Restriction map for
pF1KE0692
(Circular) (Six-base) MAPSORT of: pF1KE0692.seq Check: 7005 from: 1 to: 4319
pF1KE0692 4319 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 388 388
Size: 4319
AccI GT'mk_AC
Cuts at: 704 1357 704
Size: 653 3666
Acc65I G'GTAC_C
Cuts at: 1335 1335
Size: 4319
AclI AA'CG_TT
Cuts at: 4159 4159
Size: 4319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 782 1253 2144 2576 3323 3591 782
Size: 471 891 432 747 268 1510
Fragments arranged by size:
1510 891 747 471 432 268
AflIII A'CryG_T
Cuts at: 1671 2775 3752 1671
Size: 1104 977 2238
Fragments arranged by size:
2238 1104 977
AgeI A'CCGG_T
Cuts at: 2707 2707
Size: 4319
AlwNI CAG_nnn'CTG
Cuts at: 1624 3191 1624
Size: 1567 2752
ApaLI G'TGCA_C
Cuts at: 555 3089 555
Size: 2534 1785
ApoI r'AATT_y
Cuts at: 991 1085 1323 3672 3928 991
Size: 94 238 2349 256 1382
Fragments arranged by size:
2349 1382 256 238 94
AseI AT'TA_AT
Cuts at: 20 20
Size: 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
AvaI C'yCGr_G
Cuts at: 1339 1339
Size: 4319
BamHI G'GATC_C
Cuts at: 1344 1344
Size: 4319
BanI G'GyrC_C
Cuts at: 169 570 724 1335 1749 1997 2032 169
Size: 401 154 611 414 248 35 2456
Fragments arranged by size:
2456 611 414 401 248 154 35
BanII G_rGCy'C
Cuts at: 1333 2363 1333
Size: 1030 3289
BbeI G_GCGC'C
Cuts at: 173 1753 2001 173
Size: 1580 248 2491
Fragments arranged by size:
2491 1580 248
BbsI GAAGACnn'nnnn_
Cuts at: 314 1073 314
Size: 759 3560
BciVI GTATCCnnnnn_n'
Cuts at: 2217 2978 4319 2217
Size: 761 1341 2217
Fragments arranged by size:
2217 1341 761
BclI T'GATC_A
Cuts at: 1839 1839
Size: 4319
BglII A'GATC_T
Cuts at: 801 1217 1834 3871 801
Size: 416 617 2037 1249
Fragments arranged by size:
2037 1249 617 416
BlpI GC'TnA_GC
Cuts at: 506 1431 506
Size: 925 3394
Bme1580I G_kGCm'C
Cuts at: 559 1944 2037 3093 559
Size: 1385 93 1056 1785
Fragments arranged by size:
1785 1385 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1531 1612 1947 4091 1531
Size: 81 335 2144 1759
Fragments arranged by size:
2144 1759 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 4319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 339 356 205
Size: 134 17 4168
Fragments arranged by size:
4168 134 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 2374 2866 3164 1495
Size: 879 492 298 2650
Fragments arranged by size:
2650 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 703 4023 703
Size: 3320 999
BsaAI yAC'GTr
Cuts at: 1672 1672
Size: 4319
BsaBI GATnn'nnATC
Cuts at: 6 541 1833 3870 6
Size: 535 1292 2037 455
Fragments arranged by size:
2037 1292 535 455
BsaHI Gr'CG_yC
Cuts at: 170 385 1750 1998 2700 4196 170
Size: 215 1365 248 702 1496 293
Fragments arranged by size:
1496 1365 702 293 248 215
BsaWI w'CCGG_w
Cuts at: 1508 2029 2707 2981 3128 3539 1508
Size: 521 678 274 147 411 2288
Fragments arranged by size:
2288 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 258 288 607 637 258
Size: 30 319 30 3940
Fragments arranged by size:
3940 319 30 30
BseYI C'CCAG_C
Cuts at: 349 575 1744 2502 3079 3575 349
Size: 226 1169 758 577 496 1093
Fragments arranged by size:
1169 1093 758 577 496 226
BsiEI CG_ry'CG
Cuts at: 104 1861 1907 3115 104
Size: 1757 46 1208 1308
Fragments arranged by size:
1757 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 559 1333 2111 2301 3093 559
Size: 774 778 190 792 1785
Fragments arranged by size:
1785 792 778 774 190
Bsp1286I G_dGCh'C
Cuts at: 559 1333 1944 2037 2111 2301 2363 3093 559
Size: 774 611 93 74 190 62 730 1785
Fragments arranged by size:
1785 774 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3539 3539
Size: 4319
BspHI T'CATG_A
Cuts at: 438 639 3495 438
Size: 201 2856 1262
Fragments arranged by size:
2856 1262 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1369 1583 1885 2266 1369
Size: 214 302 381 3422
Fragments arranged by size:
3422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2611 2665 4149 4314 50
Size: 2561 54 1484 165 55
Fragments arranged by size:
2561 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 674 2231 674
Size: 1557 2762
BsrFI r'CCGG_y
Cuts at: 2317 2498 2707 2317
Size: 181 209 3929
Fragments arranged by size:
3929 209 181
BsrGI T'GTAC_A
Cuts at: 986 986
Size: 4319
BssSI C'ACGA_G
Cuts at: 2590 2948 2590
Size: 358 3961
BstAPI GCAn_nnn'nTGC
Cuts at: 516 1696 516
Size: 1180 3139
BstBI TT'CG_AA
Cuts at: 2680 2680
Size: 4319
BstYI r'GATC_y
Cuts at: 1 801 912 940 1217 1344 1811 1834 2169
Size: 800 111 28 277 127 467 23 335
Cuts at: 2169 2415 3427 3513 3525 3871 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 800 467 449 346 335 277 246
127 111 86 28 23 12
Bsu36I CC'TnA_GG
Cuts at: 818 853 818
Size: 35 4284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2269 2450 2613 2269
Size: 181 163 3975
Fragments arranged by size:
3975 181 163
BtsI GCAGTG_nn'
Cuts at: 771 1562 771
Size: 791 3528
DraI TTT'AAA
Cuts at: 1318 1318
Size: 4319
DraIII CAC_nnn'GTG
Cuts at: 333 333
Size: 4319
DrdI GACnn_nn'nnGTC
Cuts at: 2025 2883 2025
Size: 858 3461
EaeI y'GGCC_r
Cuts at: 1904 2078 2469 2496 3841 1904
Size: 174 391 27 1345 2382
Fragments arranged by size:
2382 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1904 1904
Size: 4319
EarI CTCTTCn'nnn_
Cuts at: 2342 2552 2342
Size: 210 4109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2527 2837 2983 4129 2527
Size: 310 146 1146 2717
Fragments arranged by size:
2717 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1331 1331
Size: 4319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 339 356 782 1253 2144 2576 3323 3591
Size: 134 17 426 471 891 432 747 268
Cuts at: 3591 205
Size: 933
Fragments arranged by size:
933 891 747 471 432 426 268 134
17
EcoNI CCTnn'n_nnAGG
Cuts at: 371 371
Size: 4319
EcoO109I rG'GnC_Cy
Cuts at: 1458 1458
Size: 4319
EcoRI G'AATT_C
Cuts at: 1323 1323
Size: 4319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 4319
FspI TGC'GCA
Cuts at: 2100 2100
Size: 4319
HaeII r_GCGC'y
Cuts at: 173 1753 2001 3023 4002 173
Size: 1580 248 1022 979 490
Fragments arranged by size:
1580 1022 979 490 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2161 2191 2193 2223 2161
Size: 30 2 30 4257
Fragments arranged by size:
4257 30 30 2
HincII GTy'rAC
Cuts at: 1358 1358
Size: 4319
KasI G'GCGC_C
Cuts at: 169 1749 1997 169
Size: 1580 248 2491
Fragments arranged by size:
2491 1580 248
KpnI G_GTAC'C
Cuts at: 1339 1339
Size: 4319
MluI A'CGCG_T
Cuts at: 3752 3752
Size: 4319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2990 3174 2990
Size: 184 4135
MscI TGG'CCA
Cuts at: 2080 2080
Size: 4319
MslI CAynn'nnrTG
Cuts at: 416 490 2308 2435 416
Size: 74 1818 127 2300
Fragments arranged by size:
2300 1818 127 74
MspA1I CmG'CkG
Cuts at: 579 1430 1744 2104 3117 3362 579
Size: 851 314 360 1013 245 1536
Fragments arranged by size:
1536 1013 851 360 314 245
NaeI GCC'GGC
Cuts at: 2500 2500
Size: 4319
NarI GG'CG_CC
Cuts at: 170 1750 1998 170
Size: 1580 248 2491
Fragments arranged by size:
2491 1580 248
NgoMIV G'CCGG_C
Cuts at: 2498 2498
Size: 4319
NspI r_CATG'y
Cuts at: 1372 1872 2779 1372
Size: 500 907 2912
Fragments arranged by size:
2912 907 500
PciI A'CATG_T
Cuts at: 2775 2775
Size: 4319
PflMI CCAn_nnn'nTGG
Cuts at: 892 892
Size: 4319
PfoI T'CCnGG_A
Cuts at: 372 372
Size: 4319
PmeI GTTT'AAAC
Cuts at: 1318 1318
Size: 4319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 4287
PstI C_TGCA'G
Cuts at: 1366 2051 1366
Size: 685 3634
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4319
PvuII CAG'CTG
Cuts at: 1744 2104 1744
Size: 360 3959
RsrII CG'GwC_CG
Cuts at: 2514 2514
Size: 4319
SacI G_AGCT'C
Cuts at: 1333 1333
Size: 4319
SalI G'TCGA_C
Cuts at: 1356 1356
Size: 4319
SapI GCTCTTCn'nnn_
Cuts at: 2342 2552 2342
Size: 210 4109
SbfI CC_TGCA'GG
Cuts at: 1366 1366
Size: 4319
ScaI AGT'ACT
Cuts at: 3836 3836
Size: 4319
SfcI C'TryA_G
Cuts at: 33 618 1362 2047 3040 3231 33
Size: 585 744 685 993 191 1121
Fragments arranged by size:
1121 993 744 685 585 191
SfoI GGC'GCC
Cuts at: 171 1751 1999 171
Size: 1580 248 2491
Fragments arranged by size:
2491 1580 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
SmaI CCC'GGG
Cuts at: 1341 1341
Size: 4319
SmlI C'TyrA_G
Cuts at: 1474 2389 2881 3143 1474
Size: 915 492 262 2650
Fragments arranged by size:
2650 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4319
SphI G_CATG'C
Cuts at: 1372 1872 1372
Size: 500 3819
StuI AGG'CCT
Cuts at: 1283 1283
Size: 4319
StyI C'CwwG_G
Cuts at: 155 1453 1807 155
Size: 1298 354 2667
Fragments arranged by size:
2667 1298 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1166 1902 2035 2494 2703 1166
Size: 736 133 459 209 2782
Fragments arranged by size:
2782 736 459 209 133
TatI w'GTAC_w
Cuts at: 986 1299 3834 986
Size: 313 2535 1471
Fragments arranged by size:
2535 1471 313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 258 290 3836 3868 258
Size: 32 3546 32 709
Fragments arranged by size:
3546 709 32 32
Tth111I GACn'n_nGTC
Cuts at: 700 2116 700
Size: 1416 2903
XbaI T'CTAG_A
Cuts at: 1350 1350
Size: 4319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 162 1814 162
Size: 1652 2667
XmaI C'CCGG_G
Cuts at: 1339 1339
Size: 4319
ZraI GAC'GTC
Cuts at: 386 386
Size: 4319
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I BtgI ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI