Restriction map for
pF1KE0686
(Circular) (Six-base) MAPSORT of: pF1KE0686.seq Check: 410 from: 1 to: 4313
pF1KE0686 4313 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1351 1351
Size: 4313
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1153 1279 2138 2570 3317 3585 376
Size: 777 126 859 432 747 268 1104
Fragments arranged by size:
1104 859 777 747 432 268 126
AflIII A'CryG_T
Cuts at: 842 1665 2769 3746 842
Size: 823 1104 977 1409
Fragments arranged by size:
1409 1104 977 823
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AhdI GACnn_n'nnGTC
Cuts at: 846 846
Size: 4313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 358 358
Size: 4313
AlwNI CAG_nnn'CTG
Cuts at: 315 636 1618 3185 315
Size: 321 982 1567 1443
Fragments arranged by size:
1567 1443 982 321
ApaI G_GGCC'C
Cuts at: 1284 1284
Size: 4313
ApaLI G'TGCA_C
Cuts at: 3083 3083
Size: 4313
ApoI r'AATT_y
Cuts at: 373 961 1317 3666 3922 373
Size: 588 356 2349 256 764
Fragments arranged by size:
2349 764 588 356 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 1277 1333 1277
Size: 56 4257
BamHI G'GATC_C
Cuts at: 1338 1338
Size: 4313
BanI G'GyrC_C
Cuts at: 141 1329 1743 1991 2026 141
Size: 1188 414 248 35 2428
Fragments arranged by size:
2428 1188 414 248 35
BanII G_rGCy'C
Cuts at: 136 1037 1284 1327 2357 136
Size: 901 247 43 1030 2092
Fragments arranged by size:
2092 1030 901 247 43
BbeI G_GCGC'C
Cuts at: 1747 1995 1747
Size: 248 4065
BbsI GAAGACnn'nnnn_
Cuts at: 940 940
Size: 4313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4279
BciVI GTATCCnnnnn_n'
Cuts at: 896 2211 2972 4313 896
Size: 1315 761 1341 896
Fragments arranged by size:
1341 1315 896 761
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BfrBI ATG'CAT
Cuts at: 1240 1240
Size: 4313
BglI GCCn_nnn'nGGC
Cuts at: 1288 1288
Size: 4313
BglII A'GATC_T
Cuts at: 347 1828 3865 347
Size: 1481 2037 795
Fragments arranged by size:
2037 1481 795
BlpI GC'TnA_GC
Cuts at: 1425 1425
Size: 4313
Bme1580I G_kGCm'C
Cuts at: 451 1284 1938 2031 3087 451
Size: 833 654 93 1056 1677
Fragments arranged by size:
1677 1056 833 654 93
BmgBI CAC'GTC
Cuts at: 249 249
Size: 4313
BmrI ACTGGGnnnn_n'
Cuts at: 438 1525 1606 1941 4085 438
Size: 1087 81 335 2144 666
Fragments arranged by size:
2144 1087 666 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 785 1306 785
Size: 521 3792
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1489 2368 2860 3158 1489
Size: 879 492 298 2644
Fragments arranged by size:
2644 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 563 4017 563
Size: 3454 859
BsaAI yAC'GTr
Cuts at: 1666 1666
Size: 4313
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 1744 1992 2694 4190 1744
Size: 248 702 1496 1867
Fragments arranged by size:
1867 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1502 2023 2701 2975 3122 3533 1502
Size: 521 678 274 147 411 2282
Fragments arranged by size:
2282 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 581 1183 581
Size: 602 3711
BseYI C'CCAG_C
Cuts at: 321 810 1738 2496 3073 3569 321
Size: 489 928 758 577 496 1065
Fragments arranged by size:
1065 928 758 577 496 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1099 412
Size: 687 3626
BsiEI CG_ry'CG
Cuts at: 104 1855 1901 3109 104
Size: 1751 46 1208 1308
Fragments arranged by size:
1751 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1327 2105 2295 3087 1327
Size: 778 190 792 2553
Fragments arranged by size:
2553 792 778 190
BsiWI C'GTAC_G
Cuts at: 459 459
Size: 4313
BsmI GAATG_Cn'
Cuts at: 577 639 577
Size: 62 4251
Bsp1286I G_dGCh'C
Cuts at: 136 451 1037 1284 1327 1938 2031 2105 2295
Size: 315 586 247 43 611 93 74 190
Cuts at: 2295 2357 3087 136
Size: 62 730 1362
Fragments arranged by size:
1362 730 611 586 315 247 190 93
74 62 43
BspEI T'CCGG_A
Cuts at: 3533 3533
Size: 4313
BspHI T'CATG_A
Cuts at: 605 3489 605
Size: 2884 1429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1363 1577 1879 2260 1363
Size: 214 302 381 3416
Fragments arranged by size:
3416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1149 2605 2659 4143 4308 50
Size: 1099 1456 54 1484 165 55
Fragments arranged by size:
1484 1456 1099 165 55 54
BsrDI GCAATG_nn'
Cuts at: 650 2225 650
Size: 1575 2738
BsrFI r'CCGG_y
Cuts at: 138 2311 2492 2701 138
Size: 2173 181 209 1750
Fragments arranged by size:
2173 1750 209 181
BsrGI T'GTAC_A
Cuts at: 866 866
Size: 4313
BssSI C'ACGA_G
Cuts at: 2584 2942 2584
Size: 358 3955
BstAPI GCAn_nnn'nTGC
Cuts at: 1690 1690
Size: 4313
BstBI TT'CG_AA
Cuts at: 2674 2674
Size: 4313
BstEII G'GTnAC_C
Cuts at: 300 300
Size: 4313
BstXI CCAn_nnnn'nTGG
Cuts at: 1185 1185
Size: 4313
BstYI r'GATC_y
Cuts at: 1 347 1338 1805 1828 2163 2409 3421 3507
Size: 346 991 467 23 335 246 1012 86
Cuts at: 3507 3519 3865 1
Size: 12 346 449
Fragments arranged by size:
1012 991 467 449 346 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 906 906
Size: 4313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2263 2444 2607 2263
Size: 181 163 3969
Fragments arranged by size:
3969 181 163
BtsI GCAGTG_nn'
Cuts at: 1111 1556 1111
Size: 445 3868
ClaI AT'CG_AT
Cuts at: 287 287
Size: 4313
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 298 2019 2877 298
Size: 1721 858 1734
Fragments arranged by size:
1734 1721 858
EaeI y'GGCC_r
Cuts at: 1187 1898 2072 2463 2490 3835 1187
Size: 711 174 391 27 1345 1665
Fragments arranged by size:
1665 1345 711 391 174 27
EagI C'GGCC_G
Cuts at: 1898 1898
Size: 4313
EarI CTCTTCn'nnn_
Cuts at: 604 1255 2336 2546 604
Size: 651 1081 210 2371
Fragments arranged by size:
2371 1081 651 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1281 2521 2831 2977 4123 1281
Size: 1240 310 146 1146 1471
Fragments arranged by size:
1471 1240 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1325 1325
Size: 4313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 785 1153 1279 1306 2138 2570 3317 3585
Size: 409 368 126 27 832 432 747 268
Cuts at: 3585 376
Size: 1104
Fragments arranged by size:
1104 832 747 432 409 368 268 126
27
EcoNI CCTnn'n_nnAGG
Cuts at: 696 1135 696
Size: 439 3874
EcoO109I rG'GnC_Cy
Cuts at: 312 362 1098 1128 1281 1452 312
Size: 50 736 30 153 171 3173
Fragments arranged by size:
3173 736 171 153 50 30
EcoRI G'AATT_C
Cuts at: 1317 1317
Size: 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 1747 1995 3017 3996 1747
Size: 248 1022 979 2064
Fragments arranged by size:
2064 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 832 864 2155 2185 2187 2217 832
Size: 32 1291 30 2 30 2928
Fragments arranged by size:
2928 1291 32 30 30 2
HincII GTy'rAC
Cuts at: 1203 1352 1203
Size: 149 4164
KasI G'GCGC_C
Cuts at: 1743 1991 1743
Size: 248 4065
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 926 1268 2984 3168 140
Size: 786 342 1716 184 1285
Fragments arranged by size:
1716 1285 786 342 184
MscI TGG'CCA
Cuts at: 1189 2074 1189
Size: 885 3428
MslI CAynn'nnrTG
Cuts at: 662 946 2302 2429 662
Size: 284 1356 127 2546
Fragments arranged by size:
2546 1356 284 127
MspA1I CmG'CkG
Cuts at: 1424 1738 2098 3111 3356 1424
Size: 314 360 1013 245 2381
Fragments arranged by size:
2381 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2494 2494
Size: 4313
NarI GG'CG_CC
Cuts at: 1744 1992 1744
Size: 248 4065
NgoMIV G'CCGG_C
Cuts at: 2492 2492
Size: 4313
NsiI A_TGCA'T
Cuts at: 1242 1242
Size: 4313
NspI r_CATG'y
Cuts at: 846 1366 1866 2773 846
Size: 520 500 907 2386
Fragments arranged by size:
2386 907 520 500
PasI CC'CwG_GG
Cuts at: 123 852 123
Size: 729 3584
PciI A'CATG_T
Cuts at: 842 2769 842
Size: 1927 2386
PflMI CCAn_nnn'nTGG
Cuts at: 315 315
Size: 4313
PfoI T'CCnGG_A
Cuts at: 557 557
Size: 4313
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 312 362 1098 1128 312
Size: 50 736 30 3497
Fragments arranged by size:
3497 736 50 30
PspOMI G'GGCC_C
Cuts at: 1280 1280
Size: 4313
PstI C_TGCA'G
Cuts at: 266 1360 2045 266
Size: 1094 685 2534
Fragments arranged by size:
2534 1094 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 1738 2098 1738
Size: 360 3953
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 1327 1327
Size: 4313
SalI G'TCGA_C
Cuts at: 1350 1350
Size: 4313
SapI GCTCTTCn'nnn_
Cuts at: 604 2336 2546 604
Size: 1732 210 2371
Fragments arranged by size:
2371 1732 210
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SfcI C'TryA_G
Cuts at: 33 262 1356 2041 3034 3225 33
Size: 229 1094 685 993 191 1121
Fragments arranged by size:
1121 1094 993 685 229 191
SfoI GGC'GCC
Cuts at: 1745 1993 1745
Size: 248 4065
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SgrAI Cr'CCGG_yG
Cuts at: 138 138
Size: 4313
SmaI CCC'GGG
Cuts at: 1335 1335
Size: 4313
SmlI C'TyrA_G
Cuts at: 1468 2383 2875 3137 1468
Size: 915 492 262 2644
Fragments arranged by size:
2644 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
StyI C'CwwG_G
Cuts at: 404 740 1075 1184 1447 1801 404
Size: 336 335 109 263 354 2916
Fragments arranged by size:
2916 354 336 335 263 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 844 1054 1896 2029 2488 2697 844
Size: 210 842 133 459 209 2460
Fragments arranged by size:
2460 842 459 210 209 133
TatI w'GTAC_w
Cuts at: 866 3828 866
Size: 2962 1351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 238 270 3830 3862 238
Size: 32 3560 32 689
Fragments arranged by size:
3560 689 32 32
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 312 1808 312
Size: 1496 2817
XmaI C'CCGG_G
Cuts at: 1333 1333
Size: 4313
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbvCI BmtI Bpu10I BsaXI BsmBI BssHII BstZ17I
Bsu36I CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI PacI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI