Restriction map for
pF1KE0630
(Circular) (Six-base) MAPSORT of: pF1KE0630.seq Check: 5126 from: 1 to: 4226
pF1KE0630 4226 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1264 1264
Size: 4226
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 175 4066 175
Size: 3891 335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2483 3230 3498 2051
Size: 432 747 268 2779
Fragments arranged by size:
2779 747 432 268
AflIII A'CryG_T
Cuts at: 348 1578 2682 3659 348
Size: 1230 1104 977 915
Fragments arranged by size:
1230 1104 977 915
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AleI CACnn'nnGTG
Cuts at: 217 217
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 594 1531 3098 594
Size: 937 1567 1722
Fragments arranged by size:
1722 1567 937
ApaI G_GGCC'C
Cuts at: 1146 1146
Size: 4226
ApaLI G'TGCA_C
Cuts at: 2996 2996
Size: 4226
ApoI r'AATT_y
Cuts at: 238 1230 3579 3835 238
Size: 992 2349 256 629
Fragments arranged by size:
2349 992 629 256
AscI GG'CGCG_CC
Cuts at: 376 376
Size: 4226
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 765 1037 1178 1246 765
Size: 272 141 68 3745
Fragments arranged by size:
3745 272 141 68
AvrII C'CTAG_G
Cuts at: 795 795
Size: 4226
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 683 1105 1154 1163 1242 1656 1904 1939 683
Size: 422 49 9 79 414 248 35 2970
Fragments arranged by size:
2970 422 414 248 79 49 35 9
BanII G_rGCy'C
Cuts at: 445 491 1146 1240 2270 445
Size: 46 655 94 1030 2401
Fragments arranged by size:
2401 1030 655 94 46
BbeI G_GCGC'C
Cuts at: 1109 1158 1167 1660 1908 1109
Size: 49 9 493 248 3427
Fragments arranged by size:
3427 493 248 49 9
BbsI GAAGACnn'nnnn_
Cuts at: 262 722 262
Size: 460 3766
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 1746 1746
Size: 4226
BglI GCCn_nnn'nGGC
Cuts at: 975 975
Size: 4226
BglII A'GATC_T
Cuts at: 301 1741 3778 301
Size: 1440 2037 749
Fragments arranged by size:
2037 1440 749
BlpI GC'TnA_GC
Cuts at: 1338 1338
Size: 4226
Bme1580I G_kGCm'C
Cuts at: 869 1088 1146 1851 1944 3000 869
Size: 219 58 705 93 1056 2095
Fragments arranged by size:
2095 1056 705 219 93 58
BmgBI CAC'GTC
Cuts at: 672 672
Size: 4226
BmrI ACTGGGnnnn_n'
Cuts at: 121 807 1438 1519 1854 3998 121
Size: 686 631 81 335 2144 349
Fragments arranged by size:
2144 686 631 349 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 601 772 1168 505
Size: 96 171 396 3563
Fragments arranged by size:
3563 396 171 96
Bpu10I CC'TnA_GC
Cuts at: 991 991
Size: 4226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 247 1402 2281 2773 3071 235
Size: 12 1155 879 492 298 1390
Fragments arranged by size:
1390 1155 879 492 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 262 3930 262
Size: 3668 558
BsaAI yAC'GTr
Cuts at: 1579 1579
Size: 4226
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 1106 1155 1164 1657 1905 2607 4103 1106
Size: 49 9 493 248 702 1496 1229
Fragments arranged by size:
1496 1229 702 493 248 49 9
BsaWI w'CCGG_w
Cuts at: 1415 1936 2614 2888 3035 3446 1415
Size: 521 678 274 147 411 2195
Fragments arranged by size:
2195 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 792 822 1056 1086 792
Size: 30 234 30 3932
Fragments arranged by size:
3932 234 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 769 1028 547
Size: 222 259 3745
Fragments arranged by size:
3745 259 222
BseYI C'CCAG_C
Cuts at: 978 1651 2409 2986 3482 978
Size: 673 758 577 496 1722
Fragments arranged by size:
1722 758 673 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 1110 175
Size: 935 3291
BsiEI CG_ry'CG
Cuts at: 104 936 970 1768 1814 3022 104
Size: 832 34 798 46 1208 1308
Fragments arranged by size:
1308 1208 832 798 46 34
BsiHKAI G_wGCw'C
Cuts at: 1240 2018 2208 3000 1240
Size: 778 190 792 2466
Fragments arranged by size:
2466 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1010 1010
Size: 4226
Bsp1286I G_dGCh'C
Cuts at: 445 491 869 1088 1146 1240 1851 1944 2018
Size: 46 378 219 58 94 611 93 74
Cuts at: 2018 2208 2270 3000 445
Size: 190 62 730 1671
Fragments arranged by size:
1671 730 611 378 219 190 94 93
74 62 58 46
BspEI T'CCGG_A
Cuts at: 3446 3446
Size: 4226
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 914 1209 1276 1490 1792 2173 914
Size: 295 67 214 302 381 2967
Fragments arranged by size:
2967 381 302 295 214 67
BsrBI CCG'CTC
Cuts at: 50 995 1152 2518 2572 4056 4221 50
Size: 945 157 1366 54 1484 165 55
Fragments arranged by size:
1484 1366 945 165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 2138 2138
Size: 4226
BsrFI r'CCGG_y
Cuts at: 719 2224 2405 2614 719
Size: 1505 181 209 2331
Fragments arranged by size:
2331 1505 209 181
BssHII G'CGCG_C
Cuts at: 376 877 376
Size: 501 3725
BssSI C'ACGA_G
Cuts at: 360 2497 2855 360
Size: 2137 358 1731
Fragments arranged by size:
2137 1731 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1603 1603
Size: 4226
BstBI TT'CG_AA
Cuts at: 2587 2587
Size: 4226
BstYI r'GATC_y
Cuts at: 1 301 1251 1718 1741 2076 2322 3334 3420
Size: 300 950 467 23 335 246 1012 86
Cuts at: 3420 3432 3778 1
Size: 12 346 449
Fragments arranged by size:
1012 950 467 449 346 335 300 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 690 690
Size: 4226
BtgI C'CryG_G
Cuts at: 107 366 869 906 953 1008 1159 107
Size: 259 503 37 47 55 151 3174
Fragments arranged by size:
3174 503 259 151 55 47 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2176 2357 2520 2176
Size: 181 163 3882
Fragments arranged by size:
3882 181 163
BtsI GCAGTG_nn'
Cuts at: 1469 1469
Size: 4226
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 967 1811 1985 2376 2403 3748 967
Size: 844 174 391 27 1345 1445
Fragments arranged by size:
1445 1345 844 391 174 27
EagI C'GGCC_G
Cuts at: 967 1811 967
Size: 844 3382
EarI CTCTTCn'nnn_
Cuts at: 2249 2459 2249
Size: 210 4016
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2434 2744 2890 4036 2434
Size: 310 146 1146 2624
Fragments arranged by size:
2624 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1238 1238
Size: 4226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 601 772 1168 2051 2483 3230 3498 505
Size: 96 171 396 883 432 747 268 1233
Fragments arranged by size:
1233 883 747 432 396 268 171 96
EcoNI CCTnn'n_nnAGG
Cuts at: 754 754
Size: 4226
EcoO109I rG'GnC_Cy
Cuts at: 115 1142 1143 1365 115
Size: 1027 1 222 2976
Fragments arranged by size:
2976 1027 222 1
EcoRI G'AATT_C
Cuts at: 238 1230 238
Size: 992 3234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 288 2245 288
Size: 1957 2269
FspI TGC'GCA
Cuts at: 223 2007 223
Size: 1784 2442
HaeII r_GCGC'y
Cuts at: 745 986 1109 1158 1167 1660 1908 2930 3909
Size: 241 123 49 9 493 248 1022 979
Cuts at: 3909 745
Size: 1062
Fragments arranged by size:
1062 1022 979 493 248 241 123 49
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 792 824 844 876 2068 2098 2100 2130 792
Size: 32 20 32 1192 30 2 30 2888
Fragments arranged by size:
2888 1192 32 32 30 30 20 2
HincII GTy'rAC
Cuts at: 1265 1265
Size: 4226
HindIII A'AGCT_T
Cuts at: 322 322
Size: 4226
KasI G'GCGC_C
Cuts at: 1105 1154 1163 1656 1904 1105
Size: 49 9 493 248 3427
Fragments arranged by size:
3427 493 248 49 9
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MluI A'CGCG_T
Cuts at: 348 3659 348
Size: 3311 915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 371 872 2897 3081 371
Size: 501 2025 184 1516
Fragments arranged by size:
2025 1516 501 184
MscI TGG'CCA
Cuts at: 1987 1987
Size: 4226
MslI CAynn'nnrTG
Cuts at: 217 2215 2342 217
Size: 1998 127 2101
Fragments arranged by size:
2101 1998 127
MspA1I CmG'CkG
Cuts at: 368 387 806 871 908 955 1121 1337 1651
Size: 19 419 65 37 47 166 216 314
Cuts at: 1651 2011 3024 3269 368
Size: 360 1013 245 1325
Fragments arranged by size:
1325 1013 419 360 314 245 216 166
65 47 37 19
NaeI GCC'GGC
Cuts at: 2407 2407
Size: 4226
NarI GG'CG_CC
Cuts at: 1106 1155 1164 1657 1905 1106
Size: 49 9 493 248 3427
Fragments arranged by size:
3427 493 248 49 9
NcoI C'CATG_G
Cuts at: 107 107
Size: 4226
NgoMIV G'CCGG_C
Cuts at: 2405 2405
Size: 4226
NspI r_CATG'y
Cuts at: 1279 1779 2686 1279
Size: 500 907 2819
Fragments arranged by size:
2819 907 500
PciI A'CATG_T
Cuts at: 2682 2682
Size: 4226
PflMI CCAn_nnn'nTGG
Cuts at: 579 579
Size: 4226
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PspOMI G'GGCC_C
Cuts at: 1142 1142
Size: 4226
PspXI vC'TCGA_Gb
Cuts at: 1178 1178
Size: 4226
PstI C_TGCA'G
Cuts at: 386 426 608 1273 1958 386
Size: 40 182 665 685 2654
Fragments arranged by size:
2654 685 665 182 40
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 387 1651 2011 387
Size: 1264 360 2602
Fragments arranged by size:
2602 1264 360
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 1240 1240
Size: 4226
SacII CC_GC'GG
Cuts at: 369 872 909 956 369
Size: 503 37 47 3639
Fragments arranged by size:
3639 503 47 37
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 2249 2459 2249
Size: 210 4016
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 3743 3743
Size: 4226
SfcI C'TryA_G
Cuts at: 33 382 422 604 1269 1954 2947 3138 33
Size: 349 40 182 665 685 993 191 1121
Fragments arranged by size:
1121 993 685 665 349 191 182 40
SfoI GGC'GCC
Cuts at: 1107 1156 1165 1658 1906 1107
Size: 49 9 493 248 3427
Fragments arranged by size:
3427 493 248 49 9
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 767 1039 1248 767
Size: 272 209 3745
Fragments arranged by size:
3745 272 209
SmlI C'TyrA_G
Cuts at: 250 262 1178 1381 2296 2788 3050 250
Size: 12 916 203 915 492 262 1426
Fragments arranged by size:
1426 916 915 492 262 203 12
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1279 1779 1279
Size: 500 3726
StuI AGG'CCT
Cuts at: 639 639
Size: 4226
StyI C'CwwG_G
Cuts at: 107 771 795 1360 1714 107
Size: 664 24 565 354 2619
Fragments arranged by size:
2619 664 565 354 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1809 1942 2401 2610 1809
Size: 133 459 209 3425
Fragments arranged by size:
3425 459 209 133
TatI w'GTAC_w
Cuts at: 3741 3741
Size: 4226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1056 1088 3743 3775 1056
Size: 32 2655 32 1507
Fragments arranged by size:
2655 1507 32 32
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 576 1721 576
Size: 1145 3081
XhoI C'TCGA_G
Cuts at: 1178 1178
Size: 4226
XmaI C'CCGG_G
Cuts at: 765 1037 1246 765
Size: 272 209 3745
Fragments arranged by size:
3745 272 209
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI BaeI
BbvCI BcgI BfrBI BmtI BsiWI BsmI BsrGI BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI