Restriction map for
pF1KE0696
(Circular) (Six-base) MAPSORT of: pF1KE0696.seq Check: 8665 from: 1 to: 4325
pF1KE0696 4325 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1283 1363 1283
Size: 80 4245
Acc65I G'GTAC_C
Cuts at: 1341 1341
Size: 4325
AclI AA'CG_TT
Cuts at: 4165 4165
Size: 4325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 950 1010 2150 2582 3329 3597 950
Size: 60 1140 432 747 268 1678
Fragments arranged by size:
1678 1140 747 432 268 60
AflIII A'CryG_T
Cuts at: 270 1677 2781 3758 270
Size: 1407 1104 977 837
Fragments arranged by size:
1407 1104 977 837
AgeI A'CCGG_T
Cuts at: 2713 2713
Size: 4325
AlwNI CAG_nnn'CTG
Cuts at: 961 1630 3197 961
Size: 669 1567 2089
Fragments arranged by size:
2089 1567 669
ApaLI G'TGCA_C
Cuts at: 3095 3095
Size: 4325
ApoI r'AATT_y
Cuts at: 178 1329 3678 3934 178
Size: 1151 2349 256 569
Fragments arranged by size:
2349 1151 569 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4325
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
AvaI C'yCGr_G
Cuts at: 1345 1345
Size: 4325
BamHI G'GATC_C
Cuts at: 1350 1350
Size: 4325
BanI G'GyrC_C
Cuts at: 964 1341 1755 2003 2038 964
Size: 377 414 248 35 3251
Fragments arranged by size:
3251 414 377 248 35
BanII G_rGCy'C
Cuts at: 1339 2369 1339
Size: 1030 3295
BbeI G_GCGC'C
Cuts at: 968 1759 2007 968
Size: 791 248 3286
Fragments arranged by size:
3286 791 248
BbsI GAAGACnn'nnnn_
Cuts at: 995 995
Size: 4325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1132 1166 90
Size: 34 1008 34 3249
Fragments arranged by size:
3249 1008 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 198 2223 2984 4325 198
Size: 2025 761 1341 198
Fragments arranged by size:
2025 1341 761 198
BclI T'GATC_A
Cuts at: 393 1845 393
Size: 1452 2873
BglII A'GATC_T
Cuts at: 994 1840 3877 994
Size: 846 2037 1442
Fragments arranged by size:
2037 1442 846
BlpI GC'TnA_GC
Cuts at: 1437 1437
Size: 4325
Bme1580I G_kGCm'C
Cuts at: 1950 2043 3099 1950
Size: 93 1056 3176
Fragments arranged by size:
3176 1056 93
BmgBI CAC'GTC
Cuts at: 1317 1317
Size: 4325
BmrI ACTGGGnnnn_n'
Cuts at: 1537 1618 1953 4097 1537
Size: 81 335 2144 1765
Fragments arranged by size:
2144 1765 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4325
Bpu10I CC'TnA_GC
Cuts at: 325 325
Size: 4325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 1501 2380 2872 3170 653
Size: 848 879 492 298 1808
Fragments arranged by size:
1808 879 848 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1113 4029 1113
Size: 2916 1409
BsaAI yAC'GTr
Cuts at: 271 1678 271
Size: 1407 2918
BsaBI GATnn'nnATC
Cuts at: 6 1839 3876 6
Size: 1833 2037 455
Fragments arranged by size:
2037 1833 455
BsaHI Gr'CG_yC
Cuts at: 965 1756 2004 2706 4202 965
Size: 791 248 702 1496 1088
Fragments arranged by size:
1496 1088 791 702 248
BsaWI w'CCGG_w
Cuts at: 1305 1514 2035 2713 2987 3134 3545 1305
Size: 209 521 678 274 147 411 2085
Fragments arranged by size:
2085 678 521 411 274 209 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1176 1206 1176
Size: 30 4295
BseYI C'CCAG_C
Cuts at: 1750 2508 3085 3581 1750
Size: 758 577 496 2494
Fragments arranged by size:
2494 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 934
Size: 4325
BsiEI CG_ry'CG
Cuts at: 104 1867 1913 3121 104
Size: 1763 46 1208 1308
Fragments arranged by size:
1763 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1339 2117 2307 3099 1339
Size: 778 190 792 2565
Fragments arranged by size:
2565 792 778 190
BsmI GAATG_Cn'
Cuts at: 363 363
Size: 4325
BsmBI CGTCTCn'nnnn_
Cuts at: 902 902
Size: 4325
Bsp1286I G_dGCh'C
Cuts at: 1339 1950 2043 2117 2307 2369 3099 1339
Size: 611 93 74 190 62 730 2565
Fragments arranged by size:
2565 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3545 3545
Size: 4325
BspHI T'CATG_A
Cuts at: 3501 3501
Size: 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1375 1589 1891 2272 1375
Size: 214 302 381 3428
Fragments arranged by size:
3428 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2617 2671 4155 4320 50
Size: 2567 54 1484 165 55
Fragments arranged by size:
2567 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2237 2237
Size: 4325
BsrFI r'CCGG_y
Cuts at: 2323 2504 2713 2323
Size: 181 209 3935
Fragments arranged by size:
3935 209 181
BssSI C'ACGA_G
Cuts at: 2596 2954 2596
Size: 358 3967
BstAPI GCAn_nnn'nTGC
Cuts at: 1702 1702
Size: 4325
BstBI TT'CG_AA
Cuts at: 186 2686 186
Size: 2500 1825
BstYI r'GATC_y
Cuts at: 1 994 1350 1817 1840 2175 2421 3433 3519
Size: 993 356 467 23 335 246 1012 86
Cuts at: 3519 3531 3877 1
Size: 12 346 449
Fragments arranged by size:
1012 993 467 449 356 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1259 1259
Size: 4325
BtgI C'CryG_G
Cuts at: 498 1121 498
Size: 623 3702
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 326 2275 2456 2619 326
Size: 1949 181 163 2032
Fragments arranged by size:
2032 1949 181 163
BtsI GCAGTG_nn'
Cuts at: 722 1568 722
Size: 846 3479
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4325
DrdI GACnn_nn'nnGTC
Cuts at: 2031 2889 2031
Size: 858 3467
EaeI y'GGCC_r
Cuts at: 1910 2084 2475 2502 3847 1910
Size: 174 391 27 1345 2388
Fragments arranged by size:
2388 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1910 1910
Size: 4325
EarI CTCTTCn'nnn_
Cuts at: 727 806 926 2348 2558 727
Size: 79 120 1422 210 2494
Fragments arranged by size:
2494 1422 210 120 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 352 2533 2843 2989 4135 352
Size: 2181 310 146 1146 542
Fragments arranged by size:
2181 1146 542 310 146
EcoICRI GAG'CTC
Cuts at: 1337 1337
Size: 4325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 950 1010 2150 2582 3329 3597 950
Size: 60 1140 432 747 268 1678
Fragments arranged by size:
1678 1140 747 432 268 60
EcoO109I rG'GnC_Cy
Cuts at: 402 985 1464 402
Size: 583 479 3263
Fragments arranged by size:
3263 583 479
EcoRI G'AATT_C
Cuts at: 1329 1329
Size: 4325
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 4325
FspI TGC'GCA
Cuts at: 2106 2106
Size: 4325
HaeII r_GCGC'y
Cuts at: 968 1759 2007 3029 4008 968
Size: 791 248 1022 979 1285
Fragments arranged by size:
1285 1022 979 791 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1176 1208 2167 2197 2199 2229 1176
Size: 32 959 30 2 30 3272
Fragments arranged by size:
3272 959 32 30 30 2
HincII GTy'rAC
Cuts at: 1364 1364
Size: 4325
HindIII A'AGCT_T
Cuts at: 286 286
Size: 4325
KasI G'GCGC_C
Cuts at: 964 1755 2003 964
Size: 791 248 3286
Fragments arranged by size:
3286 791 248
KpnI G_GTAC'C
Cuts at: 1345 1345
Size: 4325
MfeI C'AATT_G
Cuts at: 748 748
Size: 4325
MluI A'CGCG_T
Cuts at: 3758 3758
Size: 4325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 255 925 2996 3180 255
Size: 670 2071 184 1400
Fragments arranged by size:
2071 1400 670 184
MscI TGG'CCA
Cuts at: 2086 2086
Size: 4325
MslI CAynn'nnrTG
Cuts at: 862 2314 2441 862
Size: 1452 127 2746
Fragments arranged by size:
2746 1452 127
MspA1I CmG'CkG
Cuts at: 981 1436 1750 2110 3123 3368 981
Size: 455 314 360 1013 245 1938
Fragments arranged by size:
1938 1013 455 360 314 245
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 4325
NarI GG'CG_CC
Cuts at: 965 1756 2004 965
Size: 791 248 3286
Fragments arranged by size:
3286 791 248
NcoI C'CATG_G
Cuts at: 498 1121 498
Size: 623 3702
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 4325
NspI r_CATG'y
Cuts at: 1378 1878 2785 1378
Size: 500 907 2918
Fragments arranged by size:
2918 907 500
PciI A'CATG_T
Cuts at: 2781 2781
Size: 4325
PfoI T'CCnGG_A
Cuts at: 836 836
Size: 4325
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4325
PmlI CAC'GTG
Cuts at: 271 271
Size: 4325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 4293
PpuMI rG'GwC_Cy
Cuts at: 402 985 402
Size: 583 3742
PstI C_TGCA'G
Cuts at: 1372 2057 1372
Size: 685 3640
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4325
PvuII CAG'CTG
Cuts at: 1750 2110 1750
Size: 360 3965
RsrII CG'GwC_CG
Cuts at: 2520 2520
Size: 4325
SacI G_AGCT'C
Cuts at: 1339 1339
Size: 4325
SalI G'TCGA_C
Cuts at: 1362 1362
Size: 4325
SanDI GG'GwC_CC
Cuts at: 985 985
Size: 4325
SapI GCTCTTCn'nnn_
Cuts at: 2348 2558 2348
Size: 210 4115
SbfI CC_TGCA'GG
Cuts at: 1372 1372
Size: 4325
ScaI AGT'ACT
Cuts at: 376 3842 376
Size: 3466 859
SfcI C'TryA_G
Cuts at: 33 1368 2053 3046 3237 33
Size: 1335 685 993 191 1121
Fragments arranged by size:
1335 1121 993 685 191
SfoI GGC'GCC
Cuts at: 966 1757 2005 966
Size: 791 248 3286
Fragments arranged by size:
3286 791 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
SmaI CCC'GGG
Cuts at: 1347 1347
Size: 4325
SmlI C'TyrA_G
Cuts at: 668 1480 2395 2887 3149 668
Size: 812 915 492 262 1844
Fragments arranged by size:
1844 915 812 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4325
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3825
StyI C'CwwG_G
Cuts at: 498 1121 1154 1459 1813 498
Size: 623 33 305 354 3010
Fragments arranged by size:
3010 623 354 305 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1908 2041 2500 2709 1908
Size: 133 459 209 3524
Fragments arranged by size:
3524 459 209 133
TatI w'GTAC_w
Cuts at: 374 3840 374
Size: 3466 859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4293
Tth111I GACn'n_nGTC
Cuts at: 2122 2122
Size: 4325
XbaI T'CTAG_A
Cuts at: 147 1356 147
Size: 1209 3116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 505 1820 114
Size: 391 1315 2619
Fragments arranged by size:
2619 1315 391
XmaI C'CCGG_G
Cuts at: 1345 1345
Size: 4325
XmnI GAAnn'nnTTC
Cuts at: 376 800 376
Size: 424 3901
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI
BpmI BseRI BsiWI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI