Restriction map for
pF1KE0639
(Circular) (Six-base) MAPSORT of: pF1KE0639.seq Check: 6580 from: 1 to: 3755
pF1KE0639 3755 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 793 793
Size: 3755
Acc65I G'GTAC_C
Cuts at: 771 771
Size: 3755
AclI AA'CG_TT
Cuts at: 3595 3595
Size: 3755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 1580 2012 2759 3027 541
Size: 1039 432 747 268 1269
Fragments arranged by size:
1269 1039 747 432 268
AflIII A'CryG_T
Cuts at: 596 1107 2211 3188 596
Size: 511 1104 977 1163
Fragments arranged by size:
1163 1104 977 511
AgeI A'CCGG_T
Cuts at: 2143 2143
Size: 3755
AhdI GACnn_n'nnGTC
Cuts at: 696 696
Size: 3755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 448 448
Size: 3755
AlwNI CAG_nnn'CTG
Cuts at: 1060 2627 1060
Size: 1567 2188
ApaI G_GGCC'C
Cuts at: 119 119
Size: 3755
ApaLI G'TGCA_C
Cuts at: 2525 2525
Size: 3755
ApoI r'AATT_y
Cuts at: 759 3108 3364 759
Size: 2349 256 1150
Fragments arranged by size:
2349 1150 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
AvaI C'yCGr_G
Cuts at: 652 775 652
Size: 123 3632
BamHI G'GATC_C
Cuts at: 780 780
Size: 3755
BanI G'GyrC_C
Cuts at: 771 1185 1433 1468 771
Size: 414 248 35 3058
Fragments arranged by size:
3058 414 248 35
BanII G_rGCy'C
Cuts at: 119 547 769 1799 119
Size: 428 222 1030 2075
Fragments arranged by size:
2075 1030 428 222
BbeI G_GCGC'C
Cuts at: 1189 1437 1189
Size: 248 3507
BbsI GAAGACnn'nnnn_
Cuts at: 447 447
Size: 3755
BciVI GTATCCnnnnn_n'
Cuts at: 1653 2414 3755 1653
Size: 761 1341 1653
Fragments arranged by size:
1653 1341 761
BclI T'GATC_A
Cuts at: 1275 1275
Size: 3755
BglI GCCn_nnn'nGGC
Cuts at: 143 143
Size: 3755
BglII A'GATC_T
Cuts at: 1270 3307 1270
Size: 2037 1718
BlpI GC'TnA_GC
Cuts at: 867 867
Size: 3755
Bme1580I G_kGCm'C
Cuts at: 119 1380 1473 2529 119
Size: 1261 93 1056 1345
Fragments arranged by size:
1345 1261 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 967 1048 1383 3527 967
Size: 81 335 2144 1195
Fragments arranged by size:
2144 1195 335 81
BmtI G_CTAG'C
Cuts at: 476 476
Size: 3755
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 3755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 931 1810 2302 2600 289
Size: 642 879 492 298 1444
Fragments arranged by size:
1444 879 642 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 718 3459 718
Size: 2741 1014
BsaAI yAC'GTr
Cuts at: 179 621 1108 179
Size: 442 487 2826
Fragments arranged by size:
2826 487 442
BsaBI GATnn'nnATC
Cuts at: 6 298 1269 3306 6
Size: 292 971 2037 455
Fragments arranged by size:
2037 971 455 292
BsaHI Gr'CG_yC
Cuts at: 680 1186 1434 2136 3632 680
Size: 506 248 702 1496 803
Fragments arranged by size:
1496 803 702 506 248
BsaWI w'CCGG_w
Cuts at: 944 1465 2143 2417 2564 2975 944
Size: 521 678 274 147 411 1724
Fragments arranged by size:
1724 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 274
Size: 3755
BseYI C'CCAG_C
Cuts at: 122 1180 1938 2515 3011 122
Size: 1058 758 577 496 866
Fragments arranged by size:
1058 866 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 352
Size: 3755
BsiEI CG_ry'CG
Cuts at: 104 1297 1343 2551 104
Size: 1193 46 1208 1308
Fragments arranged by size:
1308 1208 1193 46
BsiHKAI G_wGCw'C
Cuts at: 547 626 769 1547 1737 2529 547
Size: 79 143 778 190 792 1773
Fragments arranged by size:
1773 792 778 190 143 79
BsiWI C'GTAC_G
Cuts at: 179 179
Size: 3755
BsmI GAATG_Cn'
Cuts at: 311 311
Size: 3755
Bsp1286I G_dGCh'C
Cuts at: 119 547 626 769 1380 1473 1547 1737 1799
Size: 428 79 143 611 93 74 190 62
Cuts at: 1799 2529 119
Size: 730 1345
Fragments arranged by size:
1345 730 611 428 190 143 93 79
74 62
BspEI T'CCGG_A
Cuts at: 2975 2975
Size: 3755
BspHI T'CATG_A
Cuts at: 2931 2931
Size: 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 1019 1321 1702 805
Size: 214 302 381 2858
Fragments arranged by size:
2858 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2047 2101 3585 3750 50
Size: 1997 54 1484 165 55
Fragments arranged by size:
1997 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1667 1667
Size: 3755
BsrFI r'CCGG_y
Cuts at: 1753 1934 2143 1753
Size: 181 209 3365
Fragments arranged by size:
3365 209 181
BsrGI T'GTAC_A
Cuts at: 191 191
Size: 3755
BssSI C'ACGA_G
Cuts at: 218 2026 2384 218
Size: 1808 358 1589
Fragments arranged by size:
1808 1589 358
BstAPI GCAn_nnn'nTGC
Cuts at: 489 1132 489
Size: 643 3112
BstBI TT'CG_AA
Cuts at: 2116 2116
Size: 3755
BstYI r'GATC_y
Cuts at: 1 299 516 780 1247 1270 1605 1851 2863
Size: 298 217 264 467 23 335 246 1012
Cuts at: 2863 2949 2961 3307 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 298 264 246
217 86 23 12
BtgI C'CryG_G
Cuts at: 107 185 604 627 701 107
Size: 78 419 23 74 3161
Fragments arranged by size:
3161 419 78 74 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1705 1886 2049 1705
Size: 181 163 3411
Fragments arranged by size:
3411 181 163
BtsI GCAGTG_nn'
Cuts at: 998 998
Size: 3755
DraI TTT'AAA
Cuts at: 754 754
Size: 3755
DraIII CAC_nnn'GTG
Cuts at: 330 330
Size: 3755
DrdI GACnn_nn'nnGTC
Cuts at: 1461 2319 1461
Size: 858 2897
EaeI y'GGCC_r
Cuts at: 1340 1514 1905 1932 3277 1340
Size: 174 391 27 1345 1818
Fragments arranged by size:
1818 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1340 1340
Size: 3755
EarI CTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1963 2273 2419 3565 1963
Size: 310 146 1146 2153
Fragments arranged by size:
2153 1146 310 146
EcoICRI GAG'CTC
Cuts at: 545 767 545
Size: 222 3533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 1580 2012 2759 3027 541
Size: 1039 432 747 268 1269
Fragments arranged by size:
1269 1039 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 115 127 894 115
Size: 12 767 2976
Fragments arranged by size:
2976 767 12
EcoRI G'AATT_C
Cuts at: 759 759
Size: 3755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1774 1774
Size: 3755
FspI TGC'GCA
Cuts at: 390 1536 390
Size: 1146 2609
HaeII r_GCGC'y
Cuts at: 165 479 1189 1437 2459 3438 165
Size: 314 710 248 1022 979 482
Fragments arranged by size:
1022 979 710 482 314 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 682 714 1597 1627 1629 1659 682
Size: 32 883 30 2 30 2778
Fragments arranged by size:
2778 883 32 30 30 2
HincII GTy'rAC
Cuts at: 794 794
Size: 3755
KasI G'GCGC_C
Cuts at: 1185 1433 1185
Size: 248 3507
KpnI G_GTAC'C
Cuts at: 775 775
Size: 3755
MluI A'CGCG_T
Cuts at: 3188 3188
Size: 3755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2610 2426
Size: 184 3571
MscI TGG'CCA
Cuts at: 1516 1516
Size: 3755
MslI CAynn'nnrTG
Cuts at: 1744 1871 1744
Size: 127 3628
MspA1I CmG'CkG
Cuts at: 173 336 669 866 1180 1540 2553 2798 173
Size: 163 333 197 314 360 1013 245 1130
Fragments arranged by size:
1130 1013 360 333 314 245 197 163
NaeI GCC'GGC
Cuts at: 1936 1936
Size: 3755
NarI GG'CG_CC
Cuts at: 1186 1434 1186
Size: 248 3507
NcoI C'CATG_G
Cuts at: 107 701 107
Size: 594 3161
NgoMIV G'CCGG_C
Cuts at: 1934 1934
Size: 3755
NheI G'CTAG_C
Cuts at: 472 472
Size: 3755
NspI r_CATG'y
Cuts at: 600 808 1308 2215 600
Size: 208 500 907 2140
Fragments arranged by size:
2140 907 500 208
PciI A'CATG_T
Cuts at: 596 2211 596
Size: 1615 2140
PmeI GTTT'AAAC
Cuts at: 754 754
Size: 3755
PmlI CAC'GTG
Cuts at: 621 621
Size: 3755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 3723
PpuMI rG'GwC_Cy
Cuts at: 127 127
Size: 3755
PspOMI G'GGCC_C
Cuts at: 115 115
Size: 3755
PstI C_TGCA'G
Cuts at: 802 1487 802
Size: 685 3070
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3755
PvuII CAG'CTG
Cuts at: 336 1180 1540 336
Size: 844 360 2551
Fragments arranged by size:
2551 844 360
RsrII CG'GwC_CG
Cuts at: 1950 1950
Size: 3755
SacI G_AGCT'C
Cuts at: 547 769 547
Size: 222 3533
SalI G'TCGA_C
Cuts at: 792 792
Size: 3755
SanDI GG'GwC_CC
Cuts at: 127 127
Size: 3755
SapI GCTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
SbfI CC_TGCA'GG
Cuts at: 802 802
Size: 3755
ScaI AGT'ACT
Cuts at: 3272 3272
Size: 3755
SfcI C'TryA_G
Cuts at: 33 798 1483 2476 2667 33
Size: 765 685 993 191 1121
Fragments arranged by size:
1121 993 765 685 191
SfoI GGC'GCC
Cuts at: 1187 1435 1187
Size: 248 3507
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
SmaI CCC'GGG
Cuts at: 777 777
Size: 3755
SmlI C'TyrA_G
Cuts at: 304 910 1825 2317 2579 304
Size: 606 915 492 262 1480
Fragments arranged by size:
1480 915 606 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3755
SphI G_CATG'C
Cuts at: 808 1308 808
Size: 500 3255
StuI AGG'CCT
Cuts at: 199 199
Size: 3755
StyI C'CwwG_G
Cuts at: 107 548 701 889 1243 107
Size: 441 153 188 354 2619
Fragments arranged by size:
2619 441 354 188 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 1471 1930 2139 1338
Size: 133 459 209 2954
Fragments arranged by size:
2954 459 209 133
TatI w'GTAC_w
Cuts at: 191 3270 191
Size: 3079 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 3723
Tth111I GACn'n_nGTC
Cuts at: 1552 1552
Size: 3755
XbaI T'CTAG_A
Cuts at: 786 786
Size: 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1250 1250
Size: 3755
XmaI C'CCGG_G
Cuts at: 775 775
Size: 3755
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BpmI Bpu10I BsaXI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PflMI PfoI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI