Restriction map for
pF1KE0666
(Circular) (Six-base) MAPSORT of: pF1KE0666.seq Check: 9017 from: 1 to: 4184
pF1KE0666 4184 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2009 2441 3188 3456 2009
Size: 432 747 268 2737
Fragments arranged by size:
2737 747 432 268
AfeI AGC'GCT
Cuts at: 241 241
Size: 4184
AflIII A'CryG_T
Cuts at: 1536 2640 3617 1536
Size: 1104 977 2103
Fragments arranged by size:
2103 1104 977
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AhdI GACnn_n'nnGTC
Cuts at: 680 680
Size: 4184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 169 201 169
Size: 32 4152
AlwNI CAG_nnn'CTG
Cuts at: 1489 3056 1489
Size: 1567 2617
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 814 1188 3537 3793 814
Size: 374 2349 256 1205
Fragments arranged by size:
2349 1205 374 256
AseI AT'TA_AT
Cuts at: 20 949 20
Size: 929 3255
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 1204 1204
Size: 4184
BamHI G'GATC_C
Cuts at: 1209 1209
Size: 4184
BanI G'GyrC_C
Cuts at: 135 415 1200 1614 1862 1897 135
Size: 280 785 414 248 35 2422
Fragments arranged by size:
2422 785 414 280 248 35
BanII G_rGCy'C
Cuts at: 323 1198 2228 323
Size: 875 1030 2279
Fragments arranged by size:
2279 1030 875
BbeI G_GCGC'C
Cuts at: 1618 1866 1618
Size: 248 3936
BbsI GAAGACnn'nnnn_
Cuts at: 232 324 232
Size: 92 4092
BciVI GTATCCnnnnn_n'
Cuts at: 2082 2843 4184 2082
Size: 761 1341 2082
Fragments arranged by size:
2082 1341 761
BclI T'GATC_A
Cuts at: 1704 1704
Size: 4184
BfrBI ATG'CAT
Cuts at: 826 826
Size: 4184
BglII A'GATC_T
Cuts at: 966 1699 3736 966
Size: 733 2037 1414
Fragments arranged by size:
2037 1414 733
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 1809 1902 2958 1809
Size: 93 1056 3035
Fragments arranged by size:
3035 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1396 1477 1812 3956 1396
Size: 81 335 2144 1624
Fragments arranged by size:
2144 1624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 452 2551 452
Size: 2099 2085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 476 931 140
Size: 336 455 3393
Fragments arranged by size:
3393 455 336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 409 1360 2239 2731 3029 196
Size: 213 951 879 492 298 1351
Fragments arranged by size:
1351 951 879 492 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 3888 3888
Size: 4184
BsaAI yAC'GTr
Cuts at: 1537 1537
Size: 4184
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 978 1615 1863 2565 4061 978
Size: 637 248 702 1496 1101
Fragments arranged by size:
1496 1101 702 637 248
BsaWI w'CCGG_w
Cuts at: 1373 1894 2572 2846 2993 3404 1373
Size: 521 678 274 147 411 2153
Fragments arranged by size:
2153 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 199 169
Size: 30 4154
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 298 1075 190
Size: 108 777 3299
Fragments arranged by size:
3299 777 108
BseYI C'CCAG_C
Cuts at: 322 1609 2367 2944 3440 322
Size: 1287 758 577 496 1066
Fragments arranged by size:
1287 1066 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 608
Size: 4184
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1198 1976 2166 2958 1198
Size: 778 190 792 2424
Fragments arranged by size:
2424 792 778 190
BsmI GAATG_Cn'
Cuts at: 658 658
Size: 4184
Bsp1286I G_dGCh'C
Cuts at: 323 1198 1809 1902 1976 2166 2228 2958 323
Size: 875 611 93 74 190 62 730 1549
Fragments arranged by size:
1549 875 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 3360 3360
Size: 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 864 1234 1448 1750 2131 864
Size: 370 214 302 381 2917
Fragments arranged by size:
2917 381 370 302 214
BsrBI CCG'CTC
Cuts at: 50 2476 2530 4014 4179 50
Size: 2426 54 1484 165 55
Fragments arranged by size:
2426 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2096
Size: 4184
BsrFI r'CCGG_y
Cuts at: 132 2182 2363 2572 132
Size: 2050 181 209 1744
Fragments arranged by size:
2050 1744 209 181
BssSI C'ACGA_G
Cuts at: 2455 2813 2455
Size: 358 3826
BstAPI GCAn_nnn'nTGC
Cuts at: 1561 1561
Size: 4184
BstBI TT'CG_AA
Cuts at: 305 2545 305
Size: 2240 1944
BstXI CCAn_nnnn'nTGG
Cuts at: 384 384
Size: 4184
BstYI r'GATC_y
Cuts at: 1 966 1209 1676 1699 2034 2280 3292 3378
Size: 965 243 467 23 335 246 1012 86
Cuts at: 3378 3390 3736 1
Size: 12 346 449
Fragments arranged by size:
1012 965 467 449 346 335 246 243
86 23 12
BtgI C'CryG_G
Cuts at: 107 348 701 107
Size: 241 353 3590
Fragments arranged by size:
3590 353 241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 1427 1427
Size: 4184
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DrdI GACnn_nn'nnGTC
Cuts at: 1890 2748 1890
Size: 858 3326
EaeI y'GGCC_r
Cuts at: 1769 1943 2334 2361 3706 1769
Size: 174 391 27 1345 2247
Fragments arranged by size:
2247 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 256 307 1052 2207 2417 256
Size: 51 745 1155 210 2023
Fragments arranged by size:
2023 1155 745 210 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2392 2702 2848 3994 2392
Size: 310 146 1146 2582
Fragments arranged by size:
2582 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1196 1196
Size: 4184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 476 931 2009 2441 3188 3456 140
Size: 336 455 1078 432 747 268 868
Fragments arranged by size:
1078 868 747 455 432 336 268
EcoO109I rG'GnC_Cy
Cuts at: 1323 1323
Size: 4184
EcoRI G'AATT_C
Cuts at: 1188 1188
Size: 4184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 318 2203 318
Size: 1885 2299
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 115 243 1618 1866 2888 3867 115
Size: 128 1375 248 1022 979 432
Fragments arranged by size:
1375 1022 979 432 248 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 666 698 811 843 951 983 2026 2056 2058
Size: 32 113 32 108 32 1043 30 2
Cuts at: 2058 2088 666
Size: 30 2762
Fragments arranged by size:
2762 1043 113 108 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1223 1223
Size: 4184
KasI G'GCGC_C
Cuts at: 1614 1862 1614
Size: 248 3936
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1116 2855 3039 1116
Size: 1739 184 2261
Fragments arranged by size:
2261 1739 184
MscI TGG'CCA
Cuts at: 1945 1945
Size: 4184
MslI CAynn'nnrTG
Cuts at: 831 2173 2300 831
Size: 1342 127 2715
Fragments arranged by size:
2715 1342 127
MspA1I CmG'CkG
Cuts at: 1295 1609 1969 2982 3227 1295
Size: 314 360 1013 245 2252
Fragments arranged by size:
2252 1013 360 314 245
NaeI GCC'GGC
Cuts at: 134 2365 134
Size: 2231 1953
NarI GG'CG_CC
Cuts at: 1615 1863 1615
Size: 248 3936
NcoI C'CATG_G
Cuts at: 107 701 107
Size: 594 3590
NgoMIV G'CCGG_C
Cuts at: 132 2363 132
Size: 2231 1953
NsiI A_TGCA'T
Cuts at: 828 828
Size: 4184
NspI r_CATG'y
Cuts at: 1237 1737 2644 1237
Size: 500 907 2777
Fragments arranged by size:
2777 907 500
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 169 201 3323 3355 169
Size: 32 3122 32 998
Fragments arranged by size:
3122 998 32 32
PstI C_TGCA'G
Cuts at: 1231 1916 1231
Size: 685 3499
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 1609 1969 1609
Size: 360 3824
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 1198 1198
Size: 4184
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 307 2207 2417 307
Size: 1900 210 2074
Fragments arranged by size:
2074 1900 210
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 3701 3701
Size: 4184
SfcI C'TryA_G
Cuts at: 33 584 1227 1912 2905 3096 33
Size: 551 643 685 993 191 1121
Fragments arranged by size:
1121 993 685 643 551 191
SfoI GGC'GCC
Cuts at: 1616 1864 1616
Size: 248 3936
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 211 424 1339 2254 2746 3008 211
Size: 213 915 915 492 262 1387
Fragments arranged by size:
1387 915 915 492 262 213
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1237 1737 1237
Size: 500 3684
StyI C'CwwG_G
Cuts at: 107 701 1318 1672 107
Size: 594 617 354 2619
Fragments arranged by size:
2619 617 594 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1767 1900 2359 2568 1767
Size: 133 459 209 3383
Fragments arranged by size:
3383 459 209 133
TatI w'GTAC_w
Cuts at: 3699 3699
Size: 4184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 4152
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 906 1679 906
Size: 773 3411
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI