Restriction map for
pF1KE0620
(Circular) (Six-base) MAPSORT of: pF1KE0620.seq Check: 3603 from: 1 to: 4193
pF1KE0620 4193 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1231 1231
Size: 4193
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 4033 4033
Size: 4193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2018 2450 3197 3465 2018
Size: 432 747 268 2746
Fragments arranged by size:
2746 747 432 268
AflIII A'CryG_T
Cuts at: 1545 2649 3626 1545
Size: 1104 977 2112
Fragments arranged by size:
2112 1104 977
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AhdI GACnn_n'nnGTC
Cuts at: 303 303
Size: 4193
AlwNI CAG_nnn'CTG
Cuts at: 123 747 1498 3065 123
Size: 624 751 1567 1251
Fragments arranged by size:
1567 1251 751 624
ApaI G_GGCC'C
Cuts at: 157 157
Size: 4193
ApaLI G'TGCA_C
Cuts at: 2963 2963
Size: 4193
ApoI r'AATT_y
Cuts at: 1197 3546 3802 1197
Size: 2349 256 1588
Fragments arranged by size:
2349 1588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 602 1213 602
Size: 611 3582
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 928 1209 1623 1871 1906 928
Size: 281 414 248 35 3215
Fragments arranged by size:
3215 414 281 248 35
BanII G_rGCy'C
Cuts at: 157 503 698 1207 2237 157
Size: 346 195 509 1030 2113
Fragments arranged by size:
2113 1030 509 346 195
BbeI G_GCGC'C
Cuts at: 932 1627 1875 932
Size: 695 248 3250
Fragments arranged by size:
3250 695 248
BbsI GAAGACnn'nnnn_
Cuts at: 436 739 436
Size: 303 3890
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1047 1081 1047
Size: 34 4159
BciVI GTATCCnnnnn_n'
Cuts at: 2091 2852 4193 2091
Size: 761 1341 2091
Fragments arranged by size:
2091 1341 761
BclI T'GATC_A
Cuts at: 1713 1713
Size: 4193
BglI GCCn_nnn'nGGC
Cuts at: 161 166 180 649 899 1124 161
Size: 5 14 469 250 225 3230
Fragments arranged by size:
3230 469 250 225 14 5
BglII A'GATC_T
Cuts at: 1708 3745 1708
Size: 2037 2156
BlpI GC'TnA_GC
Cuts at: 692 1305 692
Size: 613 3580
Bme1580I G_kGCm'C
Cuts at: 157 1818 1911 2967 157
Size: 1661 93 1056 1383
Fragments arranged by size:
1661 1383 1056 93
BmgBI CAC'GTC
Cuts at: 566 566
Size: 4193
BmrI ACTGGGnnnn_n'
Cuts at: 133 452 540 1405 1486 1821 3965 133
Size: 319 88 865 81 335 2144 361
Fragments arranged by size:
2144 865 361 335 319 88 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1369 2248 2740 3038 1369
Size: 879 492 298 2524
Fragments arranged by size:
2524 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1161 3897 1161
Size: 2736 1457
BsaAI yAC'GTr
Cuts at: 1546 1546
Size: 4193
BsaBI GATnn'nnATC
Cuts at: 6 874 1707 3744 6
Size: 868 833 2037 455
Fragments arranged by size:
2037 868 833 455
BsaHI Gr'CG_yC
Cuts at: 851 929 1624 1872 2574 4070 851
Size: 78 695 248 702 1496 974
Fragments arranged by size:
1496 974 702 695 248 78
BsaWI w'CCGG_w
Cuts at: 963 1382 1903 2581 2855 3002 3413 963
Size: 419 521 678 274 147 411 1743
Fragments arranged by size:
1743 678 521 419 411 274 147
BseYI C'CCAG_C
Cuts at: 681 809 1618 2376 2953 3449 681
Size: 128 809 758 577 496 1425
Fragments arranged by size:
1425 809 758 577 496 128
BsiEI CG_ry'CG
Cuts at: 104 161 555 872 1735 1781 2989 104
Size: 57 394 317 863 46 1208 1308
Fragments arranged by size:
1308 1208 863 394 317 57 46
BsiHKAI G_wGCw'C
Cuts at: 1207 1985 2175 2967 1207
Size: 778 190 792 2433
Fragments arranged by size:
2433 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 157 503 698 1207 1818 1911 1985 2175 2237
Size: 346 195 509 611 93 74 190 62
Cuts at: 2237 2967 157
Size: 730 1383
Fragments arranged by size:
1383 730 611 509 346 195 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3413 3413
Size: 4193
BspHI T'CATG_A
Cuts at: 3369 3369
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 251 1143 1243 1457 1759 2140 251
Size: 892 100 214 302 381 2304
Fragments arranged by size:
2304 892 381 302 214 100
BsrBI CCG'CTC
Cuts at: 50 890 2485 2539 4023 4188 50
Size: 840 1595 54 1484 165 55
Fragments arranged by size:
1595 1484 840 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2105 2105
Size: 4193
BsrFI r'CCGG_y
Cuts at: 160 931 2191 2372 2581 160
Size: 771 1260 181 209 1772
Fragments arranged by size:
1772 1260 771 209 181
BsrGI T'GTAC_A
Cuts at: 950 950
Size: 4193
BssSI C'ACGA_G
Cuts at: 2464 2822 2464
Size: 358 3835
BstAPI GCAn_nnn'nTGC
Cuts at: 747 1570 747
Size: 823 3370
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstYI r'GATC_y
Cuts at: 1 1218 1685 1708 2043 2289 3301 3387 3399
Size: 1217 467 23 335 246 1012 86 12
Cuts at: 3399 3745 1
Size: 346 449
Fragments arranged by size:
1217 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 131 1103 107
Size: 24 972 3197
Fragments arranged by size:
3197 972 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1095 2143 2324 2487 1095
Size: 1048 181 163 2801
Fragments arranged by size:
2801 1048 181 163
BtsI GCAGTG_nn'
Cuts at: 1436 1436
Size: 4193
ClaI AT'CG_AT
Cuts at: 878 878
Size: 4193
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 1899 2757 1899
Size: 858 3335
EaeI y'GGCC_r
Cuts at: 158 552 1100 1106 1778 1952 2343 2370 3715
Size: 394 548 6 672 174 391 27 1345
Cuts at: 3715 158
Size: 636
Fragments arranged by size:
1345 672 636 548 394 391 174 27
6
EagI C'GGCC_G
Cuts at: 158 552 1778 158
Size: 394 1226 2573
Fragments arranged by size:
2573 1226 394
EarI CTCTTCn'nnn_
Cuts at: 286 544 641 2216 2426 286
Size: 258 97 1575 210 2053
Fragments arranged by size:
2053 1575 258 210 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 762 2401 2711 2857 4003 762
Size: 1639 310 146 1146 952
Fragments arranged by size:
1639 1146 952 310 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2018 2450 3197 3465 2018
Size: 432 747 268 2746
Fragments arranged by size:
2746 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 153 790 1332 153
Size: 637 542 3014
Fragments arranged by size:
3014 637 542
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 297 2212 297
Size: 1915 2278
FseI GG_CCGG'CC
Cuts at: 164 164
Size: 4193
FspI TGC'GCA
Cuts at: 1974 1974
Size: 4193
HaeII r_GCGC'y
Cuts at: 748 783 932 1627 1875 2897 3876 748
Size: 35 149 695 248 1022 979 1065
Fragments arranged by size:
1065 1022 979 695 248 149 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 2035 2065 2067 2097 289
Size: 32 1714 30 2 30 2385
Fragments arranged by size:
2385 1714 32 30 30 2
HincII GTy'rAC
Cuts at: 237 1232 237
Size: 995 3198
KasI G'GCGC_C
Cuts at: 928 1623 1871 928
Size: 695 248 3250
Fragments arranged by size:
3250 695 248
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2864 3048 2864
Size: 184 4009
MscI TGG'CCA
Cuts at: 1108 1954 1108
Size: 846 3347
MslI CAynn'nnrTG
Cuts at: 439 577 946 2182 2309 439
Size: 138 369 1236 127 2323
Fragments arranged by size:
2323 1236 369 138 127
MspA1I CmG'CkG
Cuts at: 497 681 691 993 1304 1618 1978 2991 3236
Size: 184 10 302 311 314 360 1013 245
Cuts at: 3236 497
Size: 1454
Fragments arranged by size:
1454 1013 360 314 311 302 245 184
10
NaeI GCC'GGC
Cuts at: 162 933 2374 162
Size: 771 1441 1981
Fragments arranged by size:
1981 1441 771
NarI GG'CG_CC
Cuts at: 929 1624 1872 929
Size: 695 248 3250
Fragments arranged by size:
3250 695 248
NcoI C'CATG_G
Cuts at: 107 131 1103 107
Size: 24 972 3197
Fragments arranged by size:
3197 972 24
NgoMIV G'CCGG_C
Cuts at: 160 931 2372 160
Size: 771 1441 1981
Fragments arranged by size:
1981 1441 771
NruI TCG'CGA
Cuts at: 715 715
Size: 4193
NspI r_CATG'y
Cuts at: 1246 1746 2653 1246
Size: 500 907 2786
Fragments arranged by size:
2786 907 500
PciI A'CATG_T
Cuts at: 2649 2649
Size: 4193
PflMI CCAn_nnn'nTGG
Cuts at: 190 190
Size: 4193
PfoI T'CCnGG_A
Cuts at: 473 473
Size: 4193
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 4161
PspOMI G'GGCC_C
Cuts at: 153 153
Size: 4193
PstI C_TGCA'G
Cuts at: 1154 1240 1925 1154
Size: 86 685 3422
Fragments arranged by size:
3422 685 86
PvuI CG_AT'CG
Cuts at: 104 872 104
Size: 768 3425
PvuII CAG'CTG
Cuts at: 691 993 1618 1978 691
Size: 302 625 360 2906
Fragments arranged by size:
2906 625 360 302
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 544 2216 2426 544
Size: 1672 210 2311
Fragments arranged by size:
2311 1672 210
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SexAI A'CCwGG_T
Cuts at: 584 584
Size: 4193
SfcI C'TryA_G
Cuts at: 33 1150 1236 1921 2914 3105 33
Size: 1117 86 685 993 191 1121
Fragments arranged by size:
1121 1117 993 685 191 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 161 166 161
Size: 5 4188
SfoI GGC'GCC
Cuts at: 930 1625 1873 930
Size: 695 248 3250
Fragments arranged by size:
3250 695 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 1348 2263 2755 3017 1348
Size: 915 492 262 2524
Fragments arranged by size:
2524 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StyI C'CwwG_G
Cuts at: 107 131 189 345 1103 1327 1681 107
Size: 24 58 156 758 224 354 2619
Fragments arranged by size:
2619 758 354 224 156 58 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 769 1776 1909 2368 2577 769
Size: 1007 133 459 209 2385
Fragments arranged by size:
2385 1007 459 209 133
TatI w'GTAC_w
Cuts at: 950 3708 950
Size: 2758 1435
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4161
Tth111I GACn'n_nGTC
Cuts at: 586 1990 586
Size: 1404 2789
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1688 1688
Size: 4193
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmtI BpmI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI