Restriction map for
pF1KE0626
(Circular) (Six-base) MAPSORT of: pF1KE0626.seq Check: 5612 from: 1 to: 4229
pF1KE0626 4229 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 910 1267 910
Size: 357 3872
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2486 3233 3501 2054
Size: 432 747 268 2782
Fragments arranged by size:
2782 747 432 268
AflIII A'CryG_T
Cuts at: 252 1581 2685 3662 252
Size: 1329 1104 977 819
Fragments arranged by size:
1329 1104 977 819
AgeI A'CCGG_T
Cuts at: 2617 2617
Size: 4229
AlwNI CAG_nnn'CTG
Cuts at: 143 1534 3101 143
Size: 1391 1567 1271
Fragments arranged by size:
1567 1391 1271
ApaLI G'TGCA_C
Cuts at: 2999 2999
Size: 4229
ApoI r'AATT_y
Cuts at: 184 223 1162 1233 3582 3838 184
Size: 39 939 71 2349 256 575
Fragments arranged by size:
2349 939 575 256 71 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 1249 1249
Size: 4229
AvrII C'CTAG_G
Cuts at: 612 612
Size: 4229
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4229
BanI G'GyrC_C
Cuts at: 1245 1659 1907 1942 1245
Size: 414 248 35 3532
Fragments arranged by size:
3532 414 248 35
BanII G_rGCy'C
Cuts at: 1243 2273 1243
Size: 1030 3199
BbeI G_GCGC'C
Cuts at: 1663 1911 1663
Size: 248 3981
BbsI GAAGACnn'nnnn_
Cuts at: 527 980 1010 527
Size: 453 30 3746
Fragments arranged by size:
3746 453 30
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2888 4229 2127
Size: 761 1341 2127
Fragments arranged by size:
2127 1341 761
BclI T'GATC_A
Cuts at: 792 1749 792
Size: 957 3272
BglI GCCn_nnn'nGGC
Cuts at: 929 929
Size: 4229
BglII A'GATC_T
Cuts at: 1744 3781 1744
Size: 2037 2192
BlpI GC'TnA_GC
Cuts at: 1081 1131 1341 1081
Size: 50 210 3969
Fragments arranged by size:
3969 210 50
Bme1580I G_kGCm'C
Cuts at: 154 1854 1947 3003 154
Size: 1700 93 1056 1380
Fragments arranged by size:
1700 1380 1056 93
BmgBI CAC'GTC
Cuts at: 549 549
Size: 4229
BmrI ACTGGGnnnn_n'
Cuts at: 1441 1522 1857 4001 1441
Size: 81 335 2144 1669
Fragments arranged by size:
2144 1669 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 1078 1163 621
Size: 457 85 3687
Fragments arranged by size:
3687 457 85
Bpu10I CC'TnA_GC
Cuts at: 373 373
Size: 4229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2284 2776 3074 1405
Size: 879 492 298 2560
Fragments arranged by size:
2560 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 840 3933 840
Size: 3093 1136
BsaAI yAC'GTr
Cuts at: 1582 1582
Size: 4229
BsaBI GATnn'nnATC
Cuts at: 6 338 1743 3780 6
Size: 332 1405 2037 455
Fragments arranged by size:
2037 1405 455 332
BsaHI Gr'CG_yC
Cuts at: 1660 1908 2610 4106 1660
Size: 248 702 1496 1783
Fragments arranged by size:
1783 1496 702 248
BsaWI w'CCGG_w
Cuts at: 901 1418 1939 2617 2891 3038 3449 901
Size: 517 521 678 274 147 411 1681
Fragments arranged by size:
1681 678 521 517 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1161 1191 1161
Size: 30 4199
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 244
Size: 4229
BseYI C'CCAG_C
Cuts at: 1654 2412 2989 3485 1654
Size: 758 577 496 2398
Fragments arranged by size:
2398 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3025 104
Size: 1667 46 1208 1308
Fragments arranged by size:
1667 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1243 2021 2211 3003 1243
Size: 778 190 792 2469
Fragments arranged by size:
2469 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 154 1243 1854 1947 2021 2211 2273 3003 154
Size: 1089 611 93 74 190 62 730 1380
Fragments arranged by size:
1380 1089 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3449 3449
Size: 4229
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1279 1493 1795 2176 1279
Size: 214 302 381 3332
Fragments arranged by size:
3332 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2521 2575 4059 4224 50
Size: 2471 54 1484 165 55
Fragments arranged by size:
2471 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 2141
Size: 4229
BsrFI r'CCGG_y
Cuts at: 122 849 2227 2408 2617 122
Size: 727 1378 181 209 1734
Fragments arranged by size:
1734 1378 727 209 181
BssSI C'ACGA_G
Cuts at: 2500 2858 2500
Size: 358 3871
BstAPI GCAn_nnn'nTGC
Cuts at: 143 1606 143
Size: 1463 2766
BstBI TT'CG_AA
Cuts at: 2590 2590
Size: 4229
BstYI r'GATC_y
Cuts at: 1 263 680 1254 1721 1744 2079 2325 3337
Size: 262 417 574 467 23 335 246 1012
Cuts at: 3337 3423 3435 3781 1
Size: 86 12 346 449
Fragments arranged by size:
1012 574 467 449 417 346 335 262
246 86 23 12
BtgI C'CryG_G
Cuts at: 303 303
Size: 4229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2179 2360 2523 2179
Size: 181 163 3885
Fragments arranged by size:
3885 181 163
BtsI GCAGTG_nn'
Cuts at: 367 1472 367
Size: 1105 3124
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 1935 2793 1935
Size: 858 3371
EaeI y'GGCC_r
Cuts at: 359 1814 1988 2379 2406 3751 359
Size: 1455 174 391 27 1345 837
Fragments arranged by size:
1455 1345 837 391 174 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4229
EarI CTCTTCn'nnn_
Cuts at: 160 778 2252 2462 160
Size: 618 1474 210 1927
Fragments arranged by size:
1927 1474 618 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2437 2747 2893 4039 2437
Size: 310 146 1146 2627
Fragments arranged by size:
2627 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1241 1241
Size: 4229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 1078 1163 2054 2486 3233 3501 621
Size: 457 85 891 432 747 268 1349
Fragments arranged by size:
1349 891 747 457 432 268 85
EcoO109I rG'GnC_Cy
Cuts at: 1368 1368
Size: 4229
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 4229
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4229
HaeII r_GCGC'y
Cuts at: 1663 1911 2933 3912 1663
Size: 248 1022 979 1980
Fragments arranged by size:
1980 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 608 640 777 809 867 899 2071 2101 2103
Size: 32 137 32 58 32 1172 30 2
Cuts at: 2103 2133 608
Size: 30 2704
Fragments arranged by size:
2704 1172 137 58 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 276 1268 276
Size: 992 3237
HindIII A'AGCT_T
Cuts at: 954 1083 954
Size: 129 4100
KasI G'GCGC_C
Cuts at: 1659 1907 1659
Size: 248 3981
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 865 2900 3084 865
Size: 2035 184 2010
Fragments arranged by size:
2035 2010 184
MscI TGG'CCA
Cuts at: 1990 1990
Size: 4229
MslI CAynn'nnrTG
Cuts at: 508 841 2218 2345 508
Size: 333 1377 127 2392
Fragments arranged by size:
2392 1377 333 127
MspA1I CmG'CkG
Cuts at: 140 1340 1654 2014 3027 3272 140
Size: 1200 314 360 1013 245 1097
Fragments arranged by size:
1200 1097 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2410 2410
Size: 4229
NarI GG'CG_CC
Cuts at: 1660 1908 1660
Size: 248 3981
NcoI C'CATG_G
Cuts at: 303 303
Size: 4229
NgoMIV G'CCGG_C
Cuts at: 2408 2408
Size: 4229
NspI r_CATG'y
Cuts at: 256 1177 1282 1782 2689 256
Size: 921 105 500 907 1796
Fragments arranged by size:
1796 921 907 500 105
PciI A'CATG_T
Cuts at: 252 2685 252
Size: 2433 1796
PfoI T'CCnGG_A
Cuts at: 626 626
Size: 4229
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 4197
PshAI GACnn'nnGTC
Cuts at: 531 531
Size: 4229
PstI C_TGCA'G
Cuts at: 1276 1961 1276
Size: 685 3544
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 1654 2014 1654
Size: 360 3869
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 1243 1243
Size: 4229
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 160 778 2252 2462 160
Size: 618 1474 210 1927
Fragments arranged by size:
1927 1474 618 210
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 3746 3746
Size: 4229
SfcI C'TryA_G
Cuts at: 33 408 1272 1957 2950 3141 33
Size: 375 864 685 993 191 1121
Fragments arranged by size:
1121 993 864 685 375 191
SfoI GGC'GCC
Cuts at: 1661 1909 1661
Size: 248 3981
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4229
SmlI C'TyrA_G
Cuts at: 1384 2299 2791 3053 1384
Size: 915 492 262 2560
Fragments arranged by size:
2560 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3729
StyI C'CwwG_G
Cuts at: 303 612 1363 1717 303
Size: 309 751 354 2815
Fragments arranged by size:
2815 751 354 309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1812 1945 2404 2613 1812
Size: 133 459 209 3428
Fragments arranged by size:
3428 459 209 133
TatI w'GTAC_w
Cuts at: 721 3744 721
Size: 3023 1206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4197
Tth111I GACn'n_nGTC
Cuts at: 2026 2026
Size: 4229
XbaI T'CTAG_A
Cuts at: 1260 1260
Size: 4229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1724 1724
Size: 4229
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4229
XmnI GAAnn'nnTTC
Cuts at: 671 671
Size: 4229
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI