Restriction map for
pF1KE0656
(Circular) (Six-base) MAPSORT of: pF1KE0656.seq Check: 9027 from: 1 to: 4277
pF1KE0656 4277 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1052 1052
Size: 4277
AccI GT'mk_AC
Cuts at: 1315 1315
Size: 4277
Acc65I G'GTAC_C
Cuts at: 1293 1293
Size: 4277
AclI AA'CG_TT
Cuts at: 4117 4117
Size: 4277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 811 907 944 2102 2534 3281 3549 517
Size: 294 96 37 1158 432 747 268 1245
Fragments arranged by size:
1245 1158 747 432 294 268 96 37
AflIII A'CryG_T
Cuts at: 140 142 962 1629 2733 3710 140
Size: 2 820 667 1104 977 707
Fragments arranged by size:
1104 977 820 707 667 2
AgeI A'CCGG_T
Cuts at: 2665 2665
Size: 4277
AleI CACnn'nnGTG
Cuts at: 473 473
Size: 4277
AlwNI CAG_nnn'CTG
Cuts at: 1582 3149 1582
Size: 1567 2710
ApaLI G'TGCA_C
Cuts at: 3047 3047
Size: 4277
ApoI r'AATT_y
Cuts at: 133 228 910 1084 1281 3630 3886 133
Size: 95 682 174 197 2349 256 524
Fragments arranged by size:
2349 682 524 256 197 174 95
AseI AT'TA_AT
Cuts at: 20 20
Size: 4277
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
AvaI C'yCGr_G
Cuts at: 1297 1297
Size: 4277
AvrII C'CTAG_G
Cuts at: 441 441
Size: 4277
BamHI G'GATC_C
Cuts at: 1302 1302
Size: 4277
BanI G'GyrC_C
Cuts at: 1293 1707 1955 1990 1293
Size: 414 248 35 3580
Fragments arranged by size:
3580 414 248 35
BanII G_rGCy'C
Cuts at: 1291 2321 1291
Size: 1030 3247
BbeI G_GCGC'C
Cuts at: 1711 1959 1711
Size: 248 4029
BciVI GTATCCnnnnn_n'
Cuts at: 2175 2936 4277 2175
Size: 761 1341 2175
Fragments arranged by size:
2175 1341 761
BclI T'GATC_A
Cuts at: 372 1797 372
Size: 1425 2852
BfrBI ATG'CAT
Cuts at: 762 762
Size: 4277
BglII A'GATC_T
Cuts at: 250 1792 3829 250
Size: 1542 2037 698
Fragments arranged by size:
2037 1542 698
BlpI GC'TnA_GC
Cuts at: 827 1389 827
Size: 562 3715
Bme1580I G_kGCm'C
Cuts at: 1902 1995 3051 1902
Size: 93 1056 3128
Fragments arranged by size:
3128 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1489 1570 1905 4049 1489
Size: 81 335 2144 1717
Fragments arranged by size:
2144 1717 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1137 2644 1137
Size: 1507 2770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1194 184
Size: 1010 3267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 1453 2332 2824 3122 1159
Size: 294 879 492 298 2314
Fragments arranged by size:
2314 879 492 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 3981 3981
Size: 4277
BsaAI yAC'GTr
Cuts at: 143 1630 143
Size: 1487 2790
BsaBI GATnn'nnATC
Cuts at: 6 1791 3828 6
Size: 1785 2037 455
Fragments arranged by size:
2037 1785 455
BsaHI Gr'CG_yC
Cuts at: 1708 1956 2658 4154 1708
Size: 248 702 1496 1831
Fragments arranged by size:
1831 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1466 1987 2665 2939 3086 3497 1466
Size: 521 678 274 147 411 2246
Fragments arranged by size:
2246 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 265 295 265
Size: 30 4247
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 347 461 493 559 1117 347
Size: 114 32 66 558 3507
Fragments arranged by size:
3507 558 114 66 32
BseYI C'CCAG_C
Cuts at: 1702 2460 3037 3533 1702
Size: 758 577 496 2446
Fragments arranged by size:
2446 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 207
Size: 4277
BsiEI CG_ry'CG
Cuts at: 104 1819 1865 3073 104
Size: 1715 46 1208 1308
Fragments arranged by size:
1715 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1291 2069 2259 3051 1291
Size: 778 190 792 2517
Fragments arranged by size:
2517 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 475 1030 475
Size: 555 3722
Bsp1286I G_dGCh'C
Cuts at: 1291 1902 1995 2069 2259 2321 3051 1291
Size: 611 93 74 190 62 730 2517
Fragments arranged by size:
2517 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3497 3497
Size: 4277
BspHI T'CATG_A
Cuts at: 569 728 3453 569
Size: 159 2725 1393
Fragments arranged by size:
2725 1393 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1052 1327 1541 1843 2224 1052
Size: 275 214 302 381 3105
Fragments arranged by size:
3105 381 302 275 214
BsrBI CCG'CTC
Cuts at: 50 403 2569 2623 4107 4272 50
Size: 353 2166 54 1484 165 55
Fragments arranged by size:
2166 1484 353 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2189 2189
Size: 4277
BsrFI r'CCGG_y
Cuts at: 2275 2456 2665 2275
Size: 181 209 3887
Fragments arranged by size:
3887 209 181
BsrGI T'GTAC_A
Cuts at: 770 770
Size: 4277
BssSI C'ACGA_G
Cuts at: 2548 2906 2548
Size: 358 3919
BstAPI GCAn_nnn'nTGC
Cuts at: 1654 1654
Size: 4277
BstBI TT'CG_AA
Cuts at: 2638 2638
Size: 4277
BstYI r'GATC_y
Cuts at: 1 250 1302 1769 1792 2127 2373 3385 3471
Size: 249 1052 467 23 335 246 1012 86
Cuts at: 3471 3483 3829 1
Size: 12 346 449
Fragments arranged by size:
1052 1012 467 449 346 335 249 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 966 2227 2408 2571 966
Size: 1261 181 163 2672
Fragments arranged by size:
2672 1261 181 163
BtsI GCAGTG_nn'
Cuts at: 1520 1520
Size: 4277
ClaI AT'CG_AT
Cuts at: 686 686
Size: 4277
DraI TTT'AAA
Cuts at: 1276 1276
Size: 4277
DrdI GACnn_nn'nnGTC
Cuts at: 1983 2841 1983
Size: 858 3419
EaeI y'GGCC_r
Cuts at: 459 485 857 1862 2036 2427 2454 3799 459
Size: 26 372 1005 174 391 27 1345 937
Fragments arranged by size:
1345 1005 937 391 372 174 27 26
EagI C'GGCC_G
Cuts at: 1862 1862
Size: 4277
EarI CTCTTCn'nnn_
Cuts at: 115 188 1138 2300 2510 115
Size: 73 950 1162 210 1882
Fragments arranged by size:
1882 1162 950 210 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2485 2795 2941 4087 2485
Size: 310 146 1146 2675
Fragments arranged by size:
2675 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1289 1289
Size: 4277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 517 811 907 944 1194 2102 2534 3281
Size: 333 294 96 37 250 908 432 747
Cuts at: 3281 3549 184
Size: 268 912
Fragments arranged by size:
912 908 747 432 333 294 268 250
96 37
EcoO109I rG'GnC_Cy
Cuts at: 1416 1416
Size: 4277
EcoRI G'AATT_C
Cuts at: 133 1281 133
Size: 1148 3129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 912 2296 912
Size: 1384 2893
FspI TGC'GCA
Cuts at: 2058 2058
Size: 4277
HaeII r_GCGC'y
Cuts at: 1711 1959 2981 3960 1711
Size: 248 1022 979 2028
Fragments arranged by size:
2028 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 265 267 297 2119 2149 2151 2181 235
Size: 30 2 30 1822 30 2 30 2331
Fragments arranged by size:
2331 1822 30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 345 408 1316 345
Size: 63 908 3306
Fragments arranged by size:
3306 908 63
HindIII A'AGCT_T
Cuts at: 1126 1126
Size: 4277
KasI G'GCGC_C
Cuts at: 1707 1955 1707
Size: 248 4029
KpnI G_GTAC'C
Cuts at: 1297 1297
Size: 4277
MluI A'CGCG_T
Cuts at: 3710 3710
Size: 4277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2948 3132 2948
Size: 184 4093
MscI TGG'CCA
Cuts at: 859 2038 859
Size: 1179 3098
MslI CAynn'nnrTG
Cuts at: 473 961 2266 2393 473
Size: 488 1305 127 2357
Fragments arranged by size:
2357 1305 488 127
MspA1I CmG'CkG
Cuts at: 162 579 603 1388 1702 2062 3075 3320 162
Size: 417 24 785 314 360 1013 245 1119
Fragments arranged by size:
1119 1013 785 417 360 314 245 24
NaeI GCC'GGC
Cuts at: 2458 2458
Size: 4277
NarI GG'CG_CC
Cuts at: 1708 1956 1708
Size: 248 4029
NgoMIV G'CCGG_C
Cuts at: 2456 2456
Size: 4277
NsiI A_TGCA'T
Cuts at: 764 764
Size: 4277
NspI r_CATG'y
Cuts at: 762 966 1330 1830 2737 762
Size: 204 364 500 907 2302
Fragments arranged by size:
2302 907 500 364 204
PciI A'CATG_T
Cuts at: 962 2733 962
Size: 1771 2506
PmeI GTTT'AAAC
Cuts at: 1276 1276
Size: 4277
PmlI CAC'GTG
Cuts at: 143 143
Size: 4277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 4245
PsiI TTA'TAA
Cuts at: 149 149
Size: 4277
PstI C_TGCA'G
Cuts at: 1063 1324 2009 1063
Size: 261 685 3331
Fragments arranged by size:
3331 685 261
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4277
PvuII CAG'CTG
Cuts at: 162 579 603 1702 2062 162
Size: 417 24 1099 360 2377
Fragments arranged by size:
2377 1099 417 360 24
RsrII CG'GwC_CG
Cuts at: 2472 2472
Size: 4277
SacI G_AGCT'C
Cuts at: 1291 1291
Size: 4277
SalI G'TCGA_C
Cuts at: 1314 1314
Size: 4277
SapI GCTCTTCn'nnn_
Cuts at: 2300 2510 2300
Size: 210 4067
SbfI CC_TGCA'GG
Cuts at: 1063 1324 1063
Size: 261 4016
ScaI AGT'ACT
Cuts at: 3794 3794
Size: 4277
SfcI C'TryA_G
Cuts at: 33 1059 1320 2005 2998 3189 33
Size: 1026 261 685 993 191 1121
Fragments arranged by size:
1121 1026 993 685 261 191
SfoI GGC'GCC
Cuts at: 1709 1957 1709
Size: 248 4029
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
SmaI CCC'GGG
Cuts at: 1299 1299
Size: 4277
SmlI C'TyrA_G
Cuts at: 1174 1432 2347 2839 3101 1174
Size: 258 915 492 262 2350
Fragments arranged by size:
2350 915 492 262 258
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4277
SphI G_CATG'C
Cuts at: 1330 1830 1330
Size: 500 3777
StyI C'CwwG_G
Cuts at: 441 1109 1411 1765 441
Size: 668 302 354 2953
Fragments arranged by size:
2953 668 354 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1009 1860 1993 2452 2661 1009
Size: 851 133 459 209 2625
Fragments arranged by size:
2625 851 459 209 133
TatI w'GTAC_w
Cuts at: 770 3792 770
Size: 3022 1255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4245
Tth111I GACn'n_nGTC
Cuts at: 634 2074 634
Size: 1440 2837
XbaI T'CTAG_A
Cuts at: 1308 1308
Size: 4277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1772 1772
Size: 4277
XmaI C'CCGG_G
Cuts at: 1297 1297
Size: 4277
XmnI GAAnn'nnTTC
Cuts at: 1085 1085
Size: 4277
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PmeI PmlI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI