Restriction map for
pF1KE0616
(Circular) (Six-base) MAPSORT of: pF1KE0616.seq Check: 3680 from: 1 to: 4061
pF1KE0616 4061 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4061
Acc65I G'GTAC_C
Cuts at: 454 1077 454
Size: 623 3438
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 407 705 1886 2318 3065 3333 135
Size: 272 298 1181 432 747 268 863
Fragments arranged by size:
1181 863 747 432 298 272 268
AfeI AGC'GCT
Cuts at: 528 528
Size: 4061
AflIII A'CryG_T
Cuts at: 659 1413 2517 3494 659
Size: 754 1104 977 1226
Fragments arranged by size:
1226 1104 977 754
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 1366 2933 1366
Size: 1567 2494
ApaI G_GGCC'C
Cuts at: 136 477 136
Size: 341 3720
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 439 1065 3414 3670 439
Size: 626 2349 256 830
Fragments arranged by size:
2349 830 626 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 129 649 1081 129
Size: 520 432 3109
Fragments arranged by size:
3109 520 432
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 454 642 902 1077 1491 1739 1774 454
Size: 188 260 175 414 248 35 2741
Fragments arranged by size:
2741 414 260 248 188 175 35
BanII G_rGCy'C
Cuts at: 136 477 554 632 695 718 792 1005 1075
Size: 341 77 78 63 23 74 213 70
Cuts at: 1075 2105 136
Size: 1030 2092
Fragments arranged by size:
2092 1030 341 213 78 77 74 70
63 23
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4027
BciVI GTATCCnnnnn_n'
Cuts at: 1055 1959 2720 4061 1055
Size: 904 761 1341 1055
Fragments arranged by size:
1341 1055 904 761
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglI GCCn_nnn'nGGC
Cuts at: 549 713 549
Size: 164 3897
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 136 477 647 828 1686 1779 2835 136
Size: 341 170 181 858 93 1056 1362
Fragments arranged by size:
1362 1056 858 341 181 170 93
BmgBI CAC'GTC
Cuts at: 660 660
Size: 4061
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 715 263
Size: 452 3609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1237 2116 2608 2906 928
Size: 309 879 492 298 2083
Fragments arranged by size:
2083 879 492 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 377 607 3765 377
Size: 230 3158 673
Fragments arranged by size:
3158 673 230
BsaAI yAC'GTr
Cuts at: 1414 1414
Size: 4061
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 665 792 1250 1771 2449 2723 2870 3281 665
Size: 127 458 521 678 274 147 411 1445
Fragments arranged by size:
1445 678 521 458 411 274 147 127
BseYI C'CCAG_C
Cuts at: 476 1486 2244 2821 3317 476
Size: 1010 758 577 496 1220
Fragments arranged by size:
1220 1010 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 731 792 1075 1853 2043 2835 731
Size: 61 283 778 190 792 1957
Fragments arranged by size:
1957 792 778 283 190 61
Bsp1286I G_dGCh'C
Cuts at: 136 477 554 632 647 695 718 731 792
Size: 341 77 78 15 48 23 13 61
Cuts at: 792 828 1005 1075 1686 1779 1853 2043 2105
Size: 36 177 70 611 93 74 190 62
Cuts at: 2105 2835 136
Size: 730 1362
Fragments arranged by size:
1362 730 611 341 190 177 93 78
77 74 70 62 61 48 36 23
15 13
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1111 1325 1627 2008 1111
Size: 214 302 381 3164
Fragments arranged by size:
3164 381 302 214
BsrBI CCG'CTC
Cuts at: 50 840 2353 2407 3891 4056 50
Size: 790 1513 54 1484 165 55
Fragments arranged by size:
1513 1484 790 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1973 1973
Size: 4061
BsrFI r'CCGG_y
Cuts at: 899 2059 2240 2449 899
Size: 1160 181 209 2511
Fragments arranged by size:
2511 1160 209 181
BssSI C'ACGA_G
Cuts at: 2332 2690 2332
Size: 358 3703
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstYI r'GATC_y
Cuts at: 1 1086 1553 1576 1911 2157 3169 3255 3267
Size: 1085 467 23 335 246 1012 86 12
Cuts at: 3267 3613 1
Size: 346 449
Fragments arranged by size:
1085 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 807 807
Size: 4061
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 1304 1304
Size: 4061
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 1646 1820 2211 2238 3583 1646
Size: 174 391 27 1345 2124
Fragments arranged by size:
2124 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 790 1073 790
Size: 283 3778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 263 407 705 715 1886 2318 3065 3333
Size: 128 144 298 10 1171 432 747 268
Cuts at: 3333 135
Size: 863
Fragments arranged by size:
1171 863 747 432 298 268 144 128
10
EcoO109I rG'GnC_Cy
Cuts at: 133 473 1200 133
Size: 340 727 2994
Fragments arranged by size:
2994 727 340
EcoRI G'AATT_C
Cuts at: 439 1065 439
Size: 626 3435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 598 975 2080 598
Size: 377 1105 2579
Fragments arranged by size:
2579 1105 377
FspI TGC'GCA
Cuts at: 302 1842 302
Size: 1540 2521
HaeII r_GCGC'y
Cuts at: 530 1495 1743 2765 3744 530
Size: 965 248 1022 979 847
Fragments arranged by size:
1022 979 965 847 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 236 268 736 768 1903 1933 1935 1965 236
Size: 32 468 32 1135 30 2 30 2332
Fragments arranged by size:
2332 1135 468 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 458 1081 458
Size: 623 3438
MfeI C'AATT_G
Cuts at: 545 545
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 635 2732 2916 635
Size: 2097 184 1780
Fragments arranged by size:
2097 1780 184
MscI TGG'CCA
Cuts at: 1822 1822
Size: 4061
MslI CAynn'nnrTG
Cuts at: 296 2050 2177 296
Size: 1754 127 2180
Fragments arranged by size:
2180 1754 127
MspA1I CmG'CkG
Cuts at: 226 333 493 594 1172 1486 1846 2859 3104
Size: 107 160 101 578 314 360 1013 245
Cuts at: 3104 226
Size: 1183
Fragments arranged by size:
1183 1013 578 360 314 245 160 107
101
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 807 807
Size: 4061
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NspI r_CATG'y
Cuts at: 1114 1614 2521 1114
Size: 500 907 2654
Fragments arranged by size:
2654 907 500
PasI CC'CwG_GG
Cuts at: 143 143
Size: 4061
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PflMI CCAn_nnn'nTGG
Cuts at: 186 738 186
Size: 552 3509
PfoI T'CCnGG_A
Cuts at: 240 240
Size: 4061
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 4029
PshAI GACnn'nnGTC
Cuts at: 802 802
Size: 4061
PspOMI G'GGCC_C
Cuts at: 132 473 132
Size: 341 3720
PstI C_TGCA'G
Cuts at: 1108 1793 1108
Size: 685 3376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 226 333 493 594 1486 1846 226
Size: 107 160 101 892 360 2441
Fragments arranged by size:
2441 892 360 160 107 101
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 792 1075 792
Size: 283 3778
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 1104 1789 2782 2973 33
Size: 1071 685 993 191 1121
Fragments arranged by size:
1121 1071 993 685 191
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 131 1083 131
Size: 952 3109
SmlI C'TyrA_G
Cuts at: 943 1216 2131 2623 2885 943
Size: 273 915 492 262 2119
Fragments arranged by size:
2119 915 492 273 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 807 1195 1549 807
Size: 388 354 3319
Fragments arranged by size:
3319 388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1644 1777 2236 2445 1644
Size: 133 459 209 3260
Fragments arranged by size:
3260 459 209 133
TatI w'GTAC_w
Cuts at: 980 3576 980
Size: 2596 1465
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1029 1061 3578 3610 1029
Size: 32 2517 32 1480
Fragments arranged by size:
2517 1480 32 32
Tth111I GACn'n_nGTC
Cuts at: 279 1033 1858 279
Size: 754 825 2482
Fragments arranged by size:
2482 825 754
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1556 1556
Size: 4061
XmaI C'CCGG_G
Cuts at: 129 1081 129
Size: 952 3109
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI