 3108
Size: 385 818 611 93 74 190 62 730
Cuts at: 3108 145
Size: 1371
Fragments arranged by size:
1371 818 730 611 385 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3554 3554
Size: 4334
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 346 1384 1598 1900 2281 346
Size: 1038 214 302 381 2399
Fragments arranged by size:
2399 1038 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2626 2680 4164 4329 50
Size: 2576 54 1484 165 55
Fragments arranged by size:
2576 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1040 2246 1040
Size: 1206 3128
BsrFI r'CCGG_y
Cuts at: 2332 2513 2722 2332
Size: 181 209 3944
Fragments arranged by size:
3944 209 181
BsrGI T'GTAC_A
Cuts at: 960 960
Size: 4334
BssSI C'ACGA_G
Cuts at: 796 2605 2963 796
Size: 1809 358 2167
Fragments arranged by size:
2167 1809 358
BstAPI GCAn_nnn'nTGC
Cuts at: 459 1711 459
Size: 1252 3082
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstYI r'GATC_y
Cuts at: 1 1359 1826 1849 2184 2430 3442 3528 3540
Size: 1358 467 23 335 246 1012 86 12
Cuts at: 3540 3886 1
Size: 346 449
Fragments arranged by size:
1358 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2284 2465 2628 2284
Size: 181 163 3990
Fragments arranged by size:
3990 181 163
BtsI GCAGTG_nn'
Cuts at: 1092 1176 1260 1577 1092
Size: 84 84 317 3849
Fragments arranged by size:
3849 317 84 84
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 2040 2898 2040
Size: 858 3476
EaeI y'GGCC_r
Cuts at: 116 1919 2093 2484 2511 3856 116
Size: 1803 174 391 27 1345 594
Fragments arranged by size:
1803 1345 594 391 174 27
EagI C'GGCC_G
Cuts at: 116 1919 116
Size: 1803 2531
EarI CTCTTCn'nnn_
Cuts at: 574 1290 2357 2567 574
Size: 716 1067 210 2341
Fragments arranged by size:
2341 1067 716 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2542 2852 2998 4144 2542
Size: 310 146 1146 2732
Fragments arranged by size:
2732 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1346 1346
Size: 4334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 603 646 855 1001 1085 1185 1253 2159
Size: 344 43 209 146 84 100 68 906
Cuts at: 2159 2591 3338 3606 259
Size: 432 747 268 987
Fragments arranged by size:
987 906 747 432 344 268 209 146
100 84 68 43
EcoNI CCTnn'n_nnAGG
Cuts at: 202 353 202
Size: 151 4183
EcoO109I rG'GnC_Cy
Cuts at: 1473 1473
Size: 4334
EcoRI G'AATT_C
Cuts at: 971 1338 971
Size: 367 3967
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 4334
FspI TGC'GCA
Cuts at: 2115 2115
Size: 4334
HaeII r_GCGC'y
Cuts at: 1768 2016 3038 4017 1768
Size: 248 1022 979 2085
Fragments arranged by size:
2085 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 697 729 2176 2206 2208 2238 697
Size: 32 1447 30 2 30 2793
Fragments arranged by size:
2793 1447 32 30 30 2
HincII GTy'rAC
Cuts at: 1373 1373
Size: 4334
KasI G'GCGC_C
Cuts at: 1764 2012 1764
Size: 248 4086
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3005 3189 3005
Size: 184 4150
MscI TGG'CCA
Cuts at: 2095 2095
Size: 4334
MslI CAynn'nnrTG
Cuts at: 383 467 521 996 1080 2323 2450 383
Size: 84 54 475 84 1243 127 2267
Fragments arranged by size:
2267 1243 475 127 84 84 54
MspA1I CmG'CkG
Cuts at: 746 1445 1759 2119 3132 3377 746
Size: 699 314 360 1013 245 1703
Fragments arranged by size:
1703 1013 699 360 314 245
NaeI GCC'GGC
Cuts at: 2515 2515
Size: 4334
NarI GG'CG_CC
Cuts at: 1765 2013 1765
Size: 248 4086
NcoI C'CATG_G
Cuts at: 107 107
Size: 4334
NdeI CA'TA_TG
Cuts at: 993 1077 993
Size: 84 4250
NgoMIV G'CCGG_C
Cuts at: 2513 2513
Size: 4334
NotI GC'GGCC_GC
Cuts at: 116 116
Size: 4334
NsiI A_TGCA'T
Cuts at: 760 760
Size: 4334
NspI r_CATG'y
Cuts at: 424 598 1387 1887 2794 424
Size: 174 789 500 907 1964
Fragments arranged by size:
1964 907 789 500 174
PasI CC'CwG_GG
Cuts at: 264 264
Size: 4334
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PshAI GACnn'nnGTC
Cuts at: 337 337
Size: 4334
PstI C_TGCA'G
Cuts at: 1381 2066 1381
Size: 685 3649
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 746 1759 2119 746
Size: 1013 360 2961
Fragments arranged by size:
2961 1013 360
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 1348 1348
Size: 4334
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 3851 3851
Size: 4334
SfcI C'TryA_G
Cuts at: 33 546 812 1377 2062 3055 3246 33
Size: 513 266 565 685 993 191 1121
Fragments arranged by size:
1121 993 685 565 513 266 191
SfoI GGC'GCC
Cuts at: 1766 2014 1766
Size: 248 4086
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SmaI CCC'GGG
Cuts at: 1356 1356
Size: 4334
SmlI C'TyrA_G
Cuts at: 1489 2404 2896 3158 1489
Size: 915 492 262 2665
Fragments arranged by size:
2665 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 1387 1887 1387
Size: 500 3834
StuI AGG'CCT
Cuts at: 475 475
Size: 4334
StyI C'CwwG_G
Cuts at: 107 1468 1822 107
Size: 1361 354 2619
Fragments arranged by size:
2619 1361 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1917 2050 2509 2718 1917
Size: 133 459 209 3533
Fragments arranged by size:
3533 459 209 133
TatI w'GTAC_w
Cuts at: 960 3849 960
Size: 2889 1445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4302
Tth111I GACn'n_nGTC
Cuts at: 2131 2131
Size: 4334
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 745 1829 745
Size: 1084 3250
XmaI C'CCGG_G
Cuts at: 1354 1354
Size: 4334
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI
NsiI NspI PasI PciI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BmtI BsiWI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NruI PacI
PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI