Restriction map for
pF1KE0614
(Circular) (Six-base) MAPSORT of: pF1KE0614.seq Check: 6972 from: 1 to: 4205
pF1KE0614 4205 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1243 1243
Size: 4205
Acc65I G'GTAC_C
Cuts at: 1221 1221
Size: 4205
AclI AA'CG_TT
Cuts at: 4045 4045
Size: 4205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 2030 2462 3209 3477 898
Size: 1132 432 747 268 1626
Fragments arranged by size:
1626 1132 747 432 268
AflIII A'CryG_T
Cuts at: 950 1557 2661 3638 950
Size: 607 1104 977 1517
Fragments arranged by size:
1517 1104 977 607
AgeI A'CCGG_T
Cuts at: 2593 2593
Size: 4205
AlwNI CAG_nnn'CTG
Cuts at: 1510 3077 1510
Size: 1567 2638
ApaLI G'TGCA_C
Cuts at: 2975 2975
Size: 4205
ApoI r'AATT_y
Cuts at: 988 1009 1209 3558 3814 988
Size: 21 200 2349 256 1379
Fragments arranged by size:
2349 1379 256 200 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 1225 1225
Size: 4205
BamHI G'GATC_C
Cuts at: 1230 1230
Size: 4205
BanI G'GyrC_C
Cuts at: 702 1221 1635 1883 1918 702
Size: 519 414 248 35 2989
Fragments arranged by size:
2989 519 414 248 35
BanII G_rGCy'C
Cuts at: 1219 2249 1219
Size: 1030 3175
BbeI G_GCGC'C
Cuts at: 1639 1887 1639
Size: 248 3957
BbsI GAAGACnn'nnnn_
Cuts at: 723 865 886 723
Size: 142 21 4042
Fragments arranged by size:
4042 142 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 178 212 178
Size: 34 4171
BciVI GTATCCnnnnn_n'
Cuts at: 852 2103 2864 4205 852
Size: 1251 761 1341 852
Fragments arranged by size:
1341 1251 852 761
BclI T'GATC_A
Cuts at: 1725 1725
Size: 4205
BglII A'GATC_T
Cuts at: 1720 3757 1720
Size: 2037 2168
BlpI GC'TnA_GC
Cuts at: 1317 1317
Size: 4205
Bme1580I G_kGCm'C
Cuts at: 1830 1923 2979 1830
Size: 93 1056 3056
Fragments arranged by size:
3056 1056 93
BmgBI CAC'GTC
Cuts at: 951 972 951
Size: 21 4184
BmrI ACTGGGnnnn_n'
Cuts at: 144 647 1417 1498 1833 3977 144
Size: 503 770 81 335 2144 372
Fragments arranged by size:
2144 770 503 372 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 842 2572 842
Size: 1730 2475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1190
Size: 4205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1381 2260 2752 3050 1381
Size: 879 492 298 2536
Fragments arranged by size:
2536 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3909 3909
Size: 4205
BsaAI yAC'GTr
Cuts at: 1558 1558
Size: 4205
BsaBI GATnn'nnATC
Cuts at: 6 1719 3756 6
Size: 1713 2037 455
Fragments arranged by size:
2037 1713 455
BsaHI Gr'CG_yC
Cuts at: 1636 1884 2586 4082 1636
Size: 248 702 1496 1759
Fragments arranged by size:
1759 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1394 1915 2593 2867 3014 3425 1394
Size: 521 678 274 147 411 2174
Fragments arranged by size:
2174 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 400 1630 2388 2965 3461 400
Size: 1230 758 577 496 1144
Fragments arranged by size:
1230 1144 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1747 1793 3001 104
Size: 1643 46 1208 1308
Fragments arranged by size:
1643 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1219 1997 2187 2979 1219
Size: 778 190 792 2445
Fragments arranged by size:
2445 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1219 1830 1923 1997 2187 2249 2979 1219
Size: 611 93 74 190 62 730 2445
Fragments arranged by size:
2445 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3425 3425
Size: 4205
BspHI T'CATG_A
Cuts at: 3381 3381
Size: 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1255 1469 1771 2152 1255
Size: 214 302 381 3308
Fragments arranged by size:
3308 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1136 2497 2551 4035 4200 50
Size: 1086 1361 54 1484 165 55
Fragments arranged by size:
1484 1361 1086 165 55 54
BsrDI GCAATG_nn'
Cuts at: 194 1188 2117 194
Size: 994 929 2282
Fragments arranged by size:
2282 994 929
BsrFI r'CCGG_y
Cuts at: 590 1111 2203 2384 2593 590
Size: 521 1092 181 209 2202
Fragments arranged by size:
2202 1092 521 209 181
BssSI C'ACGA_G
Cuts at: 2476 2834 2476
Size: 358 3847
BstAPI GCAn_nnn'nTGC
Cuts at: 1582 1582
Size: 4205
BstBI TT'CG_AA
Cuts at: 2566 2566
Size: 4205
BstEII G'GTnAC_C
Cuts at: 753 753
Size: 4205
BstXI CCAn_nnnn'nTGG
Cuts at: 312 525 639 312
Size: 213 114 3878
Fragments arranged by size:
3878 213 114
BstYI r'GATC_y
Cuts at: 1 1079 1230 1697 1720 2055 2301 3313 3399
Size: 1078 151 467 23 335 246 1012 86
Cuts at: 3399 3411 3757 1
Size: 12 346 449
Fragments arranged by size:
1078 1012 467 449 346 335 246 151
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 1104 1448 1104
Size: 344 3861
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4205
DraIII CAC_nnn'GTG
Cuts at: 1194 1194
Size: 4205
DrdI GACnn_nn'nnGTC
Cuts at: 1911 2769 1911
Size: 858 3347
EaeI y'GGCC_r
Cuts at: 302 515 629 1790 1964 2355 2382 3727 302
Size: 213 114 1161 174 391 27 1345 780
Fragments arranged by size:
1345 1161 780 391 213 174 114 27
EagI C'GGCC_G
Cuts at: 1790 1790
Size: 4205
EarI CTCTTCn'nnn_
Cuts at: 2228 2438 2228
Size: 210 3995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 864 2413 2723 2869 4015 864
Size: 1549 310 146 1146 1054
Fragments arranged by size:
1549 1146 1054 310 146
EcoICRI GAG'CTC
Cuts at: 1217 1217
Size: 4205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1190 2030 2462 3209 3477 898
Size: 292 840 432 747 268 1626
Fragments arranged by size:
1626 840 747 432 292 268
EcoNI CCTnn'n_nnAGG
Cuts at: 478 478
Size: 4205
EcoO109I rG'GnC_Cy
Cuts at: 436 483 902 1344 436
Size: 47 419 442 3297
Fragments arranged by size:
3297 442 419 47
EcoRI G'AATT_C
Cuts at: 1009 1209 1009
Size: 200 4005
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 4205
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4205
HaeII r_GCGC'y
Cuts at: 1067 1639 1887 2909 3888 1067
Size: 572 248 1022 979 1384
Fragments arranged by size:
1384 1022 979 572 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 260 292 2047 2077 2079 2109 260
Size: 32 1755 30 2 30 2356
Fragments arranged by size:
2356 1755 32 30 30 2
HincII GTy'rAC
Cuts at: 336 1244 336
Size: 908 3297
KasI G'GCGC_C
Cuts at: 1635 1883 1635
Size: 248 3957
KpnI G_GTAC'C
Cuts at: 1225 1225
Size: 4205
MluI A'CGCG_T
Cuts at: 3638 3638
Size: 4205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 719 2876 3060 719
Size: 2157 184 1864
Fragments arranged by size:
2157 1864 184
MscI TGG'CCA
Cuts at: 304 517 631 1966 304
Size: 213 114 1335 2543
Fragments arranged by size:
2543 1335 213 114
MslI CAynn'nnrTG
Cuts at: 160 580 637 1181 2194 2321 160
Size: 420 57 544 1013 127 2044
Fragments arranged by size:
2044 1013 544 420 127 57
MspA1I CmG'CkG
Cuts at: 1316 1630 1990 3003 3248 1316
Size: 314 360 1013 245 2273
Fragments arranged by size:
2273 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 4205
NarI GG'CG_CC
Cuts at: 1636 1884 1636
Size: 248 3957
NcoI C'CATG_G
Cuts at: 107 107
Size: 4205
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 4205
NspI r_CATG'y
Cuts at: 567 1258 1758 2665 567
Size: 691 500 907 2107
Fragments arranged by size:
2107 907 691 500
PciI A'CATG_T
Cuts at: 2661 2661
Size: 4205
PflMI CCAn_nnn'nTGG
Cuts at: 239 1194 239
Size: 955 3250
PfoI T'CCnGG_A
Cuts at: 924 924
Size: 4205
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 4173
PpuMI rG'GwC_Cy
Cuts at: 436 436
Size: 4205
PshAI GACnn'nnGTC
Cuts at: 886 886
Size: 4205
PsiI TTA'TAA
Cuts at: 209 209
Size: 4205
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 758 790 758
Size: 32 4173
PstI C_TGCA'G
Cuts at: 390 1252 1937 390
Size: 862 685 2658
Fragments arranged by size:
2658 862 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4205
PvuII CAG'CTG
Cuts at: 1630 1990 1630
Size: 360 3845
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 1219 1219
Size: 4205
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 2228 2438 2228
Size: 210 3995
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SfcI C'TryA_G
Cuts at: 33 386 711 1248 1933 2926 3117 33
Size: 353 325 537 685 993 191 1121
Fragments arranged by size:
1121 993 685 537 353 325 191
SfoI GGC'GCC
Cuts at: 1637 1885 1637
Size: 248 3957
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 1227 1227
Size: 4205
SmlI C'TyrA_G
Cuts at: 1360 2275 2767 3029 1360
Size: 915 492 262 2536
Fragments arranged by size:
2536 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 567 1258 1758 567
Size: 691 500 3014
Fragments arranged by size:
3014 691 500
StyI C'CwwG_G
Cuts at: 107 1339 1693 107
Size: 1232 354 2619
Fragments arranged by size:
2619 1232 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 631 1788 1921 2380 2589 631
Size: 1157 133 459 209 2247
Fragments arranged by size:
2247 1157 459 209 133
TatI w'GTAC_w
Cuts at: 3720 3720
Size: 4205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4173
Tth111I GACn'n_nGTC
Cuts at: 2002 2002
Size: 4205
XbaI T'CTAG_A
Cuts at: 945 1236 945
Size: 291 3914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 747 1700 747
Size: 953 3252
XmaI C'CCGG_G
Cuts at: 1225 1225
Size: 4205
XmnI GAAnn'nnTTC
Cuts at: 991 991
Size: 4205
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI