Restriction map for
pF1KE0633
(Circular) (Six-base) MAPSORT of: pF1KE0633.seq Check: 5214 from: 1 to: 3719
pF1KE0633 3719 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 757 757
Size: 3719
Acc65I G'GTAC_C
Cuts at: 735 735
Size: 3719
AclI AA'CG_TT
Cuts at: 3559 3559
Size: 3719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1976 2723 2991 1544
Size: 432 747 268 2272
Fragments arranged by size:
2272 747 432 268
AflIII A'CryG_T
Cuts at: 1071 2175 3152 1071
Size: 1104 977 1638
Fragments arranged by size:
1638 1104 977
AgeI A'CCGG_T
Cuts at: 200 2107 200
Size: 1907 1812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 462 462
Size: 3719
AlwNI CAG_nnn'CTG
Cuts at: 1024 2591 1024
Size: 1567 2152
ApaLI G'TGCA_C
Cuts at: 409 2489 409
Size: 2080 1639
ApoI r'AATT_y
Cuts at: 723 3072 3328 723
Size: 2349 256 1114
Fragments arranged by size:
2349 1114 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
AvaI C'yCGr_G
Cuts at: 424 518 544 699 739 424
Size: 94 26 155 40 3404
Fragments arranged by size:
3404 155 94 40 26
BamHI G'GATC_C
Cuts at: 744 744
Size: 3719
BanI G'GyrC_C
Cuts at: 735 1149 1397 1432 735
Size: 414 248 35 3022
Fragments arranged by size:
3022 414 248 35
BanII G_rGCy'C
Cuts at: 706 733 1763 706
Size: 27 1030 2662
Fragments arranged by size:
2662 1030 27
BbeI G_GCGC'C
Cuts at: 1153 1401 1153
Size: 248 3471
BciVI GTATCCnnnnn_n'
Cuts at: 1617 2378 3719 1617
Size: 761 1341 1617
Fragments arranged by size:
1617 1341 761
BclI T'GATC_A
Cuts at: 572 1239 572
Size: 667 3052
BfrBI ATG'CAT
Cuts at: 449 449
Size: 3719
BglII A'GATC_T
Cuts at: 1234 3271 1234
Size: 2037 1682
BlpI GC'TnA_GC
Cuts at: 831 831
Size: 3719
Bme1580I G_kGCm'C
Cuts at: 413 1344 1437 2493 413
Size: 931 93 1056 1639
Fragments arranged by size:
1639 1056 931 93
BmrI ACTGGGnnnn_n'
Cuts at: 931 1012 1347 3491 931
Size: 81 335 2144 1159
Fragments arranged by size:
2144 1159 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 3719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 459
Size: 3719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1774 2266 2564 895
Size: 879 492 298 2050
Fragments arranged by size:
2050 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3423 3423
Size: 3719
BsaAI yAC'GTr
Cuts at: 1072 1072
Size: 3719
BsaBI GATnn'nnATC
Cuts at: 6 1233 3270 6
Size: 1227 2037 455
Fragments arranged by size:
2037 1227 455
BsaHI Gr'CG_yC
Cuts at: 1150 1398 2100 3596 1150
Size: 248 702 1496 1273
Fragments arranged by size:
1496 1273 702 248
BsaWI w'CCGG_w
Cuts at: 200 341 908 1429 2107 2381 2528 2939 200
Size: 141 567 521 678 274 147 411 980
Fragments arranged by size:
980 678 567 521 411 274 147 141
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 190 220 190
Size: 30 3689
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 645 364
Size: 281 3438
BseYI C'CCAG_C
Cuts at: 1144 1902 2479 2975 1144
Size: 758 577 496 1888
Fragments arranged by size:
1888 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1261 1307 2515 104
Size: 1157 46 1208 1308
Fragments arranged by size:
1308 1208 1157 46
BsiHKAI G_wGCw'C
Cuts at: 208 413 733 1511 1701 2493 208
Size: 205 320 778 190 792 1434
Fragments arranged by size:
1434 792 778 320 205 190
Bsp1286I G_dGCh'C
Cuts at: 208 413 706 733 1344 1437 1511 1701 1763
Size: 205 293 27 611 93 74 190 62
Cuts at: 1763 2493 208
Size: 730 1434
Fragments arranged by size:
1434 730 611 293 205 190 93 74
62 27
BspEI T'CCGG_A
Cuts at: 341 2939 341
Size: 2598 1121
BspHI T'CATG_A
Cuts at: 2895 2895
Size: 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 499 769 983 1285 1666 499
Size: 270 214 302 381 2552
Fragments arranged by size:
2552 381 302 270 214
BsrBI CCG'CTC
Cuts at: 50 363 2011 2065 3549 3714 50
Size: 313 1648 54 1484 165 55
Fragments arranged by size:
1648 1484 313 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1631 1631
Size: 3719
BsrFI r'CCGG_y
Cuts at: 188 200 1717 1898 2107 188
Size: 12 1517 181 209 1800
Fragments arranged by size:
1800 1517 209 181 12
BssSI C'ACGA_G
Cuts at: 316 1990 2348 316
Size: 1674 358 1687
Fragments arranged by size:
1687 1674 358
BstAPI GCAn_nnn'nTGC
Cuts at: 408 1096 408
Size: 688 3031
BstBI TT'CG_AA
Cuts at: 2080 2080
Size: 3719
BstEII G'GTnAC_C
Cuts at: 511 511
Size: 3719
BstYI r'GATC_y
Cuts at: 1 744 1211 1234 1569 1815 2827 2913 2925
Size: 743 467 23 335 246 1012 86 12
Cuts at: 2925 3271 1
Size: 346 449
Fragments arranged by size:
1012 743 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 368 107
Size: 261 3458
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1669 1850 2013 1669
Size: 181 163 3375
Fragments arranged by size:
3375 181 163
BtsI GCAGTG_nn'
Cuts at: 962 962
Size: 3719
DraI TTT'AAA
Cuts at: 718 718
Size: 3719
DraIII CAC_nnn'GTG
Cuts at: 318 318
Size: 3719
DrdI GACnn_nn'nnGTC
Cuts at: 1425 2283 1425
Size: 858 2861
EaeI y'GGCC_r
Cuts at: 533 584 1304 1478 1869 1896 3241 533
Size: 51 720 174 391 27 1345 1011
Fragments arranged by size:
1345 1011 720 391 174 51 27
EagI C'GGCC_G
Cuts at: 1304 1304
Size: 3719
EarI CTCTTCn'nnn_
Cuts at: 275 1742 1952 275
Size: 1467 210 2042
Fragments arranged by size:
2042 1467 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1927 2237 2383 3529 1927
Size: 310 146 1146 2117
Fragments arranged by size:
2117 1146 310 146
EcoICRI GAG'CTC
Cuts at: 731 731
Size: 3719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 1544 1976 2723 2991 459
Size: 1085 432 747 268 1187
Fragments arranged by size:
1187 1085 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 498 597 648 858 498
Size: 99 51 210 3359
Fragments arranged by size:
3359 210 99 51
EcoRI G'AATT_C
Cuts at: 723 723
Size: 3719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1738 1738
Size: 3719
FspI TGC'GCA
Cuts at: 1500 1500
Size: 3719
HaeII r_GCGC'y
Cuts at: 1153 1401 2423 3402 1153
Size: 248 1022 979 1470
Fragments arranged by size:
1470 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 542 574 640 672 1561 1591 1593
Size: 32 320 32 66 32 889 30 2
Cuts at: 1593 1623 190
Size: 30 2286
Fragments arranged by size:
2286 889 320 66 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 156 758 156
Size: 602 3117
HpaI GTT'AAC
Cuts at: 156 156
Size: 3719
KasI G'GCGC_C
Cuts at: 1149 1397 1149
Size: 248 3471
KpnI G_GTAC'C
Cuts at: 739 739
Size: 3719
MluI A'CGCG_T
Cuts at: 3152 3152
Size: 3719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2390 2574 2390
Size: 184 3535
MscI TGG'CCA
Cuts at: 535 1480 535
Size: 945 2774
MslI CAynn'nnrTG
Cuts at: 416 580 1708 1835 416
Size: 164 1128 127 2300
Fragments arranged by size:
2300 1128 164 127
MspA1I CmG'CkG
Cuts at: 389 830 1144 1504 2517 2762 389
Size: 441 314 360 1013 245 1346
Fragments arranged by size:
1346 1013 441 360 314 245
NaeI GCC'GGC
Cuts at: 190 1900 190
Size: 1710 2009
NarI GG'CG_CC
Cuts at: 1150 1398 1150
Size: 248 3471
NcoI C'CATG_G
Cuts at: 107 107
Size: 3719
NgoMIV G'CCGG_C
Cuts at: 188 1898 188
Size: 1710 2009
NsiI A_TGCA'T
Cuts at: 451 451
Size: 3719
NspI r_CATG'y
Cuts at: 772 1272 2179 772
Size: 500 907 2312
Fragments arranged by size:
2312 907 500
PciI A'CATG_T
Cuts at: 2175 2175
Size: 3719
PflMI CCAn_nnn'nTGG
Cuts at: 318 318
Size: 3719
PmeI GTTT'AAAC
Cuts at: 718 718
Size: 3719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 3687
PpuMI rG'GwC_Cy
Cuts at: 498 648 498
Size: 150 3569
PstI C_TGCA'G
Cuts at: 260 766 1451 260
Size: 506 685 2528
Fragments arranged by size:
2528 685 506
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3719
PvuII CAG'CTG
Cuts at: 1144 1504 1144
Size: 360 3359
RsrII CG'GwC_CG
Cuts at: 1914 1914
Size: 3719
SacI G_AGCT'C
Cuts at: 733 733
Size: 3719
SalI G'TCGA_C
Cuts at: 756 756
Size: 3719
SanDI GG'GwC_CC
Cuts at: 498 498
Size: 3719
SapI GCTCTTCn'nnn_
Cuts at: 1742 1952 1742
Size: 210 3509
SbfI CC_TGCA'GG
Cuts at: 766 766
Size: 3719
ScaI AGT'ACT
Cuts at: 3236 3236
Size: 3719
SfcI C'TryA_G
Cuts at: 33 256 762 1447 2440 2631 33
Size: 223 506 685 993 191 1121
Fragments arranged by size:
1121 993 685 506 223 191
SfoI GGC'GCC
Cuts at: 1151 1399 1151
Size: 248 3471
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
SmaI CCC'GGG
Cuts at: 701 741 701
Size: 40 3679
SmlI C'TyrA_G
Cuts at: 874 1789 2281 2543 874
Size: 915 492 262 2050
Fragments arranged by size:
2050 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3719
SphI G_CATG'C
Cuts at: 772 1272 772
Size: 500 3219
SspI AAT'ATT
Cuts at: 118 118
Size: 3719
StyI C'CwwG_G
Cuts at: 107 303 853 1207 107
Size: 196 550 354 2619
Fragments arranged by size:
2619 550 354 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1302 1435 1894 2103 1302
Size: 133 459 209 2918
Fragments arranged by size:
2918 459 209 133
TatI w'GTAC_w
Cuts at: 3234 3234
Size: 3719
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 3687
Tth111I GACn'n_nGTC
Cuts at: 1516 1516
Size: 3719
XbaI T'CTAG_A
Cuts at: 750 750
Size: 3719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1214 1214
Size: 3719
XmaI C'CCGG_G
Cuts at: 699 739 699
Size: 40 3679
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NruI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI