Restriction map for
pF1KE0648
(Circular) (Six-base) MAPSORT of: pF1KE0648.seq Check: 8682 from: 1 to: 4253
pF1KE0648 4253 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1130 1291 1130
Size: 161 4092
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 340 708 2078 2510 3257 3525 313
Size: 27 368 1370 432 747 268 1041
Fragments arranged by size:
1370 1041 747 432 368 268 27
AflIII A'CryG_T
Cuts at: 1605 2709 3686 1605
Size: 1104 977 2172
Fragments arranged by size:
2172 1104 977
AgeI A'CCGG_T
Cuts at: 2641 2641
Size: 4253
AhdI GACnn_n'nnGTC
Cuts at: 1047 1047
Size: 4253
AlwNI CAG_nnn'CTG
Cuts at: 168 714 1558 3125 168
Size: 546 844 1567 1296
Fragments arranged by size:
1567 1296 844 546
ApaI G_GGCC'C
Cuts at: 929 1242 929
Size: 313 3940
ApaLI G'TGCA_C
Cuts at: 3023 3023
Size: 4253
ApoI r'AATT_y
Cuts at: 1257 3606 3862 1257
Size: 2349 256 1648
Fragments arranged by size:
2349 1648 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 802 827 1273 802
Size: 25 446 3782
Fragments arranged by size:
3782 446 25
BamHI G'GATC_C
Cuts at: 1278 1278
Size: 4253
BanI G'GyrC_C
Cuts at: 421 661 983 1191 1269 1683 1931 1966 421
Size: 240 322 208 78 414 248 35 2708
Fragments arranged by size:
2708 414 322 248 240 208 78 35
BanII G_rGCy'C
Cuts at: 304 688 749 929 1242 1267 2297 304
Size: 384 61 180 313 25 1030 2260
Fragments arranged by size:
2260 1030 384 313 180 61 25
BbeI G_GCGC'C
Cuts at: 425 665 1195 1687 1935 425
Size: 240 530 492 248 2743
Fragments arranged by size:
2743 530 492 248 240
BbsI GAAGACnn'nnnn_
Cuts at: 361 361
Size: 4253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 607 641 607
Size: 34 4219
BciVI GTATCCnnnnn_n'
Cuts at: 2151 2912 4253 2151
Size: 761 1341 2151
Fragments arranged by size:
2151 1341 761
BclI T'GATC_A
Cuts at: 1773 1773
Size: 4253
BglI GCCn_nnn'nGGC
Cuts at: 475 839 475
Size: 364 3889
BglII A'GATC_T
Cuts at: 1768 3805 1768
Size: 2037 2216
BlpI GC'TnA_GC
Cuts at: 670 1365 670
Size: 695 3558
Bme1580I G_kGCm'C
Cuts at: 929 1242 1878 1971 3027 929
Size: 313 636 93 1056 2155
Fragments arranged by size:
2155 1056 636 313 93
BmgBI CAC'GTC
Cuts at: 218 218
Size: 4253
BmrI ACTGGGnnnn_n'
Cuts at: 1465 1546 1881 4025 1465
Size: 81 335 2144 1693
Fragments arranged by size:
2144 1693 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4253
Bpu10I CC'TnA_GC
Cuts at: 646 646
Size: 4253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 2308 2800 3098 1429
Size: 879 492 298 2584
Fragments arranged by size:
2584 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 760 3957 760
Size: 3197 1056
BsaAI yAC'GTr
Cuts at: 273 861 1606 273
Size: 588 745 2920
Fragments arranged by size:
2920 745 588
BsaBI GATnn'nnATC
Cuts at: 6 1767 3804 6
Size: 1761 2037 455
Fragments arranged by size:
2037 1761 455
BsaHI Gr'CG_yC
Cuts at: 395 422 662 1192 1684 1932 2634 4130 395
Size: 27 240 530 492 248 702 1496 518
Fragments arranged by size:
1496 702 530 518 492 248 240 27
BsaWI w'CCGG_w
Cuts at: 1442 1963 2641 2915 3062 3473 1442
Size: 521 678 274 147 411 2222
Fragments arranged by size:
2222 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 478 508 478
Size: 30 4223
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 622 754 757 622
Size: 132 3 4118
Fragments arranged by size:
4118 132 3
BseYI C'CCAG_C
Cuts at: 152 385 1057 1678 2436 3013 3509 152
Size: 233 672 621 758 577 496 896
Fragments arranged by size:
896 758 672 621 577 496 233
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 558 874 1132 558
Size: 316 258 3679
Fragments arranged by size:
3679 316 258
BsiEI CG_ry'CG
Cuts at: 104 429 1795 1841 3049 104
Size: 325 1366 46 1208 1308
Fragments arranged by size:
1366 1308 1208 325 46
BsiHKAI G_wGCw'C
Cuts at: 749 1214 1267 2045 2235 3027 749
Size: 465 53 778 190 792 1975
Fragments arranged by size:
1975 792 778 465 190 53
Bsp1286I G_dGCh'C
Cuts at: 304 688 749 929 1214 1242 1267 1878 1971
Size: 384 61 180 285 28 25 611 93
Cuts at: 1971 2045 2235 2297 3027 304
Size: 74 190 62 730 1530
Fragments arranged by size:
1530 730 611 384 285 190 180 93
74 62 61 28 25
BspEI T'CCGG_A
Cuts at: 3473 3473
Size: 4253
BspHI T'CATG_A
Cuts at: 3429 3429
Size: 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1236 1303 1517 1819 2200 1236
Size: 67 214 302 381 3289
Fragments arranged by size:
3289 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 189 279 483 1011 2545 2599 4083 4248
Size: 139 90 204 528 1534 54 1484 165
Cuts at: 4248 50
Size: 55
Fragments arranged by size:
1534 1484 528 204 165 139 90 55
54
BsrDI GCAATG_nn'
Cuts at: 681 2165 681
Size: 1484 2769
BsrFI r'CCGG_y
Cuts at: 2251 2432 2641 2251
Size: 181 209 3863
Fragments arranged by size:
3863 209 181
BssHII G'CGCG_C
Cuts at: 362 362
Size: 4253
BssSI C'ACGA_G
Cuts at: 307 1117 2524 2882 307
Size: 810 1407 358 1678
Fragments arranged by size:
1678 1407 810 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1630 1630
Size: 4253
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstXI CCAn_nnnn'nTGG
Cuts at: 273 273
Size: 4253
BstYI r'GATC_y
Cuts at: 1 1278 1745 1768 2103 2349 3361 3447 3459
Size: 1277 467 23 335 246 1012 86 12
Cuts at: 3459 3805 1
Size: 346 449
Fragments arranged by size:
1277 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 770 1160 770
Size: 390 3863
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2203 2384 2547 2203
Size: 181 163 3909
Fragments arranged by size:
3909 181 163
BtsI GCAGTG_nn'
Cuts at: 1496 1496
Size: 4253
DraI TTT'AAA
Cuts at: 1252 1252
Size: 4253
DrdI GACnn_nn'nnGTC
Cuts at: 1959 2817 1959
Size: 858 3395
EaeI y'GGCC_r
Cuts at: 239 476 678 1838 2012 2403 2430 3775 239
Size: 237 202 1160 174 391 27 1345 717
Fragments arranged by size:
1345 1160 717 391 237 202 174 27
EagI C'GGCC_G
Cuts at: 1838 1838
Size: 4253
EarI CTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 2771 2917 4063 2461
Size: 310 146 1146 2651
Fragments arranged by size:
2651 1146 310 146
EcoICRI GAG'CTC
Cuts at: 747 1265 747
Size: 518 3735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 340 708 2078 2510 3257 3525 313
Size: 27 368 1370 432 747 268 1041
Fragments arranged by size:
1370 1041 747 432 368 268 27
EcoNI CCTnn'n_nnAGG
Cuts at: 295 505 655 295
Size: 210 150 3893
Fragments arranged by size:
3893 210 150
EcoO109I rG'GnC_Cy
Cuts at: 926 1238 1239 1392 926
Size: 312 1 153 3787
Fragments arranged by size:
3787 312 153 1
EcoRI G'AATT_C
Cuts at: 1257 1257
Size: 4253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 4253
FspI TGC'GCA
Cuts at: 655 899 2034 655
Size: 244 1135 2874
Fragments arranged by size:
2874 1135 244
HaeII r_GCGC'y
Cuts at: 425 665 945 1152 1195 1687 1935 2957 3936
Size: 240 280 207 43 492 248 1022 979
Cuts at: 3936 425
Size: 742
Fragments arranged by size:
1022 979 742 492 280 248 240 207
43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 513 1033 1065 2095 2125 2127 2157 481
Size: 32 520 32 1030 30 2 30 2577
Fragments arranged by size:
2577 1030 520 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1292 1292
Size: 4253
HindIII A'AGCT_T
Cuts at: 639 639
Size: 4253
KasI G'GCGC_C
Cuts at: 421 661 1191 1683 1931 421
Size: 240 530 492 248 2743
Fragments arranged by size:
2743 530 492 248 240
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 290 617 2924 3108 290
Size: 327 2307 184 1435
Fragments arranged by size:
2307 1435 327 184
MscI TGG'CCA
Cuts at: 680 2014 680
Size: 1334 2919
MslI CAynn'nnrTG
Cuts at: 526 775 2242 2369 526
Size: 249 1467 127 2410
Fragments arranged by size:
2410 1467 249 127
MspA1I CmG'CkG
Cuts at: 419 938 1146 1364 1678 2038 3051 3296 419
Size: 519 208 218 314 360 1013 245 1376
Fragments arranged by size:
1376 1013 519 360 314 245 218 208
NaeI GCC'GGC
Cuts at: 2434 2434
Size: 4253
NarI GG'CG_CC
Cuts at: 422 662 1192 1684 1932 422
Size: 240 530 492 248 2743
Fragments arranged by size:
2743 530 492 248 240
NcoI C'CATG_G
Cuts at: 770 1160 770
Size: 390 3863
NgoMIV G'CCGG_C
Cuts at: 2432 2432
Size: 4253
NspI r_CATG'y
Cuts at: 703 1306 1806 2713 703
Size: 603 500 907 2243
Fragments arranged by size:
2243 907 603 500
PciI A'CATG_T
Cuts at: 2709 2709
Size: 4253
PflMI CCAn_nnn'nTGG
Cuts at: 168 218 1161 168
Size: 50 943 3260
Fragments arranged by size:
3260 943 50
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 4221
PspOMI G'GGCC_C
Cuts at: 925 1238 925
Size: 313 3940
PstI C_TGCA'G
Cuts at: 1247 1300 1985 1247
Size: 53 685 3515
Fragments arranged by size:
3515 685 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 1678 2038 1678
Size: 360 3893
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 749 1267 749
Size: 518 3735
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SapI GCTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
SbfI CC_TGCA'GG
Cuts at: 1247 1300 1247
Size: 53 4200
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SfcI C'TryA_G
Cuts at: 33 1243 1296 1981 2974 3165 33
Size: 1210 53 685 993 191 1121
Fragments arranged by size:
1210 1121 993 685 191 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 839 839
Size: 4253
SfoI GGC'GCC
Cuts at: 423 663 1193 1685 1933 423
Size: 240 530 492 248 2743
Fragments arranged by size:
2743 530 492 248 240
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 829 1275 829
Size: 446 3807
SmlI C'TyrA_G
Cuts at: 1408 2323 2815 3077 1408
Size: 915 492 262 2584
Fragments arranged by size:
2584 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 1306 1806 1306
Size: 500 3753
StuI AGG'CCT
Cuts at: 335 335
Size: 4253
StyI C'CwwG_G
Cuts at: 770 1160 1387 1741 770
Size: 390 227 354 3282
Fragments arranged by size:
3282 390 354 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1836 1969 2428 2637 1836
Size: 133 459 209 3452
Fragments arranged by size:
3452 459 209 133
TatI w'GTAC_w
Cuts at: 3768 3768
Size: 4253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3770 3802 3770
Size: 32 4221
Tth111I GACn'n_nGTC
Cuts at: 1126 2050 1126
Size: 924 3329
XbaI T'CTAG_A
Cuts at: 1284 1284
Size: 4253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 600 858 1167 1748 600
Size: 258 309 581 3105
Fragments arranged by size:
3105 581 309 258
XmaI C'CCGG_G
Cuts at: 827 1273 827
Size: 446 3807
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmtI BpmI BsiWI BsmI
BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI