Restriction map for
pF1KE0691
(Circular) (Six-base) MAPSORT of: pF1KE0691.seq Check: 9198 from: 1 to: 3956
pF1KE0691 3956 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 762 762
Size: 3956
AccI GT'mk_AC
Cuts at: 994 994
Size: 3956
Acc65I G'GTAC_C
Cuts at: 972 972
Size: 3956
AclI AA'CG_TT
Cuts at: 3796 3796
Size: 3956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1781 2213 2960 3228 1781
Size: 432 747 268 2509
Fragments arranged by size:
2509 747 432 268
AflIII A'CryG_T
Cuts at: 1308 2412 3389 1308
Size: 1104 977 1875
Fragments arranged by size:
1875 1104 977
AgeI A'CCGG_T
Cuts at: 719 2344 719
Size: 1625 2331
AlwNI CAG_nnn'CTG
Cuts at: 196 1261 2828 196
Size: 1065 1567 1324
Fragments arranged by size:
1567 1324 1065
ApaLI G'TGCA_C
Cuts at: 2726 2726
Size: 3956
ApoI r'AATT_y
Cuts at: 960 3309 3565 960
Size: 2349 256 1351
Fragments arranged by size:
2349 1351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
AvaI C'yCGr_G
Cuts at: 128 136 501 976 128
Size: 8 365 475 3108
Fragments arranged by size:
3108 475 365 8
BamHI G'GATC_C
Cuts at: 981 981
Size: 3956
BanI G'GyrC_C
Cuts at: 120 528 972 1386 1634 1669 120
Size: 408 444 414 248 35 2407
Fragments arranged by size:
2407 444 414 408 248 35
BanII G_rGCy'C
Cuts at: 608 970 2000 608
Size: 362 1030 2564
Fragments arranged by size:
2564 1030 362
BbeI G_GCGC'C
Cuts at: 124 1390 1638 124
Size: 1266 248 2442
Fragments arranged by size:
2442 1266 248
BbsI GAAGACnn'nnnn_
Cuts at: 361 361
Size: 3956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 162 196 162
Size: 34 3922
BciVI GTATCCnnnnn_n'
Cuts at: 803 1854 2615 3956 803
Size: 1051 761 1341 803
Fragments arranged by size:
1341 1051 803 761
BclI T'GATC_A
Cuts at: 1476 1476
Size: 3956
BglI GCCn_nnn'nGGC
Cuts at: 118 259 118
Size: 141 3815
BglII A'GATC_T
Cuts at: 1471 3508 1471
Size: 2037 1919
BlpI GC'TnA_GC
Cuts at: 1068 1068
Size: 3956
Bme1580I G_kGCm'C
Cuts at: 533 778 1581 1674 2730 533
Size: 245 803 93 1056 1759
Fragments arranged by size:
1759 1056 803 245 93
BmrI ACTGGGnnnn_n'
Cuts at: 1168 1249 1584 3728 1168
Size: 81 335 2144 1396
Fragments arranged by size:
2144 1396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 222 2323 222
Size: 2101 1855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 403 466 592 607 916 328
Size: 75 63 126 15 309 3368
Fragments arranged by size:
3368 309 126 75 63 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 2011 2503 2801 1132
Size: 879 492 298 2287
Fragments arranged by size:
2287 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 651 3660 651
Size: 3009 947
BsaAI yAC'GTr
Cuts at: 618 1309 618
Size: 691 3265
BsaBI GATnn'nnATC
Cuts at: 6 1470 3507 6
Size: 1464 2037 455
Fragments arranged by size:
2037 1464 455
BsaHI Gr'CG_yC
Cuts at: 121 759 1387 1635 2337 3833 121
Size: 638 628 248 702 1496 244
Fragments arranged by size:
1496 702 638 628 248 244
BsaWI w'CCGG_w
Cuts at: 719 1145 1666 2344 2618 2765 3176 719
Size: 426 521 678 274 147 411 1499
Fragments arranged by size:
1499 678 521 426 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 202 232 376 406 202
Size: 30 144 30 3752
Fragments arranged by size:
3752 144 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 718 847 718
Size: 129 3827
BseYI C'CCAG_C
Cuts at: 185 402 891 1381 2139 2716 3212 185
Size: 217 489 490 758 577 496 929
Fragments arranged by size:
929 758 577 496 490 489 217
BsiEI CG_ry'CG
Cuts at: 104 1498 1544 2752 104
Size: 1394 46 1208 1308
Fragments arranged by size:
1394 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 608 803 970 1748 1938 2730 608
Size: 195 167 778 190 792 1834
Fragments arranged by size:
1834 792 778 195 190 167
BsmBI CGTCTCn'nnnn_
Cuts at: 631 631
Size: 3956
Bsp1286I G_dGCh'C
Cuts at: 533 608 778 803 970 1581 1674 1748 1938
Size: 75 170 25 167 611 93 74 190
Cuts at: 1938 2000 2730 533
Size: 62 730 1759
Fragments arranged by size:
1759 730 611 190 170 167 93 75
74 62 25
BspEI T'CCGG_A
Cuts at: 3176 3176
Size: 3956
BspHI T'CATG_A
Cuts at: 3132 3132
Size: 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1006 1220 1522 1903 1006
Size: 214 302 381 3059
Fragments arranged by size:
3059 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2248 2302 3786 3951 50
Size: 2198 54 1484 165 55
Fragments arranged by size:
2198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 916 1868 916
Size: 952 3004
BsrFI r'CCGG_y
Cuts at: 719 1954 2135 2344 719
Size: 1235 181 209 2331
Fragments arranged by size:
2331 1235 209 181
BssSI C'ACGA_G
Cuts at: 2227 2585 2227
Size: 358 3598
BstAPI GCAn_nnn'nTGC
Cuts at: 1333 1333
Size: 3956
BstBI TT'CG_AA
Cuts at: 2317 2317
Size: 3956
BstYI r'GATC_y
Cuts at: 1 485 981 1448 1471 1806 2052 3064 3150
Size: 484 496 467 23 335 246 1012 86
Cuts at: 3150 3162 3508 1
Size: 12 346 449
Fragments arranged by size:
1012 496 484 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 221 107
Size: 114 3842
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1906 2087 2250 1906
Size: 181 163 3612
Fragments arranged by size:
3612 181 163
BtsI GCAGTG_nn'
Cuts at: 1199 1199
Size: 3956
DraI TTT'AAA
Cuts at: 955 955
Size: 3956
DraIII CAC_nnn'GTG
Cuts at: 236 236
Size: 3956
DrdI GACnn_nn'nnGTC
Cuts at: 1662 2520 1662
Size: 858 3098
EaeI y'GGCC_r
Cuts at: 110 248 341 551 1541 1715 2106 2133 3478
Size: 138 93 210 990 174 391 27 1345
Cuts at: 3478 110
Size: 588
Fragments arranged by size:
1345 990 588 391 210 174 138 93
27
EagI C'GGCC_G
Cuts at: 1541 1541
Size: 3956
EarI CTCTTCn'nnn_
Cuts at: 1979 2189 1979
Size: 210 3746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2164 2474 2620 3766 2164
Size: 310 146 1146 2354
Fragments arranged by size:
2354 1146 310 146
EcoICRI GAG'CTC
Cuts at: 606 968 606
Size: 362 3594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 403 466 592 607 916 1781 2213 2960
Size: 75 63 126 15 309 865 432 747
Cuts at: 2960 3228 328
Size: 268 1056
Fragments arranged by size:
1056 865 747 432 309 268 126 75
63 15
EcoO109I rG'GnC_Cy
Cuts at: 132 407 519 852 1095 132
Size: 275 112 333 243 2993
Fragments arranged by size:
2993 333 275 243 112
EcoRI G'AATT_C
Cuts at: 960 960
Size: 3956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1975 1975
Size: 3956
FspI TGC'GCA
Cuts at: 469 1737 469
Size: 1268 2688
HaeII r_GCGC'y
Cuts at: 124 1390 1638 2660 3639 124
Size: 1266 248 1022 979 441
Fragments arranged by size:
1266 1022 979 441 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1798 1828 1830 1860 1798
Size: 30 2 30 3894
Fragments arranged by size:
3894 30 30 2
HincII GTy'rAC
Cuts at: 995 995
Size: 3956
KasI G'GCGC_C
Cuts at: 120 1386 1634 120
Size: 1266 248 2442
Fragments arranged by size:
2442 1266 248
KpnI G_GTAC'C
Cuts at: 976 976
Size: 3956
MluI A'CGCG_T
Cuts at: 3389 3389
Size: 3956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2811 2627
Size: 184 3772
MscI TGG'CCA
Cuts at: 1717 1717
Size: 3956
MslI CAynn'nnrTG
Cuts at: 1945 2072 1945
Size: 127 3829
MspA1I CmG'CkG
Cuts at: 193 361 381 1067 1381 1741 2754 2999 193
Size: 168 20 686 314 360 1013 245 1150
Fragments arranged by size:
1150 1013 686 360 314 245 168 20
NaeI GCC'GGC
Cuts at: 2137 2137
Size: 3956
NarI GG'CG_CC
Cuts at: 121 1387 1635 121
Size: 1266 248 2442
Fragments arranged by size:
2442 1266 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3956
NgoMIV G'CCGG_C
Cuts at: 2135 2135
Size: 3956
NspI r_CATG'y
Cuts at: 1009 1509 2416 1009
Size: 500 907 2549
Fragments arranged by size:
2549 907 500
PciI A'CATG_T
Cuts at: 2412 2412
Size: 3956
PmeI GTTT'AAAC
Cuts at: 955 955
Size: 3956
PmlI CAC'GTG
Cuts at: 618 618
Size: 3956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3924
PpuMI rG'GwC_Cy
Cuts at: 132 407 132
Size: 275 3681
PshAI GACnn'nnGTC
Cuts at: 164 643 164
Size: 479 3477
PstI C_TGCA'G
Cuts at: 419 437 1003 1688 419
Size: 18 566 685 2687
Fragments arranged by size:
2687 685 566 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3956
PvuII CAG'CTG
Cuts at: 193 361 381 1381 1741 193
Size: 168 20 1000 360 2408
Fragments arranged by size:
2408 1000 360 168 20
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 3956
SacI G_AGCT'C
Cuts at: 608 970 608
Size: 362 3594
SalI G'TCGA_C
Cuts at: 993 993
Size: 3956
SanDI GG'GwC_CC
Cuts at: 132 132
Size: 3956
SapI GCTCTTCn'nnn_
Cuts at: 1979 2189 1979
Size: 210 3746
SbfI CC_TGCA'GG
Cuts at: 1003 1003
Size: 3956
ScaI AGT'ACT
Cuts at: 3473 3473
Size: 3956
SfcI C'TryA_G
Cuts at: 33 415 433 999 1684 2677 2868 33
Size: 382 18 566 685 993 191 1121
Fragments arranged by size:
1121 993 685 566 382 191 18
SfoI GGC'GCC
Cuts at: 122 1388 1636 122
Size: 1266 248 2442
Fragments arranged by size:
2442 1266 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
SmaI CCC'GGG
Cuts at: 130 503 978 130
Size: 373 475 3108
Fragments arranged by size:
3108 475 373
SmlI C'TyrA_G
Cuts at: 1111 2026 2518 2780 1111
Size: 915 492 262 2287
Fragments arranged by size:
2287 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3956
SphI G_CATG'C
Cuts at: 1009 1509 1009
Size: 500 3456
StuI AGG'CCT
Cuts at: 262 262
Size: 3956
StyI C'CwwG_G
Cuts at: 107 263 524 763 1090 1444 107
Size: 156 261 239 327 354 2619
Fragments arranged by size:
2619 354 327 261 239 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1539 1672 2131 2340 1539
Size: 133 459 209 3155
Fragments arranged by size:
3155 459 209 133
TatI w'GTAC_w
Cuts at: 3471 3471
Size: 3956
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 376 408 3473 3505 376
Size: 32 3065 32 827
Fragments arranged by size:
3065 827 32 32
Tth111I GACn'n_nGTC
Cuts at: 1753 1753
Size: 3956
XbaI T'CTAG_A
Cuts at: 987 987
Size: 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 261 1451 261
Size: 1190 2766
XmaI C'CCGG_G
Cuts at: 128 501 976 128
Size: 373 475 3108
Fragments arranged by size:
3108 475 373
ZraI GAC'GTC
Cuts at: 760 760
Size: 3956
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PmlI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI