Restriction map for
pF1KE0611
(Circular) (Six-base) MAPSORT of: pF1KE0611.seq Check: 5838 from: 1 to: 3629
pF1KE0611 3629 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 344 667 344
Size: 323 3306
Acc65I G'GTAC_C
Cuts at: 383 645 383
Size: 262 3367
AclI AA'CG_TT
Cuts at: 3469 3469
Size: 3629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1454 1886 2633 2901 227
Size: 1227 432 747 268 955
Fragments arranged by size:
1227 955 747 432 268
AflIII A'CryG_T
Cuts at: 981 2085 3062 981
Size: 1104 977 1548
Fragments arranged by size:
1548 1104 977
AgeI A'CCGG_T
Cuts at: 485 2017 485
Size: 1532 2097
AlwNI CAG_nnn'CTG
Cuts at: 171 934 2501 171
Size: 763 1567 1299
Fragments arranged by size:
1567 1299 763
ApaLI G'TGCA_C
Cuts at: 2399 2399
Size: 3629
ApoI r'AATT_y
Cuts at: 633 2982 3238 633
Size: 2349 256 1024
Fragments arranged by size:
2349 1024 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
AvaI C'yCGr_G
Cuts at: 245 573 649 245
Size: 328 76 3225
Fragments arranged by size:
3225 328 76
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 375 408 375
Size: 33 3596
BamHI G'GATC_C
Cuts at: 654 654
Size: 3629
BanI G'GyrC_C
Cuts at: 383 645 1059 1307 1342 383
Size: 262 414 248 35 2670
Fragments arranged by size:
2670 414 262 248 35
BanII G_rGCy'C
Cuts at: 574 643 1673 574
Size: 69 1030 2530
Fragments arranged by size:
2530 1030 69
BbeI G_GCGC'C
Cuts at: 1063 1311 1063
Size: 248 3381
BbsI GAAGACnn'nnnn_
Cuts at: 174 174
Size: 3629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 341 375 341
Size: 34 3595
BciVI GTATCCnnnnn_n'
Cuts at: 1527 2288 3629 1527
Size: 761 1341 1527
Fragments arranged by size:
1527 1341 761
BclI T'GATC_A
Cuts at: 1149 1149
Size: 3629
BglI GCCn_nnn'nGGC
Cuts at: 301 301
Size: 3629
BglII A'GATC_T
Cuts at: 200 241 1144 3181 200
Size: 41 903 2037 648
Fragments arranged by size:
2037 903 648 41
BlpI GC'TnA_GC
Cuts at: 741 741
Size: 3629
Bme1580I G_kGCm'C
Cuts at: 1254 1347 2403 1254
Size: 93 1056 2480
Fragments arranged by size:
2480 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 841 922 1257 3401 841
Size: 81 335 2144 1069
Fragments arranged by size:
2144 1069 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 504 193
Size: 311 3318
Bpu10I CC'TnA_GC
Cuts at: 287 287
Size: 3629
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1684 2176 2474 805
Size: 879 492 298 1960
Fragments arranged by size:
1960 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 226 3333 226
Size: 3107 522
BsaAI yAC'GTr
Cuts at: 982 982
Size: 3629
BsaBI GATnn'nnATC
Cuts at: 6 1143 3180 6
Size: 1137 2037 455
Fragments arranged by size:
2037 1137 455
BsaHI Gr'CG_yC
Cuts at: 1060 1308 2010 3506 1060
Size: 248 702 1496 1183
Fragments arranged by size:
1496 1183 702 248
BsaWI w'CCGG_w
Cuts at: 485 566 818 1339 2017 2291 2438 2849 485
Size: 81 252 521 678 274 147 411 1265
Fragments arranged by size:
1265 678 521 411 274 252 147 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 502 532 534 564 502
Size: 30 2 30 3567
Fragments arranged by size:
3567 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 470 473 470
Size: 3 3626
BseYI C'CCAG_C
Cuts at: 1054 1812 2389 2885 1054
Size: 758 577 496 1798
Fragments arranged by size:
1798 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1171 1217 2425 104
Size: 1067 46 1208 1308
Fragments arranged by size:
1308 1208 1067 46
BsiHKAI G_wGCw'C
Cuts at: 574 643 1421 1611 2403 574
Size: 69 778 190 792 1800
Fragments arranged by size:
1800 792 778 190 69
Bsp1286I G_dGCh'C
Cuts at: 574 643 1254 1347 1421 1611 1673 2403 574
Size: 69 611 93 74 190 62 730 1800
Fragments arranged by size:
1800 730 611 190 93 74 69 62
BspEI T'CCGG_A
Cuts at: 2849 2849
Size: 3629
BspHI T'CATG_A
Cuts at: 2805 2805
Size: 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 168 679 893 1195 1576 168
Size: 511 214 302 381 2221
Fragments arranged by size:
2221 511 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1921 1975 3459 3624 50
Size: 1871 54 1484 165 55
Fragments arranged by size:
1871 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 1541
Size: 3629
BsrFI r'CCGG_y
Cuts at: 485 1627 1808 2017 485
Size: 1142 181 209 2097
Fragments arranged by size:
2097 1142 209 181
BssSI C'ACGA_G
Cuts at: 1900 2258 1900
Size: 358 3271
BstAPI GCAn_nnn'nTGC
Cuts at: 1006 1006
Size: 3629
BstBI TT'CG_AA
Cuts at: 1990 1990
Size: 3629
BstYI r'GATC_y
Cuts at: 1 200 241 335 654 1121 1144 1479 1725
Size: 199 41 94 319 467 23 335 246
Cuts at: 1725 2737 2823 2835 3181 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 319 246 199
94 86 41 23 12
BtgI C'CryG_G
Cuts at: 107 378 387 420 441 107
Size: 271 9 33 21 3295
Fragments arranged by size:
3295 271 33 21 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1579 1760 1923 1579
Size: 181 163 3285
Fragments arranged by size:
3285 181 163
BtsI GCAGTG_nn'
Cuts at: 872 872
Size: 3629
DraI TTT'AAA
Cuts at: 628 628
Size: 3629
DrdI GACnn_nn'nnGTC
Cuts at: 1335 2193 1335
Size: 858 2771
EaeI y'GGCC_r
Cuts at: 302 1214 1388 1779 1806 3151 302
Size: 912 174 391 27 1345 780
Fragments arranged by size:
1345 912 780 391 174 27
EagI C'GGCC_G
Cuts at: 1214 1214
Size: 3629
EarI CTCTTCn'nnn_
Cuts at: 1652 1862 1652
Size: 210 3419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1837 2147 2293 3439 1837
Size: 310 146 1146 2027
Fragments arranged by size:
2027 1146 310 146
EcoICRI GAG'CTC
Cuts at: 572 641 572
Size: 69 3560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 227 504 1454 1886 2633 2901 193
Size: 34 277 950 432 747 268 921
Fragments arranged by size:
950 921 747 432 277 268 34
EcoO109I rG'GnC_Cy
Cuts at: 581 768 581
Size: 187 3442
EcoRI G'AATT_C
Cuts at: 633 633
Size: 3629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 145 1648 145
Size: 1503 2126
FspI TGC'GCA
Cuts at: 1410 1410
Size: 3629
HaeII r_GCGC'y
Cuts at: 1063 1311 2333 3312 1063
Size: 248 1022 979 1380
Fragments arranged by size:
1380 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 320 352 358 390 556 588 1471 1501 1503
Size: 32 6 32 166 32 883 30 2
Cuts at: 1503 1533 320
Size: 30 2416
Fragments arranged by size:
2416 883 166 32 32 32 30 30
6 2
HincII GTy'rAC
Cuts at: 668 668
Size: 3629
KasI G'GCGC_C
Cuts at: 1059 1307 1059
Size: 248 3381
KpnI G_GTAC'C
Cuts at: 387 649 387
Size: 262 3367
MluI A'CGCG_T
Cuts at: 3062 3062
Size: 3629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 117 2300 2484 117
Size: 2183 184 1262
Fragments arranged by size:
2183 1262 184
MscI TGG'CCA
Cuts at: 304 1390 304
Size: 1086 2543
MslI CAynn'nnrTG
Cuts at: 1618 1745 1618
Size: 127 3502
MspA1I CmG'CkG
Cuts at: 740 1054 1414 2427 2672 740
Size: 314 360 1013 245 1697
Fragments arranged by size:
1697 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1810 1810
Size: 3629
NarI GG'CG_CC
Cuts at: 1060 1308 1060
Size: 248 3381
NcoI C'CATG_G
Cuts at: 107 107
Size: 3629
NgoMIV G'CCGG_C
Cuts at: 1808 1808
Size: 3629
NspI r_CATG'y
Cuts at: 682 1182 2089 682
Size: 500 907 2222
Fragments arranged by size:
2222 907 500
PciI A'CATG_T
Cuts at: 2085 2085
Size: 3629
PfoI T'CCnGG_A
Cuts at: 496 496
Size: 3629
PmeI GTTT'AAAC
Cuts at: 628 628
Size: 3629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2768 2800 2768
Size: 32 3597
PpuMI rG'GwC_Cy
Cuts at: 581 581
Size: 3629
PstI C_TGCA'G
Cuts at: 676 1361 676
Size: 685 2944
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3629
PvuII CAG'CTG
Cuts at: 1054 1414 1054
Size: 360 3269
RsrII CG'GwC_CG
Cuts at: 1824 1824
Size: 3629
SacI G_AGCT'C
Cuts at: 574 643 574
Size: 69 3560
SalI G'TCGA_C
Cuts at: 666 666
Size: 3629
SapI GCTCTTCn'nnn_
Cuts at: 1652 1862 1652
Size: 210 3419
SbfI CC_TGCA'GG
Cuts at: 676 676
Size: 3629
ScaI AGT'ACT
Cuts at: 3146 3146
Size: 3629
SfcI C'TryA_G
Cuts at: 33 320 531 558 585 672 1357 2350 2541
Size: 287 211 27 27 87 685 993 191
Cuts at: 2541 33
Size: 1121
Fragments arranged by size:
1121 993 685 287 211 191 87 27
27
SfoI GGC'GCC
Cuts at: 1061 1309 1061
Size: 248 3381
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
SmaI CCC'GGG
Cuts at: 651 651
Size: 3629
SmlI C'TyrA_G
Cuts at: 784 1699 2191 2453 784
Size: 915 492 262 1960
Fragments arranged by size:
1960 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3629
SphI G_CATG'C
Cuts at: 682 1182 682
Size: 500 3129
StyI C'CwwG_G
Cuts at: 107 763 1117 107
Size: 656 354 2619
Fragments arranged by size:
2619 656 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 482 1212 1345 1804 2013 482
Size: 730 133 459 209 2098
Fragments arranged by size:
2098 730 459 209 133
TatI w'GTAC_w
Cuts at: 3144 3144
Size: 3629
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 534 566 3146 3178 534
Size: 32 2580 32 985
Fragments arranged by size:
2580 985 32 32
Tth111I GACn'n_nGTC
Cuts at: 1426 1426
Size: 3629
XbaI T'CTAG_A
Cuts at: 451 660 451
Size: 209 3420
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1124 1124
Size: 3629
XmaI C'CCGG_G
Cuts at: 649 649
Size: 3629
XmnI GAAnn'nnTTC
Cuts at: 487 487
Size: 3629
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BfrBI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI