(Circular) (Six-base) MAPSORT of: pF1KE0672.seq Check: 8961 from: 1 to: 4142 pF1KE0672 4142 bp With 182 enzymes: SgfI * April 24, 2009 13:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 895 895 Size: 4142 AccI GT'mk_AC Cuts at: 149 1180 149 Size: 1031 3111 Acc65I G'GTAC_C Cuts at: 731 1158 731 Size: 427 3715 AclI AA'CG_TT Cuts at: 3982 3982 Size: 4142 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 139 753 1967 2399 3146 3414 139 Size: 614 1214 432 747 268 867 Fragments arranged by size: 1214 867 747 614 432 268 AflIII A'CryG_T Cuts at: 1494 2598 3575 1494 Size: 1104 977 2061 Fragments arranged by size: 2061 1104 977 AgeI A'CCGG_T Cuts at: 2530 2530 Size: 4142 AlwNI CAG_nnn'CTG Cuts at: 780 1447 3014 780 Size: 667 1567 1908 Fragments arranged by size: 1908 1567 667 ApaI G_GGCC'C Cuts at: 699 699 Size: 4142 ApaLI G'TGCA_C Cuts at: 505 2912 505 Size: 2407 1735 ApoI r'AATT_y Cuts at: 1146 3495 3751 1146 Size: 2349 256 1537 Fragments arranged by size: 2349 1537 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4142 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4142 AvaI C'yCGr_G Cuts at: 174 1162 174 Size: 988 3154 BamHI G'GATC_C Cuts at: 1167 1167 Size: 4142 BanI G'GyrC_C Cuts at: 178 472 616 731 851 905 1158 1572 1820 Size: 294 144 115 120 54 253 414 248 Cuts at: 1820 1855 178 Size: 35 2465 Fragments arranged by size: 2465 414 294 253 248 144 120 115 54 35 BanII G_rGCy'C Cuts at: 217 355 656 699 767 1156 2186 217 Size: 138 301 43 68 389 1030 2173 Fragments arranged by size: 2173 1030 389 301 138 68 43 BbeI G_GCGC'C Cuts at: 476 1576 1824 476 Size: 1100 248 2794 Fragments arranged by size: 2794 1100 248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 4108 BciVI GTATCCnnnnn_n' Cuts at: 2040 2801 4142 2040 Size: 761 1341 2040 Fragments arranged by size: 2040 1341 761 BclI T'GATC_A Cuts at: 1662 1662 Size: 4142 BglI GCCn_nnn'nGGC Cuts at: 1106 1106 Size: 4142 BglII A'GATC_T Cuts at: 1657 3694 1657 Size: 2037 2105 BlpI GC'TnA_GC Cuts at: 1254 1254 Size: 4142 Bme1580I G_kGCm'C Cuts at: 509 699 1767 1860 2916 509 Size: 190 1068 93 1056 1735 Fragments arranged by size: 1735 1068 1056 190 93 BmrI ACTGGGnnnn_n' Cuts at: 1354 1435 1770 3914 1354 Size: 81 335 2144 1582 Fragments arranged by size: 2144 1582 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 231 441 819 1089 2509 231 Size: 210 378 270 1420 1864 Fragments arranged by size: 1864 1420 378 270 210 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 355 880 355 Size: 525 3617 Bpu10I CC'TnA_GC Cuts at: 761 761 Size: 4142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 220 502 1318 2197 2689 2987 220 Size: 282 816 879 492 298 1375 Fragments arranged by size: 1375 879 816 492 298 282 BsaI GGTCTCn'nnnn_ Cuts at: 576 860 3846 576 Size: 284 2986 872 Fragments arranged by size: 2986 872 284 BsaAI yAC'GTr Cuts at: 1495 1495 Size: 4142 BsaBI GATnn'nnATC Cuts at: 6 1656 3693 6 Size: 1650 2037 455 Fragments arranged by size: 2037 1650 455 BsaHI Gr'CG_yC Cuts at: 473 704 1573 1821 2523 4019 473 Size: 231 869 248 702 1496 596 Fragments arranged by size: 1496 869 702 596 248 231 BsaWI w'CCGG_w Cuts at: 971 1037 1331 1852 2530 2804 2951 3362 971 Size: 66 294 521 678 274 147 411 1751 Fragments arranged by size: 1751 678 521 411 294 274 147 66 BseYI C'CCAG_C Cuts at: 601 1044 1567 2325 2902 3398 601 Size: 443 523 758 577 496 1345 Fragments arranged by size: 1345 758 577 523 496 443 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 472 472 Size: 4142 BsiEI CG_ry'CG Cuts at: 104 1684 1730 2938 104 Size: 1580 46 1208 1308 Fragments arranged by size: 1580 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 302 509 656 767 1156 1934 2124 2916 302 Size: 207 147 111 389 778 190 792 1528 Fragments arranged by size: 1528 792 778 389 207 190 147 111 BsmBI CGTCTCn'nnnn_ Cuts at: 483 483 Size: 4142 Bsp1286I G_dGCh'C Cuts at: 217 302 355 509 656 699 767 1156 1767 Size: 85 53 154 147 43 68 389 611 Cuts at: 1767 1860 1934 2124 2186 2916 217 Size: 93 74 190 62 730 1443 Fragments arranged by size: 1443 730 611 389 190 154 147 93 85 74 68 62 53 43 BspEI T'CCGG_A Cuts at: 971 1037 3362 971 Size: 66 2325 1751 Fragments arranged by size: 2325 1751 66 BspHI T'CATG_A Cuts at: 3318 3318 Size: 4142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 494 895 1192 1406 1708 2089 494 Size: 401 297 214 302 381 2547 Fragments arranged by size: 2547 401 381 302 297 214 BsrBI CCG'CTC Cuts at: 50 210 2434 2488 3972 4137 50 Size: 160 2224 54 1484 165 55 Fragments arranged by size: 2224 1484 165 160 55 54 BsrDI GCAATG_nn' Cuts at: 2054 2054 Size: 4142 BsrFI r'CCGG_y Cuts at: 2140 2321 2530 2140 Size: 181 209 3752 Fragments arranged by size: 3752 209 181 BssSI C'ACGA_G Cuts at: 226 346 2413 2771 226 Size: 120 2067 358 1597 Fragments arranged by size: 2067 1597 358 120 BstAPI GCAn_nnn'nTGC Cuts at: 1519 1519 Size: 4142 BstBI TT'CG_AA Cuts at: 2503 2503 Size: 4142 BstXI CCAn_nnnn'nTGG Cuts at: 381 453 381 Size: 72 4070 BstYI r'GATC_y Cuts at: 1 155 776 914 968 1052 1167 1634 1657 Size: 154 621 138 54 84 115 467 23 Cuts at: 1657 1992 2238 3250 3336 3348 3694 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 621 467 449 346 335 246 154 138 115 86 84 54 23 12 Bsu36I CC'TnA_GG Cuts at: 781 781 Size: 4142 BtgI C'CryG_G Cuts at: 374 374 Size: 4142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2092 2273 2436 2092 Size: 181 163 3798 Fragments arranged by size: 3798 181 163 BtsI GCAGTG_nn' Cuts at: 302 1385 302 Size: 1083 3059 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 531 566 531 Size: 35 4107 DraI TTT'AAA Cuts at: 1141 1141 Size: 4142 DraIII CAC_nnn'GTG Cuts at: 228 228 Size: 4142 DrdI GACnn_nn'nnGTC Cuts at: 1848 2706 1848 Size: 858 3284 EaeI y'GGCC_r Cuts at: 371 1119 1727 1901 2292 2319 3664 371 Size: 748 608 174 391 27 1345 849 Fragments arranged by size: 1345 849 748 608 391 174 27 EagI C'GGCC_G Cuts at: 1727 1727 Size: 4142 EarI CTCTTCn'nnn_ Cuts at: 772 871 2165 2375 772 Size: 99 1294 210 2539 Fragments arranged by size: 2539 1294 210 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2350 2660 2806 3952 2350 Size: 310 146 1146 2540 Fragments arranged by size: 2540 1146 310 146 EcoICRI GAG'CTC Cuts at: 654 765 1154 654 Size: 111 389 3642 Fragments arranged by size: 3642 389 111 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 139 355 753 880 1967 2399 3146 3414 139 Size: 216 398 127 1087 432 747 268 867 Fragments arranged by size: 1087 867 747 432 398 268 216 127 EcoNI CCTnn'n_nnAGG Cuts at: 936 936 Size: 4142 EcoO109I rG'GnC_Cy Cuts at: 255 273 465 513 629 696 1070 1281 255 Size: 18 192 48 116 67 374 211 3116 Fragments arranged by size: 3116 374 211 192 116 67 48 18 EcoRI G'AATT_C Cuts at: 1146 1146 Size: 4142 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1023 2161 1023 Size: 1138 3004 FspI TGC'GCA Cuts at: 1923 1923 Size: 4142 HaeII r_GCGC'y Cuts at: 476 1576 1824 2846 3825 476 Size: 1100 248 1022 979 793 Fragments arranged by size: 1100 1022 979 793 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 808 840 1984 2014 2016 2046 808 Size: 32 1144 30 2 30 2904 Fragments arranged by size: 2904 1144 32 30 30 2 HincII GTy'rAC Cuts at: 1181 1181 Size: 4142 KasI G'GCGC_C Cuts at: 472 1572 1820 472 Size: 1100 248 2794 Fragments arranged by size: 2794 1100 248 KpnI G_GTAC'C Cuts at: 735 1162 735 Size: 427 3715 MluI A'CGCG_T Cuts at: 3575 3575 Size: 4142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2813 2997 2813 Size: 184 3958 MscI TGG'CCA Cuts at: 373 1121 1903 373 Size: 748 782 2612 Fragments arranged by size: 2612 782 748 MslI CAynn'nnrTG Cuts at: 2131 2258 2131 Size: 127 4015 MspA1I CmG'CkG Cuts at: 1253 1567 1927 2940 3185 1253 Size: 314 360 1013 245 2210 Fragments arranged by size: 2210 1013 360 314 245 NaeI GCC'GGC Cuts at: 2323 2323 Size: 4142 NarI GG'CG_CC Cuts at: 473 1573 1821 473 Size: 1100 248 2794 Fragments arranged by size: 2794 1100 248 NcoI C'CATG_G Cuts at: 374 374 Size: 4142 NgoMIV G'CCGG_C Cuts at: 2321 2321 Size: 4142 NspI r_CATG'y Cuts at: 612 1195 1695 2602 612 Size: 583 500 907 2152 Fragments arranged by size: 2152 907 583 500 PasI CC'CwG_GG Cuts at: 699 699 Size: 4142 PciI A'CATG_T Cuts at: 2598 2598 Size: 4142 PflMI CCAn_nnn'nTGG Cuts at: 456 456 Size: 4142 PmeI GTTT'AAAC Cuts at: 1141 1141 Size: 4142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3281 3313 3281 Size: 32 4110 PpuMI rG'GwC_Cy Cuts at: 465 513 629 465 Size: 48 116 3978 Fragments arranged by size: 3978 116 48 PspOMI G'GGCC_C Cuts at: 695 695 Size: 4142 PstI C_TGCA'G Cuts at: 1189 1874 1189 Size: 685 3457 PvuI CG_AT'CG Cuts at: 104 104 Size: 4142 PvuII CAG'CTG Cuts at: 1567 1927 1567 Size: 360 3782 RsrII CG'GwC_CG Cuts at: 2337 2337 Size: 4142 SacI G_AGCT'C Cuts at: 656 767 1156 656 Size: 111 389 3642 Fragments arranged by size: 3642 389 111 SalI G'TCGA_C Cuts at: 1179 1179 Size: 4142 SanDI GG'GwC_CC Cuts at: 629 629 Size: 4142 SapI GCTCTTCn'nnn_ Cuts at: 772 2165 2375 772 Size: 1393 210 2539 Fragments arranged by size: 2539 1393 210 SbfI CC_TGCA'GG Cuts at: 1189 1189 Size: 4142 ScaI AGT'ACT Cuts at: 3659 3659 Size: 4142 SfcI C'TryA_G Cuts at: 33 1185 1870 2863 3054 33 Size: 1152 685 993 191 1121 Fragments arranged by size: 1152 1121 993 685 191 SfoI GGC'GCC Cuts at: 474 1574 1822 474 Size: 1100 248 2794 Fragments arranged by size: 2794 1100 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4142 SmaI CCC'GGG Cuts at: 1164 1164 Size: 4142 SmlI C'TyrA_G Cuts at: 235 517 1297 2212 2704 2966 235 Size: 282 780 915 492 262 1411 Fragments arranged by size: 1411 915 780 492 282 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4142 SphI G_CATG'C Cuts at: 1195 1695 1195 Size: 500 3642 StuI AGG'CCT Cuts at: 898 1109 898 Size: 211 3931 StyI C'CwwG_G Cuts at: 374 468 1276 1630 374 Size: 94 808 354 2886 Fragments arranged by size: 2886 808 354 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1725 1858 2317 2526 1725 Size: 133 459 209 3341 Fragments arranged by size: 3341 459 209 133 TatI w'GTAC_w Cuts at: 3657 3657 Size: 4142 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3659 3691 3659 Size: 32 4110 Tth111I GACn'n_nGTC Cuts at: 1939 1939 Size: 4142 XbaI T'CTAG_A Cuts at: 1173 1173 Size: 4142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 453 1637 453 Size: 1184 2958 XmaI C'CCGG_G Cuts at: 1162 1162 Size: 4142 XmnI GAAnn'nnTTC Cuts at: 880 880 Size: 4142 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI BseRI BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI