Restriction map for
pF1KE0701
(Circular) (Six-base) MAPSORT of: pF1KE0701.seq Check: 8966 from: 1 to: 3974
pF1KE0701 3974 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 339 339
Size: 3974
AccI GT'mk_AC
Cuts at: 464 1012 464
Size: 548 3426
Acc65I G'GTAC_C
Cuts at: 990 990
Size: 3974
AclI AA'CG_TT
Cuts at: 339 3814 339
Size: 3475 499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 1799 2231 2978 3246 932
Size: 867 432 747 268 1660
Fragments arranged by size:
1660 867 747 432 268
AflIII A'CryG_T
Cuts at: 1326 2430 3407 1326
Size: 1104 977 1893
Fragments arranged by size:
1893 1104 977
AgeI A'CCGG_T
Cuts at: 2362 2362
Size: 3974
AhdI GACnn_n'nnGTC
Cuts at: 942 942
Size: 3974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 148 180 148
Size: 32 3942
AlwNI CAG_nnn'CTG
Cuts at: 933 1279 2846 933
Size: 346 1567 2061
Fragments arranged by size:
2061 1567 346
ApaLI G'TGCA_C
Cuts at: 2744 2744
Size: 3974
ApoI r'AATT_y
Cuts at: 259 448 535 978 3327 3583 259
Size: 189 87 443 2349 256 650
Fragments arranged by size:
2349 650 443 256 189 87
AseI AT'TA_AT
Cuts at: 20 302 20
Size: 282 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
AvaI C'yCGr_G
Cuts at: 994 994
Size: 3974
BamHI G'GATC_C
Cuts at: 233 999 233
Size: 766 3208
BanI G'GyrC_C
Cuts at: 990 1404 1652 1687 990
Size: 414 248 35 3277
Fragments arranged by size:
3277 414 248 35
BanII G_rGCy'C
Cuts at: 567 988 2018 567
Size: 421 1030 2523
Fragments arranged by size:
2523 1030 421
BbeI G_GCGC'C
Cuts at: 1408 1656 1408
Size: 248 3726
BbsI GAAGACnn'nnnn_
Cuts at: 839 938 839
Size: 99 3875
BciVI GTATCCnnnnn_n'
Cuts at: 1872 2633 3974 1872
Size: 761 1341 1872
Fragments arranged by size:
1872 1341 761
BclI T'GATC_A
Cuts at: 1494 1494
Size: 3974
BfrBI ATG'CAT
Cuts at: 826 826
Size: 3974
BglI GCCn_nnn'nGGC
Cuts at: 112 612 112
Size: 500 3474
BglII A'GATC_T
Cuts at: 1489 3526 1489
Size: 2037 1937
BlpI GC'TnA_GC
Cuts at: 616 1086 616
Size: 470 3504
Bme1580I G_kGCm'C
Cuts at: 1599 1692 2748 1599
Size: 93 1056 2825
Fragments arranged by size:
2825 1056 93
BmgBI CAC'GTC
Cuts at: 426 426
Size: 3974
BmrI ACTGGGnnnn_n'
Cuts at: 239 604 765 1186 1267 1602 3746 239
Size: 365 161 421 81 335 2144 467
Fragments arranged by size:
2144 467 421 365 335 161 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 3974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 150
Size: 3974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 2029 2521 2819 1150
Size: 879 492 298 2305
Fragments arranged by size:
2305 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 184 3678 184
Size: 3494 480
BsaAI yAC'GTr
Cuts at: 1327 1327
Size: 3974
BsaBI GATnn'nnATC
Cuts at: 6 1488 3525 6
Size: 1482 2037 455
Fragments arranged by size:
2037 1482 455
BsaHI Gr'CG_yC
Cuts at: 1405 1653 2355 3851 1405
Size: 248 702 1496 1528
Fragments arranged by size:
1528 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1163 1684 2362 2636 2783 3194 1163
Size: 521 678 274 147 411 1943
Fragments arranged by size:
1943 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 148 178 473 503 148
Size: 30 295 30 3619
Fragments arranged by size:
3619 295 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 907 907
Size: 3974
BseYI C'CCAG_C
Cuts at: 237 1399 2157 2734 3230 237
Size: 1162 758 577 496 981
Fragments arranged by size:
1162 981 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1516 1562 2770 104
Size: 1412 46 1208 1308
Fragments arranged by size:
1412 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 793 988 1766 1956 2748 793
Size: 195 778 190 792 2019
Fragments arranged by size:
2019 792 778 195 190
Bsp1286I G_dGCh'C
Cuts at: 567 793 988 1599 1692 1766 1956 2018 2748
Size: 226 195 611 93 74 190 62 730
Cuts at: 2748 567
Size: 1793
Fragments arranged by size:
1793 730 611 226 195 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3194 3194
Size: 3974
BspHI T'CATG_A
Cuts at: 3150 3150
Size: 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 339 1024 1238 1540 1921 339
Size: 685 214 302 381 2392
Fragments arranged by size:
2392 685 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2266 2320 3804 3969 50
Size: 2216 54 1484 165 55
Fragments arranged by size:
2216 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1886 1886
Size: 3974
BsrFI r'CCGG_y
Cuts at: 1972 2153 2362 1972
Size: 181 209 3584
Fragments arranged by size:
3584 209 181
BsrGI T'GTAC_A
Cuts at: 671 671
Size: 3974
BssSI C'ACGA_G
Cuts at: 2245 2603 2245
Size: 358 3616
BstAPI GCAn_nnn'nTGC
Cuts at: 1351 1351
Size: 3974
BstBI TT'CG_AA
Cuts at: 2335 2335
Size: 3974
BstYI r'GATC_y
Cuts at: 1 233 953 999 1466 1489 1824 2070 3082
Size: 232 720 46 467 23 335 246 1012
Cuts at: 3082 3168 3180 3526 1
Size: 86 12 346 449
Fragments arranged by size:
1012 720 467 449 346 335 246 232
86 46 23 12
BtgI C'CryG_G
Cuts at: 107 182 107
Size: 75 3899
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1924 2105 2268 1924
Size: 181 163 3630
Fragments arranged by size:
3630 181 163
BtsI GCAGTG_nn'
Cuts at: 593 1217 593
Size: 624 3350
DraI TTT'AAA
Cuts at: 345 973 345
Size: 628 3346
DrdI GACnn_nn'nnGTC
Cuts at: 510 1680 2538 510
Size: 1170 858 1946
Fragments arranged by size:
1946 1170 858
EaeI y'GGCC_r
Cuts at: 113 272 1559 1733 2124 2151 3496 113
Size: 159 1287 174 391 27 1345 591
Fragments arranged by size:
1345 1287 591 391 174 159 27
EagI C'GGCC_G
Cuts at: 1559 1559
Size: 3974
EarI CTCTTCn'nnn_
Cuts at: 1997 2207 1997
Size: 210 3764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2182 2492 2638 3784 2182
Size: 310 146 1146 2372
Fragments arranged by size:
2372 1146 310 146
EcoICRI GAG'CTC
Cuts at: 986 986
Size: 3974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 932 1799 2231 2978 3246 150
Size: 782 867 432 747 268 878
Fragments arranged by size:
878 867 782 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 411 1113 411
Size: 702 3272
EcoRI G'AATT_C
Cuts at: 448 978 448
Size: 530 3444
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1993 1993
Size: 3974
FspI TGC'GCA
Cuts at: 1755 1755
Size: 3974
HaeII r_GCGC'y
Cuts at: 1408 1656 2678 3657 1408
Size: 248 1022 979 1725
Fragments arranged by size:
1725 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 903 928 935 960 1816 1846 1848 1878 903
Size: 25 7 25 856 30 2 30 2999
Fragments arranged by size:
2999 856 30 30 25 25 7 2
HincII GTy'rAC
Cuts at: 1013 1013
Size: 3974
KasI G'GCGC_C
Cuts at: 1404 1652 1404
Size: 248 3726
KpnI G_GTAC'C
Cuts at: 994 994
Size: 3974
MluI A'CGCG_T
Cuts at: 3407 3407
Size: 3974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 795 2645 2829 795
Size: 1850 184 1940
Fragments arranged by size:
1940 1850 184
MscI TGG'CCA
Cuts at: 274 1735 274
Size: 1461 2513
MslI CAynn'nnrTG
Cuts at: 1963 2090 1963
Size: 127 3847
MspA1I CmG'CkG
Cuts at: 241 280 849 1085 1399 1759 2772 3017 241
Size: 39 569 236 314 360 1013 245 1198
Fragments arranged by size:
1198 1013 569 360 314 245 236 39
NaeI GCC'GGC
Cuts at: 2155 2155
Size: 3974
NarI GG'CG_CC
Cuts at: 1405 1653 1405
Size: 248 3726
NcoI C'CATG_G
Cuts at: 107 182 107
Size: 75 3899
NdeI CA'TA_TG
Cuts at: 804 804
Size: 3974
NgoMIV G'CCGG_C
Cuts at: 2153 2153
Size: 3974
NsiI A_TGCA'T
Cuts at: 828 828
Size: 3974
NspI r_CATG'y
Cuts at: 441 1027 1527 2434 441
Size: 586 500 907 1981
Fragments arranged by size:
1981 907 586 500
PciI A'CATG_T
Cuts at: 2430 2430
Size: 3974
PflMI CCAn_nnn'nTGG
Cuts at: 122 122
Size: 3974
PfoI T'CCnGG_A
Cuts at: 318 318
Size: 3974
PmeI GTTT'AAAC
Cuts at: 973 973
Size: 3974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 148 180 3113 3145 148
Size: 32 2933 32 977
Fragments arranged by size:
2933 977 32 32
PpuMI rG'GwC_Cy
Cuts at: 411 411
Size: 3974
PshAI GACnn'nnGTC
Cuts at: 597 597
Size: 3974
PstI C_TGCA'G
Cuts at: 1021 1706 1021
Size: 685 3289
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3974
PvuII CAG'CTG
Cuts at: 241 280 849 1399 1759 241
Size: 39 569 550 360 2456
Fragments arranged by size:
2456 569 550 360 39
RsrII CG'GwC_CG
Cuts at: 2169 2169
Size: 3974
SacI G_AGCT'C
Cuts at: 988 988
Size: 3974
SalI G'TCGA_C
Cuts at: 1011 1011
Size: 3974
SapI GCTCTTCn'nnn_
Cuts at: 1997 2207 1997
Size: 210 3764
SbfI CC_TGCA'GG
Cuts at: 1021 1021
Size: 3974
ScaI AGT'ACT
Cuts at: 3491 3491
Size: 3974
SfcI C'TryA_G
Cuts at: 33 753 759 1017 1702 2695 2886 33
Size: 720 6 258 685 993 191 1121
Fragments arranged by size:
1121 993 720 685 258 191 6
SfoI GGC'GCC
Cuts at: 1406 1654 1406
Size: 248 3726
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
SmaI CCC'GGG
Cuts at: 996 996
Size: 3974
SmlI C'TyrA_G
Cuts at: 1129 2044 2536 2798 1129
Size: 915 492 262 2305
Fragments arranged by size:
2305 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3974
SphI G_CATG'C
Cuts at: 441 1027 1527 441
Size: 586 500 2888
Fragments arranged by size:
2888 586 500
StyI C'CwwG_G
Cuts at: 107 182 1108 1462 107
Size: 75 926 354 2619
Fragments arranged by size:
2619 926 354 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1557 1690 2149 2358 1557
Size: 133 459 209 3173
Fragments arranged by size:
3173 459 209 133
TatI w'GTAC_w
Cuts at: 671 3489 671
Size: 2818 1156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 473 505 3491 3523 473
Size: 32 2986 32 924
Fragments arranged by size:
2986 924 32 32
Tth111I GACn'n_nGTC
Cuts at: 1771 1771
Size: 3974
XbaI T'CTAG_A
Cuts at: 1005 1005
Size: 3974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 123 846 1469 123
Size: 723 623 2628
Fragments arranged by size:
2628 723 623
XmaI C'CCGG_G
Cuts at: 994 994
Size: 3974
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BmtI Bpu10I BsgI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI PacI PasI PmlI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI