Restriction map for
pF1KE0631
(Circular) (Six-base) MAPSORT of: pF1KE0631.seq Check: 263 from: 1 to: 3713
pF1KE0631 3713 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AatII G_ACGT'C
Cuts at: 665 665
Size: 3713
AccI GT'mk_AC
Cuts at: 751 751
Size: 3713
Acc65I G'GTAC_C
Cuts at: 729 729
Size: 3713
AclI AA'CG_TT
Cuts at: 3553 3553
Size: 3713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1538 1970 2717 2985 1538
Size: 432 747 268 2266
Fragments arranged by size:
2266 747 432 268
AflIII A'CryG_T
Cuts at: 242 1065 2169 3146 242
Size: 823 1104 977 809
Fragments arranged by size:
1104 977 823 809
AgeI A'CCGG_T
Cuts at: 264 2101 264
Size: 1837 1876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 340 545 340
Size: 205 3508
AlwNI CAG_nnn'CTG
Cuts at: 1018 2585 1018
Size: 1567 2146
ApaI G_GGCC'C
Cuts at: 188 285 491 188
Size: 97 206 3410
Fragments arranged by size:
3410 206 97
ApaLI G'TGCA_C
Cuts at: 508 2483 508
Size: 1975 1738
ApoI r'AATT_y
Cuts at: 581 717 3066 3322 581
Size: 136 2349 256 972
Fragments arranged by size:
2349 972 256 136
AseI AT'TA_AT
Cuts at: 20 20
Size: 3713
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
AvaI C'yCGr_G
Cuts at: 733 733
Size: 3713
BamHI G'GATC_C
Cuts at: 738 738
Size: 3713
BanI G'GyrC_C
Cuts at: 729 1143 1391 1426 729
Size: 414 248 35 3016
Fragments arranged by size:
3016 414 248 35
BanII G_rGCy'C
Cuts at: 188 285 491 727 1757 188
Size: 97 206 236 1030 2144
Fragments arranged by size:
2144 1030 236 206 97
BbeI G_GCGC'C
Cuts at: 1147 1395 1147
Size: 248 3465
BbsI GAAGACnn'nnnn_
Cuts at: 375 686 375
Size: 311 3402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 390 424 567 601 390
Size: 34 143 34 3502
Fragments arranged by size:
3502 143 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1611 2372 3713 1611
Size: 761 1341 1611
Fragments arranged by size:
1611 1341 761
BclI T'GATC_A
Cuts at: 1233 1233
Size: 3713
BglI GCCn_nnn'nGGC
Cuts at: 280 280
Size: 3713
BglII A'GATC_T
Cuts at: 1228 3265 1228
Size: 2037 1676
BlpI GC'TnA_GC
Cuts at: 825 825
Size: 3713
Bme1580I G_kGCm'C
Cuts at: 188 285 491 512 1338 1431 2487 188
Size: 97 206 21 826 93 1056 1414
Fragments arranged by size:
1414 1056 826 206 97 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 925 1006 1341 3485 925
Size: 81 335 2144 1153
Fragments arranged by size:
2144 1153 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 3713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 3713
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1768 2260 2558 889
Size: 879 492 298 2044
Fragments arranged by size:
2044 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 283 3417 283
Size: 3134 579
BsaAI yAC'GTr
Cuts at: 123 1066 123
Size: 943 2770
BsaBI GATnn'nnATC
Cuts at: 6 1227 3264 6
Size: 1221 2037 455
Fragments arranged by size:
2037 1221 455
BsaHI Gr'CG_yC
Cuts at: 272 662 1144 1392 2094 3590 272
Size: 390 482 248 702 1496 395
Fragments arranged by size:
1496 702 482 395 390 248
BsaWI w'CCGG_w
Cuts at: 264 902 1423 2101 2375 2522 2933 264
Size: 638 521 678 274 147 411 1044
Fragments arranged by size:
1044 678 638 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 442 472 442
Size: 30 3683
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 706 706
Size: 3713
BseYI C'CCAG_C
Cuts at: 181 1138 1896 2473 2969 181
Size: 957 758 577 496 925
Fragments arranged by size:
957 925 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 118
Size: 3713
BsiEI CG_ry'CG
Cuts at: 104 1255 1301 2509 104
Size: 1151 46 1208 1308
Fragments arranged by size:
1308 1208 1151 46
BsiHKAI G_wGCw'C
Cuts at: 512 727 1505 1695 2487 512
Size: 215 778 190 792 1738
Fragments arranged by size:
1738 792 778 215 190
Bsp1286I G_dGCh'C
Cuts at: 188 285 491 512 727 1338 1431 1505 1695
Size: 97 206 21 215 611 93 74 190
Cuts at: 1695 1757 2487 188
Size: 62 730 1414
Fragments arranged by size:
1414 730 611 215 206 190 97 93
74 62 21
BspEI T'CCGG_A
Cuts at: 2933 2933
Size: 3713
BspHI T'CATG_A
Cuts at: 2889 2889
Size: 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 140 763 977 1279 1660 140
Size: 623 214 302 381 2193
Fragments arranged by size:
2193 623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 230 368 537 2005 2059 3543 3708 50
Size: 180 138 169 1468 54 1484 165 55
Fragments arranged by size:
1484 1468 180 169 165 138 55 54
BsrDI GCAATG_nn'
Cuts at: 161 1625 161
Size: 1464 2249
BsrFI r'CCGG_y
Cuts at: 264 1711 1892 2101 264
Size: 1447 181 209 1876
Fragments arranged by size:
1876 1447 209 181
BsrGI T'GTAC_A
Cuts at: 425 425
Size: 3713
BssSI C'ACGA_G
Cuts at: 1984 2342 1984
Size: 358 3355
BstAPI GCAn_nnn'nTGC
Cuts at: 1090 1090
Size: 3713
BstBI TT'CG_AA
Cuts at: 2074 2074
Size: 3713
BstEII G'GTnAC_C
Cuts at: 144 144
Size: 3713
BstYI r'GATC_y
Cuts at: 1 738 1205 1228 1563 1809 2821 2907 2919
Size: 737 467 23 335 246 1012 86 12
Cuts at: 2919 3265 1
Size: 346 449
Fragments arranged by size:
1012 737 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 140 140
Size: 3713
BtgI C'CryG_G
Cuts at: 107 107
Size: 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1663 1844 2007 1663
Size: 181 163 3369
Fragments arranged by size:
3369 181 163
BtsI GCAGTG_nn'
Cuts at: 956 956
Size: 3713
ClaI AT'CG_AT
Cuts at: 545 545
Size: 3713
DraI TTT'AAA
Cuts at: 712 712
Size: 3713
DrdI GACnn_nn'nnGTC
Cuts at: 1419 2277 1419
Size: 858 2855
EaeI y'GGCC_r
Cuts at: 1298 1472 1863 1890 3235 1298
Size: 174 391 27 1345 1776
Fragments arranged by size:
1776 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1298 1298
Size: 3713
EarI CTCTTCn'nnn_
Cuts at: 1736 1946 1736
Size: 210 3503
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1921 2231 2377 3523 1921
Size: 310 146 1146 2111
Fragments arranged by size:
2111 1146 310 146
EcoICRI GAG'CTC
Cuts at: 725 725
Size: 3713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 1538 1970 2717 2985 307
Size: 1231 432 747 268 1035
Fragments arranged by size:
1231 1035 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 639 639
Size: 3713
EcoO109I rG'GnC_Cy
Cuts at: 282 348 487 852 282
Size: 66 139 365 3143
Fragments arranged by size:
3143 365 139 66
EcoRI G'AATT_C
Cuts at: 717 717
Size: 3713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1732 1732
Size: 3713
FspI TGC'GCA
Cuts at: 328 1494 328
Size: 1166 2547
FspAI rTGC'GCAy
Cuts at: 328 328
Size: 3713
HaeII r_GCGC'y
Cuts at: 424 1147 1395 2417 3396 424
Size: 723 248 1022 979 741
Fragments arranged by size:
1022 979 741 723 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1555 1585 1587 1617 1555
Size: 30 2 30 3651
Fragments arranged by size:
3651 30 30 2
HincII GTy'rAC
Cuts at: 252 752 252
Size: 500 3213
KasI G'GCGC_C
Cuts at: 1143 1391 1143
Size: 248 3465
KpnI G_GTAC'C
Cuts at: 733 733
Size: 3713
MluI A'CGCG_T
Cuts at: 242 3146 242
Size: 2904 809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2568 2384
Size: 184 3529
MscI TGG'CCA
Cuts at: 1474 1474
Size: 3713
MslI CAynn'nnrTG
Cuts at: 547 1702 1829 547
Size: 1155 127 2431
Fragments arranged by size:
2431 1155 127
MspA1I CmG'CkG
Cuts at: 181 824 1138 1498 2511 2756 181
Size: 643 314 360 1013 245 1138
Fragments arranged by size:
1138 1013 643 360 314 245
NaeI GCC'GGC
Cuts at: 1894 1894
Size: 3713
NarI GG'CG_CC
Cuts at: 1144 1392 1144
Size: 248 3465
NcoI C'CATG_G
Cuts at: 107 107
Size: 3713
NgoMIV G'CCGG_C
Cuts at: 1892 1892
Size: 3713
NspI r_CATG'y
Cuts at: 564 766 1266 2173 564
Size: 202 500 907 2104
Fragments arranged by size:
2104 907 500 202
PciI A'CATG_T
Cuts at: 2169 2169
Size: 3713
PmeI GTTT'AAAC
Cuts at: 712 712
Size: 3713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2852 2884 2852
Size: 32 3681
PpuMI rG'GwC_Cy
Cuts at: 348 348
Size: 3713
PspOMI G'GGCC_C
Cuts at: 184 281 487 184
Size: 97 206 3410
Fragments arranged by size:
3410 206 97
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 154 186 154
Size: 32 3681
PstI C_TGCA'G
Cuts at: 211 760 1445 211
Size: 549 685 2479
Fragments arranged by size:
2479 685 549
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3713
PvuII CAG'CTG
Cuts at: 181 1138 1498 181
Size: 957 360 2396
Fragments arranged by size:
2396 957 360
RsrII CG'GwC_CG
Cuts at: 1908 1908
Size: 3713
SacI G_AGCT'C
Cuts at: 727 727
Size: 3713
SalI G'TCGA_C
Cuts at: 750 750
Size: 3713
SanDI GG'GwC_CC
Cuts at: 348 348
Size: 3713
SapI GCTCTTCn'nnn_
Cuts at: 1736 1946 1736
Size: 210 3503
SbfI CC_TGCA'GG
Cuts at: 760 760
Size: 3713
ScaI AGT'ACT
Cuts at: 3230 3230
Size: 3713
SfcI C'TryA_G
Cuts at: 33 207 756 1441 2434 2625 33
Size: 174 549 685 993 191 1121
Fragments arranged by size:
1121 993 685 549 191 174
SfoI GGC'GCC
Cuts at: 1145 1393 1145
Size: 248 3465
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
SmaI CCC'GGG
Cuts at: 735 735
Size: 3713
SmlI C'TyrA_G
Cuts at: 868 1783 2275 2537 868
Size: 915 492 262 2044
Fragments arranged by size:
2044 915 492 262
SpeI A'CTAG_T
Cuts at: 67 652 67
Size: 585 3128
SphI G_CATG'C
Cuts at: 766 1266 766
Size: 500 3213
StyI C'CwwG_G
Cuts at: 107 491 847 1201 107
Size: 384 356 354 2619
Fragments arranged by size:
2619 384 356 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 1296 1429 1888 2097 163
Size: 1133 133 459 209 1779
Fragments arranged by size:
1779 1133 459 209 133
TatI w'GTAC_w
Cuts at: 425 3228 425
Size: 2803 910
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 3681
Tth111I GACn'n_nGTC
Cuts at: 1510 1510
Size: 3713
XbaI T'CTAG_A
Cuts at: 744 744
Size: 3713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 282 1208 282
Size: 926 2787
XmaI C'CCGG_G
Cuts at: 733 733
Size: 3713
ZraI GAC'GTC
Cuts at: 663 663
Size: 3713
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BssHII BstXI BstZ17I CspCI DraIII EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspXI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI