Restriction map for
pF1KE0674
(Circular) (Six-base) MAPSORT of: pF1KE0674.seq Check: 2969 from: 1 to: 4301
pF1KE0674 4301 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1182 1339 1182
Size: 157 4144
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 437 555 975 2126 2558 3305 3573 285
Size: 152 118 420 1151 432 747 268 1013
Fragments arranged by size:
1151 1013 747 432 420 268 152 118
AflIII A'CryG_T
Cuts at: 1653 2757 3734 1653
Size: 1104 977 2220
Fragments arranged by size:
2220 1104 977
AgeI A'CCGG_T
Cuts at: 2689 2689
Size: 4301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 706 706
Size: 4301
AlwNI CAG_nnn'CTG
Cuts at: 1606 3173 1606
Size: 1567 2734
ApaLI G'TGCA_C
Cuts at: 3071 3071
Size: 4301
ApoI r'AATT_y
Cuts at: 1305 3654 3910 1305
Size: 2349 256 1696
Fragments arranged by size:
2349 1696 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 1321 1321
Size: 4301
BamHI G'GATC_C
Cuts at: 1326 1326
Size: 4301
BanI G'GyrC_C
Cuts at: 1317 1731 1979 2014 1317
Size: 414 248 35 3604
Fragments arranged by size:
3604 414 248 35
BanII G_rGCy'C
Cuts at: 1315 2345 1315
Size: 1030 3271
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BbsI GAAGACnn'nnnn_
Cuts at: 347 684 347
Size: 337 3964
BciVI GTATCCnnnnn_n'
Cuts at: 1175 2199 2960 4301 1175
Size: 1024 761 1341 1175
Fragments arranged by size:
1341 1175 1024 761
BclI T'GATC_A
Cuts at: 1821 1821
Size: 4301
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 1413 1413
Size: 4301
Bme1580I G_kGCm'C
Cuts at: 506 1926 2019 3075 506
Size: 1420 93 1056 1732
Fragments arranged by size:
1732 1420 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 959 1043 1163 1513 1594 1929 4073 959
Size: 84 120 350 81 335 2144 1187
Fragments arranged by size:
2144 1187 350 335 120 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 887 1302 803
Size: 84 415 3802
Fragments arranged by size:
3802 415 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1477 2356 2848 3146 1477
Size: 879 492 298 2632
Fragments arranged by size:
2632 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4005 4005
Size: 4301
BsaAI yAC'GTr
Cuts at: 1163 1654 1163
Size: 491 3810
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 1732 1980 2682 4178 1732
Size: 248 702 1496 1855
Fragments arranged by size:
1855 1496 702 248
BsaWI w'CCGG_w
Cuts at: 613 1490 2011 2689 2963 3110 3521 613
Size: 877 521 678 274 147 411 1393
Fragments arranged by size:
1393 877 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 4301
BseYI C'CCAG_C
Cuts at: 1726 2484 3061 3557 1726
Size: 758 577 496 2470
Fragments arranged by size:
2470 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3097 104
Size: 1739 46 1208 1308
Fragments arranged by size:
1739 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1315 2093 2283 3075 1315
Size: 778 190 792 2541
Fragments arranged by size:
2541 792 778 190
BsmI GAATG_Cn'
Cuts at: 671 904 1072 1156 1278 671
Size: 233 168 84 122 3694
Fragments arranged by size:
3694 233 168 122 84
Bsp1286I G_dGCh'C
Cuts at: 506 1315 1926 2019 2093 2283 2345 3075 506
Size: 809 611 93 74 190 62 730 1732
Fragments arranged by size:
1732 809 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3521 3521
Size: 4301
BspHI T'CATG_A
Cuts at: 3477 3477
Size: 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1351 1565 1867 2248 1351
Size: 214 302 381 3404
Fragments arranged by size:
3404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 898 2213 898
Size: 1315 2986
BsrFI r'CCGG_y
Cuts at: 2299 2480 2689 2299
Size: 181 209 3911
Fragments arranged by size:
3911 209 181
BssSI C'ACGA_G
Cuts at: 2572 2930 2572
Size: 358 3943
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 675 2662 675
Size: 1987 2314
BstYI r'GATC_y
Cuts at: 1 1087 1326 1793 1816 2151 2397 3409 3495
Size: 1086 239 467 23 335 246 1012 86
Cuts at: 3495 3507 3853 1
Size: 12 346 449
Fragments arranged by size:
1086 1012 467 449 346 335 246 239
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2251 2432 2595 2251
Size: 181 163 3957
Fragments arranged by size:
3957 181 163
BtsI GCAGTG_nn'
Cuts at: 1234 1544 1234
Size: 310 3991
DraI TTT'AAA
Cuts at: 1300 1300
Size: 4301
DrdI GACnn_nn'nnGTC
Cuts at: 2007 2865 2007
Size: 858 3443
EaeI y'GGCC_r
Cuts at: 1886 2060 2451 2478 3823 1886
Size: 174 391 27 1345 2364
Fragments arranged by size:
2364 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 2324 2534 2324
Size: 210 4091
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2509 2819 2965 4111 2509
Size: 310 146 1146 2699
Fragments arranged by size:
2699 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1313 1313
Size: 4301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 437 555 803 887 975 1302 2126 2558
Size: 152 118 248 84 88 327 824 432
Cuts at: 2558 3305 3573 285
Size: 747 268 1013
Fragments arranged by size:
1013 824 747 432 327 268 248 152
118 88 84
EcoO109I rG'GnC_Cy
Cuts at: 117 1193 1440 117
Size: 1076 247 2978
Fragments arranged by size:
2978 1076 247
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
EcoRV GAT'ATC
Cuts at: 577 577
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 585 2320 585
Size: 1735 2566
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 1735 1983 3005 3984 1735
Size: 248 1022 979 2052
Fragments arranged by size:
2052 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2143 2173 2175 2205 2143
Size: 30 2 30 4239
Fragments arranged by size:
4239 30 30 2
HincII GTy'rAC
Cuts at: 553 1340 553
Size: 787 3514
HindIII A'AGCT_T
Cuts at: 407 407
Size: 4301
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2972 3156 2972
Size: 184 4117
MscI TGG'CCA
Cuts at: 2062 2062
Size: 4301
MslI CAynn'nnrTG
Cuts at: 546 688 966 2290 2417 546
Size: 142 278 1324 127 2430
Fragments arranged by size:
2430 1324 278 142 127
MspA1I CmG'CkG
Cuts at: 1412 1726 2086 3099 3344 1412
Size: 314 360 1013 245 2369
Fragments arranged by size:
2369 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2482 2482
Size: 4301
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NdeI CA'TA_TG
Cuts at: 963 963
Size: 4301
NgoMIV G'CCGG_C
Cuts at: 2480 2480
Size: 4301
NspI r_CATG'y
Cuts at: 1354 1854 2761 1354
Size: 500 907 2894
Fragments arranged by size:
2894 907 500
PciI A'CATG_T
Cuts at: 2757 2757
Size: 4301
PfoI T'CCnGG_A
Cuts at: 395 395
Size: 4301
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 220 252 3440 3472 220
Size: 32 3188 32 1049
Fragments arranged by size:
3188 1049 32 32
PpuMI rG'GwC_Cy
Cuts at: 117 1193 117
Size: 1076 3225
PsiI TTA'TAA
Cuts at: 713 1250 713
Size: 537 3764
PstI C_TGCA'G
Cuts at: 1348 2033 1348
Size: 685 3616
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 1726 2086 1726
Size: 360 3941
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 1315 1315
Size: 4301
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 2324 2534 2324
Size: 210 4091
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SfcI C'TryA_G
Cuts at: 33 198 239 1344 2029 3022 3213 33
Size: 165 41 1105 685 993 191 1121
Fragments arranged by size:
1121 1105 993 685 191 165 41
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 1323 1323
Size: 4301
SmlI C'TyrA_G
Cuts at: 1456 2371 2863 3125 1456
Size: 915 492 262 2632
Fragments arranged by size:
2632 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1354 1854 1354
Size: 500 3801
StyI C'CwwG_G
Cuts at: 1435 1789 1435
Size: 354 3947
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 169 1884 2017 2476 2685 169
Size: 1715 133 459 209 1785
Fragments arranged by size:
1785 1715 459 209 133
TatI w'GTAC_w
Cuts at: 3816 3816
Size: 4301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4269
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1796 1796
Size: 4301
XmaI C'CCGG_G
Cuts at: 1321 1321
Size: 4301
XmnI GAAnn'nnTTC
Cuts at: 568 904 568
Size: 336 3965
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI FseI FspAI HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI