Restriction map for
pF1KE0665
(Circular) (Six-base) MAPSORT of: pF1KE0665.seq Check: 6543 from: 1 to: 3833
pF1KE0665 3833 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 871 871
Size: 3833
Acc65I G'GTAC_C
Cuts at: 849 849
Size: 3833
AclI AA'CG_TT
Cuts at: 3673 3673
Size: 3833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 1658 2090 2837 3105 540
Size: 1118 432 747 268 1268
Fragments arranged by size:
1268 1118 747 432 268
AflIII A'CryG_T
Cuts at: 443 1185 2289 3266 443
Size: 742 1104 977 1010
Fragments arranged by size:
1104 1010 977 742
AgeI A'CCGG_T
Cuts at: 2221 2221
Size: 3833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 637 637
Size: 3833
AlwNI CAG_nnn'CTG
Cuts at: 1138 2705 1138
Size: 1567 2266
ApaLI G'TGCA_C
Cuts at: 2603 2603
Size: 3833
ApoI r'AATT_y
Cuts at: 837 3186 3442 837
Size: 2349 256 1228
Fragments arranged by size:
2349 1228 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3833
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
AvaI C'yCGr_G
Cuts at: 704 853 704
Size: 149 3684
BamHI G'GATC_C
Cuts at: 858 858
Size: 3833
BanI G'GyrC_C
Cuts at: 136 849 1263 1511 1546 136
Size: 713 414 248 35 2423
Fragments arranged by size:
2423 713 414 248 35
BanII G_rGCy'C
Cuts at: 847 1877 847
Size: 1030 2803
BbeI G_GCGC'C
Cuts at: 1267 1515 1267
Size: 248 3585
BciVI GTATCCnnnnn_n'
Cuts at: 1731 2492 3833 1731
Size: 761 1341 1731
Fragments arranged by size:
1731 1341 761
BclI T'GATC_A
Cuts at: 1353 1353
Size: 3833
BfrBI ATG'CAT
Cuts at: 624 624
Size: 3833
BglII A'GATC_T
Cuts at: 814 1348 3385 814
Size: 534 2037 1262
Fragments arranged by size:
2037 1262 534
BlpI GC'TnA_GC
Cuts at: 945 945
Size: 3833
Bme1580I G_kGCm'C
Cuts at: 1458 1551 2607 1458
Size: 93 1056 2684
Fragments arranged by size:
2684 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 279 1045 1126 1461 3605 279
Size: 766 81 335 2144 507
Fragments arranged by size:
2144 766 507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 3833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1888 2380 2678 1009
Size: 879 492 298 2164
Fragments arranged by size:
2164 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 419 722 3537 419
Size: 303 2815 715
Fragments arranged by size:
2815 715 303
BsaAI yAC'GTr
Cuts at: 1186 1186
Size: 3833
BsaBI GATnn'nnATC
Cuts at: 6 693 699 1347 3384 6
Size: 687 6 648 2037 455
Fragments arranged by size:
2037 687 648 455 6
BsaHI Gr'CG_yC
Cuts at: 1264 1512 2214 3710 1264
Size: 248 702 1496 1387
Fragments arranged by size:
1496 1387 702 248
BsaWI w'CCGG_w
Cuts at: 1022 1543 2221 2495 2642 3053 1022
Size: 521 678 274 147 411 1802
Fragments arranged by size:
1802 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 367 397 367
Size: 30 3803
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 391 412 370
Size: 21 21 3791
Fragments arranged by size:
3791 21 21
BseYI C'CCAG_C
Cuts at: 1258 2016 2593 3089 1258
Size: 758 577 496 2002
Fragments arranged by size:
2002 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 799 1375 1421 2629 104
Size: 695 576 46 1208 1308
Fragments arranged by size:
1308 1208 695 576 46
BsiHKAI G_wGCw'C
Cuts at: 208 847 1625 1815 2607 208
Size: 639 778 190 792 1434
Fragments arranged by size:
1434 792 778 639 190
BsmI GAATG_Cn'
Cuts at: 598 598
Size: 3833
Bsp1286I G_dGCh'C
Cuts at: 208 847 1458 1551 1625 1815 1877 2607 208
Size: 639 611 93 74 190 62 730 1434
Fragments arranged by size:
1434 730 639 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3053 3053
Size: 3833
BspHI T'CATG_A
Cuts at: 3009 3009
Size: 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 883 1097 1399 1780 883
Size: 214 302 381 2936
Fragments arranged by size:
2936 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2125 2179 3663 3828 50
Size: 2075 54 1484 165 55
Fragments arranged by size:
2075 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 242 1745 242
Size: 1503 2330
BsrFI r'CCGG_y
Cuts at: 1831 2012 2221 1831
Size: 181 209 3443
Fragments arranged by size:
3443 209 181
BssSI C'ACGA_G
Cuts at: 599 2104 2462 599
Size: 1505 358 1970
Fragments arranged by size:
1970 1505 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1210 1210
Size: 3833
BstBI TT'CG_AA
Cuts at: 2194 2194
Size: 3833
BstYI r'GATC_y
Cuts at: 1 694 814 858 1325 1348 1683 1929 2941
Size: 693 120 44 467 23 335 246 1012
Cuts at: 2941 3027 3039 3385 1
Size: 86 12 346 449
Fragments arranged by size:
1012 693 467 449 346 335 246 120
86 44 23 12
BtgI C'CryG_G
Cuts at: 107 732 107
Size: 625 3208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 332 1783 1964 2127 332
Size: 1451 181 163 2038
Fragments arranged by size:
2038 1451 181 163
BtsI GCAGTG_nn'
Cuts at: 1076 1076
Size: 3833
DraI TTT'AAA
Cuts at: 832 832
Size: 3833
DraIII CAC_nnn'GTG
Cuts at: 435 435
Size: 3833
DrdI GACnn_nn'nnGTC
Cuts at: 1539 2397 1539
Size: 858 2975
EaeI y'GGCC_r
Cuts at: 666 1418 1592 1983 2010 3355 666
Size: 752 174 391 27 1345 1144
Fragments arranged by size:
1345 1144 752 391 174 27
EagI C'GGCC_G
Cuts at: 1418 1418
Size: 3833
EarI CTCTTCn'nnn_
Cuts at: 285 1856 2066 285
Size: 1571 210 2052
Fragments arranged by size:
2052 1571 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 788 2041 2351 2497 3643 127
Size: 661 1253 310 146 1146 317
Fragments arranged by size:
1253 1146 661 317 310 146
EcoICRI GAG'CTC
Cuts at: 845 845
Size: 3833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 1658 2090 2837 3105 540
Size: 1118 432 747 268 1268
Fragments arranged by size:
1268 1118 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 972 972
Size: 3833
EcoRI G'AATT_C
Cuts at: 837 837
Size: 3833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3833
FspI TGC'GCA
Cuts at: 1614 1614
Size: 3833
HaeII r_GCGC'y
Cuts at: 1267 1515 2537 3516 1267
Size: 248 1022 979 1584
Fragments arranged by size:
1584 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 509 541 1675 1705 1707 1737 190
Size: 32 287 32 1134 30 2 30 2286
Fragments arranged by size:
2286 1134 287 32 32 30 30 2
HincII GTy'rAC
Cuts at: 156 872 156
Size: 716 3117
KasI G'GCGC_C
Cuts at: 1263 1511 1263
Size: 248 3585
KpnI G_GTAC'C
Cuts at: 853 853
Size: 3833
MluI A'CGCG_T
Cuts at: 3266 3266
Size: 3833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2688 2504
Size: 184 3649
MscI TGG'CCA
Cuts at: 1594 1594
Size: 3833
MslI CAynn'nnrTG
Cuts at: 341 1822 1949 341
Size: 1481 127 2225
Fragments arranged by size:
2225 1481 127
MspA1I CmG'CkG
Cuts at: 944 1258 1618 2631 2876 944
Size: 314 360 1013 245 1901
Fragments arranged by size:
1901 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 3833
NarI GG'CG_CC
Cuts at: 1264 1512 1264
Size: 248 3585
NcoI C'CATG_G
Cuts at: 107 107
Size: 3833
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 3833
NsiI A_TGCA'T
Cuts at: 626 626
Size: 3833
NspI r_CATG'y
Cuts at: 188 886 1386 2293 188
Size: 698 500 907 1728
Fragments arranged by size:
1728 907 698 500
PciI A'CATG_T
Cuts at: 2289 2289
Size: 3833
PflMI CCAn_nnn'nTGG
Cuts at: 435 435
Size: 3833
PmeI GTTT'AAAC
Cuts at: 832 832
Size: 3833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 3801
PshAI GACnn'nnGTC
Cuts at: 734 734
Size: 3833
PstI C_TGCA'G
Cuts at: 880 1565 880
Size: 685 3148
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3833
PvuII CAG'CTG
Cuts at: 1258 1618 1258
Size: 360 3473
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 3833
SacI G_AGCT'C
Cuts at: 847 847
Size: 3833
SalI G'TCGA_C
Cuts at: 870 870
Size: 3833
SapI GCTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
SbfI CC_TGCA'GG
Cuts at: 880 880
Size: 3833
ScaI AGT'ACT
Cuts at: 3350 3350
Size: 3833
SexAI A'CCwGG_T
Cuts at: 431 431
Size: 3833
SfcI C'TryA_G
Cuts at: 33 876 1561 2554 2745 33
Size: 843 685 993 191 1121
Fragments arranged by size:
1121 993 843 685 191
SfoI GGC'GCC
Cuts at: 1265 1513 1265
Size: 248 3585
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
SmaI CCC'GGG
Cuts at: 706 855 706
Size: 149 3684
SmlI C'TyrA_G
Cuts at: 988 1903 2395 2657 988
Size: 915 492 262 2164
Fragments arranged by size:
2164 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3833
SphI G_CATG'C
Cuts at: 886 1386 886
Size: 500 3333
StyI C'CwwG_G
Cuts at: 107 967 1321 107
Size: 860 354 2619
Fragments arranged by size:
2619 860 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 710 1416 1549 2008 2217 710
Size: 706 133 459 209 2326
Fragments arranged by size:
2326 706 459 209 133
TatI w'GTAC_w
Cuts at: 3348 3348
Size: 3833
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 3801
Tth111I GACn'n_nGTC
Cuts at: 151 1630 151
Size: 1479 2354
XbaI T'CTAG_A
Cuts at: 724 864 724
Size: 140 3693
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1328 1328
Size: 3833
XmaI C'CCGG_G
Cuts at: 704 853 704
Size: 149 3684
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI