Restriction map for
pF1KE0610
(Circular) (Six-base) MAPSORT of: pF1KE0610.seq Check: 3751 from: 1 to: 4202
pF1KE0610 4202 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 983 983
Size: 4202
AccI GT'mk_AC
Cuts at: 1240 1240
Size: 4202
Acc65I G'GTAC_C
Cuts at: 1218 1218
Size: 4202
AclI AA'CG_TT
Cuts at: 1133 4042 1133
Size: 2909 1293
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2459 3206 3474 2027
Size: 432 747 268 2755
Fragments arranged by size:
2755 747 432 268
AflIII A'CryG_T
Cuts at: 1554 2658 3635 1554
Size: 1104 977 2121
Fragments arranged by size:
2121 1104 977
AgeI A'CCGG_T
Cuts at: 2590 2590
Size: 4202
AlwNI CAG_nnn'CTG
Cuts at: 619 1507 3074 619
Size: 888 1567 1747
Fragments arranged by size:
1747 1567 888
ApaLI G'TGCA_C
Cuts at: 2972 2972
Size: 4202
ApoI r'AATT_y
Cuts at: 333 1206 3555 3811 333
Size: 873 2349 256 724
Fragments arranged by size:
2349 873 724 256
AseI AT'TA_AT
Cuts at: 20 1090 20
Size: 1070 3132
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
AvaI C'yCGr_G
Cuts at: 1222 1222
Size: 4202
BamHI G'GATC_C
Cuts at: 1227 1227
Size: 4202
BanI G'GyrC_C
Cuts at: 1218 1632 1880 1915 1218
Size: 414 248 35 3505
Fragments arranged by size:
3505 414 248 35
BanII G_rGCy'C
Cuts at: 1216 2246 1216
Size: 1030 3172
BbeI G_GCGC'C
Cuts at: 1636 1884 1636
Size: 248 3954
BciVI GTATCCnnnnn_n'
Cuts at: 2100 2861 4202 2100
Size: 761 1341 2100
Fragments arranged by size:
2100 1341 761
BclI T'GATC_A
Cuts at: 327 1722 327
Size: 1395 2807
BglII A'GATC_T
Cuts at: 1717 3754 1717
Size: 2037 2165
BlpI GC'TnA_GC
Cuts at: 1314 1314
Size: 4202
Bme1580I G_kGCm'C
Cuts at: 1827 1920 2976 1827
Size: 93 1056 3053
Fragments arranged by size:
3053 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 492 1414 1495 1830 3974 492
Size: 922 81 335 2144 720
Fragments arranged by size:
2144 922 720 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 567 2569 567
Size: 2002 2200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 612 665 567
Size: 45 53 4104
Fragments arranged by size:
4104 53 45
Bpu10I CC'TnA_GC
Cuts at: 990 990
Size: 4202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 1378 2257 2749 3047 710
Size: 668 879 492 298 1865
Fragments arranged by size:
1865 879 668 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 492 652 849 946 3906 492
Size: 160 197 97 2960 788
Fragments arranged by size:
2960 788 197 160 97
BsaAI yAC'GTr
Cuts at: 1555 1555
Size: 4202
BsaBI GATnn'nnATC
Cuts at: 6 1716 3753 6
Size: 1710 2037 455
Fragments arranged by size:
2037 1710 455
BsaHI Gr'CG_yC
Cuts at: 1633 1881 2583 4079 1633
Size: 248 702 1496 1756
Fragments arranged by size:
1756 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1391 1912 2590 2864 3011 3422 1391
Size: 521 678 274 147 411 2171
Fragments arranged by size:
2171 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 472 502 472
Size: 30 4172
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 207 556 749 1136 207
Size: 349 193 387 3273
Fragments arranged by size:
3273 387 349 193
BseYI C'CCAG_C
Cuts at: 262 1627 2385 2962 3458 262
Size: 1365 758 577 496 1006
Fragments arranged by size:
1365 1006 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 4202
BsiEI CG_ry'CG
Cuts at: 104 1744 1790 2998 104
Size: 1640 46 1208 1308
Fragments arranged by size:
1640 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1216 1994 2184 2976 1216
Size: 778 190 792 2442
Fragments arranged by size:
2442 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1216 1827 1920 1994 2184 2246 2976 1216
Size: 611 93 74 190 62 730 2442
Fragments arranged by size:
2442 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3422 3422
Size: 4202
BspHI T'CATG_A
Cuts at: 3378 3378
Size: 4202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 983 1252 1466 1768 2149 983
Size: 269 214 302 381 3036
Fragments arranged by size:
3036 381 302 269 214
BsrBI CCG'CTC
Cuts at: 50 2494 2548 4032 4197 50
Size: 2444 54 1484 165 55
Fragments arranged by size:
2444 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 791 2114 791
Size: 1323 2879
BsrFI r'CCGG_y
Cuts at: 2200 2381 2590 2200
Size: 181 209 3812
Fragments arranged by size:
3812 209 181
BssSI C'ACGA_G
Cuts at: 2473 2831 2473
Size: 358 3844
BstAPI GCAn_nnn'nTGC
Cuts at: 381 1579 381
Size: 1198 3004
BstBI TT'CG_AA
Cuts at: 2563 2563
Size: 4202
BstXI CCAn_nnnn'nTGG
Cuts at: 847 847
Size: 4202
BstYI r'GATC_y
Cuts at: 1 1227 1694 1717 2052 2298 3310 3396 3408
Size: 1226 467 23 335 246 1012 86 12
Cuts at: 3408 3754 1
Size: 346 449
Fragments arranged by size:
1226 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2152 2333 2496 2152
Size: 181 163 3858
Fragments arranged by size:
3858 181 163
BtsI GCAGTG_nn'
Cuts at: 1445 1445
Size: 4202
DraI TTT'AAA
Cuts at: 396 1201 396
Size: 805 3397
DrdI GACnn_nn'nnGTC
Cuts at: 1908 2766 1908
Size: 858 3344
EaeI y'GGCC_r
Cuts at: 456 1787 1961 2352 2379 3724 456
Size: 1331 174 391 27 1345 934
Fragments arranged by size:
1345 1331 934 391 174 27
EagI C'GGCC_G
Cuts at: 1787 1787
Size: 4202
EarI CTCTTCn'nnn_
Cuts at: 121 580 734 2225 2435 121
Size: 459 154 1491 210 1888
Fragments arranged by size:
1888 1491 459 210 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2410 2720 2866 4012 2410
Size: 310 146 1146 2600
Fragments arranged by size:
2600 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1214 1214
Size: 4202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 612 665 2027 2459 3206 3474 567
Size: 45 53 1362 432 747 268 1295
Fragments arranged by size:
1362 1295 747 432 268 53 45
EcoNI CCTnn'n_nnAGG
Cuts at: 256 256
Size: 4202
EcoO109I rG'GnC_Cy
Cuts at: 966 1341 966
Size: 375 3827
EcoRI G'AATT_C
Cuts at: 1206 1206
Size: 4202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 4202
FspI TGC'GCA
Cuts at: 1983 1983
Size: 4202
HaeII r_GCGC'y
Cuts at: 1636 1884 2906 3885 1636
Size: 248 1022 979 1953
Fragments arranged by size:
1953 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 264 296 403 435 472 504 829 861 2044
Size: 32 107 32 37 32 325 32 1183
Cuts at: 2044 2074 2076 2106 264
Size: 30 2 30 2360
Fragments arranged by size:
2360 1183 325 107 37 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1241 1241
Size: 4202
KasI G'GCGC_C
Cuts at: 1632 1880 1632
Size: 248 3954
KpnI G_GTAC'C
Cuts at: 1222 1222
Size: 4202
MfeI C'AATT_G
Cuts at: 1078 1078
Size: 4202
MluI A'CGCG_T
Cuts at: 3635 3635
Size: 4202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 761 2873 3057 761
Size: 2112 184 1906
Fragments arranged by size:
2112 1906 184
MscI TGG'CCA
Cuts at: 458 1963 458
Size: 1505 2697
MslI CAynn'nnrTG
Cuts at: 407 2191 2318 407
Size: 1784 127 2291
Fragments arranged by size:
2291 1784 127
MspA1I CmG'CkG
Cuts at: 210 1313 1627 1987 3000 3245 210
Size: 1103 314 360 1013 245 1167
Fragments arranged by size:
1167 1103 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2383 2383
Size: 4202
NarI GG'CG_CC
Cuts at: 1633 1881 1633
Size: 248 3954
NcoI C'CATG_G
Cuts at: 107 107
Size: 4202
NgoMIV G'CCGG_C
Cuts at: 2381 2381
Size: 4202
NspI r_CATG'y
Cuts at: 1255 1755 2662 1255
Size: 500 907 2795
Fragments arranged by size:
2795 907 500
PciI A'CATG_T
Cuts at: 2658 2658
Size: 4202
PflMI CCAn_nnn'nTGG
Cuts at: 643 643
Size: 4202
PfoI T'CCnGG_A
Cuts at: 681 681
Size: 4202
PmeI GTTT'AAAC
Cuts at: 1201 1201
Size: 4202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 4170
PpuMI rG'GwC_Cy
Cuts at: 966 966
Size: 4202
PsiI TTA'TAA
Cuts at: 293 293
Size: 4202
PstI C_TGCA'G
Cuts at: 1249 1934 1249
Size: 685 3517
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4202
PvuII CAG'CTG
Cuts at: 210 1627 1987 210
Size: 1417 360 2425
Fragments arranged by size:
2425 1417 360
RsrII CG'GwC_CG
Cuts at: 2397 2397
Size: 4202
SacI G_AGCT'C
Cuts at: 1216 1216
Size: 4202
SalI G'TCGA_C
Cuts at: 1239 1239
Size: 4202
SapI GCTCTTCn'nnn_
Cuts at: 2225 2435 2225
Size: 210 3992
SbfI CC_TGCA'GG
Cuts at: 1249 1249
Size: 4202
ScaI AGT'ACT
Cuts at: 3719 3719
Size: 4202
SexAI A'CCwGG_T
Cuts at: 839 839
Size: 4202
SfcI C'TryA_G
Cuts at: 33 1245 1930 2923 3114 33
Size: 1212 685 993 191 1121
Fragments arranged by size:
1212 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1634 1882 1634
Size: 248 3954
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
SmaI CCC'GGG
Cuts at: 1224 1224
Size: 4202
SmlI C'TyrA_G
Cuts at: 725 1357 2272 2764 3026 725
Size: 632 915 492 262 1901
Fragments arranged by size:
1901 915 632 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4202
SphI G_CATG'C
Cuts at: 1255 1755 1255
Size: 500 3702
StyI C'CwwG_G
Cuts at: 107 594 1336 1690 107
Size: 487 742 354 2619
Fragments arranged by size:
2619 742 487 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1785 1918 2377 2586 1785
Size: 133 459 209 3401
Fragments arranged by size:
3401 459 209 133
TatI w'GTAC_w
Cuts at: 3717 3717
Size: 4202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 298 330 3719 3751 298
Size: 32 3389 32 749
Fragments arranged by size:
3389 749 32 32
Tth111I GACn'n_nGTC
Cuts at: 484 841 1999 484
Size: 357 1158 2687
Fragments arranged by size:
2687 1158 357
XbaI T'CTAG_A
Cuts at: 1233 1233
Size: 4202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1697 1697
Size: 4202
XmaI C'CCGG_G
Cuts at: 1222 1222
Size: 4202
XmnI GAAnn'nnTTC
Cuts at: 1135 1135
Size: 4202
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI