Restriction map for
pF1KE0660
(Circular) (Six-base) MAPSORT of: pF1KE0660.seq Check: 9821 from: 1 to: 4280
pF1KE0660 4280 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 1318 1318
Size: 4280
Acc65I G'GTAC_C
Cuts at: 1296 1296
Size: 4280
AclI AA'CG_TT
Cuts at: 4120 4120
Size: 4280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 342 876 1212 2105 2537 3284 3552 201
Size: 141 534 336 893 432 747 268 929
Fragments arranged by size:
929 893 747 534 432 336 268 141
AflIII A'CryG_T
Cuts at: 1632 2736 3713 1632
Size: 1104 977 2199
Fragments arranged by size:
2199 1104 977
AgeI A'CCGG_T
Cuts at: 2668 2668
Size: 4280
AleI CACnn'nnGTG
Cuts at: 1105 1105
Size: 4280
AlwNI CAG_nnn'CTG
Cuts at: 1585 3152 1585
Size: 1567 2713
ApaLI G'TGCA_C
Cuts at: 3050 3050
Size: 4280
ApoI r'AATT_y
Cuts at: 372 1284 3633 3889 372
Size: 912 2349 256 763
Fragments arranged by size:
2349 912 763 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4280
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
AvaI C'yCGr_G
Cuts at: 1300 1300
Size: 4280
BamHI G'GATC_C
Cuts at: 171 1305 171
Size: 1134 3146
BanI G'GyrC_C
Cuts at: 1296 1710 1958 1993 1296
Size: 414 248 35 3583
Fragments arranged by size:
3583 414 248 35
BanII G_rGCy'C
Cuts at: 1294 2324 1294
Size: 1030 3250
BbeI G_GCGC'C
Cuts at: 1714 1962 1714
Size: 248 4032
BbsI GAAGACnn'nnnn_
Cuts at: 257 432 257
Size: 175 4105
BciVI GTATCCnnnnn_n'
Cuts at: 2178 2939 4280 2178
Size: 761 1341 2178
Fragments arranged by size:
2178 1341 761
BclI T'GATC_A
Cuts at: 1800 1800
Size: 4280
BfrBI ATG'CAT
Cuts at: 784 784
Size: 4280
BglII A'GATC_T
Cuts at: 841 1795 3832 841
Size: 954 2037 1289
Fragments arranged by size:
2037 1289 954
BlpI GC'TnA_GC
Cuts at: 1392 1392
Size: 4280
Bme1580I G_kGCm'C
Cuts at: 1161 1905 1998 3054 1161
Size: 744 93 1056 2387
Fragments arranged by size:
2387 1056 744 93
BmrI ACTGGGnnnn_n'
Cuts at: 1492 1573 1908 4052 1492
Size: 81 335 2144 1720
Fragments arranged by size:
2144 1720 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 704 788 872 956 1124 1208 422
Size: 282 84 84 84 168 84 3494
Fragments arranged by size:
3494 282 168 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 2335 2827 3125 1456
Size: 879 492 298 2611
Fragments arranged by size:
2611 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3984 3984
Size: 4280
BsaAI yAC'GTr
Cuts at: 1633 1633
Size: 4280
BsaBI GATnn'nnATC
Cuts at: 6 1794 3831 6
Size: 1788 2037 455
Fragments arranged by size:
2037 1788 455
BsaHI Gr'CG_yC
Cuts at: 1711 1959 2661 4157 1711
Size: 248 702 1496 1834
Fragments arranged by size:
1834 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1469 1990 2668 2942 3089 3500 1469
Size: 521 678 274 147 411 2249
Fragments arranged by size:
2249 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 4280
BseYI C'CCAG_C
Cuts at: 1705 2463 3040 3536 1705
Size: 758 577 496 2449
Fragments arranged by size:
2449 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1822 1868 3076 104
Size: 1718 46 1208 1308
Fragments arranged by size:
1718 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 676 1294 2072 2262 3054 676
Size: 618 778 190 792 1902
Fragments arranged by size:
1902 792 778 618 190
BsmI GAATG_Cn'
Cuts at: 424 424
Size: 4280
Bsp1286I G_dGCh'C
Cuts at: 676 1161 1294 1905 1998 2072 2262 2324 3054
Size: 485 133 611 93 74 190 62 730
Cuts at: 3054 676
Size: 1902
Fragments arranged by size:
1902 730 611 485 190 133 93 74
62
BspEI T'CCGG_A
Cuts at: 3500 3500
Size: 4280
BspHI T'CATG_A
Cuts at: 3456 3456
Size: 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1330 1544 1846 2227 1330
Size: 214 302 381 3383
Fragments arranged by size:
3383 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2572 2626 4110 4275 50
Size: 2522 54 1484 165 55
Fragments arranged by size:
2522 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 531 2192 531
Size: 1661 2619
BsrFI r'CCGG_y
Cuts at: 2278 2459 2668 2278
Size: 181 209 3890
Fragments arranged by size:
3890 209 181
BssSI C'ACGA_G
Cuts at: 2551 2909 2551
Size: 358 3922
BstAPI GCAn_nnn'nTGC
Cuts at: 1657 1657
Size: 4280
BstBI TT'CG_AA
Cuts at: 1164 1248 2641 1164
Size: 84 1393 2803
Fragments arranged by size:
2803 1393 84
BstXI CCAn_nnnn'nTGG
Cuts at: 909 909
Size: 4280
BstYI r'GATC_y
Cuts at: 1 171 841 1305 1772 1795 2130 2376 3388
Size: 170 670 464 467 23 335 246 1012
Cuts at: 3388 3474 3486 3832 1
Size: 86 12 346 449
Fragments arranged by size:
1012 670 467 464 449 346 335 246
170 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2230 2411 2574 2230
Size: 181 163 3936
Fragments arranged by size:
3936 181 163
BtsI GCAGTG_nn'
Cuts at: 429 1523 429
Size: 1094 3186
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 525 560 525
Size: 35 4245
DraI TTT'AAA
Cuts at: 1279 1279
Size: 4280
DrdI GACnn_nn'nnGTC
Cuts at: 1986 2844 1986
Size: 858 3422
EaeI y'GGCC_r
Cuts at: 1865 2039 2430 2457 3802 1865
Size: 174 391 27 1345 2343
Fragments arranged by size:
2343 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1865 1865
Size: 4280
EarI CTCTTCn'nnn_
Cuts at: 830 2303 2513 830
Size: 1473 210 2597
Fragments arranged by size:
2597 1473 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2488 2798 2944 4090 2488
Size: 310 146 1146 2678
Fragments arranged by size:
2678 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1292 1292
Size: 4280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 342 422 704 788 872 876 956 1124
Size: 141 80 282 84 84 4 80 168
Cuts at: 1124 1208 1212 2105 2537 3284 3552 201
Size: 84 4 893 432 747 268 929
Fragments arranged by size:
929 893 747 432 282 268 168 141
84 84 84 80 80 4 4
EcoO109I rG'GnC_Cy
Cuts at: 1419 1419
Size: 4280
EcoRI G'AATT_C
Cuts at: 1284 1284
Size: 4280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 4280
FspI TGC'GCA
Cuts at: 2061 2061
Size: 4280
HaeII r_GCGC'y
Cuts at: 1714 1962 2984 3963 1714
Size: 248 1022 979 2031
Fragments arranged by size:
2031 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 2122 2152 2154 2184 103
Size: 32 1987 30 2 30 2199
Fragments arranged by size:
2199 1987 32 30 30 2
HincII GTy'rAC
Cuts at: 576 1319 576
Size: 743 3537
KasI G'GCGC_C
Cuts at: 1710 1958 1710
Size: 248 4032
KpnI G_GTAC'C
Cuts at: 1300 1300
Size: 4280
MluI A'CGCG_T
Cuts at: 3713 3713
Size: 4280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2951 3135 2951
Size: 184 4096
MscI TGG'CCA
Cuts at: 2041 2041
Size: 4280
MslI CAynn'nnrTG
Cuts at: 417 699 1105 2269 2396 417
Size: 282 406 1164 127 2301
Fragments arranged by size:
2301 1164 406 282 127
MspA1I CmG'CkG
Cuts at: 1391 1705 2065 3078 3323 1391
Size: 314 360 1013 245 2348
Fragments arranged by size:
2348 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2461 2461
Size: 4280
NarI GG'CG_CC
Cuts at: 1711 1959 1711
Size: 248 4032
NcoI C'CATG_G
Cuts at: 107 107
Size: 4280
NdeI CA'TA_TG
Cuts at: 445 445
Size: 4280
NgoMIV G'CCGG_C
Cuts at: 2459 2459
Size: 4280
NsiI A_TGCA'T
Cuts at: 786 786
Size: 4280
NspI r_CATG'y
Cuts at: 788 1333 1833 2740 788
Size: 545 500 907 2328
Fragments arranged by size:
2328 907 545 500
PciI A'CATG_T
Cuts at: 2736 2736
Size: 4280
PmeI GTTT'AAAC
Cuts at: 1279 1279
Size: 4280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 4248
PsiI TTA'TAA
Cuts at: 614 614
Size: 4280
PstI C_TGCA'G
Cuts at: 1327 2012 1327
Size: 685 3595
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4280
PvuII CAG'CTG
Cuts at: 1705 2065 1705
Size: 360 3920
RsrII CG'GwC_CG
Cuts at: 2475 2475
Size: 4280
SacI G_AGCT'C
Cuts at: 1294 1294
Size: 4280
SalI G'TCGA_C
Cuts at: 1317 1317
Size: 4280
SapI GCTCTTCn'nnn_
Cuts at: 2303 2513 2303
Size: 210 4070
SbfI CC_TGCA'GG
Cuts at: 1327 1327
Size: 4280
ScaI AGT'ACT
Cuts at: 3797 3797
Size: 4280
SfcI C'TryA_G
Cuts at: 33 192 233 1323 2008 3001 3192 33
Size: 159 41 1090 685 993 191 1121
Fragments arranged by size:
1121 1090 993 685 191 159 41
SfoI GGC'GCC
Cuts at: 1712 1960 1712
Size: 248 4032
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
SmaI CCC'GGG
Cuts at: 1302 1302
Size: 4280
SmlI C'TyrA_G
Cuts at: 1435 2350 2842 3104 1435
Size: 915 492 262 2611
Fragments arranged by size:
2611 915 492 262
SpeI A'CTAG_T
Cuts at: 67 598 67
Size: 531 3749
SphI G_CATG'C
Cuts at: 1333 1833 1333
Size: 500 3780
SspI AAT'ATT
Cuts at: 1066 1066
Size: 4280
StyI C'CwwG_G
Cuts at: 107 1414 1768 107
Size: 1307 354 2619
Fragments arranged by size:
2619 1307 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1863 1996 2455 2664 1863
Size: 133 459 209 3479
Fragments arranged by size:
3479 459 209 133
TatI w'GTAC_w
Cuts at: 3795 3795
Size: 4280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1002 1034 3797 3829 1002
Size: 32 2763 32 1453
Fragments arranged by size:
2763 1453 32 32
Tth111I GACn'n_nGTC
Cuts at: 2077 2077
Size: 4280
XbaI T'CTAG_A
Cuts at: 1311 1311
Size: 4280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1775 1775
Size: 4280
XmaI C'CCGG_G
Cuts at: 1300 1300
Size: 4280
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI