Restriction map for
pF1KE0657
(Circular) (Six-base) MAPSORT of: pF1KE0657.seq Check: 1339 from: 1 to: 3812
pF1KE0657 3812 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 850 850
Size: 3812
Acc65I G'GTAC_C
Cuts at: 828 828
Size: 3812
AclI AA'CG_TT
Cuts at: 3652 3652
Size: 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 262 1637 2069 2816 3084 133
Size: 129 1375 432 747 268 861
Fragments arranged by size:
1375 861 747 432 268 129
AflIII A'CryG_T
Cuts at: 800 1164 2268 3245 800
Size: 364 1104 977 1367
Fragments arranged by size:
1367 1104 977 364
AgeI A'CCGG_T
Cuts at: 2200 2200
Size: 3812
AlwNI CAG_nnn'CTG
Cuts at: 1117 2684 1117
Size: 1567 2245
ApaLI G'TGCA_C
Cuts at: 247 2582 247
Size: 2335 1477
ApoI r'AATT_y
Cuts at: 355 492 816 3165 3421 355
Size: 137 324 2349 256 746
Fragments arranged by size:
2349 746 324 256 137
AseI AT'TA_AT
Cuts at: 20 20
Size: 3812
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
AvaI C'yCGr_G
Cuts at: 425 832 425
Size: 407 3405
BamHI G'GATC_C
Cuts at: 837 837
Size: 3812
BanI G'GyrC_C
Cuts at: 828 1242 1490 1525 828
Size: 414 248 35 3115
Fragments arranged by size:
3115 414 248 35
BanII G_rGCy'C
Cuts at: 826 1856 826
Size: 1030 2782
BbeI G_GCGC'C
Cuts at: 1246 1494 1246
Size: 248 3564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 343 377 90
Size: 34 219 34 3525
Fragments arranged by size:
3525 219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1710 2471 3812 1710
Size: 761 1341 1710
Fragments arranged by size:
1710 1341 761
BclI T'GATC_A
Cuts at: 1332 1332
Size: 3812
BfrBI ATG'CAT
Cuts at: 324 324
Size: 3812
BglII A'GATC_T
Cuts at: 1327 3364 1327
Size: 2037 1775
BlpI GC'TnA_GC
Cuts at: 924 924
Size: 3812
Bme1580I G_kGCm'C
Cuts at: 251 1437 1530 2586 251
Size: 1186 93 1056 1477
Fragments arranged by size:
1477 1186 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1024 1105 1440 3584 1024
Size: 81 335 2144 1252
Fragments arranged by size:
2144 1252 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 3812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 545 254
Size: 291 3521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 988 1867 2359 2657 616
Size: 372 879 492 298 1771
Fragments arranged by size:
1771 879 492 372 298
BsaI GGTCTCn'nnnn_
Cuts at: 557 3516 557
Size: 2959 853
BsaAI yAC'GTr
Cuts at: 1165 1165
Size: 3812
BsaBI GATnn'nnATC
Cuts at: 6 1326 3363 6
Size: 1320 2037 455
Fragments arranged by size:
2037 1320 455
BsaHI Gr'CG_yC
Cuts at: 729 1243 1491 2193 3689 729
Size: 514 248 702 1496 852
Fragments arranged by size:
1496 852 702 514 248
BsaWI w'CCGG_w
Cuts at: 1001 1522 2200 2474 2621 3032 1001
Size: 521 678 274 147 411 1781
Fragments arranged by size:
1781 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 117 147 578 608 117
Size: 30 431 30 3321
Fragments arranged by size:
3321 431 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 575 575
Size: 3812
BseYI C'CCAG_C
Cuts at: 1237 1995 2572 3068 1237
Size: 758 577 496 1981
Fragments arranged by size:
1981 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1354 1400 2608 104
Size: 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 46
BsiHKAI G_wGCw'C
Cuts at: 251 826 1604 1794 2586 251
Size: 575 778 190 792 1477
Fragments arranged by size:
1477 792 778 575 190
Bsp1286I G_dGCh'C
Cuts at: 251 826 1437 1530 1604 1794 1856 2586 251
Size: 575 611 93 74 190 62 730 1477
Fragments arranged by size:
1477 730 611 575 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3032 3032
Size: 3812
BspHI T'CATG_A
Cuts at: 2988 2988
Size: 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 862 1076 1378 1759 862
Size: 214 302 381 2915
Fragments arranged by size:
2915 381 302 214
BsrBI CCG'CTC
Cuts at: 50 174 2104 2158 3642 3807 50
Size: 124 1930 54 1484 165 55
Fragments arranged by size:
1930 1484 165 124 55 54
BsrDI GCAATG_nn'
Cuts at: 1724 1724
Size: 3812
BsrFI r'CCGG_y
Cuts at: 1810 1991 2200 1810
Size: 181 209 3422
Fragments arranged by size:
3422 209 181
BsrGI T'GTAC_A
Cuts at: 292 292
Size: 3812
BssSI C'ACGA_G
Cuts at: 2083 2441 2083
Size: 358 3454
BstAPI GCAn_nnn'nTGC
Cuts at: 388 397 747 1189 388
Size: 9 350 442 3011
Fragments arranged by size:
3011 442 350 9
BstBI TT'CG_AA
Cuts at: 2173 2173
Size: 3812
BstYI r'GATC_y
Cuts at: 1 837 1304 1327 1662 1908 2920 3006 3018
Size: 836 467 23 335 246 1012 86 12
Cuts at: 3018 3364 1
Size: 346 449
Fragments arranged by size:
1012 836 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 137 137
Size: 3812
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1762 1943 2106 1762
Size: 181 163 3468
Fragments arranged by size:
3468 181 163
BtsI GCAGTG_nn'
Cuts at: 1055 1055
Size: 3812
DraI TTT'AAA
Cuts at: 811 811
Size: 3812
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2376 1518
Size: 858 2954
EaeI y'GGCC_r
Cuts at: 1397 1571 1962 1989 3334 1397
Size: 174 391 27 1345 1875
Fragments arranged by size:
1875 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1397 1397
Size: 3812
EarI CTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2020 2330 2476 3622 2020
Size: 310 146 1146 2210
Fragments arranged by size:
2210 1146 310 146
EcoICRI GAG'CTC
Cuts at: 824 824
Size: 3812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 254 262 545 1637 2069 2816 3084 133
Size: 121 8 283 1092 432 747 268 861
Fragments arranged by size:
1092 861 747 432 283 268 121 8
EcoO109I rG'GnC_Cy
Cuts at: 951 951
Size: 3812
EcoRI G'AATT_C
Cuts at: 355 816 355
Size: 461 3351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1831 1831
Size: 3812
FspI TGC'GCA
Cuts at: 1593 1593
Size: 3812
HaeII r_GCGC'y
Cuts at: 1246 1494 2516 3495 1246
Size: 248 1022 979 1563
Fragments arranged by size:
1563 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1654 1684 1686 1716 1654
Size: 30 2 30 3750
Fragments arranged by size:
3750 30 30 2
HincII GTy'rAC
Cuts at: 851 851
Size: 3812
KasI G'GCGC_C
Cuts at: 1242 1490 1242
Size: 248 3564
KpnI G_GTAC'C
Cuts at: 832 832
Size: 3812
MfeI C'AATT_G
Cuts at: 203 745 203
Size: 542 3270
MluI A'CGCG_T
Cuts at: 3245 3245
Size: 3812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2483 2667 2483
Size: 184 3628
MscI TGG'CCA
Cuts at: 1573 1573
Size: 3812
MslI CAynn'nnrTG
Cuts at: 1801 1928 1801
Size: 127 3685
MspA1I CmG'CkG
Cuts at: 570 923 1237 1597 2610 2855 570
Size: 353 314 360 1013 245 1527
Fragments arranged by size:
1527 1013 360 353 314 245
NaeI GCC'GGC
Cuts at: 1993 1993
Size: 3812
NarI GG'CG_CC
Cuts at: 1243 1491 1243
Size: 248 3564
NgoMIV G'CCGG_C
Cuts at: 1991 1991
Size: 3812
NsiI A_TGCA'T
Cuts at: 326 326
Size: 3812
NspI r_CATG'y
Cuts at: 804 865 1365 2272 804
Size: 61 500 907 2344
Fragments arranged by size:
2344 907 500 61
PciI A'CATG_T
Cuts at: 800 2268 800
Size: 1468 2344
PfoI T'CCnGG_A
Cuts at: 522 522
Size: 3812
PmeI GTTT'AAAC
Cuts at: 811 811
Size: 3812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 3780
PshAI GACnn'nnGTC
Cuts at: 163 163
Size: 3812
PspXI vC'TCGA_Gb
Cuts at: 425 425
Size: 3812
PstI C_TGCA'G
Cuts at: 859 1544 859
Size: 685 3127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3812
PvuII CAG'CTG
Cuts at: 570 1237 1597 570
Size: 667 360 2785
Fragments arranged by size:
2785 667 360
RsrII CG'GwC_CG
Cuts at: 2007 2007
Size: 3812
SacI G_AGCT'C
Cuts at: 826 826
Size: 3812
SalI G'TCGA_C
Cuts at: 849 849
Size: 3812
SapI GCTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
SbfI CC_TGCA'GG
Cuts at: 859 859
Size: 3812
ScaI AGT'ACT
Cuts at: 3329 3329
Size: 3812
SfcI C'TryA_G
Cuts at: 33 223 855 1540 2533 2724 33
Size: 190 632 685 993 191 1121
Fragments arranged by size:
1121 993 685 632 191 190
SfoI GGC'GCC
Cuts at: 1244 1492 1244
Size: 248 3564
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
SmaI CCC'GGG
Cuts at: 834 834
Size: 3812
SmlI C'TyrA_G
Cuts at: 425 631 967 1882 2374 2636 425
Size: 206 336 915 492 262 1601
Fragments arranged by size:
1601 915 492 336 262 206
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3812
SphI G_CATG'C
Cuts at: 865 1365 865
Size: 500 3312
StuI AGG'CCT
Cuts at: 458 551 458
Size: 93 3719
StyI C'CwwG_G
Cuts at: 459 946 1300 459
Size: 487 354 2971
Fragments arranged by size:
2971 487 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1395 1528 1987 2196 1395
Size: 133 459 209 3011
Fragments arranged by size:
3011 459 209 133
TatI w'GTAC_w
Cuts at: 292 3327 292
Size: 3035 777
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 117 149 578 610 3329 3361 117
Size: 32 429 32 2719 32 568
Fragments arranged by size:
2719 568 429 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 649 1609 649
Size: 960 2852
XbaI T'CTAG_A
Cuts at: 843 843
Size: 3812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 144 1307 144
Size: 1163 2649
XhoI C'TCGA_G
Cuts at: 425 425
Size: 3812
XmaI C'CCGG_G
Cuts at: 832 832
Size: 3812
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PshAI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI