Restriction map for
pF1KE0703
(Circular) (Six-base) MAPSORT of: pF1KE0703.seq Check: 5492 from: 1 to: 3992
pF1KE0703 3992 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 718 718
Size: 3992
AccI GT'mk_AC
Cuts at: 718 1030 718
Size: 312 3680
Acc65I G'GTAC_C
Cuts at: 1008 1008
Size: 3992
AclI AA'CG_TT
Cuts at: 3832 3832
Size: 3992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1817 2249 2996 3264 1817
Size: 432 747 268 2545
Fragments arranged by size:
2545 747 432 268
AflIII A'CryG_T
Cuts at: 1344 2448 3425 1344
Size: 1104 977 1911
Fragments arranged by size:
1911 1104 977
AgeI A'CCGG_T
Cuts at: 2380 2380
Size: 3992
AlwNI CAG_nnn'CTG
Cuts at: 1297 2864 1297
Size: 1567 2425
ApaI G_GGCC'C
Cuts at: 805 805
Size: 3992
ApaLI G'TGCA_C
Cuts at: 2762 2762
Size: 3992
ApoI r'AATT_y
Cuts at: 996 3345 3601 996
Size: 2349 256 1387
Fragments arranged by size:
2349 1387 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
AvaI C'yCGr_G
Cuts at: 1012 1012
Size: 3992
BamHI G'GATC_C
Cuts at: 1017 1017
Size: 3992
BanI G'GyrC_C
Cuts at: 1008 1422 1670 1705 1008
Size: 414 248 35 3295
Fragments arranged by size:
3295 414 248 35
BanII G_rGCy'C
Cuts at: 805 1006 2036 805
Size: 201 1030 2761
Fragments arranged by size:
2761 1030 201
BbeI G_GCGC'C
Cuts at: 1426 1674 1426
Size: 248 3744
BbsI GAAGACnn'nnnn_
Cuts at: 790 790
Size: 3992
BciVI GTATCCnnnnn_n'
Cuts at: 1890 2651 3992 1890
Size: 761 1341 1890
Fragments arranged by size:
1890 1341 761
BclI T'GATC_A
Cuts at: 1512 1512
Size: 3992
BfrBI ATG'CAT
Cuts at: 373 373
Size: 3992
BglII A'GATC_T
Cuts at: 287 1507 3544 287
Size: 1220 2037 735
Fragments arranged by size:
2037 1220 735
BlpI GC'TnA_GC
Cuts at: 1104 1104
Size: 3992
Bme1580I G_kGCm'C
Cuts at: 805 1617 1710 2766 805
Size: 812 93 1056 2031
Fragments arranged by size:
2031 1056 812 93
BmrI ACTGGGnnnn_n'
Cuts at: 300 1204 1285 1620 3764 300
Size: 904 81 335 2144 528
Fragments arranged by size:
2144 904 528 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 3992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 260
Size: 3992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 1168 2047 2539 2837 833
Size: 335 879 492 298 1988
Fragments arranged by size:
1988 879 492 335 298
BsaI GGTCTCn'nnnn_
Cuts at: 3696 3696
Size: 3992
BsaAI yAC'GTr
Cuts at: 739 1345 739
Size: 606 3386
BsaBI GATnn'nnATC
Cuts at: 6 1506 3543 6
Size: 1500 2037 455
Fragments arranged by size:
2037 1500 455
BsaHI Gr'CG_yC
Cuts at: 715 1423 1671 2373 3869 715
Size: 708 248 702 1496 838
Fragments arranged by size:
1496 838 708 702 248
BsaWI w'CCGG_w
Cuts at: 1181 1702 2380 2654 2801 3212 1181
Size: 521 678 274 147 411 1961
Fragments arranged by size:
1961 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 373
Size: 30 3962
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 607 607
Size: 3992
BseYI C'CCAG_C
Cuts at: 130 209 1417 2175 2752 3248 130
Size: 79 1208 758 577 496 874
Fragments arranged by size:
1208 874 758 577 496 79
BsiEI CG_ry'CG
Cuts at: 104 1534 1580 2788 104
Size: 1430 46 1208 1308
Fragments arranged by size:
1430 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1006 1784 1974 2766 1006
Size: 778 190 792 2232
Fragments arranged by size:
2232 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 805 1006 1617 1710 1784 1974 2036 2766 805
Size: 201 611 93 74 190 62 730 2031
Fragments arranged by size:
2031 730 611 201 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3212 3212
Size: 3992
BspHI T'CATG_A
Cuts at: 3168 3168
Size: 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 180 1042 1256 1558 1939 180
Size: 862 214 302 381 2233
Fragments arranged by size:
2233 862 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2284 2338 3822 3987 50
Size: 2234 54 1484 165 55
Fragments arranged by size:
2234 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1904 1904
Size: 3992
BsrFI r'CCGG_y
Cuts at: 1990 2171 2380 1990
Size: 181 209 3602
Fragments arranged by size:
3602 209 181
BsrGI T'GTAC_A
Cuts at: 817 817
Size: 3992
BssSI C'ACGA_G
Cuts at: 2263 2621 2263
Size: 358 3634
BstAPI GCAn_nnn'nTGC
Cuts at: 1369 1369
Size: 3992
BstBI TT'CG_AA
Cuts at: 2353 2353
Size: 3992
BstYI r'GATC_y
Cuts at: 1 287 1017 1484 1507 1842 2088 3100 3186
Size: 286 730 467 23 335 246 1012 86
Cuts at: 3186 3198 3544 1
Size: 12 346 449
Fragments arranged by size:
1012 730 467 449 346 335 286 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3992
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1942 2123 2286 1942
Size: 181 163 3648
Fragments arranged by size:
3648 181 163
BtsI GCAGTG_nn'
Cuts at: 1235 1235
Size: 3992
DraI TTT'AAA
Cuts at: 501 603 991 501
Size: 102 388 3502
Fragments arranged by size:
3502 388 102
DrdI GACnn_nn'nnGTC
Cuts at: 1698 2556 1698
Size: 858 3134
EaeI y'GGCC_r
Cuts at: 1577 1751 2142 2169 3514 1577
Size: 174 391 27 1345 2055
Fragments arranged by size:
2055 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1577 1577
Size: 3992
EarI CTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2200 2510 2656 3802 2200
Size: 310 146 1146 2390
Fragments arranged by size:
2390 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1004 1004
Size: 3992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 1817 2249 2996 3264 260
Size: 1557 432 747 268 988
Fragments arranged by size:
1557 988 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 801 802 1131 801
Size: 1 329 3662
Fragments arranged by size:
3662 329 1
EcoRI G'AATT_C
Cuts at: 996 996
Size: 3992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 247 2011 247
Size: 1764 2228
FspI TGC'GCA
Cuts at: 1773 1773
Size: 3992
HaeII r_GCGC'y
Cuts at: 1426 1674 2696 3675 1426
Size: 248 1022 979 1743
Fragments arranged by size:
1743 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 1834 1864 1866 1896 373
Size: 32 1429 30 2 30 2469
Fragments arranged by size:
2469 1429 32 30 30 2
HincII GTy'rAC
Cuts at: 1031 1031
Size: 3992
KasI G'GCGC_C
Cuts at: 1422 1670 1422
Size: 248 3744
KpnI G_GTAC'C
Cuts at: 1012 1012
Size: 3992
MluI A'CGCG_T
Cuts at: 3425 3425
Size: 3992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2663 2847 2663
Size: 184 3808
MscI TGG'CCA
Cuts at: 1753 1753
Size: 3992
MslI CAynn'nnrTG
Cuts at: 1981 2108 1981
Size: 127 3865
MspA1I CmG'CkG
Cuts at: 1103 1417 1777 2790 3035 1103
Size: 314 360 1013 245 2060
Fragments arranged by size:
2060 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2173 2173
Size: 3992
NarI GG'CG_CC
Cuts at: 1423 1671 1423
Size: 248 3744
NcoI C'CATG_G
Cuts at: 107 107
Size: 3992
NgoMIV G'CCGG_C
Cuts at: 2171 2171
Size: 3992
NsiI A_TGCA'T
Cuts at: 375 375
Size: 3992
NspI r_CATG'y
Cuts at: 1045 1545 2452 1045
Size: 500 907 2585
Fragments arranged by size:
2585 907 500
PciI A'CATG_T
Cuts at: 2448 2448
Size: 3992
PfoI T'CCnGG_A
Cuts at: 237 237
Size: 3992
PmeI GTTT'AAAC
Cuts at: 991 991
Size: 3992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 3960
PspOMI G'GGCC_C
Cuts at: 801 801
Size: 3992
PstI C_TGCA'G
Cuts at: 1039 1724 1039
Size: 685 3307
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3992
PvuII CAG'CTG
Cuts at: 1417 1777 1417
Size: 360 3632
RsrII CG'GwC_CG
Cuts at: 2187 2187
Size: 3992
SacI G_AGCT'C
Cuts at: 1006 1006
Size: 3992
SalI G'TCGA_C
Cuts at: 1029 1029
Size: 3992
SapI GCTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
SbfI CC_TGCA'GG
Cuts at: 1039 1039
Size: 3992
ScaI AGT'ACT
Cuts at: 3509 3509
Size: 3992
SfcI C'TryA_G
Cuts at: 33 1035 1720 2713 2904 33
Size: 1002 685 993 191 1121
Fragments arranged by size:
1121 1002 993 685 191
SfoI GGC'GCC
Cuts at: 1424 1672 1424
Size: 248 3744
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
SmaI CCC'GGG
Cuts at: 1014 1014
Size: 3992
SmlI C'TyrA_G
Cuts at: 848 1147 2062 2554 2816 848
Size: 299 915 492 262 2024
Fragments arranged by size:
2024 915 492 299 262
SnaBI TAC'GTA
Cuts at: 739 739
Size: 3992
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3992
SphI G_CATG'C
Cuts at: 1045 1545 1045
Size: 500 3492
SspI AAT'ATT
Cuts at: 513 513
Size: 3992
StyI C'CwwG_G
Cuts at: 107 1126 1480 107
Size: 1019 354 2619
Fragments arranged by size:
2619 1019 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1575 1708 2167 2376 1575
Size: 133 459 209 3191
Fragments arranged by size:
3191 459 209 133
TatI w'GTAC_w
Cuts at: 483 817 3507 483
Size: 334 2690 968
Fragments arranged by size:
2690 968 334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 3960
Tth111I GACn'n_nGTC
Cuts at: 1789 1789
Size: 3992
XbaI T'CTAG_A
Cuts at: 1023 1023
Size: 3992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1487 1487
Size: 3992
XmaI C'CCGG_G
Cuts at: 1012 1012
Size: 3992
ZraI GAC'GTC
Cuts at: 716 716
Size: 3992
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI StuI SwaI
XhoI XmnI