Restriction map for
pF1KE0668
(Circular) (Six-base) MAPSORT of: pF1KE0668.seq Check: 4306 from: 1 to: 4292
pF1KE0668 4292 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1330 1330
Size: 4292
Acc65I G'GTAC_C
Cuts at: 1308 1308
Size: 4292
AclI AA'CG_TT
Cuts at: 4132 4132
Size: 4292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 592 2117 2549 3296 3564 316
Size: 276 1525 432 747 268 1044
Fragments arranged by size:
1525 1044 747 432 276 268
AflIII A'CryG_T
Cuts at: 794 1644 2748 3725 794
Size: 850 1104 977 1361
Fragments arranged by size:
1361 1104 977 850
AgeI A'CCGG_T
Cuts at: 2680 2680
Size: 4292
AlwNI CAG_nnn'CTG
Cuts at: 459 530 1597 3164 459
Size: 71 1067 1567 1587
Fragments arranged by size:
1587 1567 1067 71
ApaLI G'TGCA_C
Cuts at: 3062 3062
Size: 4292
ApoI r'AATT_y
Cuts at: 587 599 1105 1296 3645 3901 587
Size: 12 506 191 2349 256 978
Fragments arranged by size:
2349 978 506 256 191 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 4292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
AvaI C'yCGr_G
Cuts at: 985 1312 985
Size: 327 3965
BamHI G'GATC_C
Cuts at: 1317 1317
Size: 4292
BanI G'GyrC_C
Cuts at: 935 1308 1722 1970 2005 935
Size: 373 414 248 35 3222
Fragments arranged by size:
3222 414 373 248 35
BanII G_rGCy'C
Cuts at: 1306 2336 1306
Size: 1030 3262
BbeI G_GCGC'C
Cuts at: 1726 1974 1726
Size: 248 4044
BbvCI CC'TCA_GC
Cuts at: 1205 1205
Size: 4292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 142 176 90
Size: 34 18 34 4206
Fragments arranged by size:
4206 34 34 18
BciVI GTATCCnnnnn_n'
Cuts at: 899 2190 2951 4292 899
Size: 1291 761 1341 899
Fragments arranged by size:
1341 1291 899 761
BclI T'GATC_A
Cuts at: 1812 1812
Size: 4292
BglII A'GATC_T
Cuts at: 1195 1807 3844 1195
Size: 612 2037 1643
Fragments arranged by size:
2037 1643 612
BlpI GC'TnA_GC
Cuts at: 1404 1404
Size: 4292
Bme1580I G_kGCm'C
Cuts at: 784 1917 2010 3066 784
Size: 1133 93 1056 2010
Fragments arranged by size:
2010 1133 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1079 1504 1585 1920 4064 1079
Size: 425 81 335 2144 1307
Fragments arranged by size:
2144 1307 425 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 489 2659 489
Size: 2170 2122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 430 796 867 887 940 238
Size: 192 366 71 20 53 3590
Fragments arranged by size:
3590 366 192 71 53 20
Bpu10I CC'TnA_GC
Cuts at: 997 1205 997
Size: 208 4084
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1468 2347 2839 3137 277
Size: 1191 879 492 298 1432
Fragments arranged by size:
1432 1191 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 181 366 955 3996 181
Size: 185 589 3041 477
Fragments arranged by size:
3041 589 477 185
BsaAI yAC'GTr
Cuts at: 1645 1645
Size: 4292
BsaBI GATnn'nnATC
Cuts at: 6 526 1806 3843 6
Size: 520 1280 2037 455
Fragments arranged by size:
2037 1280 520 455
BsaHI Gr'CG_yC
Cuts at: 127 1283 1723 1971 2673 4169 127
Size: 1156 440 248 702 1496 250
Fragments arranged by size:
1496 1156 702 440 250 248
BsaWI w'CCGG_w
Cuts at: 428 1481 2002 2680 2954 3101 3512 428
Size: 1053 521 678 274 147 411 1208
Fragments arranged by size:
1208 1053 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 913 943 913
Size: 30 4262
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 890 1061 235
Size: 655 171 3466
Fragments arranged by size:
3466 655 171
BseYI C'CCAG_C
Cuts at: 327 1258 1717 2475 3052 3548 327
Size: 931 459 758 577 496 1071
Fragments arranged by size:
1071 931 758 577 496 459
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 853
Size: 4292
BsiEI CG_ry'CG
Cuts at: 104 1834 1880 3088 104
Size: 1730 46 1208 1308
Fragments arranged by size:
1730 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 542 1306 2084 2274 3066 542
Size: 764 778 190 792 1768
Fragments arranged by size:
1768 792 778 764 190
Bsp1286I G_dGCh'C
Cuts at: 542 784 1306 1917 2010 2084 2274 2336 3066
Size: 242 522 611 93 74 190 62 730
Cuts at: 3066 542
Size: 1768
Fragments arranged by size:
1768 730 611 522 242 190 93 74
62
BspEI T'CCGG_A
Cuts at: 428 3512 428
Size: 3084 1208
BspHI T'CATG_A
Cuts at: 3468 3468
Size: 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 444 1213 1342 1556 1858 2239 444
Size: 769 129 214 302 381 2497
Fragments arranged by size:
2497 769 381 302 214 129
BsrBI CCG'CTC
Cuts at: 50 2584 2638 4122 4287 50
Size: 2534 54 1484 165 55
Fragments arranged by size:
2534 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 345 617 2204 345
Size: 272 1587 2433
Fragments arranged by size:
2433 1587 272
BsrFI r'CCGG_y
Cuts at: 2290 2471 2680 2290
Size: 181 209 3902
Fragments arranged by size:
3902 209 181
BssSI C'ACGA_G
Cuts at: 2563 2921 2563
Size: 358 3934
BstAPI GCAn_nnn'nTGC
Cuts at: 459 1669 459
Size: 1210 3082
BstBI TT'CG_AA
Cuts at: 2653 2653
Size: 4292
BstXI CCAn_nnnn'nTGG
Cuts at: 918 1279 918
Size: 361 3931
BstYI r'GATC_y
Cuts at: 1 1195 1317 1784 1807 2142 2388 3400 3486
Size: 1194 122 467 23 335 246 1012 86
Cuts at: 3486 3498 3844 1
Size: 12 346 449
Fragments arranged by size:
1194 1012 467 449 346 335 246 122
86 23 12
BtgI C'CryG_G
Cuts at: 708 761 1028 708
Size: 53 267 3972
Fragments arranged by size:
3972 267 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 527 2242 2423 2586 527
Size: 1715 181 163 2233
Fragments arranged by size:
2233 1715 181 163
BtsI GCAGTG_nn'
Cuts at: 1535 1535
Size: 4292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 690 725 690
Size: 35 4257
DraI TTT'AAA
Cuts at: 1291 1291
Size: 4292
DrdI GACnn_nn'nnGTC
Cuts at: 736 1998 2856 736
Size: 1262 858 2172
Fragments arranged by size:
2172 1262 858
EaeI y'GGCC_r
Cuts at: 389 764 1877 2051 2442 2469 3814 389
Size: 375 1113 174 391 27 1345 867
Fragments arranged by size:
1345 1113 867 391 375 174 27
EagI C'GGCC_G
Cuts at: 1877 1877
Size: 4292
EarI CTCTTCn'nnn_
Cuts at: 2315 2525 2315
Size: 210 4082
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2500 2810 2956 4102 2500
Size: 310 146 1146 2690
Fragments arranged by size:
2690 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1304 1304
Size: 4292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 316 430 592 796 867 887 940 2117
Size: 78 114 162 204 71 20 53 1177
Cuts at: 2117 2549 3296 3564 238
Size: 432 747 268 966
Fragments arranged by size:
1177 966 747 432 268 204 162 114
78 71 53 20
EcoO109I rG'GnC_Cy
Cuts at: 788 980 992 1431 788
Size: 192 12 439 3649
Fragments arranged by size:
3649 439 192 12
EcoRI G'AATT_C
Cuts at: 587 1296 587
Size: 709 3583
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 4292
FspI TGC'GCA
Cuts at: 2073 2073
Size: 4292
HaeII r_GCGC'y
Cuts at: 255 1726 1974 2996 3975 255
Size: 1471 248 1022 979 572
Fragments arranged by size:
1471 1022 979 572 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2134 2164 2166 2196 2134
Size: 30 2 30 4230
Fragments arranged by size:
4230 30 30 2
HincII GTy'rAC
Cuts at: 168 702 1062 1331 168
Size: 534 360 269 3129
Fragments arranged by size:
3129 534 360 269
HpaI GTT'AAC
Cuts at: 168 168
Size: 4292
KasI G'GCGC_C
Cuts at: 1722 1970 1722
Size: 248 4044
KpnI G_GTAC'C
Cuts at: 1312 1312
Size: 4292
MluI A'CGCG_T
Cuts at: 3725 3725
Size: 4292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 713 1028 2963 3147 713
Size: 315 1935 184 1858
Fragments arranged by size:
1935 1858 315 184
MscI TGG'CCA
Cuts at: 391 766 2053 391
Size: 375 1287 2630
Fragments arranged by size:
2630 1287 375
MslI CAynn'nnrTG
Cuts at: 545 674 2281 2408 545
Size: 129 1607 127 2429
Fragments arranged by size:
2429 1607 129 127
MspA1I CmG'CkG
Cuts at: 138 743 1403 1717 2077 3090 3335 138
Size: 605 660 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 660 605 360 314 245
NaeI GCC'GGC
Cuts at: 2473 2473
Size: 4292
NarI GG'CG_CC
Cuts at: 1723 1971 1723
Size: 248 4044
NcoI C'CATG_G
Cuts at: 761 1028 761
Size: 267 4025
NgoMIV G'CCGG_C
Cuts at: 2471 2471
Size: 4292
NspI r_CATG'y
Cuts at: 798 1345 1845 2752 798
Size: 547 500 907 2338
Fragments arranged by size:
2338 907 547 500
PciI A'CATG_T
Cuts at: 794 2748 794
Size: 1954 2338
PmeI GTTT'AAAC
Cuts at: 1291 1291
Size: 4292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 3431
Size: 32 4260
PpuMI rG'GwC_Cy
Cuts at: 788 980 788
Size: 192 4100
PstI C_TGCA'G
Cuts at: 165 455 1339 2024 165
Size: 290 884 685 2433
Fragments arranged by size:
2433 884 685 290
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4292
PvuII CAG'CTG
Cuts at: 138 1717 2077 138
Size: 1579 360 2353
Fragments arranged by size:
2353 1579 360
RsrII CG'GwC_CG
Cuts at: 2487 2487
Size: 4292
SacI G_AGCT'C
Cuts at: 1306 1306
Size: 4292
SalI G'TCGA_C
Cuts at: 1329 1329
Size: 4292
SapI GCTCTTCn'nnn_
Cuts at: 2315 2525 2315
Size: 210 4082
SbfI CC_TGCA'GG
Cuts at: 1339 1339
Size: 4292
ScaI AGT'ACT
Cuts at: 3809 3809
Size: 4292
SfcI C'TryA_G
Cuts at: 33 161 451 507 1335 2020 3013 3204 33
Size: 128 290 56 828 685 993 191 1121
Fragments arranged by size:
1121 993 828 685 290 191 128 56
SfoI GGC'GCC
Cuts at: 1724 1972 1724
Size: 248 4044
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
SmaI CCC'GGG
Cuts at: 987 1314 987
Size: 327 3965
SmlI C'TyrA_G
Cuts at: 256 1447 2362 2854 3116 256
Size: 1191 915 492 262 1432
Fragments arranged by size:
1432 1191 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4292
SphI G_CATG'C
Cuts at: 1345 1845 1345
Size: 500 3792
StuI AGG'CCT
Cuts at: 202 262 635 202
Size: 60 373 3859
Fragments arranged by size:
3859 373 60
StyI C'CwwG_G
Cuts at: 761 911 976 1028 1426 1780 761
Size: 150 65 52 398 354 3273
Fragments arranged by size:
3273 398 354 150 65 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 923 984 1875 2008 2467 2676 923
Size: 61 891 133 459 209 2539
Fragments arranged by size:
2539 891 459 209 133 61
TatI w'GTAC_w
Cuts at: 3807 3807
Size: 4292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3809 3841 3809
Size: 32 4260
Tth111I GACn'n_nGTC
Cuts at: 2089 2089
Size: 4292
XbaI T'CTAG_A
Cuts at: 1323 1323
Size: 4292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 373 774 1787 373
Size: 401 1013 2878
Fragments arranged by size:
2878 1013 401
XmaI C'CCGG_G
Cuts at: 985 1312 985
Size: 327 3965
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI