Restriction map for
pF1KE0653
(Circular) (Six-base) MAPSORT of: pF1KE0653.seq Check: 3689 from: 1 to: 3776
pF1KE0653 3776 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 814 814
Size: 3776
Acc65I G'GTAC_C
Cuts at: 792 792
Size: 3776
AclI AA'CG_TT
Cuts at: 3616 3616
Size: 3776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1601 2033 2780 3048 508
Size: 1093 432 747 268 1236
Fragments arranged by size:
1236 1093 747 432 268
AflIII A'CryG_T
Cuts at: 1128 2232 3209 1128
Size: 1104 977 1695
Fragments arranged by size:
1695 1104 977
AgeI A'CCGG_T
Cuts at: 2164 2164
Size: 3776
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 190 222 190
Size: 32 3744
AlwNI CAG_nnn'CTG
Cuts at: 486 1081 2648 486
Size: 595 1567 1614
Fragments arranged by size:
1614 1567 595
ApaLI G'TGCA_C
Cuts at: 2546 2546
Size: 3776
ApoI r'AATT_y
Cuts at: 613 780 3129 3385 613
Size: 167 2349 256 1004
Fragments arranged by size:
2349 1004 256 167
AseI AT'TA_AT
Cuts at: 20 20
Size: 3776
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
AvaI C'yCGr_G
Cuts at: 293 796 293
Size: 503 3273
BamHI G'GATC_C
Cuts at: 801 801
Size: 3776
BanI G'GyrC_C
Cuts at: 792 1206 1454 1489 792
Size: 414 248 35 3079
Fragments arranged by size:
3079 414 248 35
BanII G_rGCy'C
Cuts at: 242 790 1820 242
Size: 548 1030 2198
Fragments arranged by size:
2198 1030 548
BbeI G_GCGC'C
Cuts at: 1210 1458 1210
Size: 248 3528
BbsI GAAGACnn'nnnn_
Cuts at: 581 581
Size: 3776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 162 196 162
Size: 34 3742
BciVI GTATCCnnnnn_n'
Cuts at: 1674 2435 3776 1674
Size: 761 1341 1674
Fragments arranged by size:
1674 1341 761
BclI T'GATC_A
Cuts at: 1296 1296
Size: 3776
BglII A'GATC_T
Cuts at: 430 1291 3328 430
Size: 861 2037 878
Fragments arranged by size:
2037 878 861
BlpI GC'TnA_GC
Cuts at: 888 888
Size: 3776
Bme1580I G_kGCm'C
Cuts at: 266 1401 1494 2550 266
Size: 1135 93 1056 1492
Fragments arranged by size:
1492 1135 1056 93
BmgBI CAC'GTC
Cuts at: 180 180
Size: 3776
BmrI ACTGGGnnnn_n'
Cuts at: 988 1069 1404 3548 988
Size: 81 335 2144 1216
Fragments arranged by size:
2144 1216 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 3776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 952 1831 2323 2621 226
Size: 726 879 492 298 1381
Fragments arranged by size:
1381 879 726 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 695 3480 695
Size: 2785 991
BsaAI yAC'GTr
Cuts at: 159 1129 159
Size: 970 2806
BsaBI GATnn'nnATC
Cuts at: 6 1290 3327 6
Size: 1284 2037 455
Fragments arranged by size:
2037 1284 455
BsaHI Gr'CG_yC
Cuts at: 1207 1455 2157 3653 1207
Size: 248 702 1496 1330
Fragments arranged by size:
1496 1330 702 248
BsaWI w'CCGG_w
Cuts at: 965 1486 2164 2438 2585 2996 965
Size: 521 678 274 147 411 1745
Fragments arranged by size:
1745 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 190 220 190
Size: 30 3746
BseYI C'CCAG_C
Cuts at: 116 508 1201 1959 2536 3032 116
Size: 392 693 758 577 496 860
Fragments arranged by size:
860 758 693 577 496 392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 553
Size: 3776
BsiEI CG_ry'CG
Cuts at: 104 176 222 1318 1364 2572 104
Size: 72 46 1096 46 1208 1308
Fragments arranged by size:
1308 1208 1096 72 46 46
BsiHKAI G_wGCw'C
Cuts at: 242 790 1568 1758 2550 242
Size: 548 778 190 792 1468
Fragments arranged by size:
1468 792 778 548 190
Bsp1286I G_dGCh'C
Cuts at: 242 266 790 1401 1494 1568 1758 1820 2550
Size: 24 524 611 93 74 190 62 730
Cuts at: 2550 242
Size: 1468
Fragments arranged by size:
1468 730 611 524 190 93 74 62
24
BspEI T'CCGG_A
Cuts at: 2996 2996
Size: 3776
BspHI T'CATG_A
Cuts at: 624 2952 624
Size: 2328 1448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 826 1040 1342 1723 826
Size: 214 302 381 2879
Fragments arranged by size:
2879 381 302 214
BsrBI CCG'CTC
Cuts at: 50 620 2068 2122 3606 3771 50
Size: 570 1448 54 1484 165 55
Fragments arranged by size:
1484 1448 570 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1688 1688
Size: 3776
BsrFI r'CCGG_y
Cuts at: 248 1774 1955 2164 248
Size: 1526 181 209 1860
Fragments arranged by size:
1860 1526 209 181
BssSI C'ACGA_G
Cuts at: 259 2047 2405 259
Size: 1788 358 1630
Fragments arranged by size:
1788 1630 358
BstAPI GCAn_nnn'nTGC
Cuts at: 307 1153 307
Size: 846 2930
BstBI TT'CG_AA
Cuts at: 2137 2137
Size: 3776
BstYI r'GATC_y
Cuts at: 1 430 801 1268 1291 1626 1872 2884 2970
Size: 429 371 467 23 335 246 1012 86
Cuts at: 2970 2982 3328 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 429 371 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 399 399
Size: 3776
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1726 1907 2070 1726
Size: 181 163 3432
Fragments arranged by size:
3432 181 163
BtsI GCAGTG_nn'
Cuts at: 1019 1019
Size: 3776
DraI TTT'AAA
Cuts at: 775 775
Size: 3776
DrdI GACnn_nn'nnGTC
Cuts at: 1482 2340 1482
Size: 858 2918
EaeI y'GGCC_r
Cuts at: 219 339 1361 1535 1926 1953 3298 219
Size: 120 1022 174 391 27 1345 697
Fragments arranged by size:
1345 1022 697 391 174 120 27
EagI C'GGCC_G
Cuts at: 219 1361 219
Size: 1142 2634
EarI CTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 1984 2294 2440 3586 136
Size: 1848 310 146 1146 326
Fragments arranged by size:
1848 1146 326 310 146
EcoICRI GAG'CTC
Cuts at: 240 788 240
Size: 548 3228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1601 2033 2780 3048 508
Size: 1093 432 747 268 1236
Fragments arranged by size:
1236 1093 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 467 467
Size: 3776
EcoO109I rG'GnC_Cy
Cuts at: 201 288 483 764 915 201
Size: 87 195 281 151 3062
Fragments arranged by size:
3062 281 195 151 87
EcoRI G'AATT_C
Cuts at: 780 780
Size: 3776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1795 1795
Size: 3776
FspI TGC'GCA
Cuts at: 1557 1557
Size: 3776
HaeII r_GCGC'y
Cuts at: 1210 1458 2480 3459 1210
Size: 248 1022 979 1527
Fragments arranged by size:
1527 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1618 1648 1650 1680 1618
Size: 30 2 30 3714
Fragments arranged by size:
3714 30 30 2
HincII GTy'rAC
Cuts at: 815 815
Size: 3776
KasI G'GCGC_C
Cuts at: 1206 1454 1206
Size: 248 3528
KpnI G_GTAC'C
Cuts at: 796 796
Size: 3776
MluI A'CGCG_T
Cuts at: 3209 3209
Size: 3776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 196 2447 2631 196
Size: 2251 184 1341
Fragments arranged by size:
2251 1341 184
MscI TGG'CCA
Cuts at: 341 1537 341
Size: 1196 2580
MslI CAynn'nnrTG
Cuts at: 157 629 1765 1892 157
Size: 472 1136 127 2041
Fragments arranged by size:
2041 1136 472 127
MspA1I CmG'CkG
Cuts at: 476 887 1201 1561 2574 2819 476
Size: 411 314 360 1013 245 1433
Fragments arranged by size:
1433 1013 411 360 314 245
NaeI GCC'GGC
Cuts at: 1957 1957
Size: 3776
NarI GG'CG_CC
Cuts at: 1207 1455 1207
Size: 248 3528
NgoMIV G'CCGG_C
Cuts at: 1955 1955
Size: 3776
NspI r_CATG'y
Cuts at: 501 829 1329 2236 501
Size: 328 500 907 2041
Fragments arranged by size:
2041 907 500 328
PciI A'CATG_T
Cuts at: 2232 2232
Size: 3776
PmeI GTTT'AAAC
Cuts at: 775 775
Size: 3776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 190 222 2915 2947 190
Size: 32 2693 32 1019
Fragments arranged by size:
2693 1019 32 32
PpuMI rG'GwC_Cy
Cuts at: 201 288 483 201
Size: 87 195 3494
Fragments arranged by size:
3494 195 87
PstI C_TGCA'G
Cuts at: 823 1508 823
Size: 685 3091
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3776
PvuII CAG'CTG
Cuts at: 476 1201 1561 476
Size: 725 360 2691
Fragments arranged by size:
2691 725 360
RsrII CG'GwC_CG
Cuts at: 1971 1971
Size: 3776
SacI G_AGCT'C
Cuts at: 242 790 242
Size: 548 3228
SalI G'TCGA_C
Cuts at: 813 813
Size: 3776
SapI GCTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
SbfI CC_TGCA'GG
Cuts at: 823 823
Size: 3776
ScaI AGT'ACT
Cuts at: 3293 3293
Size: 3776
SfcI C'TryA_G
Cuts at: 33 819 1504 2497 2688 33
Size: 786 685 993 191 1121
Fragments arranged by size:
1121 993 786 685 191
SfoI GGC'GCC
Cuts at: 1208 1456 1208
Size: 248 3528
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
SmaI CCC'GGG
Cuts at: 798 798
Size: 3776
SmlI C'TyrA_G
Cuts at: 241 931 1846 2338 2600 241
Size: 690 915 492 262 1417
Fragments arranged by size:
1417 915 690 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3776
SphI G_CATG'C
Cuts at: 829 1329 829
Size: 500 3276
StyI C'CwwG_G
Cuts at: 910 1264 910
Size: 354 3422
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 190 380 683 1359 1492 1951 2160 190
Size: 190 303 676 133 459 209 1806
Fragments arranged by size:
1806 676 459 303 209 190 133
TatI w'GTAC_w
Cuts at: 3291 3291
Size: 3776
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 3744
Tth111I GACn'n_nGTC
Cuts at: 1573 1573
Size: 3776
XbaI T'CTAG_A
Cuts at: 807 807
Size: 3776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1271 1271
Size: 3776
XmaI C'CCGG_G
Cuts at: 796 796
Size: 3776
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI BpmI
Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI