Restriction map for
pF1KE0667
(Circular) (Six-base) MAPSORT of: pF1KE0667.seq Check: 7308 from: 1 to: 3842
pF1KE0667 3842 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 836 3682 836
Size: 2846 996
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 402 1667 2099 2846 3114 188
Size: 214 1265 432 747 268 916
Fragments arranged by size:
1265 916 747 432 268 214
AflIII A'CryG_T
Cuts at: 159 402 734 1194 2298 3275 159
Size: 243 332 460 1104 977 726
Fragments arranged by size:
1104 977 726 460 332 243
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AlwNI CAG_nnn'CTG
Cuts at: 209 706 1147 2714 209
Size: 497 441 1567 1337
Fragments arranged by size:
1567 1337 497 441
ApaLI G'TGCA_C
Cuts at: 730 2612 730
Size: 1882 1960
ApoI r'AATT_y
Cuts at: 337 846 3195 3451 337
Size: 509 2349 256 728
Fragments arranged by size:
2349 728 509 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 440 862 440
Size: 422 3420
BamHI G'GATC_C
Cuts at: 867 867
Size: 3842
BanI G'GyrC_C
Cuts at: 858 1272 1520 1555 858
Size: 414 248 35 3145
Fragments arranged by size:
3145 414 248 35
BanII G_rGCy'C
Cuts at: 145 695 856 1886 145
Size: 550 161 1030 2101
Fragments arranged by size:
2101 1030 550 161
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2501 3842 1740
Size: 761 1341 1740
Fragments arranged by size:
1740 1341 761
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 954 954
Size: 3842
Bme1580I G_kGCm'C
Cuts at: 734 1467 1560 2616 734
Size: 733 93 1056 1960
Fragments arranged by size:
1960 1056 733 93
BmgBI CAC'GTC
Cuts at: 162 403 162
Size: 241 3601
BmrI ACTGGGnnnn_n'
Cuts at: 219 1054 1135 1470 3614 219
Size: 835 81 335 2144 447
Fragments arranged by size:
2144 835 447 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 3842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 500
Size: 3842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1897 2389 2687 1018
Size: 879 492 298 2173
Fragments arranged by size:
2173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3546 3546
Size: 3842
BsaAI yAC'GTr
Cuts at: 735 1195 735
Size: 460 3382
BsaBI GATnn'nnATC
Cuts at: 6 1356 3393 6
Size: 1350 2037 455
Fragments arranged by size:
2037 1350 455
BsaHI Gr'CG_yC
Cuts at: 807 1273 1521 2223 3719 807
Size: 466 248 702 1496 930
Fragments arranged by size:
1496 930 702 466 248
BsaWI w'CCGG_w
Cuts at: 1031 1552 2230 2504 2651 3062 1031
Size: 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 153 640 153
Size: 487 3355
BseYI C'CCAG_C
Cuts at: 554 1267 2025 2602 3098 554
Size: 713 758 577 496 1298
Fragments arranged by size:
1298 758 713 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 523
Size: 3842
BsiEI CG_ry'CG
Cuts at: 104 1384 1430 2638 104
Size: 1280 46 1208 1308
Fragments arranged by size:
1308 1280 1208 46
BsiHKAI G_wGCw'C
Cuts at: 695 734 856 1634 1824 2616 695
Size: 39 122 778 190 792 1921
Fragments arranged by size:
1921 792 778 190 122 39
Bsp1286I G_dGCh'C
Cuts at: 145 695 734 856 1467 1560 1634 1824 1886
Size: 550 39 122 611 93 74 190 62
Cuts at: 1886 2616 145
Size: 730 1371
Fragments arranged by size:
1371 730 611 550 190 122 93 74
62 39
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 459 3018 459
Size: 2559 1283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1106 1408 1789 892
Size: 214 302 381 2945
Fragments arranged by size:
2945 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2134 2188 3672 3837 50
Size: 2084 54 1484 165 55
Fragments arranged by size:
2084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 300 1754 300
Size: 1454 2388
BsrFI r'CCGG_y
Cuts at: 1840 2021 2230 1840
Size: 181 209 3452
Fragments arranged by size:
3452 209 181
BssSI C'ACGA_G
Cuts at: 2113 2471 2113
Size: 358 3484
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 1219
Size: 3842
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstYI r'GATC_y
Cuts at: 1 867 1334 1357 1692 1938 2950 3036 3048
Size: 866 467 23 335 246 1012 86 12
Cuts at: 3048 3394 1
Size: 346 449
Fragments arranged by size:
1012 866 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1792 1973 2136 1792
Size: 181 163 3498
Fragments arranged by size:
3498 181 163
BtsI GCAGTG_nn'
Cuts at: 1085 1085
Size: 3842
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 214 1548 2406 214
Size: 1334 858 1650
Fragments arranged by size:
1650 1334 858
EaeI y'GGCC_r
Cuts at: 662 1427 1601 1992 2019 3364 662
Size: 765 174 391 27 1345 1140
Fragments arranged by size:
1345 1140 765 391 174 27
EagI C'GGCC_G
Cuts at: 1427 1427
Size: 3842
EarI CTCTTCn'nnn_
Cuts at: 317 755 1865 2075 317
Size: 438 1110 210 2084
Fragments arranged by size:
2084 1110 438 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2050 2360 2506 3652 2050
Size: 310 146 1146 2240
Fragments arranged by size:
2240 1146 310 146
EcoICRI GAG'CTC
Cuts at: 693 854 693
Size: 161 3681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 402 500 1667 2099 2846 3114 188
Size: 214 98 1167 432 747 268 916
Fragments arranged by size:
1167 916 747 432 268 214 98
EcoO109I rG'GnC_Cy
Cuts at: 765 981 765
Size: 216 3626
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 513 1861 513
Size: 1348 2494
FspI TGC'GCA
Cuts at: 1623 1623
Size: 3842
HaeII r_GCGC'y
Cuts at: 1276 1524 2546 3525 1276
Size: 248 1022 979 1593
Fragments arranged by size:
1593 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1684 1714 1716 1746 1684
Size: 30 2 30 3780
Fragments arranged by size:
3780 30 30 2
HincII GTy'rAC
Cuts at: 881 881
Size: 3842
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 857 2513 2697 857
Size: 1656 184 2002
Fragments arranged by size:
2002 1656 184
MscI TGG'CCA
Cuts at: 664 1603 664
Size: 939 2903
MslI CAynn'nnrTG
Cuts at: 133 1831 1958 133
Size: 1698 127 2017
Fragments arranged by size:
2017 1698 127
MspA1I CmG'CkG
Cuts at: 953 1267 1627 2640 2885 953
Size: 314 360 1013 245 1910
Fragments arranged by size:
1910 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 3842
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 3842
NspI r_CATG'y
Cuts at: 895 1395 2302 895
Size: 500 907 2435
Fragments arranged by size:
2435 907 500
PasI CC'CwG_GG
Cuts at: 145 145
Size: 3842
PciI A'CATG_T
Cuts at: 2298 2298
Size: 3842
PflMI CCAn_nnn'nTGG
Cuts at: 601 601
Size: 3842
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PmlI CAC'GTG
Cuts at: 735 735
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 3810
PpuMI rG'GwC_Cy
Cuts at: 765 765
Size: 3842
PstI C_TGCA'G
Cuts at: 438 889 1574 438
Size: 451 685 2706
Fragments arranged by size:
2706 685 451
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 1267 1627 1267
Size: 360 3482
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 695 856 695
Size: 161 3681
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SfcI C'TryA_G
Cuts at: 33 434 885 1570 2563 2754 33
Size: 401 451 685 993 191 1121
Fragments arranged by size:
1121 993 685 451 401 191
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 997 1912 2404 2666 997
Size: 915 492 262 2173
Fragments arranged by size:
2173 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StyI C'CwwG_G
Cuts at: 976 1330 976
Size: 354 3488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1425 1558 2017 2226 1425
Size: 133 459 209 3041
Fragments arranged by size:
3041 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 3810
Tth111I GACn'n_nGTC
Cuts at: 708 1639 708
Size: 931 2911
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 602 1337 602
Size: 735 3107
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
XmnI GAAnn'nnTTC
Cuts at: 242 242
Size: 3842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI