Restriction map for
pF1KE0683
(Circular) (Six-base) MAPSORT of: pF1KE0683.seq Check: 4639 from: 1 to: 3935
pF1KE0683 3935 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 973 973
Size: 3935
Acc65I G'GTAC_C
Cuts at: 951 951
Size: 3935
AclI AA'CG_TT
Cuts at: 3775 3775
Size: 3935
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 892 1760 2192 2939 3207 448
Size: 444 868 432 747 268 1176
Fragments arranged by size:
1176 868 747 444 432 268
AflIII A'CryG_T
Cuts at: 1287 2391 3368 1287
Size: 1104 977 1854
Fragments arranged by size:
1854 1104 977
AgeI A'CCGG_T
Cuts at: 2323 2323
Size: 3935
AleI CACnn'nnGTG
Cuts at: 163 163
Size: 3935
AlwNI CAG_nnn'CTG
Cuts at: 426 1240 2807 426
Size: 814 1567 1554
Fragments arranged by size:
1567 1554 814
ApaI G_GGCC'C
Cuts at: 213 213
Size: 3935
ApaLI G'TGCA_C
Cuts at: 2705 2705
Size: 3935
ApoI r'AATT_y
Cuts at: 939 3288 3544 939
Size: 2349 256 1330
Fragments arranged by size:
2349 1330 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3935
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
AvaI C'yCGr_G
Cuts at: 161 212 955 161
Size: 51 743 3141
Fragments arranged by size:
3141 743 51
BamHI G'GATC_C
Cuts at: 960 960
Size: 3935
BanI G'GyrC_C
Cuts at: 287 519 641 951 1365 1613 1648 287
Size: 232 122 310 414 248 35 2574
Fragments arranged by size:
2574 414 310 248 232 122 35
BanII G_rGCy'C
Cuts at: 213 882 949 1979 213
Size: 669 67 1030 2169
Fragments arranged by size:
2169 1030 669 67
BbeI G_GCGC'C
Cuts at: 1369 1617 1369
Size: 248 3687
BciVI GTATCCnnnnn_n'
Cuts at: 540 1833 2594 3935 540
Size: 1293 761 1341 540
Fragments arranged by size:
1341 1293 761 540
BclI T'GATC_A
Cuts at: 1455 1455
Size: 3935
BfrBI ATG'CAT
Cuts at: 786 786
Size: 3935
BglI GCCn_nnn'nGGC
Cuts at: 354 354
Size: 3935
BglII A'GATC_T
Cuts at: 258 675 1450 3487 258
Size: 417 775 2037 706
Fragments arranged by size:
2037 775 706 417
BlpI GC'TnA_GC
Cuts at: 1047 1047
Size: 3935
Bme1580I G_kGCm'C
Cuts at: 213 290 524 872 1560 1653 2709 213
Size: 77 234 348 688 93 1056 1439
Fragments arranged by size:
1439 1056 688 348 234 93 77
BmrI ACTGGGnnnn_n'
Cuts at: 319 1147 1228 1563 3707 319
Size: 828 81 335 2144 547
Fragments arranged by size:
2144 828 547 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 3935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 866 1111 1990 2482 2780 457
Size: 409 245 879 492 298 1612
Fragments arranged by size:
1612 879 492 409 298 245
BsaI GGTCTCn'nnnn_
Cuts at: 3639 3639
Size: 3935
BsaAI yAC'GTr
Cuts at: 867 1288 867
Size: 421 3514
BsaBI GATnn'nnATC
Cuts at: 6 1449 3486 6
Size: 1443 2037 455
Fragments arranged by size:
2037 1443 455
BsaHI Gr'CG_yC
Cuts at: 1366 1614 2316 3812 1366
Size: 248 702 1496 1489
Fragments arranged by size:
1496 1489 702 248
BsaWI w'CCGG_w
Cuts at: 1124 1645 2323 2597 2744 3155 1124
Size: 521 678 274 147 411 1904
Fragments arranged by size:
1904 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 583
Size: 3935
BseYI C'CCAG_C
Cuts at: 143 1360 2118 2695 3191 143
Size: 1217 758 577 496 887
Fragments arranged by size:
1217 887 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 283
Size: 3935
BsiEI CG_ry'CG
Cuts at: 104 1477 1523 2731 104
Size: 1373 46 1208 1308
Fragments arranged by size:
1373 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 882 949 1727 1917 2709 882
Size: 67 778 190 792 2108
Fragments arranged by size:
2108 792 778 190 67
BsmBI CGTCTCn'nnnn_
Cuts at: 733 733
Size: 3935
Bsp1286I G_dGCh'C
Cuts at: 213 290 524 872 882 949 1560 1653 1727
Size: 77 234 348 10 67 611 93 74
Cuts at: 1727 1917 1979 2709 213
Size: 190 62 730 1439
Fragments arranged by size:
1439 730 611 348 234 190 93 77
74 67 62 10
BspEI T'CCGG_A
Cuts at: 3155 3155
Size: 3935
BspHI T'CATG_A
Cuts at: 3111 3111
Size: 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 985 1199 1501 1882 985
Size: 214 302 381 3038
Fragments arranged by size:
3038 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2227 2281 3765 3930 50
Size: 2177 54 1484 165 55
Fragments arranged by size:
2177 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1847 1847
Size: 3935
BsrFI r'CCGG_y
Cuts at: 1933 2114 2323 1933
Size: 181 209 3545
Fragments arranged by size:
3545 209 181
BsrGI T'GTAC_A
Cuts at: 606 606
Size: 3935
BssSI C'ACGA_G
Cuts at: 442 2206 2564 442
Size: 1764 358 1813
Fragments arranged by size:
1813 1764 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1312 1312
Size: 3935
BstBI TT'CG_AA
Cuts at: 2296 2296
Size: 3935
BstYI r'GATC_y
Cuts at: 1 258 675 960 1427 1450 1785 2031 3043
Size: 257 417 285 467 23 335 246 1012
Cuts at: 3043 3129 3141 3487 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 417 346 335 285 257
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 713 713
Size: 3935
BtgI C'CryG_G
Cuts at: 107 107
Size: 3935
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1885 2066 2229 1885
Size: 181 163 3591
Fragments arranged by size:
3591 181 163
BtsI GCAGTG_nn'
Cuts at: 515 565 1178 515
Size: 50 613 3272
Fragments arranged by size:
3272 613 50
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 168 203 168
Size: 35 3900
DraI TTT'AAA
Cuts at: 934 934
Size: 3935
DrdI GACnn_nn'nnGTC
Cuts at: 1641 2499 1641
Size: 858 3077
EaeI y'GGCC_r
Cuts at: 355 1520 1694 2085 2112 3457 355
Size: 1165 174 391 27 1345 833
Fragments arranged by size:
1345 1165 833 391 174 27
EagI C'GGCC_G
Cuts at: 1520 1520
Size: 3935
EarI CTCTTCn'nnn_
Cuts at: 217 911 1958 2168 217
Size: 694 1047 210 1984
Fragments arranged by size:
1984 1047 694 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2143 2453 2599 3745 2143
Size: 310 146 1146 2333
Fragments arranged by size:
2333 1146 310 146
EcoICRI GAG'CTC
Cuts at: 880 947 880
Size: 67 3868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 892 1760 2192 2939 3207 448
Size: 444 868 432 747 268 1176
Fragments arranged by size:
1176 868 747 444 432 268
EcoO109I rG'GnC_Cy
Cuts at: 202 534 1074 202
Size: 332 540 3063
Fragments arranged by size:
3063 540 332
EcoRI G'AATT_C
Cuts at: 939 939
Size: 3935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1954 1954
Size: 3935
FspI TGC'GCA
Cuts at: 1716 1716
Size: 3935
HaeII r_GCGC'y
Cuts at: 1369 1617 2639 3618 1369
Size: 248 1022 979 1686
Fragments arranged by size:
1686 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 582 614 667 699 1777 1807 1809 1839 582
Size: 32 53 32 1078 30 2 30 2678
Fragments arranged by size:
2678 1078 53 32 32 30 30 2
HincII GTy'rAC
Cuts at: 974 974
Size: 3935
KasI G'GCGC_C
Cuts at: 1365 1613 1365
Size: 248 3687
KpnI G_GTAC'C
Cuts at: 955 955
Size: 3935
MluI A'CGCG_T
Cuts at: 3368 3368
Size: 3935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2606 2790 2606
Size: 184 3751
MscI TGG'CCA
Cuts at: 357 1696 357
Size: 1339 2596
MslI CAynn'nnrTG
Cuts at: 163 791 1924 2051 163
Size: 628 1133 127 2047
Fragments arranged by size:
2047 1133 628 127
MspA1I CmG'CkG
Cuts at: 426 626 1046 1360 1720 2733 2978 426
Size: 200 420 314 360 1013 245 1383
Fragments arranged by size:
1383 1013 420 360 314 245 200
NaeI GCC'GGC
Cuts at: 2116 2116
Size: 3935
NarI GG'CG_CC
Cuts at: 1366 1614 1366
Size: 248 3687
NcoI C'CATG_G
Cuts at: 107 107
Size: 3935
NgoMIV G'CCGG_C
Cuts at: 2114 2114
Size: 3935
NsiI A_TGCA'T
Cuts at: 788 788
Size: 3935
NspI r_CATG'y
Cuts at: 988 1488 2395 988
Size: 500 907 2528
Fragments arranged by size:
2528 907 500
PasI CC'CwG_GG
Cuts at: 206 873 206
Size: 667 3268
PciI A'CATG_T
Cuts at: 2391 2391
Size: 3935
PflMI CCAn_nnn'nTGG
Cuts at: 774 774
Size: 3935
PmeI GTTT'AAAC
Cuts at: 934 934
Size: 3935
PmlI CAC'GTG
Cuts at: 867 867
Size: 3935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 3903
PpuMI rG'GwC_Cy
Cuts at: 202 534 202
Size: 332 3603
PshAI GACnn'nnGTC
Cuts at: 851 851
Size: 3935
PspOMI G'GGCC_C
Cuts at: 209 209
Size: 3935
PstI C_TGCA'G
Cuts at: 670 982 1667 670
Size: 312 685 2938
Fragments arranged by size:
2938 685 312
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3935
PvuII CAG'CTG
Cuts at: 426 626 1360 1720 426
Size: 200 734 360 2641
Fragments arranged by size:
2641 734 360 200
RsrII CG'GwC_CG
Cuts at: 2130 2130
Size: 3935
SacI G_AGCT'C
Cuts at: 882 949 882
Size: 67 3868
SalI G'TCGA_C
Cuts at: 972 972
Size: 3935
SanDI GG'GwC_CC
Cuts at: 202 202
Size: 3935
SapI GCTCTTCn'nnn_
Cuts at: 217 1958 2168 217
Size: 1741 210 1984
Fragments arranged by size:
1984 1741 210
SbfI CC_TGCA'GG
Cuts at: 982 982
Size: 3935
ScaI AGT'ACT
Cuts at: 329 3452 329
Size: 3123 812
SfcI C'TryA_G
Cuts at: 33 538 666 978 1663 2656 2847 33
Size: 505 128 312 685 993 191 1121
Fragments arranged by size:
1121 993 685 505 312 191 128
SfoI GGC'GCC
Cuts at: 1367 1615 1367
Size: 248 3687
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
SmaI CCC'GGG
Cuts at: 163 957 163
Size: 794 3141
SmlI C'TyrA_G
Cuts at: 472 881 1090 2005 2497 2759 472
Size: 409 209 915 492 262 1648
Fragments arranged by size:
1648 915 492 409 262 209
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3935
SphI G_CATG'C
Cuts at: 988 1488 988
Size: 500 3435
SspI AAT'ATT
Cuts at: 810 810
Size: 3935
StuI AGG'CCT
Cuts at: 440 440
Size: 3935
StyI C'CwwG_G
Cuts at: 107 524 1069 1423 107
Size: 417 545 354 2619
Fragments arranged by size:
2619 545 417 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 157 505 907 1518 1651 2110 2319 157
Size: 348 402 611 133 459 209 1773
Fragments arranged by size:
1773 611 459 402 348 209 133
TatI w'GTAC_w
Cuts at: 327 606 680 911 3450 327
Size: 279 74 231 2539 812
Fragments arranged by size:
2539 812 279 231 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 292 324 3452 3484 292
Size: 32 3128 32 743
Fragments arranged by size:
3128 743 32 32
Tth111I GACn'n_nGTC
Cuts at: 1732 1732
Size: 3935
XbaI T'CTAG_A
Cuts at: 816 966 816
Size: 150 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1430 1430
Size: 3935
XmaI C'CCGG_G
Cuts at: 161 955 161
Size: 794 3141
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI BpmI
Bpu10I BsaXI BsiWI BsmI BssHII BstEII BstXI BstZ17I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PfoI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI