Restriction map for
pF1KE0654
(Circular) (Six-base) MAPSORT of: pF1KE0654.seq Check: 1888 from: 1 to: 4271
pF1KE0654 4271 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 755 1309 755
Size: 554 3717
Acc65I G'GTAC_C
Cuts at: 1287 1287
Size: 4271
AclI AA'CG_TT
Cuts at: 4111 4111
Size: 4271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 2096 2528 3275 3543 485
Size: 1611 432 747 268 1213
Fragments arranged by size:
1611 1213 747 432 268
AflIII A'CryG_T
Cuts at: 1623 2727 3704 1623
Size: 1104 977 2190
Fragments arranged by size:
2190 1104 977
AgeI A'CCGG_T
Cuts at: 2659 2659
Size: 4271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 296 1112 1234 296
Size: 816 122 3333
Fragments arranged by size:
3333 816 122
AlwNI CAG_nnn'CTG
Cuts at: 1576 3143 1576
Size: 1567 2704
ApaLI G'TGCA_C
Cuts at: 3041 3041
Size: 4271
ApoI r'AATT_y
Cuts at: 833 1275 3624 3880 833
Size: 442 2349 256 1224
Fragments arranged by size:
2349 1224 442 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4271
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
AvaI C'yCGr_G
Cuts at: 512 1291 512
Size: 779 3492
BamHI G'GATC_C
Cuts at: 1296 1296
Size: 4271
BanI G'GyrC_C
Cuts at: 1287 1701 1949 1984 1287
Size: 414 248 35 3574
Fragments arranged by size:
3574 414 248 35
BanII G_rGCy'C
Cuts at: 1285 2315 1285
Size: 1030 3241
BbeI G_GCGC'C
Cuts at: 1705 1953 1705
Size: 248 4023
BbsI GAAGACnn'nnnn_
Cuts at: 636 1145 636
Size: 509 3762
BciVI GTATCCnnnnn_n'
Cuts at: 222 2169 2930 4271 222
Size: 1947 761 1341 222
Fragments arranged by size:
1947 1341 761 222
BclI T'GATC_A
Cuts at: 1791 1791
Size: 4271
BglI GCCn_nnn'nGGC
Cuts at: 1034 1200 1034
Size: 166 4105
BglII A'GATC_T
Cuts at: 1786 3823 1786
Size: 2037 2234
BlpI GC'TnA_GC
Cuts at: 1383 1383
Size: 4271
Bme1580I G_kGCm'C
Cuts at: 1896 1989 3045 1896
Size: 93 1056 3122
Fragments arranged by size:
3122 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 657 1483 1564 1899 4043 657
Size: 826 81 335 2144 885
Fragments arranged by size:
2144 885 826 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1181 1447 2326 2818 3116 1181
Size: 266 879 492 298 2336
Fragments arranged by size:
2336 879 492 298 266
BsaI GGTCTCn'nnnn_
Cuts at: 3975 3975
Size: 4271
BsaAI yAC'GTr
Cuts at: 1624 1624
Size: 4271
BsaBI GATnn'nnATC
Cuts at: 6 1785 3822 6
Size: 1779 2037 455
Fragments arranged by size:
2037 1779 455
BsaHI Gr'CG_yC
Cuts at: 1702 1950 2652 4148 1702
Size: 248 702 1496 1825
Fragments arranged by size:
1825 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1460 1981 2659 2933 3080 3491 1460
Size: 521 678 274 147 411 2240
Fragments arranged by size:
2240 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1696 2454 3031 3527 1696
Size: 758 577 496 2440
Fragments arranged by size:
2440 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 1142 911
Size: 231 4040
BsiEI CG_ry'CG
Cuts at: 104 1813 1859 3067 104
Size: 1709 46 1208 1308
Fragments arranged by size:
1709 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1285 2063 2253 3045 1285
Size: 778 190 792 2511
Fragments arranged by size:
2511 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1285 1896 1989 2063 2253 2315 3045 1285
Size: 611 93 74 190 62 730 2511
Fragments arranged by size:
2511 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3491 3491
Size: 4271
BspHI T'CATG_A
Cuts at: 267 3447 267
Size: 3180 1091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1321 1535 1837 2218 1321
Size: 214 302 381 3374
Fragments arranged by size:
3374 381 302 214
BsrBI CCG'CTC
Cuts at: 50 670 2563 2617 4101 4266 50
Size: 620 1893 54 1484 165 55
Fragments arranged by size:
1893 1484 620 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2183 2183
Size: 4271
BsrFI r'CCGG_y
Cuts at: 2269 2450 2659 2269
Size: 181 209 3881
Fragments arranged by size:
3881 209 181
BssSI C'ACGA_G
Cuts at: 2542 2900 2542
Size: 358 3913
BstAPI GCAn_nnn'nTGC
Cuts at: 298 898 1648 298
Size: 600 750 2921
Fragments arranged by size:
2921 750 600
BstBI TT'CG_AA
Cuts at: 2632 2632
Size: 4271
BstEII G'GTnAC_C
Cuts at: 142 142
Size: 4271
BstXI CCAn_nnnn'nTGG
Cuts at: 622 1024 1045 622
Size: 402 21 3848
Fragments arranged by size:
3848 402 21
BstYI r'GATC_y
Cuts at: 1 1296 1763 1786 2121 2367 3379 3465 3477
Size: 1295 467 23 335 246 1012 86 12
Cuts at: 3477 3823 1
Size: 346 449
Fragments arranged by size:
1295 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 756 756
Size: 4271
BtgI C'CryG_G
Cuts at: 107 1162 107
Size: 1055 3216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2221 2402 2565 2221
Size: 181 163 3927
Fragments arranged by size:
3927 181 163
BtsI GCAGTG_nn'
Cuts at: 1514 1514
Size: 4271
DraI TTT'AAA
Cuts at: 1270 1270
Size: 4271
DrdI GACnn_nn'nnGTC
Cuts at: 1977 2835 1977
Size: 858 3413
EaeI y'GGCC_r
Cuts at: 612 1026 1065 1113 1856 2030 2421 2448 3793
Size: 414 39 48 743 174 391 27 1345
Cuts at: 3793 612
Size: 1090
Fragments arranged by size:
1345 1090 743 414 391 174 48 39
27
EagI C'GGCC_G
Cuts at: 1856 1856
Size: 4271
EarI CTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2479 2789 2935 4081 2479
Size: 310 146 1146 2669
Fragments arranged by size:
2669 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1283 1283
Size: 4271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 2096 2528 3275 3543 485
Size: 1611 432 747 268 1213
Fragments arranged by size:
1611 1213 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1410 1410
Size: 4271
EcoRI G'AATT_C
Cuts at: 1275 1275
Size: 4271
EcoRV GAT'ATC
Cuts at: 1012 1012
Size: 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 985 2290 985
Size: 1305 2966
FspI TGC'GCA
Cuts at: 2052 2052
Size: 4271
HaeII r_GCGC'y
Cuts at: 1705 1953 2975 3954 1705
Size: 248 1022 979 2022
Fragments arranged by size:
2022 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 153 185 2113 2143 2145 2175 153
Size: 32 1928 30 2 30 2249
Fragments arranged by size:
2249 1928 32 30 30 2
HincII GTy'rAC
Cuts at: 1310 1310
Size: 4271
KasI G'GCGC_C
Cuts at: 1701 1949 1701
Size: 248 4023
KpnI G_GTAC'C
Cuts at: 1291 1291
Size: 4271
MfeI C'AATT_G
Cuts at: 902 902
Size: 4271
MluI A'CGCG_T
Cuts at: 3704 3704
Size: 4271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2942 3126 2942
Size: 184 4087
MscI TGG'CCA
Cuts at: 614 1028 1067 1115 2032 614
Size: 414 39 48 917 2853
Fragments arranged by size:
2853 917 414 48 39
MslI CAynn'nnrTG
Cuts at: 278 425 887 2260 2387 278
Size: 147 462 1373 127 2162
Fragments arranged by size:
2162 1373 462 147 127
MspA1I CmG'CkG
Cuts at: 235 286 1382 1696 2056 3069 3314 235
Size: 51 1096 314 360 1013 245 1192
Fragments arranged by size:
1192 1096 1013 360 314 245 51
NaeI GCC'GGC
Cuts at: 2452 2452
Size: 4271
NarI GG'CG_CC
Cuts at: 1702 1950 1702
Size: 248 4023
NcoI C'CATG_G
Cuts at: 107 1162 107
Size: 1055 3216
NdeI CA'TA_TG
Cuts at: 684 711 1101 684
Size: 27 390 3854
Fragments arranged by size:
3854 390 27
NgoMIV G'CCGG_C
Cuts at: 2450 2450
Size: 4271
NspI r_CATG'y
Cuts at: 901 1218 1324 1824 2731 901
Size: 317 106 500 907 2441
Fragments arranged by size:
2441 907 500 317 106
PciI A'CATG_T
Cuts at: 2727 2727
Size: 4271
PflMI CCAn_nnn'nTGG
Cuts at: 1180 1180
Size: 4271
PmeI GTTT'AAAC
Cuts at: 1270 1270
Size: 4271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 4239
PstI C_TGCA'G
Cuts at: 291 1318 2003 291
Size: 1027 685 2559
Fragments arranged by size:
2559 1027 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4271
PvuII CAG'CTG
Cuts at: 235 286 1696 2056 235
Size: 51 1410 360 2450
Fragments arranged by size:
2450 1410 360 51
RsrII CG'GwC_CG
Cuts at: 2466 2466
Size: 4271
SacI G_AGCT'C
Cuts at: 1285 1285
Size: 4271
SalI G'TCGA_C
Cuts at: 1308 1308
Size: 4271
SapI GCTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
SbfI CC_TGCA'GG
Cuts at: 1318 1318
Size: 4271
ScaI AGT'ACT
Cuts at: 3788 3788
Size: 4271
SfcI C'TryA_G
Cuts at: 33 287 1314 1999 2992 3183 33
Size: 254 1027 685 993 191 1121
Fragments arranged by size:
1121 1027 993 685 254 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1034 1034
Size: 4271
SfoI GGC'GCC
Cuts at: 1703 1951 1703
Size: 248 4023
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
SmaI CCC'GGG
Cuts at: 1293 1293
Size: 4271
SmlI C'TyrA_G
Cuts at: 1196 1426 2341 2833 3095 1196
Size: 230 915 492 262 2372
Fragments arranged by size:
2372 915 492 262 230
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4271
SphI G_CATG'C
Cuts at: 1324 1824 1324
Size: 500 3771
StyI C'CwwG_G
Cuts at: 107 1162 1405 1759 107
Size: 1055 243 354 2619
Fragments arranged by size:
2619 1055 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 598 1854 1987 2446 2655 598
Size: 1256 133 459 209 2214
Fragments arranged by size:
2214 1256 459 209 133
TatI w'GTAC_w
Cuts at: 679 748 3786 679
Size: 69 3038 1164
Fragments arranged by size:
3038 1164 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3788 3820 3788
Size: 32 4239
Tth111I GACn'n_nGTC
Cuts at: 1149 2068 1149
Size: 919 3352
XbaI T'CTAG_A
Cuts at: 1302 1302
Size: 4271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 433 603 622 1766 433
Size: 170 19 1144 2938
Fragments arranged by size:
2938 1144 170 19
XmaI C'CCGG_G
Cuts at: 1291 1291
Size: 4271
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI