Restriction map for
pF1KE0694
(Circular) (Six-base) MAPSORT of: pF1KE0694.seq Check: 5258 from: 1 to: 4322
pF1KE0694 4322 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 871 1360 871
Size: 489 3833
Acc65I G'GTAC_C
Cuts at: 1338 1338
Size: 4322
AclI AA'CG_TT
Cuts at: 4162 4162
Size: 4322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 989 2147 2579 3326 3594 494
Size: 495 1158 432 747 268 1222
Fragments arranged by size:
1222 1158 747 495 432 268
AflIII A'CryG_T
Cuts at: 1674 2778 3755 1674
Size: 1104 977 2241
Fragments arranged by size:
2241 1104 977
AgeI A'CCGG_T
Cuts at: 2710 2710
Size: 4322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 542 542
Size: 4322
AlwNI CAG_nnn'CTG
Cuts at: 1627 3194 1627
Size: 1567 2755
ApaI G_GGCC'C
Cuts at: 190 190
Size: 4322
ApaLI G'TGCA_C
Cuts at: 3092 3092
Size: 4322
ApoI r'AATT_y
Cuts at: 155 366 977 1103 1326 3675 3931 155
Size: 211 611 126 223 2349 256 546
Fragments arranged by size:
2349 611 546 256 223 211 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 4322
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
AvaI C'yCGr_G
Cuts at: 1342 1342
Size: 4322
BamHI G'GATC_C
Cuts at: 1347 1347
Size: 4322
BanI G'GyrC_C
Cuts at: 355 1338 1752 2000 2035 355
Size: 983 414 248 35 2642
Fragments arranged by size:
2642 983 414 248 35
BanII G_rGCy'C
Cuts at: 190 1336 2366 190
Size: 1146 1030 2146
Fragments arranged by size:
2146 1146 1030
BbeI G_GCGC'C
Cuts at: 1756 2004 1756
Size: 248 4074
BbsI GAAGACnn'nnnn_
Cuts at: 737 737
Size: 4322
BbvCI CC'TCA_GC
Cuts at: 776 795 776
Size: 19 4303
BciVI GTATCCnnnnn_n'
Cuts at: 2220 2981 4322 2220
Size: 761 1341 2220
Fragments arranged by size:
2220 1341 761
BclI T'GATC_A
Cuts at: 1842 1842
Size: 4322
BfrBI ATG'CAT
Cuts at: 523 523
Size: 4322
BglI GCCn_nnn'nGGC
Cuts at: 1064 1064
Size: 4322
BglII A'GATC_T
Cuts at: 1311 1837 3874 1311
Size: 526 2037 1759
Fragments arranged by size:
2037 1759 526
BlpI GC'TnA_GC
Cuts at: 1434 1434
Size: 4322
Bme1580I G_kGCm'C
Cuts at: 190 263 1947 2040 3096 190
Size: 73 1684 93 1056 1416
Fragments arranged by size:
1684 1416 1056 93 73
BmrI ACTGGGnnnn_n'
Cuts at: 446 915 1534 1615 1950 4094 446
Size: 469 619 81 335 2144 674
Fragments arranged by size:
2144 674 619 469 335 81
BmtI G_CTAG'C
Cuts at: 1058 1058
Size: 4322
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 4322
Bpu10I CC'TnA_GC
Cuts at: 776 795 776
Size: 19 4303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1498 2377 2869 3167 1498
Size: 879 492 298 2653
Fragments arranged by size:
2653 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 139 4026 139
Size: 3887 435
BsaAI yAC'GTr
Cuts at: 1675 1675
Size: 4322
BsaBI GATnn'nnATC
Cuts at: 6 1836 3873 6
Size: 1830 2037 455
Fragments arranged by size:
2037 1830 455
BsaHI Gr'CG_yC
Cuts at: 112 1753 2001 2703 4199 112
Size: 1641 248 702 1496 235
Fragments arranged by size:
1641 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 1511 2032 2710 2984 3131 3542 1511
Size: 521 678 274 147 411 2291
Fragments arranged by size:
2291 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1187 1217 1187
Size: 30 4292
BseYI C'CCAG_C
Cuts at: 116 414 1747 2505 3082 3578 116
Size: 298 1333 758 577 496 860
Fragments arranged by size:
1333 860 758 577 496 298
BsiEI CG_ry'CG
Cuts at: 104 1864 1910 3118 104
Size: 1760 46 1208 1308
Fragments arranged by size:
1760 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1336 2114 2304 3096 1336
Size: 778 190 792 2562
Fragments arranged by size:
2562 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 190 263 1336 1947 2040 2114 2304 2366 3096
Size: 73 1073 611 93 74 190 62 730
Cuts at: 3096 190
Size: 1416
Fragments arranged by size:
1416 1073 730 611 190 93 74 73
62
BspEI T'CCGG_A
Cuts at: 3542 3542
Size: 4322
BspHI T'CATG_A
Cuts at: 3498 3498
Size: 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 333 1095 1372 1586 1888 2269 333
Size: 762 277 214 302 381 2386
Fragments arranged by size:
2386 762 381 302 277 214
BsrBI CCG'CTC
Cuts at: 50 2614 2668 4152 4317 50
Size: 2564 54 1484 165 55
Fragments arranged by size:
2564 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2234 2234
Size: 4322
BsrFI r'CCGG_y
Cuts at: 2320 2501 2710 2320
Size: 181 209 3932
Fragments arranged by size:
3932 209 181
BssSI C'ACGA_G
Cuts at: 2593 2951 2593
Size: 358 3964
BstAPI GCAn_nnn'nTGC
Cuts at: 1699 1699
Size: 4322
BstBI TT'CG_AA
Cuts at: 2683 2683
Size: 4322
BstYI r'GATC_y
Cuts at: 1 1311 1347 1814 1837 2172 2418 3430 3516
Size: 1310 36 467 23 335 246 1012 86
Cuts at: 3516 3528 3874 1
Size: 12 346 449
Fragments arranged by size:
1310 1012 467 449 346 335 246 86
36 23 12
BtgI C'CryG_G
Cuts at: 107 1199 107
Size: 1092 3230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2272 2453 2616 2272
Size: 181 163 3978
Fragments arranged by size:
3978 181 163
BtsI GCAGTG_nn'
Cuts at: 312 1565 312
Size: 1253 3069
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1020 1055 1020
Size: 35 4287
DraI TTT'AAA
Cuts at: 1321 1321
Size: 4322
DrdI GACnn_nn'nnGTC
Cuts at: 2028 2886 2028
Size: 858 3464
EaeI y'GGCC_r
Cuts at: 808 1065 1907 2081 2472 2499 3844 808
Size: 257 842 174 391 27 1345 1286
Fragments arranged by size:
1345 1286 842 391 257 174 27
EagI C'GGCC_G
Cuts at: 1907 1907
Size: 4322
EarI CTCTTCn'nnn_
Cuts at: 708 2345 2555 708
Size: 1637 210 2475
Fragments arranged by size:
2475 1637 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2530 2840 2986 4132 2530
Size: 310 146 1146 2720
Fragments arranged by size:
2720 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1334 1334
Size: 4322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 989 2147 2579 3326 3594 494
Size: 495 1158 432 747 268 1222
Fragments arranged by size:
1222 1158 747 495 432 268
EcoO109I rG'GnC_Cy
Cuts at: 187 214 1461 187
Size: 27 1247 3048
Fragments arranged by size:
3048 1247 27
EcoRI G'AATT_C
Cuts at: 155 1326 155
Size: 1171 3151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 4322
FspI TGC'GCA
Cuts at: 2103 2103
Size: 4322
HaeII r_GCGC'y
Cuts at: 1756 2004 3026 4005 1756
Size: 248 1022 979 2073
Fragments arranged by size:
2073 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2164 2194 2196 2226 2164
Size: 30 2 30 4260
Fragments arranged by size:
4260 30 30 2
HincII GTy'rAC
Cuts at: 1361 1361
Size: 4322
KasI G'GCGC_C
Cuts at: 1752 2000 1752
Size: 248 4074
KpnI G_GTAC'C
Cuts at: 1342 1342
Size: 4322
MluI A'CGCG_T
Cuts at: 3755 3755
Size: 4322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2993 3177 2993
Size: 184 4138
MscI TGG'CCA
Cuts at: 810 1067 2083 810
Size: 257 1016 3049
Fragments arranged by size:
3049 1016 257
MslI CAynn'nnrTG
Cuts at: 881 2311 2438 881
Size: 1430 127 2765
Fragments arranged by size:
2765 1430 127
MspA1I CmG'CkG
Cuts at: 565 696 1433 1747 2107 3120 3365 565
Size: 131 737 314 360 1013 245 1522
Fragments arranged by size:
1522 1013 737 360 314 245 131
NaeI GCC'GGC
Cuts at: 2503 2503
Size: 4322
NarI GG'CG_CC
Cuts at: 1753 2001 1753
Size: 248 4074
NcoI C'CATG_G
Cuts at: 107 107
Size: 4322
NgoMIV G'CCGG_C
Cuts at: 2501 2501
Size: 4322
NheI G'CTAG_C
Cuts at: 1054 1054
Size: 4322
NsiI A_TGCA'T
Cuts at: 525 525
Size: 4322
NspI r_CATG'y
Cuts at: 523 767 1375 1875 2782 523
Size: 244 608 500 907 2063
Fragments arranged by size:
2063 907 608 500 244
PasI CC'CwG_GG
Cuts at: 140 140
Size: 4322
PciI A'CATG_T
Cuts at: 2778 2778
Size: 4322
PflMI CCAn_nnn'nTGG
Cuts at: 140 140
Size: 4322
PfoI T'CCnGG_A
Cuts at: 159 159
Size: 4322
PmeI GTTT'AAAC
Cuts at: 1321 1321
Size: 4322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 3461
Size: 32 4290
PpuMI rG'GwC_Cy
Cuts at: 214 214
Size: 4322
PspOMI G'GGCC_C
Cuts at: 186 186
Size: 4322
PstI C_TGCA'G
Cuts at: 1369 2054 1369
Size: 685 3637
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4322
PvuII CAG'CTG
Cuts at: 565 1747 2107 565
Size: 1182 360 2780
Fragments arranged by size:
2780 1182 360
RsrII CG'GwC_CG
Cuts at: 169 2517 169
Size: 2348 1974
SacI G_AGCT'C
Cuts at: 1336 1336
Size: 4322
SalI G'TCGA_C
Cuts at: 1359 1359
Size: 4322
SapI GCTCTTCn'nnn_
Cuts at: 2345 2555 2345
Size: 210 4112
SbfI CC_TGCA'GG
Cuts at: 1369 1369
Size: 4322
ScaI AGT'ACT
Cuts at: 3839 3839
Size: 4322
SfcI C'TryA_G
Cuts at: 33 384 691 1365 2050 3043 3234 33
Size: 351 307 674 685 993 191 1121
Fragments arranged by size:
1121 993 685 674 351 307 191
SfoI GGC'GCC
Cuts at: 1754 2002 1754
Size: 248 4074
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
SmaI CCC'GGG
Cuts at: 1344 1344
Size: 4322
SmlI C'TyrA_G
Cuts at: 1477 2392 2884 3146 1477
Size: 915 492 262 2653
Fragments arranged by size:
2653 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4322
SphI G_CATG'C
Cuts at: 523 767 1375 1875 523
Size: 244 608 500 2970
Fragments arranged by size:
2970 608 500 244
SspI AAT'ATT
Cuts at: 815 815
Size: 4322
StyI C'CwwG_G
Cuts at: 107 277 768 837 1456 1810 107
Size: 170 491 69 619 354 2619
Fragments arranged by size:
2619 619 491 354 170 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 465 797 1905 2038 2497 2706 465
Size: 332 1108 133 459 209 2081
Fragments arranged by size:
2081 1108 459 332 209 133
TatI w'GTAC_w
Cuts at: 3837 3837
Size: 4322
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 4290
Tth111I GACn'n_nGTC
Cuts at: 2119 2119
Size: 4322
XbaI T'CTAG_A
Cuts at: 1353 1353
Size: 4322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1817 1817
Size: 4322
XmaI C'CCGG_G
Cuts at: 1342 1342
Size: 4322
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BmgBI BpmI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI PacI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI