Restriction map for
pF1KE0637
(Circular) (Six-base) MAPSORT of: pF1KE0637.seq Check: 2637 from: 1 to: 3740
pF1KE0637 3740 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 778 778
Size: 3740
Acc65I G'GTAC_C
Cuts at: 683 756 683
Size: 73 3667
AclI AA'CG_TT
Cuts at: 3580 3580
Size: 3740
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 1565 1997 2744 3012 139
Size: 1426 432 747 268 867
Fragments arranged by size:
1426 867 747 432 268
AflIII A'CryG_T
Cuts at: 1092 2196 3173 1092
Size: 1104 977 1659
Fragments arranged by size:
1659 1104 977
AgeI A'CCGG_T
Cuts at: 2128 2128
Size: 3740
AlwNI CAG_nnn'CTG
Cuts at: 408 1045 2612 408
Size: 637 1567 1536
Fragments arranged by size:
1567 1536 637
ApaI G_GGCC'C
Cuts at: 576 576
Size: 3740
ApaLI G'TGCA_C
Cuts at: 484 2510 484
Size: 2026 1714
ApoI r'AATT_y
Cuts at: 744 3093 3349 744
Size: 2349 256 1135
Fragments arranged by size:
2349 1135 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3740
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
AvaI C'yCGr_G
Cuts at: 760 760
Size: 3740
AvrII C'CTAG_G
Cuts at: 495 495
Size: 3740
BamHI G'GATC_C
Cuts at: 765 765
Size: 3740
BanI G'GyrC_C
Cuts at: 151 178 683 756 1170 1418 1453 151
Size: 27 505 73 414 248 35 2438
Fragments arranged by size:
2438 505 414 248 73 35 27
BanII G_rGCy'C
Cuts at: 172 576 754 1784 172
Size: 404 178 1030 2128
Fragments arranged by size:
2128 1030 404 178
BbeI G_GCGC'C
Cuts at: 182 1174 1422 182
Size: 992 248 2500
Fragments arranged by size:
2500 992 248
BciVI GTATCCnnnnn_n'
Cuts at: 1638 2399 3740 1638
Size: 761 1341 1638
Fragments arranged by size:
1638 1341 761
BclI T'GATC_A
Cuts at: 290 1260 290
Size: 970 2770
BglII A'GATC_T
Cuts at: 1255 3292 1255
Size: 2037 1703
BlpI GC'TnA_GC
Cuts at: 852 852
Size: 3740
Bme1580I G_kGCm'C
Cuts at: 488 576 1365 1458 2514 488
Size: 88 789 93 1056 1714
Fragments arranged by size:
1714 1056 789 93 88
BmrI ACTGGGnnnn_n'
Cuts at: 952 1033 1368 3512 952
Size: 81 335 2144 1180
Fragments arranged by size:
2144 1180 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 3740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 205
Size: 3740
Bpu10I CC'TnA_GC
Cuts at: 671 671
Size: 3740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1795 2287 2585 916
Size: 879 492 298 2071
Fragments arranged by size:
2071 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3444 3444
Size: 3740
BsaAI yAC'GTr
Cuts at: 1093 1093
Size: 3740
BsaBI GATnn'nnATC
Cuts at: 6 1254 3291 6
Size: 1248 2037 455
Fragments arranged by size:
2037 1248 455
BsaHI Gr'CG_yC
Cuts at: 179 1171 1419 2121 3617 179
Size: 992 248 702 1496 302
Fragments arranged by size:
1496 992 702 302 248
BsaWI w'CCGG_w
Cuts at: 197 929 1450 2128 2402 2549 2960 197
Size: 732 521 678 274 147 411 977
Fragments arranged by size:
977 732 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 205 202
Size: 3 3737
BseYI C'CCAG_C
Cuts at: 475 606 704 1165 1923 2500 2996 475
Size: 131 98 461 758 577 496 1219
Fragments arranged by size:
1219 758 577 496 461 131 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 362
Size: 3740
BsiEI CG_ry'CG
Cuts at: 104 265 1282 1328 2536 104
Size: 161 1017 46 1208 1308
Fragments arranged by size:
1308 1208 1017 161 46
BsiHKAI G_wGCw'C
Cuts at: 137 488 504 754 1532 1722 2514 137
Size: 351 16 250 778 190 792 1363
Fragments arranged by size:
1363 792 778 351 250 190 16
BsmI GAATG_Cn'
Cuts at: 369 369
Size: 3740
Bsp1286I G_dGCh'C
Cuts at: 137 172 488 504 576 754 1365 1458 1532
Size: 35 316 16 72 178 611 93 74
Cuts at: 1532 1722 1784 2514 137
Size: 190 62 730 1363
Fragments arranged by size:
1363 730 611 316 190 178 93 74
72 62 35 16
BspEI T'CCGG_A
Cuts at: 197 2960 197
Size: 2763 977
BspHI T'CATG_A
Cuts at: 2916 2916
Size: 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 790 1004 1306 1687 790
Size: 214 302 381 2843
Fragments arranged by size:
2843 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2032 2086 3570 3735 50
Size: 1982 54 1484 165 55
Fragments arranged by size:
1982 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1652 1652
Size: 3740
BsrFI r'CCGG_y
Cuts at: 148 154 1738 1919 2128 148
Size: 6 1584 181 209 1760
Fragments arranged by size:
1760 1584 209 181 6
BsrGI T'GTAC_A
Cuts at: 587 587
Size: 3740
BssSI C'ACGA_G
Cuts at: 2011 2369 2011
Size: 358 3382
BstAPI GCAn_nnn'nTGC
Cuts at: 1117 1117
Size: 3740
BstBI TT'CG_AA
Cuts at: 2101 2101
Size: 3740
BstXI CCAn_nnnn'nTGG
Cuts at: 569 569
Size: 3740
BstYI r'GATC_y
Cuts at: 1 765 1232 1255 1590 1836 2848 2934 2946
Size: 764 467 23 335 246 1012 86 12
Cuts at: 2946 3292 1
Size: 346 449
Fragments arranged by size:
1012 764 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 495 1690 1871 2034 495
Size: 1195 181 163 2201
Fragments arranged by size:
2201 1195 181 163
BtsI GCAGTG_nn'
Cuts at: 299 644 983 299
Size: 345 339 3056
Fragments arranged by size:
3056 345 339
DraI TTT'AAA
Cuts at: 739 739
Size: 3740
DrdI GACnn_nn'nnGTC
Cuts at: 1446 2304 1446
Size: 858 2882
EaeI y'GGCC_r
Cuts at: 262 356 443 1325 1499 1890 1917 3262 262
Size: 94 87 882 174 391 27 1345 740
Fragments arranged by size:
1345 882 740 391 174 94 87 27
EagI C'GGCC_G
Cuts at: 262 1325 262
Size: 1063 2677
EarI CTCTTCn'nnn_
Cuts at: 262 1763 1973 262
Size: 1501 210 2029
Fragments arranged by size:
2029 1501 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1948 2258 2404 3550 1948
Size: 310 146 1146 2138
Fragments arranged by size:
2138 1146 310 146
EcoICRI GAG'CTC
Cuts at: 752 752
Size: 3740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 205 1565 1997 2744 3012 139
Size: 66 1360 432 747 268 867
Fragments arranged by size:
1360 867 747 432 268 66
EcoNI CCTnn'n_nnAGG
Cuts at: 187 187
Size: 3740
EcoO109I rG'GnC_Cy
Cuts at: 284 519 573 879 284
Size: 235 54 306 3145
Fragments arranged by size:
3145 306 235 54
EcoRI G'AATT_C
Cuts at: 744 744
Size: 3740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1759 1759
Size: 3740
FspI TGC'GCA
Cuts at: 1521 1521
Size: 3740
HaeII r_GCGC'y
Cuts at: 182 1174 1422 2444 3423 182
Size: 992 248 1022 979 499
Fragments arranged by size:
1022 992 979 499 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1582 1612 1614 1644 1582
Size: 30 2 30 3678
Fragments arranged by size:
3678 30 30 2
HincII GTy'rAC
Cuts at: 779 779
Size: 3740
KasI G'GCGC_C
Cuts at: 178 1170 1418 178
Size: 992 248 2500
Fragments arranged by size:
2500 992 248
KpnI G_GTAC'C
Cuts at: 687 760 687
Size: 73 3667
MluI A'CGCG_T
Cuts at: 3173 3173
Size: 3740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2411 2595 2411
Size: 184 3556
MscI TGG'CCA
Cuts at: 445 1501 445
Size: 1056 2684
MslI CAynn'nnrTG
Cuts at: 439 1729 1856 439
Size: 1290 127 2323
Fragments arranged by size:
2323 1290 127
MspA1I CmG'CkG
Cuts at: 215 652 851 1165 1525 2538 2783 215
Size: 437 199 314 360 1013 245 1172
Fragments arranged by size:
1172 1013 437 360 314 245 199
NaeI GCC'GGC
Cuts at: 1921 1921
Size: 3740
NarI GG'CG_CC
Cuts at: 179 1171 1419 179
Size: 992 248 2500
Fragments arranged by size:
2500 992 248
NgoMIV G'CCGG_C
Cuts at: 1919 1919
Size: 3740
NspI r_CATG'y
Cuts at: 793 1293 2200 793
Size: 500 907 2333
Fragments arranged by size:
2333 907 500
PciI A'CATG_T
Cuts at: 2196 2196
Size: 3740
PmeI GTTT'AAAC
Cuts at: 739 739
Size: 3740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 3708
PpuMI rG'GwC_Cy
Cuts at: 284 519 284
Size: 235 3505
PspOMI G'GGCC_C
Cuts at: 572 572
Size: 3740
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 712 744 712
Size: 32 3708
PstI C_TGCA'G
Cuts at: 787 1472 787
Size: 685 3055
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3740
PvuII CAG'CTG
Cuts at: 1165 1525 1165
Size: 360 3380
RsrII CG'GwC_CG
Cuts at: 1935 1935
Size: 3740
SacI G_AGCT'C
Cuts at: 754 754
Size: 3740
SalI G'TCGA_C
Cuts at: 777 777
Size: 3740
SanDI GG'GwC_CC
Cuts at: 284 519 284
Size: 235 3505
SapI GCTCTTCn'nnn_
Cuts at: 262 1763 1973 262
Size: 1501 210 2029
Fragments arranged by size:
2029 1501 210
SbfI CC_TGCA'GG
Cuts at: 787 787
Size: 3740
ScaI AGT'ACT
Cuts at: 3257 3257
Size: 3740
SfcI C'TryA_G
Cuts at: 33 783 1468 2461 2652 33
Size: 750 685 993 191 1121
Fragments arranged by size:
1121 993 750 685 191
SfoI GGC'GCC
Cuts at: 180 1172 1420 180
Size: 992 248 2500
Fragments arranged by size:
2500 992 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
SmaI CCC'GGG
Cuts at: 762 762
Size: 3740
SmlI C'TyrA_G
Cuts at: 895 1810 2302 2564 895
Size: 915 492 262 2071
Fragments arranged by size:
2071 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3740
SphI G_CATG'C
Cuts at: 793 1293 793
Size: 500 3240
StuI AGG'CCT
Cuts at: 568 568
Size: 3740
StyI C'CwwG_G
Cuts at: 495 874 1228 495
Size: 379 354 3007
Fragments arranged by size:
3007 379 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 718 1323 1456 1915 2124 718
Size: 605 133 459 209 2334
Fragments arranged by size:
2334 605 459 209 133
TatI w'GTAC_w
Cuts at: 587 3255 587
Size: 2668 1072
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 3708
Tth111I GACn'n_nGTC
Cuts at: 1537 1537
Size: 3740
XbaI T'CTAG_A
Cuts at: 771 771
Size: 3740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 438 1235 438
Size: 797 2943
XmaI C'CCGG_G
Cuts at: 760 760
Size: 3740
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspXI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI