Restriction map for
pF1KE0652
(Circular) (Six-base) MAPSORT of: pF1KE0652.seq Check: 5717 from: 1 to: 4268
pF1KE0652 4268 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1306 1306
Size: 4268
Acc65I G'GTAC_C
Cuts at: 1284 1284
Size: 4268
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 248 304 698 2093 2525 3272 3540 176
Size: 72 56 394 1395 432 747 268 904
Fragments arranged by size:
1395 904 747 432 394 268 72 56
AflIII A'CryG_T
Cuts at: 911 913 1620 2724 3701 911
Size: 2 707 1104 977 1478
Fragments arranged by size:
1478 1104 977 707 2
AgeI A'CCGG_T
Cuts at: 2656 2656
Size: 4268
AhdI GACnn_n'nnGTC
Cuts at: 720 720
Size: 4268
AleI CACnn'nnGTG
Cuts at: 1234 1234
Size: 4268
AlwNI CAG_nnn'CTG
Cuts at: 1573 3140 1573
Size: 1567 2701
ApaLI G'TGCA_C
Cuts at: 1246 3038 1246
Size: 1792 2476
ApoI r'AATT_y
Cuts at: 1272 3621 3877 1272
Size: 2349 256 1663
Fragments arranged by size:
2349 1663 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 113 1288 113
Size: 1175 3093
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 900 933 900
Size: 33 4235
BamHI G'GATC_C
Cuts at: 1293 1293
Size: 4268
BanI G'GyrC_C
Cuts at: 932 1152 1284 1698 1946 1981 932
Size: 220 132 414 248 35 3219
Fragments arranged by size:
3219 414 248 220 132 35
BanII G_rGCy'C
Cuts at: 1282 2312 1282
Size: 1030 3238
BbeI G_GCGC'C
Cuts at: 1702 1950 1702
Size: 248 4020
BbsI GAAGACnn'nnnn_
Cuts at: 164 368 164
Size: 204 4064
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 910 944 1094 1128 910
Size: 34 150 34 4050
Fragments arranged by size:
4050 150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 277 2166 2927 4268 277
Size: 1889 761 1341 277
Fragments arranged by size:
1889 1341 761 277
BclI T'GATC_A
Cuts at: 593 1788 593
Size: 1195 3073
BglII A'GATC_T
Cuts at: 170 1783 3820 170
Size: 1613 2037 618
Fragments arranged by size:
2037 1613 618
BlpI GC'TnA_GC
Cuts at: 1380 1380
Size: 4268
Bme1580I G_kGCm'C
Cuts at: 1250 1893 1986 3042 1250
Size: 643 93 1056 2476
Fragments arranged by size:
2476 1056 643 93
BmrI ACTGGGnnnn_n'
Cuts at: 849 1480 1561 1896 4040 849
Size: 631 81 335 2144 1077
Fragments arranged by size:
2144 1077 631 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 150 846 2635 150
Size: 696 1789 1783
Fragments arranged by size:
1789 1783 696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 644 644
Size: 4268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 793 1444 2323 2815 3113 526
Size: 267 651 879 492 298 1681
Fragments arranged by size:
1681 879 651 492 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 181 530 1200 1212 3972 181
Size: 349 670 12 2760 477
Fragments arranged by size:
2760 670 477 349 12
BsaAI yAC'GTr
Cuts at: 914 1621 914
Size: 707 3561
BsaBI GATnn'nnATC
Cuts at: 6 1782 3819 6
Size: 1776 2037 455
Fragments arranged by size:
2037 1776 455
BsaHI Gr'CG_yC
Cuts at: 497 1699 1947 2649 4145 497
Size: 1202 248 702 1496 620
Fragments arranged by size:
1496 1202 702 620 248
BsaWI w'CCGG_w
Cuts at: 412 431 998 1457 1978 2656 2930 3077 3488
Size: 19 567 459 521 678 274 147 411
Cuts at: 3488 412
Size: 1192
Fragments arranged by size:
1192 678 567 521 459 411 274 147
19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 433 463 433
Size: 30 4238
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 592 126
Size: 466 3802
BseYI C'CCAG_C
Cuts at: 1693 2451 3028 3524 1693
Size: 758 577 496 2437
Fragments arranged by size:
2437 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1049 1810 1856 3064 104
Size: 945 761 46 1208 1308
Fragments arranged by size:
1308 1208 945 761 46
BsiHKAI G_wGCw'C
Cuts at: 1250 1282 2060 2250 3042 1250
Size: 32 778 190 792 2476
Fragments arranged by size:
2476 792 778 190 32
Bsp1286I G_dGCh'C
Cuts at: 1250 1282 1893 1986 2060 2250 2312 3042 1250
Size: 32 611 93 74 190 62 730 2476
Fragments arranged by size:
2476 730 611 190 93 74 62 32
BspEI T'CCGG_A
Cuts at: 998 3488 998
Size: 2490 1778
BspHI T'CATG_A
Cuts at: 3444 3444
Size: 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1318 1532 1834 2215 1318
Size: 214 302 381 3371
Fragments arranged by size:
3371 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1215 2560 2614 4098 4263 50
Size: 1165 1345 54 1484 165 55
Fragments arranged by size:
1484 1345 1165 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2180 2180
Size: 4268
BsrFI r'CCGG_y
Cuts at: 2266 2447 2656 2266
Size: 181 209 3878
Fragments arranged by size:
3878 209 181
BssSI C'ACGA_G
Cuts at: 976 2539 2897 976
Size: 1563 358 2347
Fragments arranged by size:
2347 1563 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1039 1645 1039
Size: 606 3662
BstBI TT'CG_AA
Cuts at: 2629 2629
Size: 4268
BstYI r'GATC_y
Cuts at: 1 170 803 995 1293 1760 1783 2118 2364
Size: 169 633 192 298 467 23 335 246
Cuts at: 2364 3376 3462 3474 3820 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 633 467 449 346 335 298 246
192 169 86 23 12
BtgI C'CryG_G
Cuts at: 107 937 1043 107
Size: 830 106 3332
Fragments arranged by size:
3332 830 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2218 2399 2562 2218
Size: 181 163 3924
Fragments arranged by size:
3924 181 163
BtsI GCAGTG_nn'
Cuts at: 644 1250 1511 644
Size: 606 261 3401
Fragments arranged by size:
3401 606 261
DraI TTT'AAA
Cuts at: 1267 1267
Size: 4268
DrdI GACnn_nn'nnGTC
Cuts at: 1974 2832 1974
Size: 858 3410
EaeI y'GGCC_r
Cuts at: 1046 1130 1853 2027 2418 2445 3790 1046
Size: 84 723 174 391 27 1345 1524
Fragments arranged by size:
1524 1345 723 391 174 84 27
EagI C'GGCC_G
Cuts at: 1046 1853 1046
Size: 807 3461
EarI CTCTTCn'nnn_
Cuts at: 224 2291 2501 224
Size: 2067 210 1991
Fragments arranged by size:
2067 1991 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 472 829 2476 2786 2932 4078 472
Size: 357 1647 310 146 1146 662
Fragments arranged by size:
1647 1146 662 357 310 146
EcoICRI GAG'CTC
Cuts at: 1280 1280
Size: 4268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 248 304 644 698 2093 2525 3272 3540
Size: 72 56 340 54 1395 432 747 268
Cuts at: 3540 176
Size: 904
Fragments arranged by size:
1395 904 747 432 340 268 72 56
54
EcoO109I rG'GnC_Cy
Cuts at: 203 894 1407 203
Size: 691 513 3064
Fragments arranged by size:
3064 691 513
EcoRI G'AATT_C
Cuts at: 1272 1272
Size: 4268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 189 2287 189
Size: 2098 2170
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 283 992 1702 1950 2972 3951 283
Size: 709 710 248 1022 979 600
Fragments arranged by size:
1022 979 710 709 600 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 565 597 625 657 706 738 835 867 2110
Size: 32 28 32 49 32 97 32 1243
Cuts at: 2110 2140 2142 2172 565
Size: 30 2 30 2661
Fragments arranged by size:
2661 1243 97 49 32 32 32 32
30 30 28 2
HincII GTy'rAC
Cuts at: 714 1307 714
Size: 593 3675
KasI G'GCGC_C
Cuts at: 1698 1946 1698
Size: 248 4020
KpnI G_GTAC'C
Cuts at: 1288 1288
Size: 4268
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 878 1185 2939 3123 878
Size: 307 1754 184 2023
Fragments arranged by size:
2023 1754 307 184
MscI TGG'CCA
Cuts at: 1132 2029 1132
Size: 897 3371
MslI CAynn'nnrTG
Cuts at: 730 1234 2257 2384 730
Size: 504 1023 127 2614
Fragments arranged by size:
2614 1023 504 127
MspA1I CmG'CkG
Cuts at: 405 1379 1693 2053 3066 3311 405
Size: 974 314 360 1013 245 1362
Fragments arranged by size:
1362 1013 974 360 314 245
NaeI GCC'GGC
Cuts at: 2449 2449
Size: 4268
NarI GG'CG_CC
Cuts at: 1699 1947 1699
Size: 248 4020
NcoI C'CATG_G
Cuts at: 107 937 107
Size: 830 3438
NdeI CA'TA_TG
Cuts at: 1061 1061
Size: 4268
NgoMIV G'CCGG_C
Cuts at: 2447 2447
Size: 4268
NspI r_CATG'y
Cuts at: 1321 1821 2728 1321
Size: 500 907 2861
Fragments arranged by size:
2861 907 500
PciI A'CATG_T
Cuts at: 2724 2724
Size: 4268
PflMI CCAn_nnn'nTGG
Cuts at: 492 519 492
Size: 27 4241
PfoI T'CCnGG_A
Cuts at: 139 139
Size: 4268
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PmlI CAC'GTG
Cuts at: 914 914
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 4236
PpuMI rG'GwC_Cy
Cuts at: 894 894
Size: 4268
PstI C_TGCA'G
Cuts at: 1315 2000 1315
Size: 685 3583
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 405 1693 2053 405
Size: 1288 360 2620
Fragments arranged by size:
2620 1288 360
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 1282 1282
Size: 4268
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 2291 2501 2291
Size: 210 4058
SbfI CC_TGCA'GG
Cuts at: 1315 1315
Size: 4268
ScaI AGT'ACT
Cuts at: 3785 3785
Size: 4268
SfcI C'TryA_G
Cuts at: 33 564 1167 1311 1996 2989 3180 33
Size: 531 603 144 685 993 191 1121
Fragments arranged by size:
1121 993 685 603 531 191 144
SfoI GGC'GCC
Cuts at: 1700 1948 1700
Size: 248 4020
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 115 1290 115
Size: 1175 3093
SmlI C'TyrA_G
Cuts at: 541 808 1423 2338 2830 3092 541
Size: 267 615 915 492 262 1717
Fragments arranged by size:
1717 915 615 492 267 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4268
SphI G_CATG'C
Cuts at: 1321 1821 1321
Size: 500 3768
SspI AAT'ATT
Cuts at: 348 348
Size: 4268
StyI C'CwwG_G
Cuts at: 107 937 1402 1756 107
Size: 830 465 354 2619
Fragments arranged by size:
2619 830 465 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 950 1851 1984 2443 2652 950
Size: 901 133 459 209 2566
Fragments arranged by size:
2566 901 459 209 133
TatI w'GTAC_w
Cuts at: 389 503 560 3783 389
Size: 114 57 3223 874
Fragments arranged by size:
3223 874 114 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 933 965 3785 3817 933
Size: 32 2820 32 1384
Fragments arranged by size:
2820 1384 32 32
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 1299 1299
Size: 4268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1763 1763
Size: 4268
XmaI C'CCGG_G
Cuts at: 113 1288 113
Size: 1175 3093
XmnI GAAnn'nnTTC
Cuts at: 172 172
Size: 4268
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI