Restriction map for
pF1KE0636
(Circular) (Six-base) MAPSORT of: pF1KE0636.seq Check: 282 from: 1 to: 4247
pF1KE0636 4247 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 1285 1285
Size: 4247
Acc65I G'GTAC_C
Cuts at: 1263 1263
Size: 4247
AclI AA'CG_TT
Cuts at: 4087 4087
Size: 4247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 930 2072 2504 3251 3519 930
Size: 1142 432 747 268 1658
Fragments arranged by size:
1658 1142 747 432 268
AflIII A'CryG_T
Cuts at: 888 1599 2703 3680 888
Size: 711 1104 977 1455
Fragments arranged by size:
1455 1104 977 711
AgeI A'CCGG_T
Cuts at: 2635 2635
Size: 4247
AleI CACnn'nnGTG
Cuts at: 1042 1042
Size: 4247
AlwNI CAG_nnn'CTG
Cuts at: 349 1552 3119 349
Size: 1203 1567 1477
Fragments arranged by size:
1567 1477 1203
ApaLI G'TGCA_C
Cuts at: 3017 3017
Size: 4247
ApoI r'AATT_y
Cuts at: 434 728 1064 1232 1251 3600 3856 434
Size: 294 336 168 19 2349 256 825
Fragments arranged by size:
2349 825 336 294 256 168 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 4247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
AvaI C'yCGr_G
Cuts at: 1267 1267
Size: 4247
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 993 1026 993
Size: 33 4214
BamHI G'GATC_C
Cuts at: 1272 1272
Size: 4247
BanI G'GyrC_C
Cuts at: 1263 1677 1925 1960 1263
Size: 414 248 35 3550
Fragments arranged by size:
3550 414 248 35
BanII G_rGCy'C
Cuts at: 340 1261 2291 340
Size: 921 1030 2296
Fragments arranged by size:
2296 1030 921
BbeI G_GCGC'C
Cuts at: 1681 1929 1681
Size: 248 3999
BciVI GTATCCnnnnn_n'
Cuts at: 2145 2906 4247 2145
Size: 761 1341 2145
Fragments arranged by size:
2145 1341 761
BclI T'GATC_A
Cuts at: 110 1767 110
Size: 1657 2590
BfrBI ATG'CAT
Cuts at: 783 783
Size: 4247
BglII A'GATC_T
Cuts at: 1762 3799 1762
Size: 2037 2210
BlpI GC'TnA_GC
Cuts at: 1359 1359
Size: 4247
Bme1580I G_kGCm'C
Cuts at: 1872 1965 3021 1872
Size: 93 1056 3098
Fragments arranged by size:
3098 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1459 1540 1875 4019 1459
Size: 81 335 2144 1687
Fragments arranged by size:
2144 1687 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 4247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 842 326
Size: 516 3731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 928 1423 2302 2794 3092 265
Size: 663 495 879 492 298 1420
Fragments arranged by size:
1420 879 663 495 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 738 3951 738
Size: 3213 1034
BsaAI yAC'GTr
Cuts at: 891 1600 891
Size: 709 3538
BsaBI GATnn'nnATC
Cuts at: 6 1761 3798 6
Size: 1755 2037 455
Fragments arranged by size:
2037 1755 455
BsaHI Gr'CG_yC
Cuts at: 1678 1926 2628 4124 1678
Size: 248 702 1496 1801
Fragments arranged by size:
1801 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1436 1957 2635 2909 3056 3467 1436
Size: 521 678 274 147 411 2216
Fragments arranged by size:
2216 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 864 1123 181
Size: 683 259 3305
Fragments arranged by size:
3305 683 259
BseYI C'CCAG_C
Cuts at: 1672 2430 3007 3503 1672
Size: 758 577 496 2416
Fragments arranged by size:
2416 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 861 861
Size: 4247
BsiEI CG_ry'CG
Cuts at: 104 1789 1835 3043 104
Size: 1685 46 1208 1308
Fragments arranged by size:
1685 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 340 475 1261 2039 2229 3021 340
Size: 135 786 778 190 792 1566
Fragments arranged by size:
1566 792 786 778 190 135
BsmI GAATG_Cn'
Cuts at: 592 601 676 592
Size: 9 75 4163
Fragments arranged by size:
4163 75 9
Bsp1286I G_dGCh'C
Cuts at: 340 475 1261 1872 1965 2039 2229 2291 3021
Size: 135 786 611 93 74 190 62 730
Cuts at: 3021 340
Size: 1566
Fragments arranged by size:
1566 786 730 611 190 135 93 74
62
BspEI T'CCGG_A
Cuts at: 3467 3467
Size: 4247
BspHI T'CATG_A
Cuts at: 3423 3423
Size: 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1297 1511 1813 2194 1297
Size: 214 302 381 3350
Fragments arranged by size:
3350 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2539 2593 4077 4242 50
Size: 2489 54 1484 165 55
Fragments arranged by size:
2489 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 681 765 2159 681
Size: 84 1394 2769
Fragments arranged by size:
2769 1394 84
BsrFI r'CCGG_y
Cuts at: 2245 2426 2635 2245
Size: 181 209 3857
Fragments arranged by size:
3857 209 181
BsrGI T'GTAC_A
Cuts at: 1221 1221
Size: 4247
BssSI C'ACGA_G
Cuts at: 331 796 2518 2876 331
Size: 465 1722 358 1702
Fragments arranged by size:
1722 1702 465 358
BstAPI GCAn_nnn'nTGC
Cuts at: 596 712 1624 596
Size: 116 912 3219
Fragments arranged by size:
3219 912 116
BstBI TT'CG_AA
Cuts at: 2608 2608
Size: 4247
BstYI r'GATC_y
Cuts at: 1 1272 1739 1762 2097 2343 3355 3441 3453
Size: 1271 467 23 335 246 1012 86 12
Cuts at: 3453 3799 1
Size: 346 449
Fragments arranged by size:
1271 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 304 304
Size: 4247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2197 2378 2541 2197
Size: 181 163 3903
Fragments arranged by size:
3903 181 163
BtsI GCAGTG_nn'
Cuts at: 158 597 1490 158
Size: 439 893 2915
Fragments arranged by size:
2915 893 439
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 663 698 747 782 663
Size: 35 49 35 4128
Fragments arranged by size:
4128 49 35 35
DraI TTT'AAA
Cuts at: 1246 1246
Size: 4247
DrdI GACnn_nn'nnGTC
Cuts at: 1953 2811 1953
Size: 858 3389
EaeI y'GGCC_r
Cuts at: 1832 2006 2397 2424 3769 1832
Size: 174 391 27 1345 2310
Fragments arranged by size:
2310 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1832 1832
Size: 4247
EarI CTCTTCn'nnn_
Cuts at: 2270 2480 2270
Size: 210 4037
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2455 2765 2911 4057 2455
Size: 310 146 1146 2645
Fragments arranged by size:
2645 1146 310 146
EcoICRI GAG'CTC
Cuts at: 338 1259 338
Size: 921 3326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 842 930 2072 2504 3251 3519 326
Size: 516 88 1142 432 747 268 1054
Fragments arranged by size:
1142 1054 747 516 432 268 88
EcoNI CCTnn'n_nnAGG
Cuts at: 696 696
Size: 4247
EcoO109I rG'GnC_Cy
Cuts at: 1386 1386
Size: 4247
EcoRI G'AATT_C
Cuts at: 1064 1251 1064
Size: 187 4060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 4247
FspI TGC'GCA
Cuts at: 2028 2028
Size: 4247
HaeII r_GCGC'y
Cuts at: 1681 1929 2951 3930 1681
Size: 248 1022 979 1998
Fragments arranged by size:
1998 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 462 494 2089 2119 2121 2151 462
Size: 32 1595 30 2 30 2558
Fragments arranged by size:
2558 1595 32 30 30 2
HincII GTy'rAC
Cuts at: 1286 1286
Size: 4247
HindIII A'AGCT_T
Cuts at: 1025 1025
Size: 4247
KasI G'GCGC_C
Cuts at: 1677 1925 1677
Size: 248 3999
KpnI G_GTAC'C
Cuts at: 1267 1267
Size: 4247
MluI A'CGCG_T
Cuts at: 3680 3680
Size: 4247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 2918 3102 200
Size: 2718 184 1345
Fragments arranged by size:
2718 1345 184
MscI TGG'CCA
Cuts at: 2008 2008
Size: 4247
MslI CAynn'nnrTG
Cuts at: 244 921 1042 2236 2363 244
Size: 677 121 1194 127 2128
Fragments arranged by size:
2128 1194 677 127 121
MspA1I CmG'CkG
Cuts at: 177 1358 1672 2032 3045 3290 177
Size: 1181 314 360 1013 245 1134
Fragments arranged by size:
1181 1134 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2428 2428
Size: 4247
NarI GG'CG_CC
Cuts at: 1678 1926 1678
Size: 248 3999
NcoI C'CATG_G
Cuts at: 304 304
Size: 4247
NdeI CA'TA_TG
Cuts at: 1015 1015
Size: 4247
NgoMIV G'CCGG_C
Cuts at: 2426 2426
Size: 4247
NsiI A_TGCA'T
Cuts at: 785 785
Size: 4247
NspI r_CATG'y
Cuts at: 1300 1800 2707 1300
Size: 500 907 2840
Fragments arranged by size:
2840 907 500
PciI A'CATG_T
Cuts at: 2703 2703
Size: 4247
PflMI CCAn_nnn'nTGG
Cuts at: 144 798 144
Size: 654 3593
PfoI T'CCnGG_A
Cuts at: 119 137 119
Size: 18 4229
PmeI GTTT'AAAC
Cuts at: 1246 1246
Size: 4247
PmlI CAC'GTG
Cuts at: 891 891
Size: 4247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 4215
PsiI TTA'TAA
Cuts at: 464 968 464
Size: 504 3743
PstI C_TGCA'G
Cuts at: 165 1294 1979 165
Size: 1129 685 2433
Fragments arranged by size:
2433 1129 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4247
PvuII CAG'CTG
Cuts at: 177 1672 2032 177
Size: 1495 360 2392
Fragments arranged by size:
2392 1495 360
RsrII CG'GwC_CG
Cuts at: 2442 2442
Size: 4247
SacI G_AGCT'C
Cuts at: 340 1261 340
Size: 921 3326
SalI G'TCGA_C
Cuts at: 1284 1284
Size: 4247
SapI GCTCTTCn'nnn_
Cuts at: 2270 2480 2270
Size: 210 4037
SbfI CC_TGCA'GG
Cuts at: 1294 1294
Size: 4247
ScaI AGT'ACT
Cuts at: 3764 3764
Size: 4247
SfcI C'TryA_G
Cuts at: 33 161 228 569 693 1290 1975 2968 3159
Size: 128 67 341 124 597 685 993 191
Cuts at: 3159 33
Size: 1121
Fragments arranged by size:
1121 993 685 597 341 191 128 124
67
SfoI GGC'GCC
Cuts at: 1679 1927 1679
Size: 248 3999
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
SmaI CCC'GGG
Cuts at: 1269 1269
Size: 4247
SmlI C'TyrA_G
Cuts at: 280 907 1402 2317 2809 3071 280
Size: 627 495 915 492 262 1456
Fragments arranged by size:
1456 915 627 495 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4247
SphI G_CATG'C
Cuts at: 1300 1800 1300
Size: 500 3747
StyI C'CwwG_G
Cuts at: 304 1381 1735 304
Size: 1077 354 2816
Fragments arranged by size:
2816 1077 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1830 1963 2422 2631 1830
Size: 133 459 209 3446
Fragments arranged by size:
3446 459 209 133
TatI w'GTAC_w
Cuts at: 1221 3762 1221
Size: 2541 1706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4215
Tth111I GACn'n_nGTC
Cuts at: 2044 2044
Size: 4247
XbaI T'CTAG_A
Cuts at: 1278 1278
Size: 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1742 1742
Size: 4247
XmaI C'CCGG_G
Cuts at: 1267 1267
Size: 4247
XmnI GAAnn'nnTTC
Cuts at: 607 859 943 607
Size: 252 84 3911
Fragments arranged by size:
3911 252 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI PacI PasI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI