Restriction map for
pF1KE0613
(Circular) (Six-base) MAPSORT of: pF1KE0613.seq Check: 3461 from: 1 to: 3629
pF1KE0613 3629 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 607 667 607
Size: 60 3569
Acc65I G'GTAC_C
Cuts at: 645 645
Size: 3629
AclI AA'CG_TT
Cuts at: 3469 3469
Size: 3629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 1454 1886 2633 2901 407
Size: 1047 432 747 268 1135
Fragments arranged by size:
1135 1047 747 432 268
AflII C'TTAA_G
Cuts at: 477 477
Size: 3629
AflIII A'CryG_T
Cuts at: 981 2085 3062 981
Size: 1104 977 1548
Fragments arranged by size:
1548 1104 977
AgeI A'CCGG_T
Cuts at: 594 2017 594
Size: 1423 2206
AlwNI CAG_nnn'CTG
Cuts at: 411 934 2501 411
Size: 523 1567 1539
Fragments arranged by size:
1567 1539 523
ApaLI G'TGCA_C
Cuts at: 2399 2399
Size: 3629
ApoI r'AATT_y
Cuts at: 142 494 633 2982 3238 142
Size: 352 139 2349 256 533
Fragments arranged by size:
2349 533 352 256 139
AseI AT'TA_AT
Cuts at: 20 20
Size: 3629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
AvaI C'yCGr_G
Cuts at: 649 649
Size: 3629
BamHI G'GATC_C
Cuts at: 654 654
Size: 3629
BanI G'GyrC_C
Cuts at: 645 1059 1307 1342 645
Size: 414 248 35 2932
Fragments arranged by size:
2932 414 248 35
BanII G_rGCy'C
Cuts at: 329 643 1673 329
Size: 314 1030 2285
Fragments arranged by size:
2285 1030 314
BbeI G_GCGC'C
Cuts at: 1063 1311 1063
Size: 248 3381
BbsI GAAGACnn'nnnn_
Cuts at: 355 365 395 355
Size: 10 30 3589
Fragments arranged by size:
3589 30 10
BciVI GTATCCnnnnn_n'
Cuts at: 1527 2288 3629 1527
Size: 761 1341 1527
Fragments arranged by size:
1527 1341 761
BclI T'GATC_A
Cuts at: 299 1149 299
Size: 850 2779
BglII A'GATC_T
Cuts at: 1144 3181 1144
Size: 2037 1592
BlpI GC'TnA_GC
Cuts at: 179 741 179
Size: 562 3067
Bme1580I G_kGCm'C
Cuts at: 1254 1347 2403 1254
Size: 93 1056 2480
Fragments arranged by size:
2480 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 498 841 922 1257 3401 498
Size: 343 81 335 2144 726
Fragments arranged by size:
2144 726 343 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 331 134
Size: 197 3432
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 805 1684 2176 2474 484
Size: 321 879 492 298 1639
Fragments arranged by size:
1639 879 492 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 3333 3333
Size: 3629
BsaAI yAC'GTr
Cuts at: 982 982
Size: 3629
BsaBI GATnn'nnATC
Cuts at: 6 1143 3180 6
Size: 1137 2037 455
Fragments arranged by size:
2037 1137 455
BsaHI Gr'CG_yC
Cuts at: 1060 1308 2010 3506 1060
Size: 248 702 1496 1183
Fragments arranged by size:
1496 1183 702 248
BsaWI w'CCGG_w
Cuts at: 594 818 1339 2017 2291 2438 2849 594
Size: 224 521 678 274 147 411 1374
Fragments arranged by size:
1374 678 521 411 274 224 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 214 244 214
Size: 30 3599
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 3629
BseYI C'CCAG_C
Cuts at: 174 1054 1812 2389 2885 174
Size: 880 758 577 496 918
Fragments arranged by size:
918 880 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1171 1217 2425 104
Size: 1067 46 1208 1308
Fragments arranged by size:
1308 1208 1067 46
BsiHKAI G_wGCw'C
Cuts at: 643 1421 1611 2403 643
Size: 778 190 792 1869
Fragments arranged by size:
1869 792 778 190
BsmI GAATG_Cn'
Cuts at: 121 169 121
Size: 48 3581
Bsp1286I G_dGCh'C
Cuts at: 329 643 1254 1347 1421 1611 1673 2403 329
Size: 314 611 93 74 190 62 730 1555
Fragments arranged by size:
1555 730 611 314 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2849 2849
Size: 3629
BspHI T'CATG_A
Cuts at: 2805 2805
Size: 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 679 893 1195 1576 679
Size: 214 302 381 2732
Fragments arranged by size:
2732 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1921 1975 3459 3624 50
Size: 1871 54 1484 165 55
Fragments arranged by size:
1871 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 1541
Size: 3629
BsrFI r'CCGG_y
Cuts at: 594 1627 1808 2017 594
Size: 1033 181 209 2206
Fragments arranged by size:
2206 1033 209 181
BssSI C'ACGA_G
Cuts at: 1900 2258 1900
Size: 358 3271
BstAPI GCAn_nnn'nTGC
Cuts at: 411 1006 411
Size: 595 3034
BstBI TT'CG_AA
Cuts at: 1990 1990
Size: 3629
BstXI CCAn_nnnn'nTGG
Cuts at: 158 158
Size: 3629
BstYI r'GATC_y
Cuts at: 1 654 1121 1144 1479 1725 2737 2823 2835
Size: 653 467 23 335 246 1012 86 12
Cuts at: 2835 3181 1
Size: 346 449
Fragments arranged by size:
1012 653 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 553 553
Size: 3629
BtgI C'CryG_G
Cuts at: 107 107
Size: 3629
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1579 1760 1923 1579
Size: 181 163 3285
Fragments arranged by size:
3285 181 163
BtsI GCAGTG_nn'
Cuts at: 872 872
Size: 3629
DraI TTT'AAA
Cuts at: 628 628
Size: 3629
DrdI GACnn_nn'nnGTC
Cuts at: 1335 2193 1335
Size: 858 2771
EaeI y'GGCC_r
Cuts at: 1214 1388 1779 1806 3151 1214
Size: 174 391 27 1345 1692
Fragments arranged by size:
1692 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1214 1214
Size: 3629
EarI CTCTTCn'nnn_
Cuts at: 1652 1862 1652
Size: 210 3419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1837 2147 2293 3439 1837
Size: 310 146 1146 2027
Fragments arranged by size:
2027 1146 310 146
EcoICRI GAG'CTC
Cuts at: 641 641
Size: 3629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 331 407 1454 1886 2633 2901 134
Size: 197 76 1047 432 747 268 862
Fragments arranged by size:
1047 862 747 432 268 197 76
EcoNI CCTnn'n_nnAGG
Cuts at: 238 238
Size: 3629
EcoO109I rG'GnC_Cy
Cuts at: 408 768 408
Size: 360 3269
EcoRI G'AATT_C
Cuts at: 142 633 142
Size: 491 3138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 529 1648 529
Size: 1119 2510
FspI TGC'GCA
Cuts at: 1410 1410
Size: 3629
HaeII r_GCGC'y
Cuts at: 1063 1311 2333 3312 1063
Size: 248 1022 979 1380
Fragments arranged by size:
1380 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 291 323 1471 1501 1503 1533 291
Size: 32 1148 30 2 30 2387
Fragments arranged by size:
2387 1148 32 30 30 2
HincII GTy'rAC
Cuts at: 608 668 608
Size: 60 3569
KasI G'GCGC_C
Cuts at: 1059 1307 1059
Size: 248 3381
KpnI G_GTAC'C
Cuts at: 649 649
Size: 3629
MluI A'CGCG_T
Cuts at: 3062 3062
Size: 3629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2300 2484 2300
Size: 184 3445
MscI TGG'CCA
Cuts at: 1390 1390
Size: 3629
MslI CAynn'nnrTG
Cuts at: 1618 1745 1618
Size: 127 3502
MspA1I CmG'CkG
Cuts at: 183 740 1054 1414 2427 2672 183
Size: 557 314 360 1013 245 1140
Fragments arranged by size:
1140 1013 557 360 314 245
NaeI GCC'GGC
Cuts at: 1810 1810
Size: 3629
NarI GG'CG_CC
Cuts at: 1060 1308 1060
Size: 248 3381
NcoI C'CATG_G
Cuts at: 107 107
Size: 3629
NgoMIV G'CCGG_C
Cuts at: 1808 1808
Size: 3629
NspI r_CATG'y
Cuts at: 580 682 1182 2089 580
Size: 102 500 907 2120
Fragments arranged by size:
2120 907 500 102
PciI A'CATG_T
Cuts at: 2085 2085
Size: 3629
PmeI GTTT'AAAC
Cuts at: 628 628
Size: 3629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2768 2800 2768
Size: 32 3597
PstI C_TGCA'G
Cuts at: 676 1361 676
Size: 685 2944
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3629
PvuII CAG'CTG
Cuts at: 1054 1414 1054
Size: 360 3269
RsrII CG'GwC_CG
Cuts at: 1824 1824
Size: 3629
SacI G_AGCT'C
Cuts at: 643 643
Size: 3629
SalI G'TCGA_C
Cuts at: 606 666 606
Size: 60 3569
SapI GCTCTTCn'nnn_
Cuts at: 1652 1862 1652
Size: 210 3419
SbfI CC_TGCA'GG
Cuts at: 676 676
Size: 3629
ScaI AGT'ACT
Cuts at: 3146 3146
Size: 3629
SfcI C'TryA_G
Cuts at: 33 216 672 1357 2350 2541 33
Size: 183 456 685 993 191 1121
Fragments arranged by size:
1121 993 685 456 191 183
SfoI GGC'GCC
Cuts at: 1061 1309 1061
Size: 248 3381
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
SmaI CCC'GGG
Cuts at: 651 651
Size: 3629
SmlI C'TyrA_G
Cuts at: 463 477 784 1699 2191 2453 463
Size: 14 307 915 492 262 1639
Fragments arranged by size:
1639 915 492 307 262 14
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3629
SphI G_CATG'C
Cuts at: 682 1182 682
Size: 500 3129
StuI AGG'CCT
Cuts at: 558 558
Size: 3629
StyI C'CwwG_G
Cuts at: 107 234 763 1117 107
Size: 127 529 354 2619
Fragments arranged by size:
2619 529 354 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1212 1345 1804 2013 1212
Size: 133 459 209 2828
Fragments arranged by size:
2828 459 209 133
TatI w'GTAC_w
Cuts at: 3144 3144
Size: 3629
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3597
Tth111I GACn'n_nGTC
Cuts at: 1426 1426
Size: 3629
XbaI T'CTAG_A
Cuts at: 138 660 138
Size: 522 3107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1124 114
Size: 1010 2619
XmaI C'CCGG_G
Cuts at: 649 649
Size: 3629
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI