Restriction map for
pF1KE0608
(Circular) (Six-base) MAPSORT of: pF1KE0608.seq Check: 8544 from: 1 to: 4190
pF1KE0608 4190 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1228 1228
Size: 4190
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 912 2015 2447 3194 3462 912
Size: 1103 432 747 268 1640
Fragments arranged by size:
1640 1103 747 432 268
AflIII A'CryG_T
Cuts at: 895 1542 2646 3623 895
Size: 647 1104 977 1462
Fragments arranged by size:
1462 1104 977 647
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 750 750
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 1495 3062 1495
Size: 1567 2623
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 380 1135 1194 3543 3799 380
Size: 755 59 2349 256 771
Fragments arranged by size:
2349 771 755 256 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 266 1210 266
Size: 944 3246
BamHI G'GATC_C
Cuts at: 1039 1215 1039
Size: 176 4014
BanI G'GyrC_C
Cuts at: 922 1206 1620 1868 1903 922
Size: 284 414 248 35 3209
Fragments arranged by size:
3209 414 284 248 35
BanII G_rGCy'C
Cuts at: 862 1204 2234 862
Size: 342 1030 2818
Fragments arranged by size:
2818 1030 342
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 557 1710 557
Size: 1153 3037
BglII A'GATC_T
Cuts at: 685 1705 3742 685
Size: 1020 2037 1133
Fragments arranged by size:
2037 1133 1020
BlpI GC'TnA_GC
Cuts at: 856 1302 856
Size: 446 3744
Bme1580I G_kGCm'C
Cuts at: 1815 1908 2964 1815
Size: 93 1056 3041
Fragments arranged by size:
3041 1056 93
BmgBI CAC'GTC
Cuts at: 896 896
Size: 4190
BmrI ACTGGGnnnn_n'
Cuts at: 734 1402 1483 1818 3962 734
Size: 668 81 335 2144 962
Fragments arranged by size:
2144 962 668 335 81
BmtI G_CTAG'C
Cuts at: 244 244
Size: 4190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 897 897
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 329 3894 329
Size: 3565 625
BsaAI yAC'GTr
Cuts at: 1543 1543
Size: 4190
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2571 4067 1621
Size: 248 702 1496 1744
Fragments arranged by size:
1744 1496 702 248
BsaWI w'CCGG_w
Cuts at: 568 815 1013 1379 1900 2578 2852 2999 3410
Size: 247 198 366 521 678 274 147 411
Cuts at: 3410 568
Size: 1348
Fragments arranged by size:
1348 678 521 411 366 274 247 198
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 107 137 1008 1038 107
Size: 30 871 30 3259
Fragments arranged by size:
3259 871 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 408 1005 408
Size: 597 3593
BseYI C'CCAG_C
Cuts at: 166 462 801 1615 2373 2950 3446 166
Size: 296 339 814 758 577 496 910
Fragments arranged by size:
910 814 758 577 496 339 296
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 523 862 1204 1982 2172 2964 523
Size: 339 342 778 190 792 1749
Fragments arranged by size:
1749 792 778 342 339 190
BsmI GAATG_Cn'
Cuts at: 414 414
Size: 4190
Bsp1286I G_dGCh'C
Cuts at: 523 862 1204 1815 1908 1982 2172 2234 2964
Size: 339 342 611 93 74 190 62 730
Cuts at: 2964 523
Size: 1749
Fragments arranged by size:
1749 730 611 342 339 190 93 74
62
BspEI T'CCGG_A
Cuts at: 568 3410 568
Size: 2842 1348
BspHI T'CATG_A
Cuts at: 3366 3366
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 584 633 1240 1454 1756 2137 584
Size: 49 607 214 302 381 2637
Fragments arranged by size:
2637 607 381 302 214 49
BsrBI CCG'CTC
Cuts at: 50 2482 2536 4020 4185 50
Size: 2432 54 1484 165 55
Fragments arranged by size:
2432 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 147 2102 147
Size: 1955 2235
BsrFI r'CCGG_y
Cuts at: 199 2188 2369 2578 199
Size: 1989 181 209 1811
Fragments arranged by size:
1989 1811 209 181
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 870 870
Size: 4190
BstYI r'GATC_y
Cuts at: 1 685 1039 1215 1682 1705 2040 2286 3298
Size: 684 354 176 467 23 335 246 1012
Cuts at: 3298 3384 3396 3742 1
Size: 86 12 346 449
Fragments arranged by size:
1012 684 467 449 354 346 335 246
176 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2140 2321 2484 2140
Size: 181 163 3846
Fragments arranged by size:
3846 181 163
BtsI GCAGTG_nn'
Cuts at: 1433 1433
Size: 4190
ClaI AT'CG_AT
Cuts at: 822 822
Size: 4190
DraI TTT'AAA
Cuts at: 650 1189 650
Size: 539 3651
DrdI GACnn_nn'nnGTC
Cuts at: 900 1896 2754 900
Size: 996 858 2336
Fragments arranged by size:
2336 996 858
EaeI y'GGCC_r
Cuts at: 1775 1949 2340 2367 3712 1775
Size: 174 391 27 1345 2253
Fragments arranged by size:
2253 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 1093 1160 2213 2423 1093
Size: 67 1053 210 2860
Fragments arranged by size:
2860 1053 210 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 169 2398 2708 2854 4000 169
Size: 2229 310 146 1146 359
Fragments arranged by size:
2229 1146 359 310 146
EcoICRI GAG'CTC
Cuts at: 860 1202 860
Size: 342 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 897 912 2015 2447 3194 3462 897
Size: 15 1103 432 747 268 1625
Fragments arranged by size:
1625 1103 747 432 268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 794 794
Size: 4190
EcoO109I rG'GnC_Cy
Cuts at: 395 1085 1329 395
Size: 690 244 3256
Fragments arranged by size:
3256 690 244
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1146 2209 1146
Size: 1063 3127
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 1624 1872 2894 3873 1624
Size: 248 1022 979 1941
Fragments arranged by size:
1941 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 576 608 670 702 754 786 2032 2062 2064
Size: 32 62 32 52 32 1246 30 2
Cuts at: 2064 2094 576
Size: 30 2672
Fragments arranged by size:
2672 1246 62 52 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 552 1229 552
Size: 677 3513
HindIII A'AGCT_T
Cuts at: 334 334
Size: 4190
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2861 3045 2861
Size: 184 4006
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4190
MslI CAynn'nnrTG
Cuts at: 722 868 2179 2306 722
Size: 146 1311 127 2606
Fragments arranged by size:
2606 1311 146 127
MspA1I CmG'CkG
Cuts at: 842 1037 1301 1615 1975 2988 3233 842
Size: 195 264 314 360 1013 245 1799
Fragments arranged by size:
1799 1013 360 314 264 245 195
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NheI G'CTAG_C
Cuts at: 240 240
Size: 4190
NspI r_CATG'y
Cuts at: 1080 1243 1743 2650 1080
Size: 163 500 907 2620
Fragments arranged by size:
2620 907 500 163
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PpuMI rG'GwC_Cy
Cuts at: 1085 1085
Size: 4190
PstI C_TGCA'G
Cuts at: 644 695 1237 1922 644
Size: 51 542 685 2912
Fragments arranged by size:
2912 685 542 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 842 1615 1975 842
Size: 773 360 3057
Fragments arranged by size:
3057 773 360
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 862 1204 862
Size: 342 3848
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SanDI GG'GwC_CC
Cuts at: 1085 1085
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 1093 2213 2423 1093
Size: 1120 210 2860
Fragments arranged by size:
2860 1120 210
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SfcI C'TryA_G
Cuts at: 33 640 691 1233 1918 2911 3102 33
Size: 607 51 542 685 993 191 1121
Fragments arranged by size:
1121 993 685 607 542 191 51
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 1345 2260 2752 3014 1345
Size: 915 492 262 2521
Fragments arranged by size:
2521 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StyI C'CwwG_G
Cuts at: 968 1324 1678 968
Size: 356 354 3480
Fragments arranged by size:
3480 356 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 663 1773 1906 2365 2574 663
Size: 1110 133 459 209 2279
Fragments arranged by size:
2279 1110 459 209 133
TatI w'GTAC_w
Cuts at: 680 943 3705 680
Size: 263 2762 1165
Fragments arranged by size:
2762 1165 263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4158
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI