Restriction map for pF1KE0672
 (Circular) (Six-base) MAPSORT of: pF1KE0672.seq  Check: 8961  from: 1  to: 4142



pF1KE0672  4142 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    895     895 
   Size:       4142
 
AccI GT'mk_AC

Cuts at:    149    1180     149 
   Size:       1031    3111
 
Acc65I G'GTAC_C

Cuts at:    731    1158     731 
   Size:        427    3715
 
AclI AA'CG_TT

Cuts at:   3982    3982 
   Size:       4142
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     753    1967    2399    3146    3414     139 
   Size:        614    1214     432     747     268     867

  Fragments arranged by size: 

                1214     867     747     614     432     268
 
AflIII A'CryG_T

Cuts at:   1494    2598    3575    1494 
   Size:       1104     977    2061

  Fragments arranged by size: 

                2061    1104     977
 
AgeI A'CCGG_T

Cuts at:   2530    2530 
   Size:       4142
 
AlwNI CAG_nnn'CTG

Cuts at:    780    1447    3014     780 
   Size:        667    1567    1908

  Fragments arranged by size: 

                1908    1567     667
 
ApaI G_GGCC'C

Cuts at:    699     699 
   Size:       4142
 
ApaLI G'TGCA_C

Cuts at:    505    2912     505 
   Size:       2407    1735
 
ApoI r'AATT_y

Cuts at:   1146    3495    3751    1146 
   Size:       2349     256    1537

  Fragments arranged by size: 

                2349    1537     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4142
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4142
 
AvaI C'yCGr_G

Cuts at:    174    1162     174 
   Size:        988    3154
 
BamHI G'GATC_C

Cuts at:   1167    1167 
   Size:       4142
 
BanI G'GyrC_C

Cuts at:    178     472     616     731     851     905    1158    1572    1820 
   Size:        294     144     115     120      54     253     414     248

Cuts at:   1820    1855     178 
   Size:         35    2465

  Fragments arranged by size: 

                2465     414     294     253     248     144     120     115
                  54      35
 
BanII G_rGCy'C

Cuts at:    217     355     656     699     767    1156    2186     217 
   Size:        138     301      43      68     389    1030    2173

  Fragments arranged by size: 

                2173    1030     389     301     138      68      43
 
BbeI G_GCGC'C

Cuts at:    476    1576    1824     476 
   Size:       1100     248    2794

  Fragments arranged by size: 

                2794    1100     248
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4108
 
BciVI GTATCCnnnnn_n'

Cuts at:   2040    2801    4142    2040 
   Size:        761    1341    2040

  Fragments arranged by size: 

                2040    1341     761
 
BclI T'GATC_A

Cuts at:   1662    1662 
   Size:       4142
 
BglI GCCn_nnn'nGGC

Cuts at:   1106    1106 
   Size:       4142
 
BglII A'GATC_T

Cuts at:   1657    3694    1657 
   Size:       2037    2105
 
BlpI GC'TnA_GC

Cuts at:   1254    1254 
   Size:       4142
 
Bme1580I G_kGCm'C

Cuts at:    509     699    1767    1860    2916     509 
   Size:        190    1068      93    1056    1735

  Fragments arranged by size: 

                1735    1068    1056     190      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1354    1435    1770    3914    1354 
   Size:         81     335    2144    1582

  Fragments arranged by size: 

                2144    1582     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    231     441     819    1089    2509     231 
   Size:        210     378     270    1420    1864

  Fragments arranged by size: 

                1864    1420     378     270     210
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    355     880     355 
   Size:        525    3617
 
Bpu10I CC'TnA_GC

Cuts at:    761     761 
   Size:       4142
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    220     502    1318    2197    2689    2987     220 
   Size:        282     816     879     492     298    1375

  Fragments arranged by size: 

                1375     879     816     492     298     282
 
BsaI GGTCTCn'nnnn_

Cuts at:    576     860    3846     576 
   Size:        284    2986     872

  Fragments arranged by size: 

                2986     872     284
 
BsaAI yAC'GTr

Cuts at:   1495    1495 
   Size:       4142
 
BsaBI GATnn'nnATC

Cuts at:      6    1656    3693       6 
   Size:       1650    2037     455

  Fragments arranged by size: 

                2037    1650     455
 
BsaHI Gr'CG_yC

Cuts at:    473     704    1573    1821    2523    4019     473 
   Size:        231     869     248     702    1496     596

  Fragments arranged by size: 

                1496     869     702     596     248     231
 
BsaWI w'CCGG_w

Cuts at:    971    1037    1331    1852    2530    2804    2951    3362     971 
   Size:         66     294     521     678     274     147     411    1751

  Fragments arranged by size: 

                1751     678     521     411     294     274     147      66
            
 
BseYI C'CCAG_C

Cuts at:    601    1044    1567    2325    2902    3398     601 
   Size:        443     523     758     577     496    1345

  Fragments arranged by size: 

                1345     758     577     523     496     443
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    472     472 
   Size:       4142
 
BsiEI CG_ry'CG

Cuts at:    104    1684    1730    2938     104 
   Size:       1580      46    1208    1308

  Fragments arranged by size: 

                1580    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    302     509     656     767    1156    1934    2124    2916     302 
   Size:        207     147     111     389     778     190     792    1528

  Fragments arranged by size: 

                1528     792     778     389     207     190     147     111
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:    483     483 
   Size:       4142
 
Bsp1286I G_dGCh'C

Cuts at:    217     302     355     509     656     699     767    1156    1767 
   Size:         85      53     154     147      43      68     389     611

Cuts at:   1767    1860    1934    2124    2186    2916     217 
   Size:         93      74     190      62     730    1443

  Fragments arranged by size: 

                1443     730     611     389     190     154     147      93
                  85      74      68      62      53      43
 
BspEI T'CCGG_A

Cuts at:    971    1037    3362     971 
   Size:         66    2325    1751

  Fragments arranged by size: 

                2325    1751      66
 
BspHI T'CATG_A

Cuts at:   3318    3318 
   Size:       4142
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    494     895    1192    1406    1708    2089     494 
   Size:        401     297     214     302     381    2547

  Fragments arranged by size: 

                2547     401     381     302     297     214
 
BsrBI CCG'CTC

Cuts at:     50     210    2434    2488    3972    4137      50 
   Size:        160    2224      54    1484     165      55

  Fragments arranged by size: 

                2224    1484     165     160      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2054    2054 
   Size:       4142
 
BsrFI r'CCGG_y

Cuts at:   2140    2321    2530    2140 
   Size:        181     209    3752

  Fragments arranged by size: 

                3752     209     181
 
BssSI C'ACGA_G

Cuts at:    226     346    2413    2771     226 
   Size:        120    2067     358    1597

  Fragments arranged by size: 

                2067    1597     358     120
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1519    1519 
   Size:       4142
 
BstBI TT'CG_AA

Cuts at:   2503    2503 
   Size:       4142
 
BstXI CCAn_nnnn'nTGG

Cuts at:    381     453     381 
   Size:         72    4070
 
BstYI r'GATC_y

Cuts at:      1     155     776     914     968    1052    1167    1634    1657 
   Size:        154     621     138      54      84     115     467      23

Cuts at:   1657    1992    2238    3250    3336    3348    3694       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     621     467     449     346     335     246     154
                 138     115      86      84      54      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    781     781 
   Size:       4142
 
BtgI C'CryG_G

Cuts at:    374     374 
   Size:       4142
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2092    2273    2436    2092 
   Size:        181     163    3798

  Fragments arranged by size: 

                3798     181     163
 
BtsI GCAGTG_nn'

Cuts at:    302    1385     302 
   Size:       1083    3059
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    531     566     531 
   Size:         35    4107
 
DraI TTT'AAA

Cuts at:   1141    1141 
   Size:       4142
 
DraIII CAC_nnn'GTG

Cuts at:    228     228 
   Size:       4142
 
DrdI GACnn_nn'nnGTC

Cuts at:   1848    2706    1848 
   Size:        858    3284
 
EaeI y'GGCC_r

Cuts at:    371    1119    1727    1901    2292    2319    3664     371 
   Size:        748     608     174     391      27    1345     849

  Fragments arranged by size: 

                1345     849     748     608     391     174      27
 
EagI C'GGCC_G

Cuts at:   1727    1727 
   Size:       4142
 
EarI CTCTTCn'nnn_

Cuts at:    772     871    2165    2375     772 
   Size:         99    1294     210    2539

  Fragments arranged by size: 

                2539    1294     210      99
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2350    2660    2806    3952    2350 
   Size:        310     146    1146    2540

  Fragments arranged by size: 

                2540    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    654     765    1154     654 
   Size:        111     389    3642

  Fragments arranged by size: 

                3642     389     111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     355     753     880    1967    2399    3146    3414     139 
   Size:        216     398     127    1087     432     747     268     867

  Fragments arranged by size: 

                1087     867     747     432     398     268     216     127
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    936     936 
   Size:       4142
 
EcoO109I rG'GnC_Cy

Cuts at:    255     273     465     513     629     696    1070    1281     255 
   Size:         18     192      48     116      67     374     211    3116

  Fragments arranged by size: 

                3116     374     211     192     116      67      48      18
            
 
EcoRI G'AATT_C

Cuts at:   1146    1146 
   Size:       4142
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1023    2161    1023 
   Size:       1138    3004
 
FspI TGC'GCA

Cuts at:   1923    1923 
   Size:       4142
 
HaeII r_GCGC'y

Cuts at:    476    1576    1824    2846    3825     476 
   Size:       1100     248    1022     979     793

  Fragments arranged by size: 

                1100    1022     979     793     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    808     840    1984    2014    2016    2046     808 
   Size:         32    1144      30       2      30    2904

  Fragments arranged by size: 

                2904    1144      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1181    1181 
   Size:       4142
 
KasI G'GCGC_C

Cuts at:    472    1572    1820     472 
   Size:       1100     248    2794

  Fragments arranged by size: 

                2794    1100     248
 
KpnI G_GTAC'C

Cuts at:    735    1162     735 
   Size:        427    3715
 
MluI A'CGCG_T

Cuts at:   3575    3575 
   Size:       4142
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2813    2997    2813 
   Size:        184    3958
 
MscI TGG'CCA

Cuts at:    373    1121    1903     373 
   Size:        748     782    2612

  Fragments arranged by size: 

                2612     782     748
 
MslI CAynn'nnrTG

Cuts at:   2131    2258    2131 
   Size:        127    4015
 
MspA1I CmG'CkG

Cuts at:   1253    1567    1927    2940    3185    1253 
   Size:        314     360    1013     245    2210

  Fragments arranged by size: 

                2210    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2323    2323 
   Size:       4142
 
NarI GG'CG_CC

Cuts at:    473    1573    1821     473 
   Size:       1100     248    2794

  Fragments arranged by size: 

                2794    1100     248
 
NcoI C'CATG_G

Cuts at:    374     374 
   Size:       4142
 
NgoMIV G'CCGG_C

Cuts at:   2321    2321 
   Size:       4142
 
NspI r_CATG'y

Cuts at:    612    1195    1695    2602     612 
   Size:        583     500     907    2152

  Fragments arranged by size: 

                2152     907     583     500
 
PasI CC'CwG_GG

Cuts at:    699     699 
   Size:       4142
 
PciI A'CATG_T

Cuts at:   2598    2598 
   Size:       4142
 
PflMI CCAn_nnn'nTGG

Cuts at:    456     456 
   Size:       4142
 
PmeI GTTT'AAAC

Cuts at:   1141    1141 
   Size:       4142
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3281    3313    3281 
   Size:         32    4110
 
PpuMI rG'GwC_Cy

Cuts at:    465     513     629     465 
   Size:         48     116    3978

  Fragments arranged by size: 

                3978     116      48
 
PspOMI G'GGCC_C

Cuts at:    695     695 
   Size:       4142
 
PstI C_TGCA'G

Cuts at:   1189    1874    1189 
   Size:        685    3457
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4142
 
PvuII CAG'CTG

Cuts at:   1567    1927    1567 
   Size:        360    3782
 
RsrII CG'GwC_CG

Cuts at:   2337    2337 
   Size:       4142
 
SacI G_AGCT'C

Cuts at:    656     767    1156     656 
   Size:        111     389    3642

  Fragments arranged by size: 

                3642     389     111
 
SalI G'TCGA_C

Cuts at:   1179    1179 
   Size:       4142
 
SanDI GG'GwC_CC

Cuts at:    629     629 
   Size:       4142
 
SapI GCTCTTCn'nnn_

Cuts at:    772    2165    2375     772 
   Size:       1393     210    2539

  Fragments arranged by size: 

                2539    1393     210
 
SbfI CC_TGCA'GG

Cuts at:   1189    1189 
   Size:       4142
 
ScaI AGT'ACT

Cuts at:   3659    3659 
   Size:       4142
 
SfcI C'TryA_G

Cuts at:     33    1185    1870    2863    3054      33 
   Size:       1152     685     993     191    1121

  Fragments arranged by size: 

                1152    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:    474    1574    1822     474 
   Size:       1100     248    2794

  Fragments arranged by size: 

                2794    1100     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4142
 
SmaI CCC'GGG

Cuts at:   1164    1164 
   Size:       4142
 
SmlI C'TyrA_G

Cuts at:    235     517    1297    2212    2704    2966     235 
   Size:        282     780     915     492     262    1411

  Fragments arranged by size: 

                1411     915     780     492     282     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4142
 
SphI G_CATG'C

Cuts at:   1195    1695    1195 
   Size:        500    3642
 
StuI AGG'CCT

Cuts at:    898    1109     898 
   Size:        211    3931
 
StyI C'CwwG_G

Cuts at:    374     468    1276    1630     374 
   Size:         94     808     354    2886

  Fragments arranged by size: 

                2886     808     354      94
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1725    1858    2317    2526    1725 
   Size:        133     459     209    3341

  Fragments arranged by size: 

                3341     459     209     133
 
TatI w'GTAC_w

Cuts at:   3657    3657 
   Size:       4142
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3659    3691    3659 
   Size:         32    4110
 
Tth111I GACn'n_nGTC

Cuts at:   1939    1939 
   Size:       4142
 
XbaI T'CTAG_A

Cuts at:   1173    1173 
   Size:       4142
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    453    1637     453 
   Size:       1184    2958
 
XmaI C'CCGG_G

Cuts at:   1162    1162 
   Size:       4142
 
XmnI GAAnn'nnTTC

Cuts at:    880     880 
   Size:       4142
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI    BmgBI     BmtI    BsaXI
    BseRI    BsiWI     BsmI    BsrGI   BssHII   BstEII  BstZ17I     ClaI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PfoI     PmlI    PshAI     PsiI
    PspXI     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com