Restriction map for
pF1KE0689
(Circular) (Six-base) MAPSORT of: pF1KE0689.seq Check: 2120 from: 1 to: 3953
pF1KE0689 3953 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 991 991
Size: 3953
Acc65I G'GTAC_C
Cuts at: 969 969
Size: 3953
AclI AA'CG_TT
Cuts at: 3793 3793
Size: 3953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 1778 2210 2957 3225 263
Size: 1515 432 747 268 991
Fragments arranged by size:
1515 991 747 432 268
AflIII A'CryG_T
Cuts at: 1305 2409 3386 1305
Size: 1104 977 1872
Fragments arranged by size:
1872 1104 977
AgeI A'CCGG_T
Cuts at: 2341 2341
Size: 3953
AhdI GACnn_n'nnGTC
Cuts at: 705 705
Size: 3953
AlwNI CAG_nnn'CTG
Cuts at: 1258 2825 1258
Size: 1567 2386
ApaLI G'TGCA_C
Cuts at: 2723 2723
Size: 3953
ApoI r'AATT_y
Cuts at: 385 400 957 3306 3562 385
Size: 15 557 2349 256 776
Fragments arranged by size:
2349 776 557 256 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 973 973
Size: 3953
BamHI G'GATC_C
Cuts at: 150 725 978 150
Size: 575 253 3125
Fragments arranged by size:
3125 575 253
BanI G'GyrC_C
Cuts at: 601 969 1383 1631 1666 601
Size: 368 414 248 35 2888
Fragments arranged by size:
2888 414 368 248 35
BanII G_rGCy'C
Cuts at: 967 1997 967
Size: 1030 2923
BbeI G_GCGC'C
Cuts at: 1387 1635 1387
Size: 248 3705
BciVI GTATCCnnnnn_n'
Cuts at: 1851 2612 3953 1851
Size: 761 1341 1851
Fragments arranged by size:
1851 1341 761
BclI T'GATC_A
Cuts at: 1473 1473
Size: 3953
BglII A'GATC_T
Cuts at: 159 1468 3505 159
Size: 1309 2037 607
Fragments arranged by size:
2037 1309 607
BlpI GC'TnA_GC
Cuts at: 188 1065 188
Size: 877 3076
Bme1580I G_kGCm'C
Cuts at: 584 604 1578 1671 2727 584
Size: 20 974 93 1056 1810
Fragments arranged by size:
1810 1056 974 93 20
BmrI ACTGGGnnnn_n'
Cuts at: 1165 1246 1581 3725 1165
Size: 81 335 2144 1393
Fragments arranged by size:
2144 1393 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 3953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 181
Size: 3953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2008 2500 2798 1129
Size: 879 492 298 2284
Fragments arranged by size:
2284 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 295 3657 295
Size: 3362 591
BsaAI yAC'GTr
Cuts at: 1306 1306
Size: 3953
BsaBI GATnn'nnATC
Cuts at: 6 1467 3504 6
Size: 1461 2037 455
Fragments arranged by size:
2037 1461 455
BsaHI Gr'CG_yC
Cuts at: 1384 1632 2334 3830 1384
Size: 248 702 1496 1507
Fragments arranged by size:
1507 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1142 1663 2341 2615 2762 3173 1142
Size: 521 678 274 147 411 1922
Fragments arranged by size:
1922 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 623 653 623
Size: 30 3923
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 244
Size: 3953
BseYI C'CCAG_C
Cuts at: 1378 2136 2713 3209 1378
Size: 758 577 496 2122
Fragments arranged by size:
2122 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 655
Size: 3953
BsiEI CG_ry'CG
Cuts at: 104 934 1495 1541 2749 104
Size: 830 561 46 1208 1308
Fragments arranged by size:
1308 1208 830 561 46
BsiHKAI G_wGCw'C
Cuts at: 145 967 1745 1935 2727 145
Size: 822 778 190 792 1371
Fragments arranged by size:
1371 822 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 145 584 604 967 1578 1671 1745 1935 1997
Size: 439 20 363 611 93 74 190 62
Cuts at: 1997 2727 145
Size: 730 1371
Fragments arranged by size:
1371 730 611 439 363 190 93 74
62 20
BspEI T'CCGG_A
Cuts at: 3173 3173
Size: 3953
BspHI T'CATG_A
Cuts at: 3129 3129
Size: 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1003 1217 1519 1900 1003
Size: 214 302 381 3056
Fragments arranged by size:
3056 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2245 2299 3783 3948 50
Size: 2195 54 1484 165 55
Fragments arranged by size:
2195 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 303 554 1865 303
Size: 251 1311 2391
Fragments arranged by size:
2391 1311 251
BsrFI r'CCGG_y
Cuts at: 1951 2132 2341 1951
Size: 181 209 3563
Fragments arranged by size:
3563 209 181
BsrGI T'GTAC_A
Cuts at: 753 753
Size: 3953
BssSI C'ACGA_G
Cuts at: 2224 2582 2224
Size: 358 3595
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 1330
Size: 3953
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstEII G'GTnAC_C
Cuts at: 613 613
Size: 3953
BstYI r'GATC_y
Cuts at: 1 150 159 172 725 978 1445 1468 1803
Size: 149 9 13 553 253 467 23 335
Cuts at: 1803 2049 3061 3147 3159 3505 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 553 467 449 346 335 253 246
149 86 23 13 12 9
BtgI C'CryG_G
Cuts at: 107 107
Size: 3953
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 1196 1196
Size: 3953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 485 520 485
Size: 35 3918
DraI TTT'AAA
Cuts at: 384 945 952 384
Size: 561 7 3385
Fragments arranged by size:
3385 561 7
DrdI GACnn_nn'nnGTC
Cuts at: 1659 2517 1659
Size: 858 3095
EaeI y'GGCC_r
Cuts at: 1538 1712 2103 2130 3475 1538
Size: 174 391 27 1345 2016
Fragments arranged by size:
2016 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1538 1538
Size: 3953
EarI CTCTTCn'nnn_
Cuts at: 254 1976 2186 254
Size: 1722 210 2021
Fragments arranged by size:
2021 1722 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2161 2471 2617 3763 2161
Size: 310 146 1146 2351
Fragments arranged by size:
2351 1146 310 146
EcoICRI GAG'CTC
Cuts at: 965 965
Size: 3953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 263 1778 2210 2957 3225 181
Size: 82 1515 432 747 268 909
Fragments arranged by size:
1515 909 747 432 268 82
EcoO109I rG'GnC_Cy
Cuts at: 1092 1092
Size: 3953
EcoRI G'AATT_C
Cuts at: 400 957 400
Size: 557 3396
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 592 1972 592
Size: 1380 2573
FspI TGC'GCA
Cuts at: 1734 1734
Size: 3953
HaeII r_GCGC'y
Cuts at: 1387 1635 2657 3636 1387
Size: 248 1022 979 1704
Fragments arranged by size:
1704 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 453 485 623 655 691 723 804 836 912
Size: 32 138 32 36 32 81 32 76
Cuts at: 912 944 1795 1825 1827 1857 453
Size: 32 851 30 2 30 2549
Fragments arranged by size:
2549 851 138 81 76 36 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 992 992
Size: 3953
KasI G'GCGC_C
Cuts at: 1383 1631 1383
Size: 248 3705
KpnI G_GTAC'C
Cuts at: 973 973
Size: 3953
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2624 2808 2624
Size: 184 3769
MscI TGG'CCA
Cuts at: 1714 1714
Size: 3953
MslI CAynn'nnrTG
Cuts at: 356 472 547 677 1942 2069 356
Size: 116 75 130 1265 127 2240
Fragments arranged by size:
2240 1265 130 127 116 75
MspA1I CmG'CkG
Cuts at: 751 1064 1378 1738 2751 2996 751
Size: 313 314 360 1013 245 1708
Fragments arranged by size:
1708 1013 360 314 313 245
NaeI GCC'GGC
Cuts at: 2134 2134
Size: 3953
NarI GG'CG_CC
Cuts at: 1384 1632 1384
Size: 248 3705
NcoI C'CATG_G
Cuts at: 107 107
Size: 3953
NgoMIV G'CCGG_C
Cuts at: 2132 2132
Size: 3953
NspI r_CATG'y
Cuts at: 1006 1506 2413 1006
Size: 500 907 2546
Fragments arranged by size:
2546 907 500
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PflMI CCAn_nnn'nTGG
Cuts at: 204 204
Size: 3953
PfoI T'CCnGG_A
Cuts at: 197 197
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3921
PstI C_TGCA'G
Cuts at: 1000 1685 1000
Size: 685 3268
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 751 1378 1738 751
Size: 627 360 2966
Fragments arranged by size:
2966 627 360
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 967 967
Size: 3953
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SapI GCTCTTCn'nnn_
Cuts at: 254 1976 2186 254
Size: 1722 210 2021
Fragments arranged by size:
2021 1722 210
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 3470 3470
Size: 3953
SfcI C'TryA_G
Cuts at: 33 996 1681 2674 2865 33
Size: 963 685 993 191 1121
Fragments arranged by size:
1121 993 963 685 191
SfoI GGC'GCC
Cuts at: 1385 1633 1385
Size: 248 3705
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 975 975
Size: 3953
SmlI C'TyrA_G
Cuts at: 1108 2023 2515 2777 1108
Size: 915 492 262 2284
Fragments arranged by size:
2284 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
StuI AGG'CCT
Cuts at: 635 635
Size: 3953
StyI C'CwwG_G
Cuts at: 107 906 1087 1441 107
Size: 799 181 354 2619
Fragments arranged by size:
2619 799 354 181
SwaI ATTT'AAAT
Cuts at: 384 384
Size: 3953
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 160 920 1536 1669 2128 2337 160
Size: 760 616 133 459 209 1776
Fragments arranged by size:
1776 760 616 459 209 133
TatI w'GTAC_w
Cuts at: 314 753 3468 314
Size: 439 2715 799
Fragments arranged by size:
2715 799 439
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 135 167 193 225 3470 3502 135
Size: 32 26 32 3245 32 586
Fragments arranged by size:
3245 586 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 1750 1750
Size: 3953
XbaI T'CTAG_A
Cuts at: 126 984 126
Size: 858 3095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1448 1448
Size: 3953
XmaI C'CCGG_G
Cuts at: 973 973
Size: 3953
XmnI GAAnn'nnTTC
Cuts at: 248 691 719 248
Size: 443 28 3482
Fragments arranged by size:
3482 443 28
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BssHII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI XhoI ZraI