Restriction map for
pF1KE0707
(Circular) (Six-base) MAPSORT of: pF1KE0707.seq Check: 617 from: 1 to: 4391
pF1KE0707 4391 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 961 961
Size: 4391
AccI GT'mk_AC
Cuts at: 1429 1429
Size: 4391
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 2216 2648 3395 3663 390
Size: 1826 432 747 268 1118
Fragments arranged by size:
1826 1118 747 432 268
AflIII A'CryG_T
Cuts at: 1743 2847 3824 1743
Size: 1104 977 2310
Fragments arranged by size:
2310 1104 977
AgeI A'CCGG_T
Cuts at: 2779 2779
Size: 4391
AlwNI CAG_nnn'CTG
Cuts at: 1696 3263 1696
Size: 1567 2824
ApaI G_GGCC'C
Cuts at: 269 636 742 786 1041 269
Size: 367 106 44 255 3619
Fragments arranged by size:
3619 367 255 106 44
ApaLI G'TGCA_C
Cuts at: 598 1117 3161 598
Size: 519 2044 1828
Fragments arranged by size:
2044 1828 519
ApoI r'AATT_y
Cuts at: 1395 3744 4000 1395
Size: 2349 256 1786
Fragments arranged by size:
2349 1786 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 1225 1411 1225
Size: 186 4205
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 241 274 241
Size: 33 4358
BamHI G'GATC_C
Cuts at: 1416 1416
Size: 4391
BanI G'GyrC_C
Cuts at: 1407 1821 2069 2104 1407
Size: 414 248 35 3694
Fragments arranged by size:
3694 414 248 35
BanII G_rGCy'C
Cuts at: 269 461 636 742 786 928 1041 1153 1315
Size: 192 175 106 44 142 113 112 162
Cuts at: 1315 1405 2435 269
Size: 90 1030 2225
Fragments arranged by size:
2225 1030 192 175 162 142 113 112
106 90 44
BbeI G_GCGC'C
Cuts at: 1825 2073 1825
Size: 248 4143
BbvCI CC'TCA_GC
Cuts at: 1273 1273
Size: 4391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 955 989 955
Size: 34 4357
BciVI GTATCCnnnnn_n'
Cuts at: 2289 3050 4391 2289
Size: 761 1341 2289
Fragments arranged by size:
2289 1341 761
BclI T'GATC_A
Cuts at: 1911 1911
Size: 4391
BglI GCCn_nnn'nGGC
Cuts at: 707 707
Size: 4391
BglII A'GATC_T
Cuts at: 1906 3943 1906
Size: 2037 2354
BlpI GC'TnA_GC
Cuts at: 1503 1503
Size: 4391
Bme1580I G_kGCm'C
Cuts at: 209 269 602 636 731 742 786 1041 1121
Size: 60 333 34 95 11 44 255 80
Cuts at: 1121 2016 2109 3165 209
Size: 895 93 1056 1435
Fragments arranged by size:
1435 1056 895 333 255 95 93 80
60 44 34 11
BmgBI CAC'GTC
Cuts at: 1122 1122
Size: 4391
BmrI ACTGGGnnnn_n'
Cuts at: 736 1603 1684 2019 4163 736
Size: 867 81 335 2144 964
Fragments arranged by size:
2144 964 867 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 4391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 475 592 946 164
Size: 311 117 354 3609
Fragments arranged by size:
3609 354 311 117
Bpu10I CC'TnA_GC
Cuts at: 571 1273 571
Size: 702 3689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 1567 2446 2938 3236 238
Size: 1329 879 492 298 1393
Fragments arranged by size:
1393 1329 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 140 1195 1366 4095 140
Size: 1055 171 2729 436
Fragments arranged by size:
2729 1055 436 171
BsaAI yAC'GTr
Cuts at: 726 1116 1744 726
Size: 390 628 3373
Fragments arranged by size:
3373 628 390
BsaBI GATnn'nnATC
Cuts at: 6 1905 3942 6
Size: 1899 2037 455
Fragments arranged by size:
2037 1899 455
BsaHI Gr'CG_yC
Cuts at: 1822 2070 2772 4268 1822
Size: 248 702 1496 1945
Fragments arranged by size:
1945 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1580 2101 2779 3053 3200 3611 1580
Size: 521 678 274 147 411 2360
Fragments arranged by size:
2360 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1111 1141 1204 1234 1111
Size: 30 63 30 4268
Fragments arranged by size:
4268 63 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 104 104
Size: 4391
BseYI C'CCAG_C
Cuts at: 269 492 1034 1325 1342 1816 2574 3151 3647
Size: 223 542 291 17 474 758 577 496
Cuts at: 3647 269
Size: 1013
Fragments arranged by size:
1013 758 577 542 496 474 291 223
17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 706 823 1081 688
Size: 18 117 258 3998
Fragments arranged by size:
3998 258 117 18
BsiEI CG_ry'CG
Cuts at: 104 975 1056 1933 1979 3187 104
Size: 871 81 877 46 1208 1308
Fragments arranged by size:
1308 1208 877 871 81 46
BsiHKAI G_wGCw'C
Cuts at: 461 557 602 857 1121 1172 1405 2183 2373
Size: 96 45 255 264 51 233 778 190
Cuts at: 2373 3165 461
Size: 792 1687
Fragments arranged by size:
1687 792 778 264 255 233 190 96
51 45
Bsp1286I G_dGCh'C
Cuts at: 209 269 461 557 602 636 731 742 786
Size: 60 192 96 45 34 95 11 44
Cuts at: 786 857 928 1041 1121 1153 1172 1315 1405
Size: 71 71 113 80 32 19 143 90
Cuts at: 1405 2016 2109 2183 2373 2435 3165 209
Size: 611 93 74 190 62 730 1435
Fragments arranged by size:
1435 730 611 192 190 143 113 96
95 93 90 80 74 71 71 62
60 45 44 34 32 19 11
BspEI T'CCGG_A
Cuts at: 3611 3611
Size: 4391
BspHI T'CATG_A
Cuts at: 845 3567 845
Size: 2722 1669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 317 434 869 961 1441 1655 1957 2338 317
Size: 117 435 92 480 214 302 381 2370
Fragments arranged by size:
2370 480 435 381 302 214 117 92
BsrBI CCG'CTC
Cuts at: 50 2683 2737 4221 4386 50
Size: 2633 54 1484 165 55
Fragments arranged by size:
2633 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 192 345 2303 192
Size: 153 1958 2280
Fragments arranged by size:
2280 1958 153
BsrFI r'CCGG_y
Cuts at: 2389 2570 2779 2389
Size: 181 209 4001
Fragments arranged by size:
4001 209 181
BssSI C'ACGA_G
Cuts at: 2662 3020 2662
Size: 358 4033
BstAPI GCAn_nnn'nTGC
Cuts at: 1768 1768
Size: 4391
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 621 621
Size: 4391
BstYI r'GATC_y
Cuts at: 1 1416 1883 1906 2241 2487 3499 3585 3597
Size: 1415 467 23 335 246 1012 86 12
Cuts at: 3597 3943 1
Size: 346 449
Fragments arranged by size:
1415 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 218 218
Size: 4391
BtgI C'CryG_G
Cuts at: 176 593 620 778 836 176
Size: 417 27 158 58 3731
Fragments arranged by size:
3731 417 158 58 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1225 2341 2522 2685 1225
Size: 1116 181 163 2931
Fragments arranged by size:
2931 1116 181 163
BtsI GCAGTG_nn'
Cuts at: 198 563 1634 198
Size: 365 1071 2955
Fragments arranged by size:
2955 1071 365
DraI TTT'AAA
Cuts at: 1390 1390
Size: 4391
DraIII CAC_nnn'GTG
Cuts at: 1301 1301
Size: 4391
DrdI GACnn_nn'nnGTC
Cuts at: 2097 2955 2097
Size: 858 3533
EaeI y'GGCC_r
Cuts at: 197 413 590 839 1157 1976 2150 2541 2568
Size: 216 177 249 318 819 174 391 27
Cuts at: 2568 3913 197
Size: 1345 675
Fragments arranged by size:
1345 819 675 391 318 249 216 177
174 27
EagI C'GGCC_G
Cuts at: 1976 1976
Size: 4391
EarI CTCTTCn'nnn_
Cuts at: 409 826 2414 2624 409
Size: 417 1588 210 2176
Fragments arranged by size:
2176 1588 417 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 763 2599 2909 3055 4201 763
Size: 1836 310 146 1146 953
Fragments arranged by size:
1836 1146 953 310 146
EcoICRI GAG'CTC
Cuts at: 459 1403 459
Size: 944 3447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 390 475 592 946 2216 2648 3395 3663
Size: 226 85 117 354 1270 432 747 268
Cuts at: 3663 164
Size: 892
Fragments arranged by size:
1270 892 747 432 354 268 226 117
85
EcoO109I rG'GnC_Cy
Cuts at: 265 266 738 783 1038 1215 1229 1530 265
Size: 1 472 45 255 177 14 301 3126
Fragments arranged by size:
3126 472 301 255 177 45 14 1
EcoRI G'AATT_C
Cuts at: 1395 1395
Size: 4391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4391
FspI TGC'GCA
Cuts at: 382 2172 382
Size: 1790 2601
FspAI rTGC'GCAy
Cuts at: 382 382
Size: 4391
HaeII r_GCGC'y
Cuts at: 332 1825 2073 3095 4074 332
Size: 1493 248 1022 979 649
Fragments arranged by size:
1493 1022 979 649 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1297 1329 2233 2263 2265 2295 1297
Size: 32 904 30 2 30 3393
Fragments arranged by size:
3393 904 32 30 30 2
HincII GTy'rAC
Cuts at: 1430 1430
Size: 4391
KasI G'GCGC_C
Cuts at: 1821 2069 1821
Size: 248 4143
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MfeI C'AATT_G
Cuts at: 126 126
Size: 4391
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 3062 3246 401
Size: 2661 184 1546
Fragments arranged by size:
2661 1546 184
MscI TGG'CCA
Cuts at: 841 2152 841
Size: 1311 3080
MslI CAynn'nnrTG
Cuts at: 799 850 2380 2507 799
Size: 51 1530 127 2683
Fragments arranged by size:
2683 1530 127 51
MspA1I CmG'CkG
Cuts at: 720 757 1325 1502 1816 2176 3189 3434 720
Size: 37 568 177 314 360 1013 245 1677
Fragments arranged by size:
1677 1013 568 360 314 245 177 37
NaeI GCC'GGC
Cuts at: 2572 2572
Size: 4391
NarI GG'CG_CC
Cuts at: 1822 2070 1822
Size: 248 4143
NcoI C'CATG_G
Cuts at: 176 620 836 176
Size: 444 216 3731
Fragments arranged by size:
3731 444 216
NgoMIV G'CCGG_C
Cuts at: 2570 2570
Size: 4391
NspI r_CATG'y
Cuts at: 768 1444 1944 2851 768
Size: 676 500 907 2308
Fragments arranged by size:
2308 907 676 500
PasI CC'CwG_GG
Cuts at: 157 1317 157
Size: 1160 3231
PciI A'CATG_T
Cuts at: 2847 2847
Size: 4391
PflMI CCAn_nnn'nTGG
Cuts at: 1338 1338
Size: 4391
PfoI T'CCnGG_A
Cuts at: 1208 1208
Size: 4391
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PmlI CAC'GTG
Cuts at: 726 1116 726
Size: 390 4001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 4359
PspOMI G'GGCC_C
Cuts at: 265 632 738 782 1037 265
Size: 367 106 44 255 3619
Fragments arranged by size:
3619 367 255 106 44
PstI C_TGCA'G
Cuts at: 933 958 1438 2123 933
Size: 25 480 685 3201
Fragments arranged by size:
3201 685 480 25
PvuI CG_AT'CG
Cuts at: 104 975 104
Size: 871 3520
PvuII CAG'CTG
Cuts at: 720 757 1325 1816 2176 720
Size: 37 568 491 360 2935
Fragments arranged by size:
2935 568 491 360 37
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 461 1405 461
Size: 944 3447
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 826 2414 2624 826
Size: 1588 210 2593
Fragments arranged by size:
2593 1588 210
SbfI CC_TGCA'GG
Cuts at: 958 1438 958
Size: 480 3911
ScaI AGT'ACT
Cuts at: 3908 3908
Size: 4391
SfcI C'TryA_G
Cuts at: 33 929 954 1173 1434 2119 3112 3303 33
Size: 896 25 219 261 685 993 191 1121
Fragments arranged by size:
1121 993 896 685 261 219 191 25
SfoI GGC'GCC
Cuts at: 1823 2071 1823
Size: 248 4143
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SmaI CCC'GGG
Cuts at: 1413 1413
Size: 4391
SmlI C'TyrA_G
Cuts at: 253 1546 2461 2953 3215 253
Size: 1293 915 492 262 1429
Fragments arranged by size:
1429 1293 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 768 1444 1944 768
Size: 676 500 3215
Fragments arranged by size:
3215 676 500
StyI C'CwwG_G
Cuts at: 176 620 836 914 1525 1879 176
Size: 444 216 78 611 354 2688
Fragments arranged by size:
2688 611 444 354 216 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 617 1390 1974 2107 2566 2775 617
Size: 773 584 133 459 209 2233
Fragments arranged by size:
2233 773 584 459 209 133
TatI w'GTAC_w
Cuts at: 3906 3906
Size: 4391
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1111 1143 1204 1236 3908 3940 1111
Size: 32 61 32 2672 32 1562
Fragments arranged by size:
2672 1562 61 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1057 2188 1057
Size: 1131 3260
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1339 1886 1339
Size: 547 3844
XmaI C'CCGG_G
Cuts at: 1411 1411
Size: 4391
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbsI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI